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1.
Cureus ; 16(7): e65868, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-39219937

RESUMEN

Minimally invasive surgery (MIS) provides superior results in the surgical treatment of inflammatory bowel disease (IBD). There exist various minimally invasive procedures, each possessing its own set of benefits and drawbacks. This literature review outlines these methodologies and underscores their importance in enhancing the outcomes of patients with IBD. A grand total of 192 studies were carefully chosen and succinctly summarized. Conventional multiport laparoscopy is the most widely used MIS for IBD, with single-incision laparoscopy showing even better results. Robotic surgery offers comparable results but at higher costs and longer operation times. In the future, there will be widespread acceptance of single-incision laparoscopy and robotic surgery due to improved training and reduced expenses. Further research into the technology's utility in different IBD presentations could increase its usage.

2.
Plast Reconstr Surg ; 2024 Aug 23.
Artículo en Inglés | MEDLINE | ID: mdl-39212965

RESUMEN

BACKGROUND: Various surgical subspecialties, including plastic surgery, have begun to embrace virtual clinic visits, especially since the COVID-19 pandemic. However, the impact of video visits on time optimization and cost incurred in outpatient plastic surgery clinics has not been studied. METHODS: Using the time-driven activity-based costing (TDABC) method, we examined the time and cost of in-person and virtual visits at an academic plastic surgery clinic. We formulated process maps for four visit types: physician-led in-person, physician assistant-led in-person, physician-led virtual, and physician assistant-led virtual. The time associated with each visit type was generated by direct observation. The cost associated with each visit type was calculated from representative salary information and estimation of resource costs. RESULTS: Virtual visits took on average less time (25.3 minutes for physician-led visits and 24.4 minutes for physician assistant-led visits), compared to in-person visits (48.2 minutes for physician-led and 41.1 minutes for physician-assistant-led visits) (p<0.001). Virtual visits were also cheaper, at $52.80 for physician-led visits and $20.70 for physician assistant-led visits, compared to in-person visits ($261.13 for physician-led and $236.00 for physician assistant-led visits). Non-provider activities made up the majority of traditional in-person visits (75.7% of the visit for physician-led and 77.6% for physician assistant-led visits), which contributed to higher overall cost of in-person visits for both groups of providers. CONCLUSIONS: Virtual clinic visits can produce time and cost savings without reducing the amount of face-to-face time between providers and patients. Virtual visits can be a useful adjunct to traditional in-person visits.

3.
bioRxiv ; 2024 Aug 12.
Artículo en Inglés | MEDLINE | ID: mdl-39211094

RESUMEN

Brain-machine interface (BMI) controlled functional electrical stimulation (FES) is a promising treatment to restore hand movements to people with cervical spinal cord injury. Recent intracortical BMIs have shown unprecedented successes in decoding user intentions, however the hand movements restored by FES have largely been limited to predetermined grasps. Restoring dexterous hand movements will require continuous control of many biomechanically linked degrees-of-freedom in the hand, such as wrist and finger flexion, that would form the basis of those movements. Here we investigate the ability to restore simultaneous wrist and finger flexion, which would enable grasping with a controlled hand posture and assist in manipulating objects once grasped. We demonstrate that intramuscular FES can enable monkeys with temporarily paralyzed hands to move their fingers and wrist across a functional range of motion, spanning an average 88.6 degrees at the metacarpophalangeal joint flexion and 71.3 degrees of wrist flexion, and intramuscular FES can control both joints simultaneously in a real-time task. Additionally, we demonstrate a monkey using an intracortical BMI to control the wrist and finger flexion in a virtual hand, both before and after the hand is temporarily paralyzed, even achieving success rates and acquisition times equivalent to able-bodied control with BMI control after temporary paralysis in two sessions. Together, this outlines a method using an artificial brain-to-body interface that could restore continuous wrist and finger movements after spinal cord injury.

4.
J Phys Chem B ; 128(32): 7912-7919, 2024 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-39105702

RESUMEN

Enhancing molecular self-assembly at the monolayer level offers significant potential for various applications. For monolayers made of π-conjugated discotic liquid crystal (DLC) molecule nanowires, achieving precise separation and alignment of these nanowires has been a long-standing challenge. This research explores an approach using the manipulation of subphase temperature and surface pressure within a Langmuir trough to control molecular nanowire separation. We observe notable temperature-dependent behavior: as the temperature increases from 5 to 30 °C, the monolayer collapse pressure rises steadily. In contrast, temperatures from 35 to 50 °C exhibit an initial small plateau with a nonzero slope that becomes more distinct with rising temperature. Our study of Langmuir-Blodgett (LB) films provides crucial insights into the monolayer's structure. At lower temperatures, the LB films show coalesced molecular nanowires, whereas at higher temperatures, the DLC nanowires separate and form an interconnected network. Remarkably, upon compression, this network transforms into a compact, highly uniform monolayer. To explain these temperature-dependent behaviors, we examine the area relaxation curves, which indicate a two-step molecular loss mechanism involving desorption and monolayer collapse due to the nucleation and growth of critical nuclei. This extensive study offers valuable insights into the dynamic interaction of the temperature, surface pressure, and molecular assembly, enhancing our understanding of the fundamental processes in monolayer self-assembly.

5.
J Evol Biol ; 2024 Aug 06.
Artículo en Inglés | MEDLINE | ID: mdl-39105302

RESUMEN

Locomotor activity is one of the major traits that is affected by age. Greater locomotor activity is also known to evolve in the course of dispersal evolution. However, the impact of dispersal evolution on the functional senescence of locomotor activity is largely unknown. We addressed this knowledge gap using large outbred populations of Drosophila melanogaster selected for increased dispersal. We tracked locomotor activity of these flies at regular intervals until a late age. Longevity of these flies was also recorded. We found that locomotor activity declines with age in general. However interestingly, activity level of dispersal selected populations never drops below the ancestry-matched-controls, despite the rate of age-dependent decline in activity of the dispersal selected populations being greater than their respective controls. Dispersal selected population was also found to have shorter lifespan as compared to its control, a potential cost of elevated level of activity throughout their life. These results are crucial in the context of invasion biology as contemporary climate change, habitat degradation, and destruction provide congenial conditions for dispersal evolution. Such controlled and tractable studies investigating the ageing pattern of important functional traits are important in the field of biogerontology as well.

6.
Open Life Sci ; 19(1): 20220903, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39027422

RESUMEN

Catfish (Clarias magur) is a popular freshwater fish food worldwide. The processing of this fish generates a significant amount of waste, mainly in the form of viscera, which constitutes around 10-12% of the fish's total weight. This study was focused on extracting polyunsaturated fatty acid (PUFA)-rich oil from catfish viscera, aiming to enhance the extraction process and make the production of oil and handling of fish byproducts more cost-effective. The wet reduction method, a solvent-free approach, was used for extraction, with yield optimization done via the Box-Behnken design. The resulting oil was evaluated for its oxidative quality and chemical characteristics. The optimal conditions for the wet rendering process were as follows: viscera to water ratio, 1:0.5 (w/v); temperature, 90℃; and time, 20 min, yielding 12.40 g/100 g of oil. The oil extracted under optimal wet rendering conditions had quality and oxidative stability comparable to solvent extraction and fewer secondary oxidation compounds. This oil had a higher PUFA content, specifically a 4:1 ratio of omega 6 to omega 3. Such oil, derived from catfish viscera, is suitable for the food industry due to its solvent-free extraction method.

7.
Exp Gerontol ; 194: 112501, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-38897017

RESUMEN

Diet significantly affects reproductive outcomes across species, yet the precise effects of macronutrient compositions beyond caloric intake on reproductive aging are understudied. Existing literature presents conflicting views on the fertility impacts of nutrient-rich versus nutrient-poor developmental diets, underscoring a notable research gap. This study addresses these gaps by examining effects of isocaloric diets with varied protein-to-carbohydrate ratios during both developmental and adult stages on reproductive aging of a large, outbred Drosophila melanogaster population (n = âˆ¼2100). Our results clearly demonstrate an age-dependent dietary impact on reproductive output, initially dominated by the developmental diet, then by a combination of developmental and adult diets in early to mid-life, and ultimately by the adult diet in later life. Importantly, we found that the effects of developmental and adult diets on reproductive output are independent, with no significant interaction. Further investigations into the mechanisms revealed that the effect of developmental diet on fecundity is regulated via ovarioles formation and vitellogenesis; while, the effect of adult diet on fecundity is mostly regulated only via vitellogenesis. These insights resolve disputes in the literature about dietary impacts on fertility and offer valuable perspectives for optimizing fertility strategies in improving public health and conservation efforts in this changing world.


Asunto(s)
Envejecimiento , Dieta , Drosophila melanogaster , Reproducción , Proteínas en la Dieta , Animales , Fertilidad , Vitelogénesis
8.
Cryobiology ; 116: 104931, 2024 Jul 10.
Artículo en Inglés | MEDLINE | ID: mdl-38909672

RESUMEN

Cryopreservation of goat spermatozoa is challenging due to several factors, including one of the most essential, i.e., oxidative stress. It is particularly essential in goat semen due to its scanty ejaculate volume and high sperm concentration. This leaves a narrow sperm-to-seminal plasma ratio owing to marginal antioxidant support; moreover, semen extension further dilutes the antioxidant level, leading to an imbalance of oxidant-antioxidant equilibrium. The present study aimed to evaluate the effect of quercetin on curtailing oxidative stress and its reflection on the post-thaw survivability and membrane integrity of goat spermatozoa. For this study, six bucks were selected. Six ejaculates from each buck totaling 36 ejaculates were collected, which were then split into five parts; furthermore, each part was added with a semen extender having a particular concentration of additive. Group C without quercetin and T1 containing Vitamin E at 3 mmol/mL were considered the control and positive control respectively, whereas T2, T3, and T4 contain 10, 20, and 30 µmol/mL of Quercetin respectively. The final sperm concentration of each group was kept at 200 × 106 spermatozoa/mL. All groups were subjected to equilibration at 4 °C for 4 h, then filled in French mini (0.25 mL) straws, followed by sealing and cryopreservation. Samples after 72 h of cryopreservation were subjected to evaluation of plasma membrane integrity and viability through staining, acrosomal integrity, and mitochondrial membrane activity through flow cytometry. Evaluation of sperm kinematics as well as the oxidant-antioxidant status of sperm (ROS and nitric oxide) and seminal plasma (SOD, CAT, GPx, FRAP, and lipid peroxidation through MDA estimation) were also carried out. Quercetin, when supplemented at 20 µmol/mL in buck semen extender, significantly (p < 0.01) improved cryopreserved sperm functions in terms of plasma membrane integrity, viability, acrosomal integrity, mitochondrial membrane activity, and sperm kinematics of buck semen. Similarly, Quercetin supplementation at 20 µmol/mL significantly reduced reactive oxygen and nitrogen species (RONS) in sperm and improved the antioxidant status of seminal plasma, which was indicated by reduced oxidative damage and improved the antioxidant status of buck semen. In conclusion, Quercetin at 20 µmol/mL reduced oxidative stress, improved semen antioxidant status, and improved sperm membranes integrity and kinematics.

9.
Indian J Dent Res ; 35(1): 111-113, 2024 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-38934761

RESUMEN

ABSTRACT: Gout is a metabolic disorder that leads to elevated serum uric acid levels and deposition of urate crystals in the joints. The disease is usually confined to the joint space and leads to pain and limitation of jaw opening. The case describes a 45-year-old female patient with a chief complaint of 'occasional pain in the left temporal muscle region'. The case disclosed a gout manifestation in the temporomandibular joint (TMJ) after physical and radiographic findings. Gout manifestation in the TMJ is an unusual presentation and a few reports in the English literature address the subject. Gout in the TMJ should be included as a differential diagnosis for joint disorders because of its rarity. A clinician may overlook gout involving the TMJ in the differential diagnosis of facial pain even when the patient has received a diagnosis of gout in other joints.


Asunto(s)
Artritis Gotosa , Trastornos de la Articulación Temporomandibular , Humanos , Femenino , Persona de Mediana Edad , Trastornos de la Articulación Temporomandibular/diagnóstico , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Artritis Gotosa/diagnóstico , Artritis Gotosa/complicaciones , Artritis Gotosa/diagnóstico por imagen , Diagnóstico Diferencial
10.
Cureus ; 16(5): e59576, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38826963

RESUMEN

Pancreatoduodenectomy is a complex surgical procedure involving three anastomoses. Anastomosis of the pancreatic stump with the gastrointestinal tract is associated with most complications described in the postoperative period. So, there have been multiple attempts to discover safe and sound steps for this particular anastomosis. Pancreaticogastrostomy involves anastomosis between the remaining pancreas and stomach. Since it was first performed, its surgical steps have been modified multiple times, but there is no gold standard method to perform it. In this paper, we describe the surgical steps of pancreaticogastrostomy in difficult pancreatic stumps in eight patients using two transpancreatic sutures, one purse string suture, and the incorporation of transpancreatic sutures in the third layer of the gastrojejunostomy anastomosis. Postoperative outcomes of this series have provided encouraging short-term results.

11.
Semin Plast Surg ; 38(1): 39-47, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38495060

RESUMEN

The financial burden of breast cancer treatment and reconstruction is a significant concern for patients. Patient desire for preoperative cost-of-care counseling while navigating the reconstructive process remains unknown. A cross-sectional survey of women from the Love Research Army was conducted. An electronic survey was distributed to women over 18 years of age and at least 1 year after postmastectomy breast reconstruction. Descriptive statistics and multivariable modeling were used to determine desire for and occurrence of cost-of-care discussions, and factors associated with preference for such discussions. Secondary outcomes included the association of financial toxicity with desire for cost discussions. Among 839 women who responded, 620 women (74.1%) did not speak to their plastic surgeon and 480 (57.4%) did not speak to a staff member regarding costs of breast reconstruction. Of the 550 women who reported it would have been helpful to discuss costs, 315 (57.3%) were not engaged in a financial conversation initiated by a health care provider. A greater proportion of women who reported financial toxicity, compared to those who did not, would have preferred to discuss costs with their plastic surgeon (65.2% vs. 43.5%, p < 0.001) or a staff member (75.5% vs. 59.3%, p < 0.001). Among women with financial toxicity, those who had some form of insurance (private, Medicaid, Medicare, "other") were significantly more likely to prefer a cost-of-care discussion ( p < 0.001, p = 0.02, p = 0.05, p = 0.01). Financial discussions about the potential costs of breast reconstruction seldom occurred in this national cohort. Given the reported preference and unmet need for financial discussions by a majority of women, better cost transparency and communication is needed.

12.
Artículo en Inglés | MEDLINE | ID: mdl-38482090

RESUMEN

In this report, we present a case of a 90-year-old female who exhibited a sudden onset of confusion and severe generalized weakness after receiving her second dose of mRNA SARS-CoV-19 vaccination 19 days prior to her admission in 2021. Her thyroid-stimulating hormone (TSH) levels were low, while her thyroxine (T4), erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels were elevated. Triiodothyronine (T3) level was not measured. Ultrasonography of the thyroid revealed multiple nodules with heterogeneous tissue, and a nuclear medicine thyroid uptake scan showed very low uptake. These findings indicated limited thyroid destruction in the form of subacute thyroiditis, likely triggered by the mRNA SARS-CoV-2 vaccine. This case illustrates an acute complication due to a novel vaccine. There are three key takeaways for physicians. First, there should be a discussion of the benefits and risks of Covid-19 vaccine. Second, patients who present with encephalopathy should have their thyroid function checked. Third, clinicians must be aware of the signs and symptoms of potentially life-threatening hyperthyroidism. Additional studies are needed to identify those patients at highest risk for Covid-19 vaccine complications.

13.
Sensors (Basel) ; 24(4)2024 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-38400371

RESUMEN

Electrolysis stands as a pivotal method for environmentally sustainable hydrogen production. However, the formation of gas bubbles during the electrolysis process poses significant challenges by impeding the electrochemical reactions, diminishing cell efficiency, and dramatically increasing energy consumption. Furthermore, the inherent difficulty in detecting these bubbles arises from the non-transparency of the wall of electrolysis cells. Additionally, these gas bubbles induce alterations in the conductivity of the electrolyte, leading to corresponding fluctuations in the magnetic flux density outside of the electrolysis cell, which can be measured by externally placed magnetic sensors. By solving the inverse problem of the Biot-Savart Law, we can estimate the conductivity distribution as well as the void fraction within the cell. In this work, we study different approaches to solve the inverse problem including Invertible Neural Networks (INNs) and Tikhonov regularization. Our experiments demonstrate that INNs are much more robust to solving the inverse problem than Tikhonov regularization when the level of noise in the magnetic flux density measurements is not known or changes over space and time.

14.
Biomacromolecules ; 25(3): 1481-1490, 2024 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-38343080

RESUMEN

Developing anticancer agents with negligible cytotoxicity against normal cells while mitigating multidrug resistance and metastasis is challenging. Previously reported cationic polymers have effectively eradicated cancers but are clinically unsuitable due to their limited selectivity. Herein, a series of poly(l-lysine)- and nicotinic acid-based polymers were synthesized using varying amounts of dodecylsuccinic anhydride. Zn-coordinating polymers concealed their cationic charge and enhanced selectivity. These Zn-bound polymers were highly effective against liver and colon cancer cells (HepG2 and Colon 26, respectively) and prevented cancer cell migration. They also displayed potent anticancer activity against drug-resistant cell lines (COR-L23/R): their cationic structure facilitated cancer cell membrane disruption. Compared to these polymers, doxorubicin was less selective and less efficacious against drug-resistant cell lines and was unable to prevent cell migration. These polymers are potential cancer treatment agents, offering a promising solution for mitigating drug resistance and tumor metastasis and representing a novel approach to designing cancer therapeutics.


Asunto(s)
Antineoplásicos , Neoplasias , Humanos , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Doxorrubicina/química , Neoplasias/tratamiento farmacológico , Neoplasias/patología , Polímeros/química , Zinc , Línea Celular Tumoral
15.
Ecol Evol ; 14(2): e10976, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38343564

RESUMEN

Food is fundamental for the survival of organisms, governing growth, maintenance, and reproduction through the provision of essential macronutrients. However, access to food with optimum macronutrient composition, which will maximize the evolutionary fitness of an organism, is not always guaranteed. This leads to dietary mismatches with potential impacts on organismal performance. To understand the consequences of such dietary mismatches, we examined the effects of isocaloric diets varying in macronutrient composition on eight key organismal traits spanning across the lifespan of a large outbred Drosophila melanogaster population (n ~ 2500). Our findings reveal that carbohydrate-reduced isocaloric diets correlates to accelerated pre-adult development and boosts reproductive output without impacting pre-adult viability and body size. Conversely, an elevated dietary carbohydrate content correlated to reduced lifespan in flies, evidenced by accelerated functional senescence including compromised locomotor activity and deteriorating gut integrity. Furthermore, transcriptomic analysis indicated a substantial difference in gene regulatory landscapes between flies subject to high-carbohydrate versus high-protein diet, with elevated protein levels indicating transcriptomes primed for reduced synthesis of fatty acids. Taken together, our study helps advance our understanding of the effect of macronutrient composition on life history traits and their interrelations, offering critical insights into potential adaptive strategies that organisms might adopt against the continual dietary imbalances prevalent in the rapidly evolving environment.

16.
Heliyon ; 10(3): e25330, 2024 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-38333841

RESUMEN

In the past decade, the demand and interest of consumers have expanded for using plant-based novel starch sources in different food and non-food processing. Therefore, millet-based value-added functional foods are acquired spare attention due to their excellent nutritional, medicinal, and therapeutic properties. Millet is mainly composed of starch (amylose and amylopectin), which is primary component of the millet grain and defines the quality of millet-based food products. Millet contains approximately 70 % starch of the total grain, which can be used as a, ingredient, thickening agent, binding agent, and stabilizer commercially due to its functional attributes. The physical, chemical, and enzymatic methods are used to extract starch from millet and other cereals. Numerous ways, such as non-thermal physical processes, including ultrasonication, HPP (High pressure processing) high-pressure, PEF (Pulsed electric field), and irradiation are used for modification of millet starch and improve functional properties compared to native starch. In the present review, different databases such as Scopus, Google Scholar, Research Gate, Science Direct, Web of Science, and PubMed were used to collect research articles, review articles, book chapters, reports, etc., for detailed study about millet starch, their extraction (wet milling process) and modification methods such as physical, chemical, biological. The impact of different modification approaches on the techno-functional properties of millet starch and their applications in different sectors have also been reviewed. The data and information created and aggregated in this study will give users the necessary knowledge to further utilize millet starch for value addition and new product development.

17.
Int J Biol Macromol ; 262(Pt 1): 129630, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38336319

RESUMEN

The current novel study aims was to development and characterization of gum based (guar gum: almond gum) composite formulations with or without addition of oregano essential oils to extend the shelf life of okra at ambient condition. In this study, the optimized composite of guar gum: almond gum (75:25 V/V) prepared with addition of different concentrations (0.05, 0.1 and 0.15 % (V/V) of oregano essential oils to study their physicochemical, rheological, antimicrobial and particle size & zeta potential distribution. In addition, the effects of prepared edible coatings on shelf-life of okra vegetables were also investigated by assessing their postharvest quality attributes at ambient (23 °C) storage up to 7 days storage. The results revealed, increasing concentration of essential oils in composite coating significantly increased in pH, TSS, particle size, antimicrobial (Apergillus. niger, Escherichia coli, Staphylococcus aureus) activity respectively. Furthermore, the increasing EOs improved viscosity (n) and stability of the coatings matrix. In addition, the applications of guar gum (0.25 %): almond gum (0.5 %) composite ratio (75,25) with oregano essential oils exhibited excellent properties and potential to maintain the postharvest characteristics of okra throughout the storage period. The results of this study revealed that the addition of higher concentration (0.15 %) of essential oils in composite formulation of 75 % guar gum +25 % almond gum (03) showed higher value of pH (5.45), antioxidant activity (20.87 %), particle size (899.1 nm), zeta potential (-8.6 mV), polydispersity index (50.6 %) and higher antimicrobial activity against E.coli (19 mm), S. aureus (29 mm) and A. niger (35 mm) as compared to other formulations. Therefore, the lower composite formulation (01) with lower concentration (0.05 %) of oregano essential oil was found most effective formulation to maintain the shelf life of okra for up to 4 days as compared to other treated and control okra samples at ambient temperature by retarded the weight loss (12.74 %), maintained higher firmness (0.998 N), lower respiration rate (484.32 ml Co2/kg/h) respectively on 7 days of storage. The microbial load in the okra samples treated with different guar gum: almond gum composite showed lower microbial load in terms of total plate count and yeast & mold counts as compared to control samples. Samples treated with O3 coating showed lowest TPC (0.1 × 108 cfu/g) and YMC (6.63 × 106 cfu/g) followed by O2 (0.48 × 108 cfu/g, 7.9 × 106 cfu/g) and O1 (0.78 × 108 cfu/g, 9.45 × 106 cfu/g) respectively on 6rd day of storage, overall results indicated that the application of composite coating with different concentrations of oregano essential oils were effective to maintained postharvest shelf life of okra up to 4 days at ambient condition.


Asunto(s)
Abelmoschus , Antiinfecciosos , Galactanos , Hibiscus , Mananos , Aceites Volátiles , Gomas de Plantas , Prunus dulcis , Conservación de Alimentos/métodos , Staphylococcus aureus , Antiinfecciosos/farmacología , Aceites Volátiles/farmacología , Esperanza de Vida
18.
Comput Biol Med ; 170: 108083, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38295479

RESUMEN

B-cell is an essential component of the immune system that plays a vital role in providing the immune response against any pathogenic infection by producing antibodies. Existing methods either predict linear or conformational B-cell epitopes in an antigen. In this study, a single method was developed for predicting both types (linear/conformational) of B-cell epitopes. The dataset used in this study contains 3875 B-cell epitopes and 3996 non-B-cell epitopes, where B-cell epitopes consist of both linear and conformational B-cell epitopes. Our primary analysis indicates that certain residues (like Asp, Glu, Lys, and Asn) are more prominent in B-cell epitopes. We developed machine-learning based methods using different types of sequence composition and achieved the highest AUROC of 0.80 using dipeptide composition. In addition, models were developed on selected features, but no further improvement was observed. Our similarity-based method implemented using BLAST shows a high probability of correct prediction with poor sensitivity. Finally, we developed a hybrid model that combines alignment-free (dipeptide based random forest model) and alignment-based (BLAST-based similarity) models. Our hybrid model attained a maximum AUROC of 0.83 with an MCC of 0.49 on the independent dataset. Our hybrid model performs better than existing methods on an independent dataset used in this study. All models were trained and tested on 80 % of the data using a cross-validation technique, and the final model was evaluated on 20 % of the data, called an independent or validation dataset. A webserver and standalone package named "CLBTope" has been developed for predicting, designing, and scanning B-cell epitopes in an antigen sequence available at (https://webs.iiitd.edu.in/raghava/clbtope/).


Asunto(s)
Antígenos , Epítopos de Linfocito B , Epítopos de Linfocito B/química , Secuencia de Aminoácidos , Antígenos/química , Conformación Molecular , Dipéptidos
19.
Int J Biol Macromol ; 254(Pt 3): 127234, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37797851

RESUMEN

In the current research, the pomegranate peel extract of varying concentrations (0.02, 0.04, 0.06, 0.08, and 0.1 g/mL) were incorporated into jackfruit seed starch (5 % w/w) based edible films and coatings for the evaluation their effects on the mechanical, physical, barrier and thermal properties. Furthermore, the effects of the optimized edible coating were investigated on the postharvest shelf life of white grapes at room storage (30 ± 5 °C, RH = 70 ± 5 %) conditions for up to 8 days. The obtained results showed a significant increment in thickness (p ≤ 0.05) with higher concentration (0.1 g/mL) of pomegranate peel extract (PPE), total phenolic content (959.33 ± 43.36 mg/100 g) and antioxidant activity (87.35 ± 1.64 %) of the prepared edible films but have negative impacts on the water vapor permeability (2.82 × 10-6 ± 6.48 × 10-7 g-1h-1pa-1) and oxygen permeability (1.62 × 10-14 ± 9.32 × 10-15 cm3·cm/cm2·s·cmHg), solubility (23.24 ± 3.21 %), and tensile strength (1.60 ± 0.43 MPa). The edible film enriched with 0.4 g/mL of PPE showed higher thermal stability in terms of glass transition temperature (98.2 ± 0.21 °C) and peak temperature (110.3 ± 0.35 °C). Additionally, the application of coating treatment significantly maintains the postharvest shelf life of white grapes throughout the storage period.


Asunto(s)
Artocarpus , Películas Comestibles , Granada (Fruta) , Vitis , Almidón , Semillas
20.
Data Brief ; 52: 109793, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38076469

RESUMEN

Boiling is used for the thermal management of high-energy-density devices and systems. However, sudden thermal runaway at boiling crisis often results in catastrophic failures. Machine learning is a promising tool for in-situ monitoring of boiling-based systems for preemptive control of boiling crisis. A carefully acquired and well-labeled dataset is a primary requirement for utilizing any data-driven learning framework to extract valuable descriptors. Here, we present a comprehensive dataset of boiling acoustics presented in our recent work [1]. We collect the audio files through meticulously controlled near-saturated pool boiling experiments under steady-state conditions. To this end, we connect a high-sensitivity hydrophone to a pre-amplifier and a data acquisition unit for accurate and reliable acquisition of acoustic signals. We organize the audio files into four categories as per the respective boiling regimes: background or natural convection (BKG, 2-5W/cm2), nucleate boiling (NB, 8-140W/cm2), excluding those at higher heat flux values preceding the onset of boiling crisis or the critical heat flux (Pre-CHF, ≈145W/cm2), and transition boiling (TB, uncontrolled). Each audio file label provides explicit information about the heat flux value and the experimental conditions. This dataset, consisting of 2056 files for BKG, 13367 files for NB, 399 files for Pre-CHF, and 460 files for TB, serves as the foundation for training and evaluating a deep learning strategy to predict boiling regimes. The dataset also includes acoustic emission data from transient pool boiling experiments conducted with varying heating strategies, heater surface, and boiling fluid modifications, creating a valuable dataset for developing robust data-driven models to predict boiling regimes. We also provide the associated MATLAB® codes used to process and classify these audio files.

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