The Levels of Cortisol and Selected Biochemical Parameters in Red Deer Harvested during Stalking Hunts.

As a reactive species, the red deer is sensitive to both negative exogenous and endogenous stimuli. An intensive hunting period may have a particularly negative impact on game animals. The aim of this study was to determine the plasma cortisol level and biochemical parameters in 25 wild red deer (Cervus elaphus) harvested during stalking hunts in correlation with the sex and age of the animals. The mean cortisol concentrations in the stags and hinds analyzed in this study were similar (20.2 and 21.5 ng/mL, respectively). Higher HDL cholesterol values were found in the blood of the hinds than in stags (p < 0.05). Similarly, the mean levels of LDL cholesterol, lactate dehydrogenase, and alanine aminotransferase were higher by 21%, 16%, and 42%, respectively, in the blood of the hinds. In contrast, the levels of alkaline phosphatase, bilirubin, and aspartate aminotransferase were higher in the stags (by 30%, 49%, and 36%, respectively). There was a negative correlation of the cortisol concentration with urea and bilirubin and a positive correlation between cortisol and aspartate aminotransferase in the stags (p < 0.05). In turn, a negative correlation was found between the cortisol and urea levels in the hinds (p < 0.05). In summary, the stress caused by stalking hunts and the characteristic behavior of red deer during the mating season had an impact on chosen biochemical parameters. The increased concentration of cortisol resulted in a decrease in the carcass mass, which may lead to the deterioration of the physical condition of animals on hunting grounds.

Equine Asthma Does Not Affect Circulating Myostatin Concentrations in Horses.

(1) Background: The number of horses suffering from chronic respiratory diseases, resembling human asthma, is increasing but there is still a lack of reliable and accurate methods to detect these disorders. Numerous studies have found elevated plasma concentrations of one of the myokines, namely, myostatin (MSTN), in people suffering from severe asthma. MSTN normally inhibits myoblast proliferation and differentiation through autocrine or paracrine signals. Therefore, given the pathogenesis of asthma, we hypothesize that MSTN could be a useful biomarker of equine asthma. Thus, this study aimed to compare the concentration of MSTN in the blood plasma of fully healthy and asthmatic horses. (2) Methods: A total of 61 horses were clinically examined to confirm or exclude the occurrence of equine asthma, including bronchoalveolar lavage (BAL) fluid cytology performed on 49 horses. This study included three groups of horses, two of which were clinically healthy, and one of which was asthmatic. (3) Results: The mean circulatory MSTN concentration determined using the ELISA method in asthmatic horses was significantly higher than that in clinically healthy young Thoroughbred racehorses (p < 0.05), but it did not differ as compared to the group of healthy, adult leisure horses. (4) Conclusions: The obtained results did not unambiguously support our original hypothesis that MSTM may be a reliable marker for the early diagnosis of equine asthma. To the best of the authors' knowledge, this is the first study to analyze the plasma MSTN concentration in equine asthma patients, and therefore further studies are needed to confirm our novel findings.

Plasma relaxin, estrogens and progestogens concentration in coldblooded and hot-blood mares in the second half of gestation.

Monitoring estrogens, progestogens, and relaxin plasma concentration can be important for determining pregnancies at risk of failure in mares. However, significant breed-related differences in values of reproductive hormones are known, and data concerning coldblooded (CB) mares are missing. The aim of the study was to determine and compare serum relaxin, estrone sulphate (E1S), estradiol-17ß (E2), and progestogens concentration in pregnant CB and hot-blood (HB) mares during the second half of gestation. Blood samples obtained from 94 healthy mares in the second half of gestation with known mating dates were analyzed. Samples were collected from 36 CB mares aged from 3 to 16 years and 58 HB mares aged from 4 to 17 years. The hormone concentrations were measured using immunoenzymatic equine species-specific commercial kits. The serum relaxin concentration was almost three times lower in CB, whereas E1S and E2 almost twice as low, as compared to HB mares (P < 0.05). In conclusion, the extremely low concentration of relaxin in CB mares could be one reason for the increased amounts of difficult parturition requiring veterinary intervention noted in these breeds.

Physiological response of roe deer (Capreolus capreolus) during stalking hunts depending on age.

BACKGROUND: The European roe deer (Capreolus capreolus) is a species particularly reactive to all kinds of negative stimuli. Hunting activity is one of the most potent stressors that disturbs the welfare of wild animals. During stress, various endocrine responses are elicited to improve the physical performance of the affected individual. A commonly assessed hormone for overcoming stressful situations is cortisol (CORT). In this study, plasma CORT levels in roe deer were assessed during the season of the most intense stalking hunts in Poland (summer vs. late autumn), the sex of the harvested animals (males vs. females), and age of harvest animal. In addition, the health status of the roe doe was evaluated on the basis of selected indices of blood chemistry, which could be associated with circulating cortisol levels. RESULTS: The mean cortisol levels were 58.066 ng/ml in the male group (summer) and 27.694 ng/ml in the female group (late autumn). Higher CORT levels were associated with a significantly lower of total cholesterol, lactate dehydrogenase, and uric acid (p < 0.05). Moreover, the mean concentration of uric acid was negatively correlated with the level of CORT in the male and female groups (p < 0.05). Together with the increase in mean CORT level, the HDL cholesterol of all the tested animals increased significantly (p < 0.05). CONCLUSIONS: Higher CORT in males during the summer than in females during the late autumn were most likely due to the arousal with the mating season. The level of CORT increased with the animals' age. Uric acid and age are both predictors of roe deer's serum CORT level.

The Influence of Exercise Intensity on Tryptophan Metabolites in Thoroughbred Horses.

Catabolism of tryptophan (Trp) is modulated by physical activity and provides a pool of active compounds: Trp is considered a calmative agent, kynurenine (Kyn) and 3-hydroxykynurenine (3-HKyn) show neurotoxic effects, kynurenic acid (Kyna) and xanthurenic acid (XA) have neuroprotective properties like nicotinamide (NAm), while serotonin is the neurotransmitter. The study was conducted to investigate the dependence of exercise intensity, measured by plasma lactic acid (LA) concentration, on the level of Trp, its catabolites (serotonin, Kyn, 3-HKyn, Kyna and XA), and NAm in Thoroughbred horses. A total of 18 young race Thoroughbred horses were investigated during exercise tests. Blood samples for analysis were collected: at rest, 10 min after the end of the exercise, and 60 min after the end of the exercise. Plasma LA was determined by the enzymatic method, Trp, and other metabolites using liquid chromatography coupled with mass spectrometry. In horses performing intense exercise, the concentration of LA, Kyn, XA and NAm was increased, while Trp was decreased. Significant correlations were detected for exercise-induced increase in LA and 3-HKyn, XA, and NAm. Considering the scope of changes in analyzed data, there is an expected neutral effect on the health status of exercised horses.

The Concentration of Selected Heavy Metals in Muscles, Liver and Kidneys of Pigs Fed Standard Diets and Diets Containing 60% of New Rye Varieties.

The carry-over of heavy metals from feed to muscles is generally low if animals are fed with a standard diet containing amounts below the maximum permissible levels. However, prolonged exposure to heavy metals can lead to their accumulation in some organs like muscles, liver, and kidneys. This paves the way for human health risks related to the consumption of products of animal-origin. Thus, using feed mixtures with a low level of heavy metals in pig production will contribute to increasing public health and safety and is of environmental concern. The study aimed to assess the impact of the level of some heavy metals (Cd, Pb, Hg, Cu, Zn, Fe and Mn) in standard (control) feed mixtures and in alternative feed mixtures based on maize or new rye varieties (population and hybrid) on the heavy metal concentration in muscles, liver and kidney of fattened pigs at slaughter. While some differences between heavy metals content in examined tissue samples from experimental groups were observed, all of them were in the range of allowable levels according to European Community rules. In conclusion, new rye varieties, especially the hybrid variety, could be an alternative source of cereal grains for pig nutrition.

Plasma Activin A and Decorin in Exercised Purebred Arabian Horses - Preliminary Study.

Development of the musculoskeletal system occurs, in part, through an impact on regulatory proteins, such as activin A and decorin. Activin A induces atrophic effects on skeletal muscle, and decorin regulates the formation of connective tissue proteins like collagen and elastin in tendons and ligaments. The aim of this study was to evaluate the influence of different intensities of exercise on blood plasma activin A and decorin concentrations in horses. Ten young purebred Arabian horses were subjected to routine training sessions on a racetrack, and another 11 adult horses of the same breed participated in endurance rides. Race horses were examined during gallop tests over a distance of 1200 m and endurance horses at distances from 20 to 80 km. Blood samples were collected at rest and after exercise to determine plasma concentrations of activin A, decorin and cortisol. Despite differences in the intensity of exercise, the plasma decorin concentration remained unchanged, and activin A tended to decrease in endurance horses only. The exercise-induced changes in plasma activin A concentrations correlated with the covered distance (r = -0.43; P < .05), but not with the changes in cortisol values. Further studies are needed to confirm the usefulness of plasma activin A as a potential indicator of a horse's endurance performance.

Concentrations of Circulating Irisin and Myostatin in Race and Endurace Purebred Arabian Horses-Preliminary Study.

Skeletal muscle is considered to be the largest endocrine organ determining the maintenance of energy homeostasis. Adaptive changes in skeletal muscles in response to physical exercise influence the production as well as secretion of myokines, which are bioactive factors that play a crucial role in energy expenditure processes. The aim of the study was to investigate the impact of two different types of exercise on the circulating level of two of these, myostatin and irisin, in trained horses. Twenty purebred Arabian horses were involved in the study: 10 three-year-old horses trained on the racetrack and 10 endurance horses aged 7.4 ± 1.9 years. The horses from both groups were regularly trained throughout the entire season, during which they also participated in Polish National competitions. To assess the influence of the training sessions on plasma myostatin and irisin concentrations, blood samples taken at rest and 30 min after the end of exercise were analyzed. In the studied horses, the single bout of exercise did not influence plasma irisin but induced an increase in plasma myostatin concentration. In racehorses, plasma irisin concentration decreased with the length of the training season. Plasma myostatin was higher in endurance horses than in three-year-old racehorses. Lack of exercise-induced fluctuation in circulating irisin in studied horses suggests that myostatin released in response to exercise provides a negative feedback signal to irisin release.

Plasma Apelin Concentration in Exercised Horses: Preliminary Study.

Physical effort is one of the key aspects of keeping horses in good condition. The condition of the animal is reflected by multiple blood parameters. The newly discovered cytokine-apelin can pose one of them, however, so far, has not been studied in the horse population. Apelin is produced by adipocytes and myocytes and plays an important role in the energy metabolism of the body through the influence, for example, on the process of adipogenesis and lipolysis. The aim of this study was to investigate if physical effort of various intensity affects the plasma concentration of apelin in horses. The study involved 20 purebred Arabian horses divided into two groups. The first group included 10 race horses, aged 3 years, and second group included 10 horses aged from 6 to 12 years, used in endurance rides. Blood samples were collected from each horse at rest and after exercise. The concentrations of apelin, lactic acid, cortisol, uric acid (UA), triacylglycerols, total plasma protein, and glucose were determined in plasma samples. Race training sessions induced significant decreases in plasma apelin concentrations (P < .05). In endurance horses, significant correlations were found only between exercise-induced changes of apelin and cortisol (r = 0.55) and apelin and UA (r = 0.67) concentrations. In conclusion, the concentration of apelin in equine plasma decreases in response to short-time exercise unlike endurance exercise. Blood plasma apelin concentration determined at rest is lower in long-time regularly trained horses than in the ones at the beginning of their training process.

Gut-bone axis response to dietary replacement of soybean meal with raw low-tannin faba bean seeds in broiler chickens.

It seems that faba bean (FB) seeds could be a good protein-energy component in animal feed, but the presence of anti-nutritional substances limits their use as a substitute of soybean meal. The aim of the study was to examine the influence of different concentrations of raw, low-tannin, FB seeds on the gut-bone axis in Ross 308 broilers. One-day old chickens were randomly subjected to one of the 3 dietary treatments: the control group was fed standard diet based on soybean meal and without FB seeds, and two groups were fed 8%/15% and 16%/22% of raw low-tannin FB seeds in the starter and grower, respectively. On the 35th day, hematological and serum biochemical analyses as well histomorphometry of the small intestine and liver tissue and bone mechanical tests were performed. The diet type had no effect on the body weight gain and feed conversion ratio. However, the basal intestinal structures were significantly reduced in birds fed the lower concentration of FB. The enlargement of nerve plexuses was dependent on the concentration used in the diet and, additionally, on the kind of plexus and location in the intestinal tract. The liver was characterized by an increase in non-hepatocytes. There was no influence of the low-tannin FB seeds on most of the analyzed serum parameters in the 35-day-old broiler chickens, except the decreased concentration of total cholesterol and Ca in both experimental groups, triglycerides in group I, and P and uric acid in group II. Furthermore, the increasing concentration of the dietary low-tannin FB did not influence the activities of AspAT (except the group fed the higher amount of FB), ALAT, and LDH. The broiler chickens had no visible leg lesions and no problem in the locomotor function, but the tibiae were lighter mainly in birds fed the higher concentration of FB seeds. Geometric analysis revealed reduction of the cross section area and wall thickness, indicating a decline in the bone midshaft, which influenced the densitometric parameters and the results of mechanical tests. In conclusion, the dietary inclusion of raw low-tannin FB seeds had no negative effects on broiler growth, but disturbed the intestine structure and tibia characteristics. Therefore, all these negative effects necessitate additional examinations before inclusion of raw low-tannin FB seed into poultry diet.

The effect of relaxing massage on heart rate and heart rate variability in purebred Arabian racehorses.

The objective of this study was to assess the effect of relaxing massage on the heart rate (HR) and heart rate variability (HRV) in young racehorses during their first racing season. In the study, 72 Purebred Arabian racehorses were included. The study was implemented during the full race season. The horses from control and experimental groups were included in regular race training 6 days a week. The horses from the experimental group were additionally subject to the relaxing massage 3 days a week during the whole study. HR and HRV were assumed as indicators of the emotional state of the horses. The measurements were taken six times, every 4-5 weeks. The HRV parameters were measured at rest, during grooming and saddling the horse and during warm-up walking under a rider. The changes of the parameters throughout the season suggest that the relaxing massage may be effectively used to make the racehorses more relaxed and calm. Moreover, the horses from the experimental group had better race performance records.

Changes in salivary and plasma cortisol levels in Purebred Arabian horses during race training session.

Physical activity and stress both cause an increase in cortisol release ratio. The aim of this study was to evaluate the use of saliva samples for the determination of cortisol concentrations indicating the work-load level in horses during race training. Twelve Purebred Arabian horses aged 3-5 years were studied during the routine training session. After the warm-up, the horses galloped on the 800 m sand track at a speed of 12.8 m/s. Three saliva samples, and three blood samples were collected from each horse. Both types of samples were taken at rest, immediately after return from the track and after 30 min restitution. The concentrations of blood lactic acid (LA), and cortisol in saliva and plasma samples were measured and analyzed. Blood LA, plasma and salivary cortisol levels increased significantly after exercise (P < 0.05). Salivary cortisol concentration determined 30 min after the exercise correlated significantly with plasma cortisol level obtained immediately after exercise (P < 0.05) as well as measured 30 min after the end of exercise (P < 0.05). The determination of cortisol concentration in saliva samples taken from racehorses 30 min after the end of exercise can be recommended to use in field conditions to estimate the work-load in racehorses.

Blood phagocyte activity after race training sessions in Thoroughbred and Arabian horses.

Intensive exercise and exertion during competition promote many changes that may result in the impairment of immunity and increased susceptibility to infections. The aim of this study was to evaluate the activity of "the first line of defense": neutrophils and monocytes in racing Thoroughbred and Arabian horses after routine training sessions. Twenty-three (12 Thoroughbred and 11 Arabian) horses were examined. Routine haematological (number of red blood cells - RBC, haemoglobin concentration - HGB, haematocrit - HCT, total number of white blood cells - WBC), biochemical (creatine phosphokinase activity - CPK and total protein concentration - TP) parameters, cortisol concentration as well as phagocytic and oxidative burst activity of neutrophils and monocytes were determined. The values of basic parameters and the activity of phagocytes differed between breeds and distinct patterns of exercise-induced changes were observed. The training sessions did not produce the decrease in phagocyte activity that might lead to the suppression of immunity.

[Using pedicled mandibular osteomuscular flap in orbital reconstruction]. / Rekonstrukcja dna oczodolu wyrostkiem dziobiastym zuchwy uszypulowanym na miesniu skroniowym.

Represented the way of orbital floor reconstruction using coronoid process of the mandible pedicled on temporal muscle. The clinical evaluation of vascularised bone graft from coronoid process confirmed their usefulness in the face recon struction surgery.

[Breathing disorders after resection of structures in the oral cavity, throat and neck studied by pulse oximetry examinations]. / Zaburzenia oddychania po rozlegllych zabiegach operacyjnych w jamie ustnej, gardle i szyi na podstawie wyników badán pulsoksymetrycznych.

INTRODUCTION: Surgical treatment of oral cavity neoplasms consists of the resection of tumor surrounded by a healthy tissue margin. This study is aimed at an objective, qualitative and quantitative evaluation of breathing disturbances and dyspnoea after the resection of oral cavity structures, throat structures and muscles of the neck. For the purpose of evaluating the above-mentioned disturbances an attempt was made to carry out pulse oximetry examination. MATERIAL AND METHODS: We have examined 50 persons after the resection of oral cavity structures, throat structures and muscles of the neck in Maxillofacial Clinic of Pomeranian Medical Academy in Szczecin. The examinations were performed by pulse oximetry set during sleep before and after operations. The patients were divided into three groups according to the type of removed structures which included: lateral part of the mandible; mental part of the mandible; part of the tongue. RESULTS: The results of examinations obtained gave rise results as: pulse oximetry examination revealed an increased incidence of blood desaturation episodes and a decrease in minimum saturation level. CONCLUSIONS: Surgical treatment of oral cavity neoplasms leads to breathing disturbances. Apnoea episodes cause a drop in the arterial blood saturation with oxygen.

[Nose reconstruction in material of Maxillofacial Surgery Department at the Pomeranian Academy of Medicine in Szczecin in 1975-2004]. / Rekonstrukcja nosa w materiale Kliniki Chirurgii Szczekowo-Twarzowej PAM w latach 1975-2004.

INTRODUCTION: The focus of this paper is the evaluation of usability of various nose skin reconstruction methods applied after removal of a skin cancer in the Teaching Hospital of Maxillofacial Surgery at the Pomeranian Academy of Medicine in Szczecin in 1975-2004. MATERIAL AND METHODS: The research material consisted of 285 patients who underwent a surgical treatment of nose skin cancers located in various skin areas. In 67 cases, the tumour was situated on a nostril flare, in 42--on the nose tip, in 98--on the bridge and in 68--on one of its sides, most frequently, near the corner of the eye. Said tumours often covered more than one of the above-mentioned areas or even all of them. Reconstruction planning and performance was an integral part of the surgical treatment. The tumour amputation was preceded by an assessment of the quantity and quality of tissues necessary for filling in the lesions. Lesions located on the nose surface were covered by free skin grafts. In the case of nostril flare tumours, they were filled in with grafts composed of skin and cartilaginous tissue from the ear. Lesions including all nose layers were most frequently (in 136 cases) covered with various types of axially vascularised frontal skin flaps i.e. by the middle or temporal part of the frontal flap. In the case of elderly people the nasolabial flaps were used. RESULTS: In most cases, both the aesthetic and functional effects were very good. What appeared to be the most difficult reconstruction case was the restoration of the nasal bone structures after their complete amputation. CONCLUSION: Due to the thickness, colour and easiness of preparation of the middle frontal flap, it is considered to be the best material for reconstruction of the nose.

[Observations of diagnosis and treatment of the salivary gland tumors]. / Spostrzezenia dotyczace rozpoznawania i leczenia nowotworów slinianek.

Morphological and imaging methods of diagnosis and therapy of salivary gland tumors were presented. They have changed in period of thirty years. The accuracy of pretreatment diagnostic methods increased from 40 to 90%. Wrong diagnoses repeated in cases: adenoid cystic carcinoma, mucoepidermoid carcinoma and metastases from other organs. Their frequency in our material was 21.2%. Changes in treatment of pleomorphic adenoma and adenoid cystic carcinoma were described. Percentage of 5-years survival was 64% in irradiated after tumor excision all treated. The patient's active participation in functional rehabilitation after facial nerve reconstruction was accentuated.