Nondestructive circadian profiling of starch content in fresh intact Arabidopsis leaf with two-photon fluorescence and second-harmonic generation imaging.

Plant chloroplasts conduct photosynthesis to convert solar energy into sugars for the carbon source essential for cell living and growth during the day. One fraction of photosynthetic products is stored in chloroplasts by forming starch granules to continue the provision of carbon energy during the night. Currently, profiling the starch temporal pattern requires either: (i) sacrificing the leaves, or (ii) generating transgenic plants at the risk of changing the metabolisms by incorporating a genetically modified granule-bound starch synthase (GBSS). In this paper, we demonstrated a nondestructive method using two-photon fluorescence (TPF) and second-harmonic generation (SHG) imaging to quantify starch granules within chloroplasts of fresh intact leaves across a day-night cycle. We did so using two Arabidopsis lines having normal and excess starch contents: wild-type (Columbia-0) and starch excess 1 (sex1). The starch granules were visualized by SHG imaging, while the chloroplasts in mesophyll cells were visualized by TPF imaging. Our results provided micron scale spatial resolution of starch distribution within leaves and showed starch circadian patterns consistent with those profiled by enzymatic assays in previous studies. We demonstrated that TPF-SHG imaging is a potential tool for revealing the real-time heterogeneity of starch circadian rhythm in leaf cells, without the need for destructive sample preparation.

Using Exciton/Trion Dynamics to Spatially Monitor the Catalytic Activities of MoS2 during the Hydrogen Evolution Reaction.

The adsorption and desorption of electrolyte ions strongly modulates the carrier density or carrier type on the surface of monolayer-MoS2 catalyst during the hydrogen evolution reaction (HER). The buildup of electrolyte ions onto the surface of monolayer MoS2 during the HER may also result in the formation of excitons and trions, similar to those observed in gate-controlled field-effect transistor devices. Using the distinct carrier relaxation dynamics of excitons and trions of monolayer MoS2 as sensitive descriptors, an in situ microcell-based scanning time-resolved liquid cell microscope is set up to simultaneously measure the bias-dependent exciton/trion dynamics and spatially map the catalytic activity of monolayer MoS2 during the HER. This operando probing technique used to monitor the interplay between exciton/trion dynamics and electrocatalytic activity for two-dimensional transition metal dichalcogenides provides an excellent platform to investigate the local carrier behaviors at the atomic layer/liquid electrolyte interfaces during electrocatalytic reaction.

Photosensitivity of gallium-doped silica core fiber to 193 nm ArF excimer laser.

Grating inscription in a Ga-doped silica core fiber (~5 wt. % Ga) has been demonstrated using ArF (193 nm) and KrF (248 nm) excimer lasers. In a comparative study with germanosilicate fiber with similar Ge concentration, a Ga-doped silica core fiber shows greater photosensitivity to an ArF excimer laser due to the higher absorbance in the region of 190-195 nm. In addition, the photosensitivity of a Ga-doped silica core fiber has been greatly enhanced with hydrogenation. Ga-doped fibers are potential photosensitive fibers for fiber Bragg grating production with an ArF excimer laser.

Measurement of grating visibility of a fiber Bragg grating based on bent-spectral analysis.

In this study, a technique for measuring the grating visibility of the fiber Bragg grating (FBG) based on bent-spectral analysis is proposed. From varying ac and dc coupling coefficients at different bending radii, the grating visibility is estimated with the aid of a simple mathematical model. The investigation begins with the estimation of the grating visibility from the transmission spectra of the FBG during the inscription process. After that, the FBGs are subjected to a bending test with reducing radii, and again the transmission spectra are recorded. It is shown that the estimated grating visibility is in agreement with the result determined from the earlier inscription process.

Observation of grating regeneration by direct CO(2) laser annealing.

In this work, we have demonstrated for the first time grating regeneration in hydrogenated fibers by direct CO(2) laser annealing. During the annealing process, the center wavelength redshifts as the intensity of the focused CO(2) laser on the grating is elevated. The reflectivity of the grating begins to decay as the temperature induced in the grating approaches the erasure temperature. The grating is completely erased and regenerated afterwards. The observed spectral results have provided the proof of occurrence of dehydroxylation and stress relaxation in the fiber core during the annealing process. Regenerated gratings with low loss, good temperature sensitivities and sustainability have been successfully developed by this technique.

Thermal stress modification in regenerated fiber Bragg grating via manipulation of glass transition temperature based on CO2-laser annealing.

In this work, we have demonstrated thermal stress relaxation in regenerated fiber Bragg gratings (RFBGs) by using direct CO2-laser annealing technique. After the isothermal annealing and slow cooling process, the Bragg wavelength of the RFBG has been red-shifted. This modification is reversible by re-annealing and rapid cooling. It is repeatable with different cooling process in the subsequent annealing treatments. This phenomenon can be attributed to the thermal stress modification in the fiber core by means of manipulation of glass transition temperature with different cooling rates. This finding in this investigation is important for accurate temperature measurement of RFBG in dynamic environment.

Chronology of Fabry-Perot interferometer fiber-optic sensors and their applications: a review.

Optical fibers have been involved in the area of sensing applications for more than four decades. Moreover, interferometric optical fiber sensors have attracted broad interest for their prospective applications in sensing temperature, refractive index, strain measurement, pressure, acoustic wave, vibration, magnetic field, and voltage. During this time, numerous types of interferometers have been developed such as Fabry-Perot, Michelson, Mach-Zehnder, Sagnac Fiber, and Common-path interferometers. Fabry-Perot interferometer (FPI) fiber-optic sensors have been extensively investigated for their exceedingly effective, simple fabrication as well as low cost aspects. In this study, a wide variety of FPI sensors are reviewed in terms of fabrication methods, principle of operation and their sensing applications. The chronology of the development of FPI sensors and their implementation in various applications are discussed.

Spectral analysis of bent fiber Bragg gratings: theory and experiment.

This Letter presents a simple mathematical model developed from coupled-mode theory to describe the relationship between Bragg transmission loss (BTL), grating length, coupling coefficients, and bending loss in a bent fiber Bragg grating. In our investigation, the finding indicates that the decrement of BTL can be attributed to the increasing bending loss and degradation of both dc and ac coupling coefficients as the bending radius decreases. Besides, the center wavelength shifts as a result of coupling coefficients degradation. The validity of the proposed model is supported by experimental result.