RESUMEN
- In Ghana, mining of minerals at small-scale and large-scale is widespread across many districts, leading to significant heavy metal pollution in the environment. In this study, the concentrations of iron (Fe), manganese (Mn), arsenic (As), and mercury (Hg) in the different drinking water samples collected from households, institutions, water points and surface water in two mining districts namely the Wassa East and Asutifi North were analyzed. The water types collected included boreholes, wells, piped water into yards, public standpipes, rainwater, sachet water, and surface water. The results indicated that the levels of Fe and Mn were higher than As and Hg in all the drinking water samples. The levels of As and Hg in drinking water from households and institutions were higher in the Wassa East district compared to the Asutifi North district. However, the metal levels at water points were similar in both districts. In surface water, Fe levels were higher in the Wassa East district compared to the Asutifi North district with median values of 1243 µg/L and 860 µg/L for the Wassa East and Asutifi North districts, respectively. In contrast, the Mn levels were higher in the Asutifi North district than the Wassa East district with median values of 9.5 µg/L and 90 µg/L for Wassa East and Asutifi North districts, respectively. All the metals (Mn, As, and Hg) studied except Fe were within the recommended WHO level. The Heavy Metal Pollution Index (HPI) values for the different water types in households, institutions and water points were all below the critical limit of 100. The Water Quality Index (WQI) indicates that the boreholes, piped water into yards, and public standpipes in both districts were classified as excellent or good, making them suitable for drinking. However, the wells and surface water in both districts were classified as very poor and unfit for drinking, respectively.
RESUMEN
BACKGROUND: A significant decrease in malaria morbidity and mortality has been attained using long-lasting insecticide-treated nets and indoor residual spraying. Selective pressure from these control methods influences changes in vector bionomics and behavioural pattern. There is a need to understand how insecticide resistance drives behavioural changes within vector species. This study aimed to determine the spatio-temporal dynamics and biting behaviour of malaria vectors in different ecological zones in Ghana in an era of high insecticide use for public health vector control. METHODS: Adult mosquitoes were collected during the dry and rainy seasons in 2017 and 2018 from five study sites in Ghana in different ecological zones. Indoor- and outdoor-biting mosquitoes were collected per hour from 18:00 to 06:00 h employing the human landing catch (HLC) technique. Morphological and molecular species identifications of vectors were done using identification keys and PCR respectively. Genotyping of insecticide-resistant markers was done using the TaqMan SNP genotyping probe-based assays. Detection of Plasmodium falciparum sporozoites was determined using PCR. RESULTS: A total of 50,322 mosquitoes belonging to four different genera were collected from all the study sites during the sampling seasons in 2017 and 2018. Among the Anophelines were Anopheles gambiae s.l. 93.2%, (31,055/33,334), An. funestus 2.1%, (690/33,334), An. pharoensis 4.6%, (1545/33,334), and An. rufipes 0.1% (44/33,334). Overall, 76.4%, (25,468/33,334) of Anopheles mosquitoes were collected in the rainy season and 23.6%, (7866/33,334) in the dry season. There was a significant difference (Z = 2.410; P = 0.0160) between indoor-biting (51.1%; 15,866/31,055) and outdoor-biting An. gambiae s.l. (48.9%; 15,189/31,055). The frequency of the Vgsc-1014F mutation was slightly higher in indoor-biting mosquitoes (54.9%) than outdoors (45.1%). Overall, 44 pools of samples were positive for P. falciparum CSP giving an overall sporozoite rate of 0.1%. CONCLUSION: Anopheles gambiae s.l. were more abundant indoors across all ecological zones of Ghana. The frequency of G119S was higher indoors than outdoors from all the study sites, but with higher sporozoite rates in outdoor mosquitoes in Dodowa and Kpalsogu. There is, therefore, an urgent need for a supplementary malaria control intervention to control outdoor-biting mosquitoes.
