RESUMEN
Prenatal exposure to total mercury (T-Hg) comes from both natural and anthropogenic sources. T-Hg can cross the blood-brain and placental barriers, and may be associated with future neurological and physiological dysfunctions. Scalp hair is an optimal and non-invasive indicator of chronic T-Hg exposure. As part of the Maternal-Infant Research on Environmental Chemicals (MIREC) Study, hair samples from 350 women were collected within weeks after giving birth, to determine temporal variations in T-Hg levels from preconception to delivery, and to compare these levels to corresponding levels measured in other matrices (maternal and umbilical cord blood, and infant's meconium). A maximum of 12 one-cm hair segments were cut starting at the scalp; segments closer to the scalp reflected recent exposure (within the last month). For proper comparison, the hair segments were matched with the collection dates for other matrices. GM hair T-Hg levels greatly decreased during pregnancy, from 0.26 µg g-1 (preconception or full-length hair) to 0.18 µg g-1 (at delivery or segments closer to the scalp). A similar decreasing trend was found for T-Hg in maternal blood: 1st trimester (0.60 µg L-1) to 3rd trimester (0.47 µg L-1). The median hair-to-blood ratios of T-Hg levels varied from 364 (1st trimester), to 408 (3rd trimester), to 229 (cord blood). Very low T-Hg levels were detected in meconium. Mercury levels in blood and hair correlated with consumption of large predatory fish.
Asunto(s)
Mercurio , Mujeres Embarazadas , Animales , Exposición a Riesgos Ambientales , Femenino , Sangre Fetal/química , Cabello/química , Humanos , Lactante , Recién Nacido , Exposición Materna , Meconio/química , Mercurio/análisis , EmbarazoRESUMEN
Given the susceptibility of the fetus to toxicants, it is important to estimate their exposure. Approximately 2000 pregnant women were recruited in 2008-2011 from 10 cities across Canada. Cd, Pb, Mn and total Hg were measured in maternal blood from the 1st and 3rd trimesters, umbilical cord blood, and infant meconium. Nutrient intakes of vitamin D, iron, and calcium (Ca) were assessed using a food frequency questionnaire and a dietary supplement questionnaire. Median concentrations in 1st trimester maternal blood (n = 1938) were 0.20, 8.79 and 0.70 µg/L for Cd, Mn and Hg, respectively, and 0.60 µg/dL for Pb. While the median difference between the paired 1st and 3rd trimester concentrations of Cd was 0, there was a significant decrease in Pb (0.04 µg/dL) and Hg (0.12 µg/L) and an increase in Mn (3.30 µg/L) concentrations over the course of the pregnancy. While Cd was rarely detected in cord blood (19%) or meconium (3%), median Pb (0.77 µg/dL), Mn (31.87 µg/L) and Hg (0.80 µg/L) concentrations in cord blood were significantly higher than in maternal blood. Significant negative associations were observed between estimated Ca intake and maternal Cd, Pb, Mn and Hg, as well as cord blood Pb. Vitamin D intake was associated with lower maternal Cd, Pb, and Mn as well as Pb in cord blood. Even at current metal exposure levels, increasing dietary Ca and vitamin D intake during pregnancy may be associated with lower maternal blood Pb and Cd concentrations and lower Pb in cord blood.
Asunto(s)
Cadmio/sangre , Sangre Fetal/efectos de los fármacos , Plomo/sangre , Manganeso/sangre , Exposición Materna , Mercurio/sangre , Adulto , Calcio/química , Canadá , Estudios de Cohortes , Femenino , Humanos , Lactante , Iones , Límite de Detección , Embarazo , Encuestas y Cuestionarios , Vitamina D/químicaRESUMEN
BACKGROUND: Manganese neurotoxicity is well documented in individuals occupationally exposed to airborne particulates, but few data are available on risks from drinking-water exposure. OBJECTIVE: We examined associations of exposure from concentrations of manganese in water and hair with memory, attention, motor function, and parent- and teacher-reported hyperactive behaviors. METHODS: We recruited 375 children and measured manganese in home tap water (MnW) and hair (MnH). We estimated manganese intake from water ingestion. Using structural equation modeling, we estimated associations between neurobehavioral functions and MnH, MnW, and manganese intake from water. We evaluated exposure-response relationships using generalized additive models. RESULTS: After adjusting for potential confounders, a 1-SD increase in log10 MnH was associated with a significant difference of -24% (95% CI: -36, -12%) SD in memory and -25% (95% CI: -41, -9%) SD in attention. The relations between log10 MnH and poorer memory and attention were linear. A 1-SD increase in log10 MnW was associated with a significant difference of -14% (95% CI: -24, -4%) SD in memory, and this relation was nonlinear, with a steeper decline in performance at MnW > 100 µg/L. A 1-SD increase in log10 manganese intake from water was associated with a significant difference of -11% (95% CI: -21, -0.4%) SD in motor function. The relation between log10 manganese intake and poorer motor function was linear. There was no significant association between manganese exposure and hyperactivity. CONCLUSION: Exposure to manganese in water was associated with poorer neurobehavioral performances in children, even at low levels commonly encountered in North America.
Asunto(s)
Déficit de la Atención y Trastornos de Conducta Disruptiva/epidemiología , Manganeso/efectos adversos , Contaminantes Químicos del Agua/efectos adversos , Abastecimiento de Agua , Déficit de la Atención y Trastornos de Conducta Disruptiva/inducido químicamente , Niño , Exposición a Riesgos Ambientales/estadística & datos numéricos , Femenino , Cabello/química , Humanos , Masculino , Manganeso/análisis , Quebec/epidemiología , Contaminantes Químicos del Agua/análisisRESUMEN
Bisphenol A (BPA) and phthalates are endocrine disruptors possibly linked to adverse reproductive and neurodevelopmental outcomes. These chemicals have commonly been measured in urine in population surveys; however, such data are limited for large populations of pregnant women, especially for the critical first trimester of pregnancy. The aim of the study was to measure BPA and phthalate metabolites in first trimester urine samples collected in a large national-scale pregnancy cohort study and to identify major predictors of exposure. Approximately 2000 women were recruited in the first trimester of pregnancy from ten sites across Canada. A questionnaire was administered to obtain demographic and socio-economic data on participants and a spot urine sample was collected and analyzed for total BPA (GC-MS/MS) and 11 phthalate metabolites (LC-MS/MS). The geometric mean (GM) maternal urinary concentration of total BPA, uncorrected for specific gravity, was 0.80 (95% CI 0.76-0.85) µg/L. Almost 88% of the women had detectable urinary concentrations of BPA. An analysis of urinary concentrations of BPA by maternal characteristics with specific gravity as a covariate in the linear model showed that the geometric mean concentrations: (1) decreased with increasing maternal age, (2) were higher in current smokers or women who quit during pregnancy compared to never smokers, and (3) tended to be higher in women who provided a fasting urine sample and who were born in Canada, and had lower incomes and education. Several of the phthalate metabolites analyzed were not prevalent in this population (MCHP, MMP, MiNP, MOP), with percentages detectable at less than 15%. The phthalate metabolites with the highest measured concentrations were MEP (GM: 32.02 µg/L) and MnBP (GM: 11.59 µg/L). MBzP urinary concentrations decreased with maternal age but did not differ by time of urine collection; whereas the DEHP metabolites tended to be higher in older women and when the urine was collected later in the day. This study provides the first biomonitoring results for the largest population of pregnant women sampled in the first trimester of pregnancy. The results indicate that exposure among this population of pregnant women to these chemicals is comparable to or even lower than that observed in a Canadian national population-based survey.
Asunto(s)
Compuestos de Bencidrilo/orina , Contaminantes Ambientales/orina , Exposición Materna , Fenoles/orina , Ácidos Ftálicos/orina , Adolescente , Adulto , Compuestos de Bencidrilo/metabolismo , Canadá , Cromatografía de Gases , Cromatografía Líquida de Alta Presión , Estudios de Cohortes , Demografía , Contaminantes Ambientales/química , Contaminantes Ambientales/metabolismo , Femenino , Humanos , Fenoles/metabolismo , Ácidos Ftálicos/metabolismo , Embarazo , Primer Trimestre del Embarazo , Fumar , Encuestas y Cuestionarios , Espectrometría de Masas en Tándem , Adulto JovenRESUMEN
OBJECTIVES: We present total mercury (THg) in blood of Canadians 6-79 years of age from the first to-date nationally-representative survey, the Canadian Health Measures Survey (CHMS). This analysis is particularly relevant in the context of recent changes to Health Canada's blood Hg guidance values. METHODS: We used data from cycle 1 (2007-2009) of the CHMS, in particular focussing on 5,319 respondents aged 6-79 years for whom blood THg data were available. We calculated descriptive statistics of blood Hg levels and analyzed the associations between blood THg levels and relevant variables. We also compared blood THg levels to existing Health Canada blood Hg guidance values. RESULTS: The geometric mean blood THg level of the Canadian population was 0.69 µg/L (95% CI 0.56-0.86 µg/L). The majority (97.8%) of Canadian women aged 16-49 years, including pregnant women, had blood Hg values below the provisional HC blood guidance value of 8 µg/L. Mean blood THg levels were significantly higher in the "other or mixed" (1.14 µg/L, p=0.0003) and Asian (1.41 µg/L, p=0.0476) groups compared to those who self-identified as solely Caucasian (0.62 µg/L). There was however no significant difference between participants self-identified as Aboriginal Canadians versus Caucasians (0.56 µg/L vs 0.62 µg/L, p=0.2902).Fish and shellfish consumption significantly influenced blood Hg levels, as did alcohol consumption and the presence of dental amalgams. CONCLUSION: This paper presents the first description of blood THg levels in the general Canadian population. These findings will serve as a national baseline for monitoring future changes should they arise.
Asunto(s)
Mercurio/sangre , Adolescente , Adulto , Anciano , Canadá , Niño , Femenino , Guías como Asunto , Encuestas Epidemiológicas , Humanos , Masculino , Persona de Mediana Edad , Embarazo , Adulto JovenRESUMEN
BACKGROUND: The Maternal-Infant Research on Environmental Chemicals (MIREC) Study was established to obtain Canadian biomonitoring data for pregnant women and their infants, and to examine potential adverse health effects of prenatal exposure to priority environmental chemicals on pregnancy and infant health. METHODS: Women were recruited during the first trimester from 10 sites across Canada and were followed through delivery. Questionnaires were administered during pregnancy and post-delivery to collect information on demographics, occupation, life style, medical history, environmental exposures and diet. Information on the pregnancy and the infant was abstracted from medical charts. Maternal blood, urine, hair and breast milk, as well as cord blood and infant meconium, were collected and analysed for an extensive list of environmental biomarkers and nutrients. Additional biospecimens were stored in the study's Biobank. The MIREC Research Platform encompasses the main cohort study, the Biobank and follow-up studies. RESULTS: Of the 8716 women approached at early prenatal clinics, 5108 were eligible and 2001 agreed to participate (39%). MIREC participants tended to smoke less (5.9% vs. 10.5%), be older (mean 32.2 vs. 29.4 years) and have a higher education (62.3% vs. 35.1% with a university degree) than women giving birth in Canada. CONCLUSIONS: The MIREC Study, while smaller in number of participants than several of the international cohort studies, has one of the most comprehensive datasets on prenatal exposure to multiple environmental chemicals. The biomonitoring data and biological specimen bank will make this research platform a significant resource for examining potential adverse health effects of prenatal exposure to environmental chemicals.
Asunto(s)
Contaminantes Ambientales/efectos adversos , Bienestar del Lactante , Exposición Materna/efectos adversos , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Adolescente , Adulto , Biomarcadores , Canadá , Estudios de Cohortes , Exposición a Riesgos Ambientales/efectos adversos , Monitoreo del Ambiente/métodos , Femenino , Humanos , Lactante , Masculino , Embarazo , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Biomonitoring is used increasingly as an indicator and quantitative measure of exposure; however, there is a large gap in interpreting and communicating biomonitoring results to study participants. Two separate, national biomonitoring initiatives are under way in Canada; the household recruitment-based Canadian Health Measures Survey (CHMS) and the clinic recruitment-based Maternal-Infant Research on Environmental Chemicals (MIREC) Study. The CHMS provides participants with the option to receive all their results, but this option is not provided to MIREC participants. The approach to reporting results to participants depends on the availability of reference ranges and guidelines for which tissue concentrations may be interpreted as being elevated or associated with increased health risks, how participants are recruited, unique vulnerabilities of the population, legislation governing access to personal information, and decisions of research ethics committees. It is the researchers' responsibility to present the best case for their approach and, once the decision has been made, to inform participants about access to their results through the consent process.
Asunto(s)
Exposición a Riesgos Ambientales/análisis , Monitoreo del Ambiente/ética , Experimentación Humana/ética , Sujetos de Investigación , Adolescente , Adulto , Beneficencia , Biomarcadores/sangre , Biomarcadores/orina , Canadá , Niño , Recolección de Datos/métodos , Revelación , Monitoreo del Ambiente/métodos , Contaminantes Ambientales/análisis , Femenino , Programas de Gobierno , Encuestas Epidemiológicas , Humanos , Entrevistas como Asunto , Estilo de Vida , Masculino , Persona de Mediana Edad , Autonomía Personal , Vigilancia de la Población/métodos , Relaciones Profesional-Paciente/ética , Valores de Referencia , Sujetos de Investigación/psicología , Medición de Riesgo/ética , Medición de Riesgo/métodos , Factores Socioeconómicos , Adulto JovenRESUMEN
BACKGROUND: Manganese is an essential nutrient, but in excess it can be a potent neurotoxicant. Despite the common occurrence of manganese in groundwater, the risks associated with this source of exposure are largely unknown. OBJECTIVES: Our first aim was to assess the relations between exposure to manganese from drinking water and children's intelligence quotient (IQ). Second, we examined the relations between manganese exposures from water consumption and from the diet with children's hair manganese concentration. METHODS: This cross-sectional study included 362 children 6-13 years of age living in communities supplied by groundwater. Manganese concentration was measured in home tap water (MnW) and children's hair (MnH). We estimated manganese intake from water ingestion and the diet using a food frequency questionnaire and assessed IQ with the Wechsler Abbreviated Scale of Intelligence. RESULTS: The median MnW in children's home tap water was 34 µg/L (range, 1-2,700 µg/L). MnH increased with manganese intake from water consumption, but not with dietary manganese intake. Higher MnW and MnH were significantly associated with lower IQ scores. A 10-fold increase in MnW was associated with a decrease of 2.4 IQ points (95% confidence interval: -3.9 to -0.9; p < 0.01), adjusting for maternal intelligence, family income, and other potential confounders. There was a 6.2-point difference in IQ between children in the lowest and highest MnW quintiles. MnW was more strongly associated with Performance IQ than Verbal IQ. CONCLUSIONS: The findings of this cross-sectional study suggest that exposure to manganese at levels common in groundwater is associated with intellectual impairment in children.
Asunto(s)
Exposición a Riesgos Ambientales/estadística & datos numéricos , Inteligencia/efectos de los fármacos , Manganeso/toxicidad , Estudiantes/estadística & datos numéricos , Contaminantes Químicos del Agua/toxicidad , Adolescente , Niño , Estudios Transversales , Dieta/estadística & datos numéricos , Exposición a Riesgos Ambientales/análisis , Femenino , Cabello/metabolismo , Humanos , Masculino , Manganeso/análisis , Manganeso/metabolismo , Encuestas y Cuestionarios , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/metabolismo , Abastecimiento de Agua/análisisRESUMEN
Exposure to methylmercury (MeHg) from fish and marine mammal consumption continues to present a public health concern. To date, developmental neurotoxicity is the most sensitive health outcome, forming the basis for health-risk assessments and the derivation of biomonitoring guidance values. This article summarizes existing Health Canada MeHg blood guidance values for general population and expands them to include a harmonized provisional interim blood guidance value of 8 microg/L based on the existing provisional Tolerable Daily Intake for children, pregnant women and women of childbearing age. Associated public health actions, according to age, sex, and level of exposure are recommended.
Asunto(s)
Exposición a Riesgos Ambientales/efectos adversos , Conducta Alimentaria , Mamíferos , Compuestos de Metilmercurio/sangre , Salud Pública , Alimentos Marinos/efectos adversos , Adolescente , Adulto , Anciano , Animales , Canadá , Niño , Femenino , Humanos , Masculino , Compuestos de Metilmercurio/toxicidad , Persona de Mediana Edad , Guías de Práctica Clínica como Asunto , Valores de Referencia , Medición de Riesgo , Adulto JovenRESUMEN
An international workshop titled "Assessing Endocrine-Related Endpoints within the First Years of Life" was held 30 April-1 May 2007, in Ottawa, Ontario, Canada. Representatives from a number of pregnancy cohort studies in North America and Europe presented options for measuring various endocrine-sensitive endpoints in early life and discussed issues related to performing and using those measures. The workshop focused on measuring reproductive tract developmental endpoints [e.g., anogenital distance (AGD)], endocrine status, and infant anthropometry. To the extent possible, workshop participants strove to develop or recommend standardized measurements that would allow comparisons and pooling of data across studies. The recommended outcomes include thigh fat fold, breast size, vaginal cytology, AGD, location of the testis, testicular size, and growth of the penis, with most of the discussion focusing on the genital exam. Although a number of outcome measures recommended during the genital exam have been associated with exposure to endocrine-disrupting chemicals, little is known about how predictive these effects are of later reproductive health or other chronic health conditions.
Asunto(s)
Desarrollo Infantil/fisiología , Sistema Endocrino/efectos de los fármacos , Exposición a Riesgos Ambientales/efectos adversos , Antropometría , Investigación Biomédica/tendencias , Pesos y Medidas Corporales , Mama/anomalías , Mama/crecimiento & desarrollo , Sistema Endocrino/crecimiento & desarrollo , Determinación de Punto Final , Femenino , Genitales Femeninos/anomalías , Genitales Femeninos/crecimiento & desarrollo , Genitales Masculinos/anomalías , Genitales Masculinos/crecimiento & desarrollo , Hormonas Esteroides Gonadales/sangre , Humanos , Lactante , Recién Nacido , Masculino , Caracteres Sexuales , Hormonas Tiroideas/sangreRESUMEN
Although it has been established that mercury (Hg) can be detected in single hair strands using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), calibration remains a challenge due to the lack of well-characterized matrix-matched standards. We concurrently evaluated two strategies for quantifying Hg signals in single hair strands using LA-ICP-MS. The main objective was to obtain time-resolved Hg concentrations in single hair strands of fish-eaters that would correspond to the changes of their body burden over time. Experiments were conducted using hair samples collected from 10 individuals. The first experiment involved the construction of a calibration curve with four powdered hair standard reference materials (SRMs) with a range of Hg concentrations (0.573-23.2 mg/kg). An internal standard, sulfur, as 34S, was applied to correct for ablation efficiency for both the hair strands and the SRMs. Results showed a linear relationship (R2 = 0.899) between the ratio of 202Hg to 34S obtained by LA-ICP-MS and the certified total Hg concentration in the SRMs. Using this calibration curve, average Hg concentrations of 10 shots within a 1-cm segment of a hair strand were calculated and then compared to the total Hg concentrations in the matched 1-cm segment as measured by cold vapor atomic absorption spectrometry (CV-AAS). A significant difference (p < 0.05) was observed. The difference could be attributed to the highly variable ablation/sampling process caused by the use of the laser on the hair powder SRM pellets and the difference in the physical properties of the SRMs. An alternative approach was adopted to quantify consecutive 202Hg to 34S ratios by calibrating the signals against the average Hg concentration of the matched hair segment as measured by CV-AAS. Consecutive daily Hg deposition in single hairs of fish eaters was determined. Results showed that apparent daily changes in Hg concentrations within a hair segment that corresponds to 1 month of hair growth. In addition, a significant decreasing or increasing time-trend was observed. The difference between the minimum and maximum Hg concentration within each individual corresponded to a change of 26-40%. Our results showed that LA-ICP-MS can be used to reconstruct time-resolved Hg exposure in micrometer segments of a single hair strand.
Asunto(s)
Dieta , Monitoreo del Ambiente/métodos , Contaminantes Ambientales/análisis , Cabello/química , Mercurio/análisis , Animales , Carga Corporal (Radioterapia) , Calibración , Peces , Humanos , Rayos Láser , Espectrometría de Masas/métodos , Espectrofotometría Atómica , Factores de TiempoRESUMEN
Hair samples continue to be used extensively for biomonitoring of mercury (Hg) exposure. Routine methods require a bundle of 100-150 hair strands and involve chemical digestion. Recently, Hg analyzers that combine combustion, gold amalgamation, and atomic absorption spectrometry (C-GA-AAS) became commercially available. This method was shown to provide quick and sensitive measurements in solid samples such as hair. The objective of this study was to validate C-GA-AAS for measuring total Hg in single hair strands as an alternative method for Hg biomonitoring. Hair samples from 12 women with a wide range of Hg exposure were obtained from two projects conducted in Brazil and Canada. A 1:1 relationship was observed between C-GA-AAS and the established cold vapor atomic absorption spectrometry (CV-AAS) for analysis of 1-cm segments from a bundle of hair. For individual hair variability, the average relative standard deviation (RSD) of Hg between hair strands was 6.5 +/- 2.8%, thus justifying the use of single hair strand for biomonitoring. With a limit of quantification of 0.10 ng of total Hg, a single hair strand can be used to assess monthly exposure. This technique will facilitate routine biomonitoring and thus help prevent Hg poisoning among the public.
Asunto(s)
Monitoreo del Ambiente/métodos , Cabello/química , Mercurio/análisis , Brasil , Canadá , Femenino , Oro , Humanos , Reproducibilidad de los Resultados , Espectrofotometría Atómica/métodosRESUMEN
There is a rising global concern with regard to mercury (Hg) exposure among coastal populations. Two communities on the Bay of Fundy (New Brunswick, Canada) were assessed by hair monitoring and dietary methods. Average concentration of total Hg in hair was 0.70+/-0.55 mg/kg (N=91) at Grand Manan and 0.42+/-0.15 mg/kg (N=52) at St. Andrews/St. Stephen. Average daily consumption of fresh fish and shellfish was 50+/-40 g/day for Grand Manan and 19+/-19 g/day for St. Andrews/St. Stephen. Average daily total Hg intake estimated from the food frequency and 24-h recall questionnaires was 0.05+/-0.04 microg Hg/kg bw/day at Grand Manan and 0.03+/-0.04 microg Hg/kg bw/day at St. Andrews/St. Stephen. A significant correlation (r=0.47, P=0.002) between Hg intake and hair was observed for Grand Manan. Low Hg intakes and body burden can be attributed to the low Hg levels found in the species commonly consumed: haddock, canned tuna, lobster, and pollock (all below 0.2 microg/g wet weight). The results showed that Hg exposure in these Canadian coastal communities is low; fish with higher levels of Hg (shark, tuna, swordfish, pickerel, and bass) are not consumed locally.
Asunto(s)
Exposición a Riesgos Ambientales , Cabello/metabolismo , Mercurio/farmacocinética , Contaminantes Químicos del Agua/farmacocinética , Adolescente , Adulto , Factores de Edad , Anciano , Animales , Encuestas sobre Dietas , Femenino , Productos Pesqueros/efectos adversos , Contaminación de Alimentos , Cabello/química , Humanos , Inuk , Masculino , Mercurio/análisis , Persona de Mediana Edad , Nuevo Brunswick , Alimentos Marinos/efectos adversos , Estaciones del Año , Factores Sexuales , Espectrofotometría Atómica , Encuestas y Cuestionarios , Contaminantes Químicos del Agua/análisisRESUMEN
Concentrations of DDT and its metabolites were measured in water, plants, invertebrates, and fish from lagoons in the Okavango Delta, Botswana (Africa), where DDT has been used for approximately 50 years. The sampling area was sectioned to distinguish spraying for malaria and for African sleeping sickness. Average concentrations of total DDT (sum of DDT and its metabolites) in the Okavango ranged from 0.009 ng/L in water to 18.76 ng/g wet weight in fish. These levels are approximately 1% of those found in piscivorous fish from temperate North America. The dichlorodiphenyl ethylene (DDE) metabolite was the most abundant fraction of total DDT. Although total DDT concentrations were higher in areas treated for malaria than areas treated for sleeping sickness, these concentrations were likely driven by factors other than the historic application of the pesticide. Equilibration with air concentrations is the most likely explanation for these levels. Since the mean annual temperature exceeds the temperature of vaporization of DDT, this research points to the need for reliable transport models. Our results showed that total DDT concentration in fish was best explained by lipid content of the fish and trophic position inferred by delta15N, regardless of DDT application history in those areas. The reservoir above Gaborone Dam, an area downstream of the Okavango but where DDT had not been used, was sampled to compare total DDT levels to the treated areas. The two species (a herbivorous threespot talapia and the omnivorous sharptooth catfish) from Gaborone had levels higher than those found in the Okavango Delta, but these differences can again be explained using trophic position inferred by delta15N rather than by fish size or location.