Ageing Safely in the Digital Era: A New Unobtrusive Activity Monitoring Framework Leveraging on Daily Interactions with Hand-Operated Appliances.

Supporting the elderly to maintain their independence, safety, and well-being through Active Assisted Living (AAL) technologies, is gaining increasing momentum. Recently, Non-intrusive Load Monitoring (NILM) approaches have become the focus of these technologies due to their non-intrusiveness and reduced price. Whilst some research has been carried out in this respect; it still is challenging to design systems considering the heterogeneity and complexity of daily routines. Furthermore, scholars gave little attention to evaluating recent deep NILM models in AAL applications. We suggest a new interactive framework for activity monitoring based on custom user-profiles and deep NILM models to address these gaps. During evaluation, we consider four different deep NILM models. The proposed contribution is further assessed on two households from the REFIT dataset for a period of one year, including the influence of NILM on activity monitoring. To the best of our knowledge, the current study is the first to quantify the error propagated by a NILM model on the performance of an AAL solution. The results achieved are promising, particularly when considering the UNET-NILM model, a multi-task convolutional neural network for load disaggregation, that revealed a deterioration of only 10% in the f1-measure of the framework's overall performance.

Disseminating ambient assisted living in rural areas.

The smart home, ambient intelligence and ambient assisted living have been intensively researched for decades. Although rural areas are an important potential market, because they represent about 80% of the territory of the EU countries and around 125 million inhabitants, there is currently a lack of applicable AAL solutions. This paper discusses the theoretical foundations of AAL in rural areas. This discussion is underlined by the achievements of the empirical field study, Casa Vecchia, which has been carried out over a four-year period in a rural area in Austria. The major goal of Casa Vecchia was to evaluate the feasibility of a specific form of AAL for rural areas: bringing AAL technology to the homes of the elderly, rather than moving seniors to special-equipped care facilities. The Casa Vecchia project thoroughly investigated the possibilities, challenges and drawbacks of AAL related to this specific approach. The findings are promising and somewhat surprising and indicate that further technical, interactional and socio-psychological research is required to make AAL in rural areas reasonable in the future.

A functional germline variant in GLI1 implicates hedgehog signaling in clinical outcome of stage II and III colon carcinoma patients.

PURPOSE: Cumulating evidence indicates that germline variants in the Wnt, Notch, and Hedgehog pathways are involved in colon carcinoma progression and metastasis. We investigated germline polymorphisms in a comprehensive panel of Wnt, Notch, and Hedgehog pathway genes to predict time to recurrence (TTR) and overall survival in patients with stage II and III colon carcinoma. EXPERIMENTAL DESIGN: A total of 742 consecutively collected patients with stage II and III colon carcinoma were included in this retrospective study. Genomic DNA was analyzed for 18 germline polymorphisms in Wnt, Notch, and Hedgehog pathway genes (SFRP, DKK 2 and 3, AXIN2, APC, MYC, TCF7L2, NOTCH2, and GLI1) by TaqMan 5'-exonuclease assays. RESULTS: In univariate analysis, the homozygous mutant variant of GLI1 rs2228226 G>C was significantly associated with decreased TTR in a recessive genetic model after adjustment for multiple testing [HR = 2.35; confidence interval (95% CI), 1.48-3.74; P < 0.001] and remained significant in multivariate analysis including clinical stage, lymphovascular-, vascular-, and perineural-invasion (HR = 2.43; CI 95%, 1.52-3.87; P < 0.001). In subanalyses, the association was limited to patients with surgery alone (HR = 3.21; CI 95%, 1.59-6.49; P = 0.001), in contrast with patients with adjuvant chemotherapy (HR = 0.82; CI 95%, 0.35-1.95; P = 0.657). When the subgroup of patients with "high-risk" GLI1 rs2228226 C/C genotype was analyzed, no benefit of adjuvant 5-fluorouracil-based chemotherapy could be found. CONCLUSION: This is the first study identifying GLI1 rs2228226 G>C as an independent prognostic marker in patients with stage II and III colon carcinoma. Prospective studies are warranted to validate our findings.

Molecular profiling of yeasts isolated during spontaneous fermentations of Austrian wines.

The aim of the present study was to evaluate the autochthonous yeast population during spontaneous fermentations of grape musts in Austrian wine-producing areas. Investigation of genomic and genetic variations among wine yeasts was a first step towards a long-term goal of selecting strains with valuable enological properties typical for this geographical region. An approach, combining sequences of the D1/D2 domain of the 26S rRNA gene and random amplified polymorphic DNA fingerprinting, was used to characterize yeasts at the species level, whereas the differentiation of Saccharomyces strains was accomplished by amplified fragment length polymorphism fingerprinting. At the beginning of fermentation, representatives of nine genera were identified, with Hanseniaspora and Metschnikowia species characterized most frequently. Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum strains, which were identified throughout the entire fermentation process, showed a high level of genetic diversity. A number of S. cerevisiae strains were common at multiple wineries, but a wide range of strains with characteristic profiles were characterized at individual locations. This biodiversity survey represents a contribution to the investigation and preservation of genetic diversity of biotechnologically relevant yeasts in Austrian wine-making areas.

[Epidemiology and nosology of anisakiosis, a rather rare helminthozoonosis in Central Europe--two case reports]. / Epidemiologie und Nosologie der Anisakiose, einer in Mitteleuropa selten diagnostizierten Helminthozoonose--zwei Fallberichte.

Two cases of anisakiosis are reported. Two male patients (54 and 58 years old) had spent their holidays in Alaska for salmon fishing at the end of July 2006 and consumed the self caught cold smoked salmon. Back in Austria both patients suffered from abdominal pain within 6 and 15 days respectively, after consumption and received in-patient treatment (patient 1: subileus; patient 2: ileus). Patient 1 received aprednisolon alone and recovered within 3 days, patient 2, however, was treated surgically (ileus) and suffered from an ARDS and an insufficiency of anastomosis during postoperative intensive therapy, additionally he received hydrocortisone. Both patients recovered completely. The diagnosis of anisakiosis was primarily based on the common anamnesis (consumption of cold smoked salmon) and the detection of eosinophilia in the differential blood picture. The diagnosis was confirmed by the detection of specific antibodies against Anisakis antigen in the serum of patient 1, the morphological determination and molecularbiological characterization (PCR, sequence analysis) of Anisakis simplex s. str. larvae found in parts of the consumed salmon as well as by the detection of Anisakis DNA in the resected ileum by a nested PCR. These two cases of anisakiosis are the first documented cases in Austria.

Genetically different wine yeasts isolated from Austrian vine-growing regions influence wine aroma differently and contain putative hybrids between Saccharomyces cerevisiae and Saccharomyces kudriavzevii.

To evaluate the influence of the genomic properties of yeasts on the formation of wine flavour, genotypic diversity among natural Saccharomyces cerevisiae strains originating from grapes collected in four localities of three Austrian vine-growing areas (Thermenregion: locations Perchtoldsdorf and Pfaffstätten, Neusiedlersee-Hügelland: location Eisenstadt, Neusiedlersee: location Halbturn) was investigated and the aroma compounds produced during fermentation of the grape must of 'Grüner Veltliner' were identified. Amplified fragment length polymorphism analysis (AFLP) showed that the yeast strains cluster in four groups corresponding to their geographical origin. The genotypic analysis and sequencing of the D1/D2 domain of 26S rRNA encoding gene and ITS1/ITS2 regions indicated that the Perchtoldsdorf strains were putative interspecies hybrids between S. cerevisiae and Saccharomyces kudriavzevii. Analysis of the aroma compounds by GS/MS indicated a region-specific influence of the yeasts on the chemical composition of the wines. The aroma compound profiles generated by the Perchtoldsdorf strains were more related to those produced by the Pfaffstätten strains than by the Eisenstadt and Halbturn strains. Similar to the Pfaffstätten yeasts, the putative hybrid strains were good ester producers, suggesting that they may influence the wine quality favourably.

Clinical epidemiology of invasive cutaneous malignant melanoma in the Austrian province Styria in the years 2001-2003 and its relationship with local geographical, meteorological and economic data.

BACKGROUND: Melanoma incidence rates vary within Europe. The highest incidences are reported in Scandinavia, the lowest in the southern parts, but incidences themselves also vary within the different countries. OBJECTIVE: We investigated the incidence of invasive cutaneous melanoma in Styria, a province of Austria, in the years 2001-2003. METHODS: Data from 1,082 patients, 511 males and 571 females (mean age 58.2 years) with primary melanoma were collected. For each patient, information regarding residence was available, and therefore the geographic distribution of melanoma on district level was investigated with particular reference to the mean number of sun hours, mean altitude, number of companies with more than 200 employees and median income. RESULTS: The mean annual incidence (age-standardized rate) was 24.5 per 100,000 (95% CI: 22.4-26.6), lifetime risk 1 in 52. Districts with a higher number of sun hours and higher altitude showed lower melanoma incidences. Higher median income was associated with higher melanoma incidence (p<0.001). CONCLUSION: The high incidence of invasive melanoma in Styria is unclear and a causal relationship between higher income and melanoma incidence remains speculative. Further investigations, especially concerning lifestyle and environmental factors, may unravel additional causative factors.