Effect of Ammonia and Indole-3-acetic Acid Producing Endophytic Klebsiella pneumoniae YNA12 as a Bio-Herbicide for Weed Inhibition: Special Reference with Evening Primroses.

Information on the use of endophytic bacteria as a bio-herbicide for the management of weed control in agricultural fields is limited. The current study aimed to isolate endophytic bacteria from evening primroses and to screen them for their bio-herbicidal activity. Two isolated endophytic bacteria (Pantoea dispersa YNA11 and Klebsiella pneumoniae YNA12) were initially screened for citrate utilization and for indole-3-acetic acid (IAA) and catalase production. The preliminary biochemical assessment showed YNA12 as a positive strain. Ammonia, catalase, and IAA in its culture filtrate were quantified. Gas Chromatography/Mass Spectroscopy- Selective Ion Monitoring (GC/MS-SIM) analysis revealed the production of IAA by YNA12 in a time-dependent manner. YNA12 also exhibited significant ammonia-producing potential and catalase activity against hydrogen peroxide. The YNA12 culture filtrate significantly inhibited the germination rate of evening primrose seeds, resulting in a marked reduction in seedling length and biomass compared with those of the control seeds. Moreover, the culture filtrate of YNA12 significantly accelerated the endogenous abscisic acid (ABA) production and catalase activity of evening primrose seedlings. Macronutrient regulation was adversely affected in the seedlings exposed to the culture filtrate of YNA12, leading to inhibition of seed germination. The current results suggest that endophytic YNA12 may be used as a potent bio-herbicidal agent for controlling weed growth and development.

Developing Fall-impact Protection Pad with 3D Mesh Curved Surface Structure using 3D Printing Technology.

In this study, we present the development of fall-impact protection pads for elderly people using three-dimensional (3D) printing technology. To develop fall-impact protection clothing, it is important to maintain the functionality of the protection pad while ensuring that its effectiveness and appearance remain optimal in the process of inserting it. Therefore, this study explores the benefit of exploiting 3D scan data of the human body using 3D printing technology to develop a fall-impact protection pad that is highly suited to the human body shape. The purpose of this study was to present a 3D modeling process for creating curved protective pads comprising a hexagonal mesh with a spacer fabric structure and to verify the impact protection performance by printing curved pads. To this end, we set up a section that includes pads in the 3D human body scan data and extracted body surface information to be applied in the generation of the pad surface. The sheet-shaped hexagonal mesh structure was cut and separated according to the pad outline, and then deformed according to the curved surface of the human body. The pads were printed, and their protection performance was evaluated; a 79.2-81.8% reduction in impact force was observed compared to similar cases in which the pads were not used.

Development and Evaluation of Fall Impact Protection Pads Using Additive Manufacturing.

This paper presents the development and evaluation of fall-impact protection pants for elderly women using additive manufacturing. The protective pants were designed incorporating a protective pad in the hip area to reduce the impact of falls on the human body. The protective pad is a 3D mesh structure with a curved surface to fit the human body. Pads printed with flexible thermoplastic polyurethane were combined with foam to create the final pad. The impact-absorbing performance of the pad was verified through physical impact experiments. When dropping a bowling ball onto the protective pad from heights of 15, 20, and 25 cm, the protective pad was found to reduce the impact force by more than 82% in all cases. The impact force was less than the average fracture threshold of 3472 N. A subject group and an expert group evaluated the appearance, pad characteristics, motion functionality, and the wearability of the protection pants. Despite the insertion of a pad, the pants appeared natural and had a good fit. The pads were evaluated as being well-designed in terms of their position, shape, area, thickness, weight, flexibility, ease of insertion, and ease of use. Users were comfortable performing various motions when wearing the designed protective clothing. Therefore, this work can be considered to have developed protective clothing that provides satisfactory impact-protection performance and comfort thereby advancing the possibility of applying additive manufacturing to the creation of functional garments.

Axis I comorbidity and psychopathologic correlates of autodestructive syndromes.

OBJECTIVE: Nonsuicidal deliberate self-harm and factitious disorders have been proposed as subtypes within the autodestructive behavior spectrum, basically differing in the issue of concealment. Aims are to determine Axis I diagnoses and psychopathologic correlates of open self-harmers and patients diagnosed with factitious disorders. METHODS: One hundred ninety-four psychosomatic medicine inpatients participated. Assessment included the structured World Health Organization Composite International Diagnostic Interview (computerized version) and self-report questionnaires for anxiety, depression, perceived stress, and personal coping resources. RESULTS: Thirty-seven patients identified with self-destructive behavior were matched with 37 patients without such behavior. Overt self-harmers (n = 18) were more frequently diagnosed with anxiety, depressive, substance abuse/dependence, or eating disorders and reported more stress than factitious disorder patients (n = 19) or those without self-destructive behavior. Patients with factitious disorder exhibited lower Axis I comorbidity and less psychopathology than patients without self-harm behavior. CONCLUSIONS: Regarding psychopathologic assessment, contrary to open self-harmers, factitious disorder patients appear strikingly inconspicuous.

Risk factors and correlates of deliberate self-harm behavior: a systematic review.

OBJECTIVE: Deliberate self-harm behavior--without suicidal intent--is a serious health problem and may be studied as a clinical phenomenon in its own right. Empirical studies of sociodemographic and psychological correlates and risk factors are systematically reviewed. METHODS: We searched Medline, PsycINFO, PSYNDEX (German psychological literature), and reference lists. We targeted self-induced bodily harm without conscious suicidal intent. Studies on suicidal behavior or self-poisoning were only included if they also assessed nonsuicidal self-harm. RESULTS: Fifty-nine original studies met the criteria. Deliberate self-harm may occur at all ages, yet adolescents and young adults are at a higher risk. Evidence on gender is complex. Only 5 studies realize a prospective design (6 months to 10 years) and test predictors. The majority use cross-sectional and retrospective methods. No longitudinal study (separately) examines new incidence. Evidence of correlates encompasses distal/proximal, person/environment, and state/trait factors. Many studies report associations between current self-harm behavior and a history of childhood sexual abuse. Adolescent and adult self-harmers experience more frequent and more negative emotions, such as anxiety, depression, and aggressiveness, than persons who do not self-harm. Two studies yield specific interactions between childhood trauma and current traits and states such as low emotional expressivity, low self-esteem, and dissociation with respect to a vulnerability to self-harm. CONCLUSION: Evidence of distal, biographical stressors is fairly strong. Proximal stressors have rarely been investigated; protective factors, hardly at all. Despite many findings of correlates, the data do not yet justify terming them risk factors. Longitudinal studies are needed.

[The effects of meridian massage on menopausal symptoms and Shin-Hur in middle-aged menopausal women].

PURPOSE: The purpose of this study was to identify the effects of meridian massage on menopausal symptoms and Shin-Hur in middle-aged menopausal women. METHOD: The research design was a nonequivalent control group pre-post experimental design. The subjects of the study were middle-aged women who had had no menstruation in the last 12 months after the last menstrual bleeding. Cards of invitation on bulletin boards of several apartments were placed to recruit the subjects. The cards of invitation included: purpose of the study, eligibility criteria, method and period. Eighteen women in the experimental group and 16 women in the control group were conveniently assigned, respectively. The experimental group received 20 min meridian massage 3 times per week for 4 weeks. The menopausal symptoms and Shin-Hur were measured and compared between the two groups before and after the intervention. Data were analyzed with the SPSS program by Fisher's exact test, Wilcoxon Sign Rank test, Mann Whitney U-test and Spearman's rank correlation. RESULT: The experimental group showed a significant decrease of menopausal symptoms (U=77.00, p=.020) and Shin-Hur (U=76.00, p=.017). There was a significantly positive correlation between menopausal symptoms and Shin-Hur (r=.497, p=.003). CONCLUSION: Meridian massage was effective in improving menopausal symptoms and Shin-Hur in middle-aged menopausal women. Thus it can be useful as a nursing intervention for menopausal women.