Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros












Base de datos
Intervalo de año de publicación
1.
J Reprod Dev ; 70(3): 160-168, 2024 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-38494726

RESUMEN

Ovarian stimulation protocols are widely used to collect oocytes in assisted reproductive technologies (ARTs). Although the influence of ovarian stimulation on embryo quality has been described, this issue remains controversial. Here, we analyzed the influence of ovarian stimulation on developmental speed and chromosome segregation using live cell imaging. Female mice at the proestrus stage were separated by the appearance of the vagina as the non-stimulation (-) group, and other mice were administered pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) as the stimulation (+) groups. The cumulus-oocyte complexes from both groups were inseminated with sperm suspensions from the same male mice. Fertilization rates and developmental capacities were examined, and the developmental speed and frequency of chromosome segregation errors were measured by live-cell imaging using a Histone H2B-mCherry probe. The number of fertilized oocytes obtained was 1.4-fold more frequent in the stimulation (+) group. The developmental rate and chromosome stability did not differ between the groups. Image analysis showed that the mean speed of development in the stimulation (+) group was slightly higher than that in the non-stimulation (-) group. This increase in speed seemed to arise from the slight shortening of the 2- and 4-cell stages and third division lengths and consequent synchronization of cleavage timing in each embryo, not from the emergence of an extremely rapidly developing subpopulation of embryos. In conclusion, ovarian stimulation does not necessarily affect embryo quality but rather increases the chances of obtaining high-quality oocytes in mice.


Asunto(s)
Blastocisto , Desarrollo Embrionario , Oocitos , Inducción de la Ovulación , Animales , Femenino , Ratones , Desarrollo Embrionario/fisiología , Blastocisto/fisiología , Masculino , Oocitos/fisiología , Embarazo , Gonadotropinas Equinas/farmacología , Gonadotropina Coriónica/farmacología , Segregación Cromosómica , Fertilización In Vitro/métodos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...