Localization of protein kinase C isoforms in the optic pathway of mouse embryos and their role in axon routing at the optic chiasm.

Protein kinase C (PKC) plays a key role in many receptor-mediated signaling pathways that regulate cell growth and development. However, its roles in guiding axon growth and guidance in developing neural pathways are largely unknown. To investigate possible functions of PKC in the growth and guidance of axons in the optic chiasm, we first determined the localization of major PKC isoforms in the retinofugal pathway of mouse embryos, at the stage when axons navigate through the midline. Results showed that PKC was expressed in isoform specific patterns in the pathway. PKC-α immunoreactivity was detected in the chiasm and the optic tract. PKC-ßΙΙ was strong in the optic stalk but was attenuated on axons in the diencephalon. Immunostaining for PKC-ε showed a colocalization in the chiasmatic neurons that express a surface antigen stage specific embryonic antigen-1 (SSEA-1). These chiasmatic neurons straddled the midline of the optic chiasm, and have been shown in earlier studies a role in regulation of axon growth and guidance. Expression levels of PKC-ßΙ, -δ and -γ were barely detectable in the pathway. Blocking of PKC signaling with Ro-32-0432, an inhibitor specific for PKC-α and -ß at nanomolar concentration, produced a dramatic reduction of ipsilateral axons from both nasal retina and temporal crescent. We conclude from these studies that PKC-α and -ßΙΙ are the predominant forms in the developing optic pathway, whereas PKC-ε is the major form in the chiasmatic neurons. Furthermore, PKC-α and -ßΙΙ are likely involved in signaling pathways triggered by inhibitory molecules at the midline that guide optic axons to the uncrossed pathway.

Role of protein kinase C in selective inhibition of mouse retinal neurites during contacts with chondroitin sulfates.

Chondroitin sulfate proteoglycans elicit a selective inhibition to neurite growth from ventrotemporal (VT) but not dorsonasal (DN) retina, potentiating the bilateral routing of axons in the mouse optic chiasm. We examined whether this selective response is mediated by a difference in protein kinase C (PKC) expression. Effects of suppressing PKC activity in explant preparations of embryonic day 14 retinae with inhibitor Gö6976 or Ro-32-0432 abolished the chondroitin sulfate inhibition to the VT neurites but had no effect to the DN neurites. Whether these responses rely on a difference in expression of PKC in the growth cones was examined using antibodies against six isozymes of PKC. Among these the alpha, betaI and epsilon isozymes were expressed prominently in the retinal growth cones; whilst the betaII, delta and gamma isozymes were barely detected. Moreover, while the alpha and epsilon isozymes were abundant in the filopodial and lamellipodial processes, the betaI isozyme was restricted largely in the core region of the growth cones. Despite these subtype specific localization, there was no significant difference in expression of any of these PKC isozymes between growth cones from VT and DN retina, indicating that the selective response to chondroitin sulfates is not likely generated by a regulation of PKC expression, but by expression of surface molecules that interact with chondroitin sulfate proteoglycans.