Comparing robotic and open surgical techniques in gallbladder cancer management: a detailed systematic review and meta-analysis.

This meta-analysis aims to evaluate the safety and oncological outcomes of robotic surgery compared to open surgery in treating gallbladder cancer (GBC). In October 2023, we performed a literature search across major global databases such as PubMed, Embase, and the Cochrane Library. We employed a Review Manager for parameter comparisons. This study has been registered with PROSPERO under the identifier CRD42023476686. Our final meta-analysis incorporated 5 cohort studies, encompassing a total of 353 patients. Compared to the Open Group (OG), the Robotic Group (RG) had reduced intraoperative blood loss (WMD - 217.72 ml, 95% CI - 371.08 to - 64.35; p = 0.005), shorter hospital stay (WMD - 1.80 days, 95% CI - 2.66 to - 0.95; p < 0.0001), and fewer overall complications (OR 0.31, 95% CI 0.10-0.97; p = 0.04). However, there was no significant difference between the two groups in terms of operation duration, postoperative inpatient days, readmission rate, major complications, 1-year postoperative survival, 2-year postoperative survival, and mortality rates. In our study, we found that for patients with gallbladder cancer, robotic radical cholecystectomy offers certain potential advantages over open radical cholecystectomy. This suggests that robotic radical cholecystectomy might be the optimal choice for treating gallbladder cancer. However, further validation from high-quality randomized clinical trials is required.

Simultaneous adsorption of Cd and As by a novel coal gasification slag based composite: Characterization and application in soil remediation.

Cadmium (Cd) and arsenic (As) co-contamination has become increasingly serious in China agricultural soil due to rapid industrialization and urbanization. The opposite geochemical behaviors of Cd and As pose huge challenges for developing a material for their simultaneous immobilization in soils. Coal gasification slag (CGS) as a by-product of coal gasification process, is always dumped into a local landfill, which has a negative impact on environment. Few reports have been available on applying CGS as a material to immobilize simultaneously multiple soil heavy metals. A series of iron-modified coal gasification slag (IGS) composites IGS3/5/7/9/11 (with different pH values) were synthesized by alkali fusion and iron impregnation. After modification, carboxyl groups were activated, and Fe was successfully loaded onto the surface of IGS in the form of FeO and Fe2O3. The IGS7 exhibited the best adsorption capacity with the maximum Cd and As adsorption capacity of 42.72 mg/g and 35.29 mg/g, respectively. The Cd was mainly adsorbed through electrostatic attraction and precipitation, while the As through complexation with iron (hydr)oxides. IGS7 significantly reduced the bioavailability of Cd and As in soil with Cd bioavailability reduced from 1.17 mg/kg to 0.69 mg/kg and As bioavailability reduced from 10.59 mg/kg to 6.86 mg/kg at 1 % IGS7 addition. The Cd and As were all transformed to more stable fractions after IGS7 addition. The acid soluble and reducible Cd fractions were transformed into oxidizable and residual Cd fractions, and the non-specifically and specifically adsorbed As fractions were transformed to amorphous iron oxide-bound As fraction. This study provides valuable references for the application of CGS to the remediation of Cd and As co-contaminated soil.

MiR-92a promotes stem cell-like properties by activating Wnt/ß-catenin signaling in colorectal cancer.

We previously reported the oncogenic function of miR-92a in colorectal cancer. This study identified that miR-92a was upregulated in chemoresistant colorectal cancer cells and tissues. Ectopic expression of miR-92a conferred resistance to 5-fluorouracil-induced apoptosis in vitro, while antagomiR-92a significantly enhanced chemosensitivity in vivo. Moreover, Overexpression of miR-92a promoted the tumor sphere formation and the expression of stem cell markers. MiR-92a overexpression also displayed higher tumourigenesis in vivo. Furthermore, we demonstrated that miR-92a upregulates the Wnt/ß-catenin signaling activity via directly targeting KLF4, GSK3ß and DKK3, which are multiple level negative regulators of the Wnt/ß-catenin signaling cascade. In addition, our results indicate IL-6/STAT3 pathway increases miR-92a expression by directly targeting its promoter, resulting in Wnt/ß-catenin signaling activation and consequent promotion of stem-like phenotypes of colorectal cancer cells. Our present results suggest the essential role of IL-6/STAT3/miR-92a/Wnt/ß-catenin pathway in regulating the stem cell-like traits of colorectal cancer cells and provide a potential target for colorectal cancer therapy.

The complete mitochondrial genome sequence of the Dark-spotted frog Pelophylax nigromaculatus (Amphibia, Anura, Ranidae).

The dark-spotted frog (Pelophylax nigromaculatus) belongs to Ranidae. This species is known from the Russian Far East, central, northern and north-eastern China, the Democratic People's Republic of Korea, the Republic of Korea, and Japan. In this study, the complete mitochondrial genome of P. nigromaculatus was sequenced. The mitogenome was 17 567 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a non-coding control region. As in other vertebrates, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall base composition of the P. nigromaculatus is 29.2% A, 27.4% T, 28.4% C, and 15.0% G. Phylogenetic analysis showed P. nigromaculatus was closely related to P. plancyi and P. chosenicus. The complete mitogenome of P. nigromaculatus can provide important data for the studies on phylogenetic relationship and population genetics to further explore the taxonomic status of this species.