Non-Nitrogen-Fixers or Nitrogen-Fixers? Factors Distinguishing the Dominance of Chroococcal and Diazotrophic Cyanobacterial Species.

Global warming and eutrophication are the main factors driving the development of cyanobacterial dominance in aquatic ecosystems. We used a model linking water temperature, oxygen saturation, concentrations of PO43-, NO3-, NH4+, total dissolved iron (TDFe), and SO42- to cyanobacteria to test the turnover patterns of cyanobacterial dominance of non-nitrogen-fixing (chroococcal species) and nitrogen-fixing (filamentous diazotrophic) species. Statistical analysis was performed using decision trees. The dominance patterns of the two morphologically and ecologically distinct cyanobacterial species were associated with different environmental factors. However, SO42- was the most important factor that explained whether non-nitrogen-fixing or nitrogen-fixing species would dominate. Other important factors were water temperature, phosphate concentration, and oxygen saturation. The model for dominance of non-nitrogen-fixing species used SO42-, PO43-, and water temperature (upper layers), and SO42-, the ratio of PO43-/NH4+, and oxygen saturation (bottom layers). In contrast, water temperature, SO42-, and NH4+ in the upper layers and SO42-, NH4+, and water temperature in the bottom layers were used for the dominance of nitrogen-fixing species. The dominance of Aphanizomenon flos-aquae was explained by different sets of variables, indicating the presence of different strains of this species. The other cyanobacteria species showed dominance patterns that could be explained by one set of variables. As cyanobacterial blooms proliferate due to climate change, it is important to know which factors, in addition to phosphorus and nitrogen, are crucial for the mass development of the various cyanobacterial species.

Interactions between Ciliate Species and Aphanizomenon flos-aquae Vary Depending on the Morphological Form and Biomass of the Diazotrophic Cyanobacterium.

Aphanizomenon flos-aquae can form extensive blooms from freshwater to the brackish environment and, being a diazotrophic species, contribute significantly to the nitrogen and carbon cycle. It occurs as single filaments or aggregates and could be used as an alternative nutrients source for bacteria and ciliates. Ciliates are a group of organisms playing a crucial role in the transfer of nitrogen from primary producers to higher trophic levels in aquatic food webs. The aim of the experiment was to study the effects of the cyanobacterium A. flos-aquae on the community of five ciliate species (Spirostomum minus, Euplotes aediculatus, Strobilidium sp., Vorticella sp. and Paramecium tetraurelia). The response of each species to the presence of a low/high cyanobacterial biomass and to the different morphological forms of A. flos-aquae (single filaments or aggregates) was demonstrated. The results of the experiment showed the variability of interactions between the cyanobacterium A. flos-aquae and ciliates and pointed out the possible benefits that A. flos-aquae provides to the ciliates (e.g., a substrate for the development of bacteria as food for ciliates or as a source of nitrogen and carbon).

Variations in zooplankton functional groups density in freshwater ecosystems exposed to cyanobacterial blooms.

The recently observed decline of biodiversity has become a major problem on Earth. Anthropogenically-driven climate change and eutrophication of natural habitats represent great threats to both terrestrial and aquatic ecosystems. Particularly vulnerable are freshwater ecosystems, where the mentioned changes promote development of cyanobacteria. Extension of the cyanobacterial bloom period, increase of cyanobacterial biomass production and toxicity may involve numerous changes in freshwater ecosystem functioning. We conducted a study in water bodies exposed to cyanobacterial blooms of different duration: short-lasting and long-lasting cyanobacterial blooms. We tested the impact of cyanobacterial biomass on densities of zooplankton species grouped into functional groups (functional groups densities) under conditions of different bloom length periods. The functional groups of zooplankton were identified on the basis of three functional traits of each species: mean body length (BL), potential food source (FS) and feeding type (FT). The results of the study showed that assembling species into functional groups may be helpful in understanding the interactions between biotic components of ecosystem. The results showed that the response of each functional group density for cyanobacterial biomass development depends on the functional traits of the animals (i.e. how the species interact with the whole food web) and may be different depending on the bloom intensity, which acts like a factor selecting adapted species.

Microcystins affect zooplankton biodiversity in oxbow lakes.

The authors tested the hypothesis that zooplankton diversity and density are affected by the presence of cyanotoxins in the water. The authors focused on 4 oxbow lakes of the Vistula River in southern Poland, which are subjected to mass cyanobacterial development. In 2 of the oxbows (Piekary and Tyniec), microcystins released into the water were found. The highest concentration of microcystins (0.246 µg/L) was observed for microcystins LR. Zooplankton diversity showed a weak response to the presence of microcystins released into the water. The Shannon index (H') of total zooplankton diversity decreased in the Piekary and Tyniec oxbows during periods when the microcystin concentrations were highest. The same trend was noted for diversity of rotifers in both oxbows and for diversity of copepods in Piekary, but not for copepods in Tyniec. No such trends were found for the diversity of cladocerans in any of the oxbows, nor was a relationship found between density of zooplankton and microcystins. Statistical analyses showed that the number of species in individual samples was negatively correlated with the levels of sulfates, phosphates, and ammonia, but the microcystin concentration was positively related to those levels. This points to the complexity of the interactions and synergies among toxins, abiotic factors, and zooplankton biodiversity. In focusing on the problem of cyanotoxins, conservation studies should pay attention to this complexity. Environ Toxicol Chem 2017;36:165-174. © 2016 SETAC.