Determination of contamination sources and geochemical reactions in groundwater of a mine area using Cu, Zn, and S-O isotopes.

To determine contamination sources and pathways, the use of multiple isotopes, including metal isotopes, can increase the reliability of environmental forensic techniques. This study differentiated contamination sources in groundwater of a mine area and elucidated geochemical processes using Cu, Zn, S-O, and O-H isotopes. Sulfate reduction and sulfide precipitation were elucidated using concentrations of dissolved sulfides, δ34SSO4, δ18OSO4, and δ66Zn. The overlying contaminated soil was possibly responsible for the contamination of groundwater at <5 mbgl, which was suggested by low δ65Cu values (0.419-1.120‰) reflecting those of soil (0.279-1.115‰). The existence of dissolved Cu as Cu(I) may prevent the increase in δ65Cu during leaching of contaminated soil in the sulfate-reducing environment. In contrast, the groundwater at >5 mbgl seemed to be highly affected by the contamination plume from the adit water, which was suggested by high SO42- concentrations (407-447 mg L-1) and δ65Cu (0.252-2.275‰) and δ66Zn (-0.105‰-0.362‰) values at a multilevel sampler approaching those of the adit seepages. Additionally, the O-H isotopic ratios were distinguished between <5 mbgl and >5 mbgl. Using δ65Cu and δ66Zn to support the determination of groundwater contamination sources may be encouraged, particularly where the isotopic signatures are distinct for each source.

Determination of contamination sources and geochemical behaviors of metals in soil of a mine area using Cu, Pb, Zn, and S isotopes and positive matrix factorization.

The use of multiple isotopic ratios and statistical methods can substantially increase the reliability and precision of determining contamination sources and pathways. In this study, contamination sources were differentiated in three subareas in one mine area and geochemical processes were investigated using Cu, Pb, Zn, and S isotopes and positive matrix factorization (PMF). Soil samples downstream of the adit seepages exhibited distinctly higher δ65Cu values than those from other areas. δ65Cu in adit seepages increased substantially from ore sulfides owing to large isotopic fractionation during oxidative dissolution. Although δ65Cu decreased during sulfide precipitation in seepage-contaminated soil, the discrimination of δ65Cu was still valid. Therefore, δ65Cu is particularly useful for differentiating between contamination by sulfides (tailings) and water (adit seepages). Moreover, sulfide precipitation following sulfate reduction was verified by the decreased δ66Zn and δ34S in the soil. In addition, the plot of 208Pb/206Pb versus Pb-1 distinguished contamination sources. Furthermore, PMF analysis confirmed the determination of sources and differentiated between contamination by As- and Cu-enriched tailings. The effect of Cu-enriched tailings further downstream suggested that the lower specific gravity of chalcopyrite compared to that of arsenopyrite affected the distribution of soil contamination.

Pilot-scale subcritical solvent extraction of curcuminoids from Curcuma long L.

Curcuminoids consisted curcumin, demethoxycurcumin and bisdemethoxycurcumin, were extracted from turmeric using subcritical solvent by varying conditions of temperature (110-150 °C), time (1-10 min), pressure (5-100 atm), solid-to-solvent ratio, and mixing ratio of solvent. Preliminary lab-scale experiments were conducted to determine the optimum extraction temperature and mixing ratio of water and ethanol for the pilot-scale extraction. The maximum yield of curcuminoids in the pilot-scale system was 13.58% (curcumin 4.94%, demethoxycurcumin 4.73%, and bisdemethoxycurcumin 3.91% in dried extracts) at 135 °C/5 min with water/ethanol mixture (50:50, v/v) as a solvent. On the other hand, the extraction yields of curcuminoids were obtained as 10.49%, 13.71% and 13.96% using the 50%, 95% and 100% ethanol, respectively, at the atmospheric condition (60 °C/120 min). Overall results showed that the subcritical solvent extraction is much faster and efficient extraction method considering extracted curcuminoids contents and has a potential to develop a commercial process for the extraction of curcuminoids.