RESUMEN
We compared complete genome sequences of two strains of an avian influenza A (H5N6) virus isolated from a patient in Anhui Province with those of other strains from GenBank and Global initiative on sharing all influenza data (GISAID). The HA gene of the isolated virus shared homology with that of A/chicken/Zhejiang/727155/2014 (H5N6) at the level of similarity of 98%. The six internal genes of the Anhui strains were close to those of H9N2 viruses from Zhejiang, Shandong, and Guangdong provinces, with a similarity of 99%. In addition, the similarity between the internal antigens (NP and MP) of the isolated H5N6 virus and H7N9 and H10N8 viruses was 99%. Based on the data of phylogenetic analysis, the H5N6 influenza virus isolated in Anhui Province belonged to clade 2.3.4.4. The virus was shown to have molecular characteristics of highly pathogenic avian influenza viruses, including eight glycosylation sites and an amino acid sequence of the HA protein cleavage site, PLRERRRKKR/GLF, containing multiple basic amino acids. Additionally, the stalk domain of the NA protein was found to have a deletion in NA stalk region (11 amino acids in N6, positions 58-68). Our study demonstrated that the H5N6 virus from Anhui Province represented a triple-reassortant virus and could be highly pathogenic to humans. The prevalence of this virus should be closely monitored.
Asunto(s)
Virus de la Influenza A/genética , Gripe Aviar/virología , Gripe Humana/virología , Virus Reordenados/genética , Anciano , Animales , Pollos , China/epidemiología , Femenino , Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Humanos , Virus de la Influenza A/clasificación , Virus de la Influenza A/aislamiento & purificación , Neuraminidasa/genética , Virus Reordenados/clasificación , Virus Reordenados/aislamiento & purificación , Análisis de Secuencia de ADN , Proteínas Virales/genética , Virulencia , Factores de Virulencia/genéticaRESUMEN
We isolated Rickettsia japonica from a febrile patient in Lu'an City, China, in 2013. Subsequently, we found an R. japonica seroprevalence of 54.8% (494/902) in the rural population of Anhui Province and an R. japonica prevalence in Haemaphysalis longicornis ticks of 0.5% (5/935). R. japonica and its tick vector exist in China.
Asunto(s)
Vectores Arácnidos/microbiología , Ixodidae/microbiología , Rickettsia/inmunología , Rickettsiosis Exantemáticas/microbiología , Animales , China/epidemiología , Humanos , Masculino , Persona de Mediana Edad , Filogenia , Rickettsia/genética , Rickettsia/aislamiento & purificación , Estudios Seroepidemiológicos , Rickettsiosis Exantemáticas/diagnóstico , Rickettsiosis Exantemáticas/epidemiología , Mordeduras de GarrapatasRESUMEN
We conducted an investigation of Orientia tsutsugamushi infection among rodents in non-endemic areas in Anhui Province. Fifty-six (56) rodents including 44 Apodemus agrarius and 12 Niviventer niviventer were trapped and captured in autumn in the fields of three counties in Anhui Province. DNA samples were amplified and sequenced for the 56kDa protein gene of Orientia tsutsugamushi. The overall infection rate in the rodents was 23.2%(13/56). The rate of detection of O. tsutsugamushi in Apodemus agrarius and Niviventer niviventer were 27.3% and 8.3% respectively. Moreover, we identified two genotypes (Karp and Gilliam strains) of Orientia tsutsugamushi in rodents. Our study demonstrated that Apodemus agrarius is the main host for O. tsutsugamushi pathogen and this is the first report of Karp and Gilliam strains in these non-endemic areas in Anhui Province.
Asunto(s)
Enfermedades de los Animales/epidemiología , Enfermedades de los Animales/microbiología , Orientia tsutsugamushi , Roedores/virología , Tifus por Ácaros/veterinaria , Animales , China/epidemiología , Genes Bacterianos , Orientia tsutsugamushi/clasificación , Orientia tsutsugamushi/genética , Filogenia , Análisis de Secuencia de ADNRESUMEN
OBJECTIVE: Shift work has been implicated in cardiovascular disease (CVD), a major cause of death globally. The aim of this study was to evaluate the risk of developing CVD in shift work. DESIGN: A cross-sectional study involving secondary analysis of shift and non-shift work from an industry in Ghana. PARTICIPANTS: Two hundred (113 shift and 87 non-shift) consecutive workers who consented were recruited into the study. A structured questionnaire was administered to deduce information on participant's age, alcohol consumption pattern, smoking habits, history of diabetes, stroke and hypertension. RESULTS: Shift workers were found to be associated with higher body mass index (26.9 ± 4.6 vs 25.2 ± 3.3, p = 0.013); fasting blood glucose (5.9 ± 1.8 vs 5.3 ± 0.8, p ≤ 0.0001); glycated haemoglobin (4.9 ± 0.9 vs 4.2 ± 0.8, p ≤ 0.0001); high sensitivity C-reactive protein (2.5 ± 1.1 vs 1.8 ± 1.1, p < 0.0001); total cholesterol (5.9 ± 1.3 vs 5.2 ± 1.7, p = 0.002); triglycerides (1.3 ± 0.8 vs 1.1 ± 0.6, p = 0.015) and LDL cholesterol (3.6 ± 0.9 vs 3.2 ± 1.3, p = 0.04) than controls. Shift work however, had no associations with HDL-cholesterol. CONCLUSION: It can be concluded that shift work is associated with risk factors of CVD.
