RESUMEN
Conventional photosensitizers (PSs) used in photodynamic therapy (PDT) have shown preliminary success; however, they are often associated with several limitations including potential dark toxicity in healthy tissues, limited efficacy under acidic and hypoxic conditions, suboptimal fluorescence imaging capabilities, and nonspecific targeting during treatment. In response to these challenges, we developed a heavy-atom-free PS, denoted as Cz-SB, by incorporating ethyl carbazole into a thiophene-fused BODIPY core. A comprehensive investigation into the photophysical properties of Cz-SB was conducted through a synergistic approach involving experimental and computational investigations. The enhancement of intersystem crossing (kISC) and fluorescence emission (kfl) rate constants was achieved through a donor-acceptor pair-mediated charge transfer mechanism. Consequently, Cz-SB demonstrated remarkable efficiency in generating reactive oxygen species (ROS) under acidic and low-oxygen conditions, making it particularly effective for hypoxic cancer PDT. Furthermore, Cz-SB exhibited good biocompatibility, fluorescence imaging capabilities, and a high degree of localization within the mitochondria of living cells. We posit that Cz-SB holds substantial prospects as a versatile PS with innovative molecular design, representing a potential "one-for-all" solution in the realm of cancer phototheranostics.
Asunto(s)
Mitocondrias , Imagen Óptica , Fotoquimioterapia , Fármacos Fotosensibilizantes , Especies Reactivas de Oxígeno , Fármacos Fotosensibilizantes/química , Fármacos Fotosensibilizantes/farmacología , Humanos , Mitocondrias/metabolismo , Mitocondrias/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Boro/química , Compuestos de Boro/farmacología , Carbazoles/química , Carbazoles/farmacología , Células HeLa , Tiofenos/química , Tiofenos/farmacología , Línea Celular TumoralRESUMEN
Magnesium oxide (MgO) nanoparticles are commonly used to enhance the reactivity and performance of devices and systems in various applications, primarily due to the heat-resistance, binding, and alkaline properties of MgO. However, most of the methods used to synthesize MgO nanoparticles suffer from nonuniform particle size distributions that make it difficult to manufacture stable particles. In this study, uniform magnesium oxide (MgO) nanoparticles were developed for TiO2 photoelectrodes of dye-sensitized solar cells (DSSCs) to enhance their interfacial resistances. The uniform MgO nanoparticles were synthesized from MgO 93% using a poly(acrylic acid) template-assisted method. The particle size and crystalline structure of MgO nanoparticles were characterized by NANOPHOX particle size analysis, transmission electron microscopy, and X-ray diffraction. Multilayered TiO2 photoelectrodes containing interlayers of MgO nanoparticles were fabricated as photoelectrodes for DSSC devices, and their photovoltaic performances were investigated. When the MgO interlayer was introduced into the multilayered TiO2 photoelectrode, it not only increased the photocurrent value of the DSSC device but also improved its power conversion efficiency. The DSSC device containing the MgO interlayer and the scattering layer exhibited an open-circuit voltage of 0.74 V, a short-circuit current density of 14.60 mA/cm2, and a fill factor of 0.64 under a photointensity of 100 mW/cm2 at AM 1.5, resulting in an overall solar energy conversion efficiency of 6.94%. The application of an MgO interlayer in a DSSC device exhibited improved conductivity, charge transfer ability, and excellent device performance.
RESUMEN
To date, a large number of photosensitizers (PS) have introduced heavy atoms to improve the ISC process and 1O2 generation. However, they often show low efficiency in hypoxic conditions, aggregate states, and turn-off PDT in the dark. Besides that, the toxicity of heavy metals is also concerned. Therefore, we developed lysosome-targeted heavy-metal-free PS (3S and 4S) based on thionated naphthalimide for hypoxic cancer photodynamic therapy (PDT), not only under white light but also in the dark via thermal-induced 1O2 generation. AIEgen (3O and 4O) were prepared for studying the PDT action of PSs (3S and 4S) in lysosome and aggregate state. We also examined the photophysical properties of AIEgen (3O and 4O) and PS (3S and 4S) by UV-vis absorption, fluorescent emission spectra, and theoretical calculations.
Asunto(s)
Neoplasias , Fotoquimioterapia , Humanos , Luz , Lisosomas , Neoplasias/tratamiento farmacológico , Fármacos Fotosensibilizantes/uso terapéuticoRESUMEN
We prepared the monomer PCDA-HP composed of 5-hydroxy-N 1,N 3-bis(pyridin-2-ylmethyl)isophthalamide (HP) as a cadmium ion tweezer and then polymerized them to form a polydiacetylene (PDA)-based sensor (PDA-HP), which displayed selective and sensitive colorimetric and fluorometric change upon addition of a cadmium ion (Cd2+) at both pH 7.4 and 6.8. The PDA-HP polymer was highly selective for Cd2+ over other metal ions with colorimetric change. In addition, the PDA-HP chemosensor also showed a red fluorescence change in the presence of Cd2+ at both pH 7.4 and 6.8. Naked-eye detection of Cd2+ was accomplished in an aqueous solution through a PDA-based sensor system. Finally, the lowest energy structure of an HP chelator was obtained by the crystal structure and density functional theory (DFT) calculations.
RESUMEN
BACKGROUND: To determine relative fixation strengths of a single lateral locking plate, a double construct of a locking plate, and a tibial nail used in treatment of proximal tibial extra-articular fractures. METHODS: Three groups of composite tibial synthetic bones consisting of 5 specimens per group were included: lateral plating (LP) using a locking compression plate-proximal lateral tibia (LCP-PLT), double plating (DP) using a LCP-PLT and a locking compression plate-medial proximal tibia, and intramedullary nailing (IN) using an expert tibial nail. To simulate a comminuted fracture model, a gap osteotomy measuring 1 cm was created 8 cm below the knee joint. For each tibia, a minimal preload of 100 N was applied before loading to failure. A vertical load was applied at 25 mm/min until tibial failure. RESULTS: Under axial loading, fixation strength of DP (14,387.3 N; standard deviation [SD], 1,852.1) was 17.5% greater than that of LP (12,249.3 N; SD, 1,371.6), and 60% less than that of IN (22,879.6 N; SD, 1,578.8; p < 0.001, Kruskal-Wallis test). For ultimate displacement under axial loading, similar results were observed for LP (5.74 mm; SD, 1.01) and DP (4.45 mm; SD, 0.96), with a larger displacement for IN (5.84 mm; SD, 0.99). The median stiffness values were 2,308.7 N/mm (range, 2,147.5 to 2,521.4 N/mm; SD, 165.42) for the LP group, 4,128.2 N/mm (range, 3,028.1 to 4,831.0 N/mm; SD, 832.88) for the DP group, and 5,517.5 N/mm (range, 3,933.1 to 7,078.2 N/mm; SD, 1,296.19) for the IN group. CONCLUSIONS: During biomechanical testing of a simulated comminuted proximal tibial fracture model, the DP proved to be stronger than the LP in terms of ultimate strength. IN proved to be the strongest; however, for minimally invasive osteosynthesis, which may be technically difficult to perform using a nail, the performance of the DP construct may lend credence to the additional use of a medial locking plate.
Asunto(s)
Fijación Interna de Fracturas/instrumentación , Fracturas Conminutas/cirugía , Fracturas de la Tibia/cirugía , Fenómenos Biomecánicos , Clavos Ortopédicos , Placas Óseas , Fracturas Conminutas/fisiopatología , Humanos , Modelos Anatómicos , Fracturas de la Tibia/fisiopatologíaRESUMEN
Cubital tunnel syndrome is one of the most frequently occurring compression neuropathy in the upper limb next to carpal tunnel syndrome. Recent minimal invasive technique has prompted us to gain clinical experience with simple in situ decompression with minimal skin incision for idiopathic cubital tunnel syndrome. Sixty six consecutive patients with cubital tunnel syndrome were treated using minimal skin incision technique. The mean age of the patients was 49.7 (range: 15-77) years and average follow up period was 23.9 months (range: 12-60 months). The severity of ulnar neuropathy was classified according to the McGowan classification: there were 17 in grade I, 47 in grade II and 2 in grade III. A preoperative nerve conduction study was done by inching method, which revealed motor conduction delay around the medial epicondyle. All operations were carried out in a day surgery unit under local anesthetics. The postoperative outcome was evaluated by Messina classification. The mean duration of the operation was 12 minutes. The technique was highly satisfactorily esthetic for all. Over 80% of the patients were completely satisfied with the procedure taking into consideration their symptoms. Postoperative outcome measures and patient satisfactions (pain, return to normal activities and work, scar and pillar tenderness) were comparable with published series of anterior transposition. The overall satisfactory results were recorded 81% in the patients of McGowan stage I and II. There were 2 cases of hematoma as a postoperative complication. This procedure is comparably effective alternative which involves less surgical trauma, morbidity and rehabilitation time with good surgical outcomes especially in mild and moderate degrees. Minimal skin incision is a simple, safe and effective method to treat patients with idiopathic cubital tunnel syndrome.
Asunto(s)
Síndrome del Túnel Cubital/cirugía , Descompresión Quirúrgica/métodos , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Procedimientos Quirúrgicos Mínimamente Invasivos , Complicaciones Posoperatorias , Adulto JovenAsunto(s)
Artroplastia , Síndrome del Túnel Cubital/etiología , Articulación del Codo/cirugía , Osteoartritis/cirugía , Complicaciones Posoperatorias/etiología , Neuropatías Cubitales/etiología , Síndrome del Túnel Cubital/diagnóstico por imagen , Síndrome del Túnel Cubital/cirugía , Descompresión Quirúrgica , Humanos , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/diagnóstico por imagen , Complicaciones Posoperatorias/cirugía , Rango del Movimiento Articular/fisiología , Reoperación , Neuropatías Cubitales/diagnóstico por imagen , Neuropatías Cubitales/cirugía , UltrasonografíaRESUMEN
Twenty-four unstable tibial fractures were stabilized with a narrow limited contact-dynamic compression plate inserted using a percutaneous plating technique under fluoroscopic guidance. The major indication for this technique was a tibial fracture for which intramedullary nailing would be difficult. There were 16 proximal or distal metaphyseal fractures and 5 segmental fractures in adults and 3 mid-shaft fractures in adolescents who still had an open physis. Of the 24 fractures, 22 healed without a second procedure; the two failures included one that required an early bone graft for severe comminution and another with a superficial infection that healed after early removal of the plate. There were no other infections. There were three cases of screw breakage, but they did not require a further procedure. At the final follow-up, one patient had healed with 5 degrees varus alignment and another with 10 degrees external rotation. All the patients had good knee or ankle function. We are confident that the percutaneous plating technique to treat unstable tibial fractures for which intramedullary nailing would be difficult will prove to be an alternative stabilization method, as it avoids the risk of infection or soft tissue compromise.