Disarming the defenses: Insect detoxification of plant defense-related specialized metabolites.

The ability of certain insects to feed on plants containing toxic specialized metabolites may be attributed to detoxification enzymes. Representatives of a few large families of detoxification enzymes are widespread in insect herbivores acting to functionalize toxins and conjugate them with polar substituents to decrease toxicity, increase water solubility and enhance excretion. Insects have also developed specific enzymes for coping with toxins that are activated upon plant damage. Another source of detoxification potential in insects lies in their microbiomes, which are being increasingly recognized for their role in processing plant toxins. The evolution of insect detoxification systems to resist toxic specialized metabolites in plants may in turn have selected for the great diversity of such metabolites found in nature.

Functions of stone cells and oleoresin terpenes in the conifer defense syndrome.

Conifers depend on complex defense systems against herbivores. Stone cells (SC) and oleoresin are physical and chemical defenses of Sitka spruce that have been separately studied in previous work. Weevil oviposit at the tip of the previous year's apical shoot (PYAS). We investigated interactions between weevil larvae and trees in controlled oviposition experiments with resistant (R) and susceptible (S) Sitka spruce. R trees have an abundance of SC in the PYAS cortex. SC are mostly absent in S trees. R trees and S trees also differ in the composition of oleoresin terpenes. Transcriptomes of R and S trees revealed differences in long-term weevil-induced responses. Performance of larvae was significantly reduced on R trees compared with S trees under experimental conditions that mimicked natural oviposition behavior at apical shoot tips and may be attributed to the effects of SC. In oviposition experiments designed for larvae to feed below the area of highest SC abundance, larvae showed an unusual feeding behavior and oleoresin appeared to function as the major defense. The results support a role for both SC and oleoresin terpenes and possible synergies between these traits in the defense syndrome of weevil-resistant Sitka spruce.