[Antioxidant and anti-inflammatory effect of "vitalong" in hyperlipidemia in rats]. / Antioksidantnoe i protivovospalitel'noe deistvie "vitalonga" pri giperlipidemii u krys.

In experiment on model of the lipidemia in rats the efficiency of new antioxidant drug "Vitalong" was studied. The researches have shown that the drug brakes derivation of free radicals, increases activity of a superoxide scavenger, has antiinflammatory and adaptogenic operation. Vitalong prevents activation of serumal elastasa, locking thus destructive link of the atherogenesis. Ukrainian Ministry of Health permits the drug as a preventive means.

[Clinical use of catomas and biotrit in a combined treatment of patients with chronic neurodermatitis]. / Klinicheskoe primenenie Katomasa i Biotrita v kompleksnom lechenii bol'nykh s khronicheskimi formami neirodermita.

In 170 patients with neurodermatitis, therapeutic benefit was studied of two new antioxidant drug preparations catomas (containing beta-carotene and tocopheroli acetas) and biotrit (extractum from the sprouting wheat). Catomas was used in applications to the area of affection with the aid of phonophoresis, biotrit in injections. It has been shown by the studies made that employment of the above drugs combined with sedatives, desensitizing and anti-inflammatory means of remediation enhances efficiency of the treatments administered, which fact was evidenced by the reduction of the mean duration of the treatment undergone by 4.4 days and by 30% increase in the treatment efficiency. Greater benefit from catomas and biotrit will be seen in those patients having been placed on a general treatment.

[Soybean bacteriological culture media and prospects for their use in clinical bacteriology]. / Soevye bakteriologicheskie pitatel'nye sredy i perspektivy ikh ispol'zovaniia v klinicheskoi bakteriologii.

Agar bacteriological nutrient media are suggested, based on soybean extract subjected to enzymatic hydrolysis and lyophilized native. The growth of test strains, number of grown colonies and their size were virtually the same as after inoculation of these strains in control nutrient media. Soybean salt medium with yolk suspension was tried with good results as elective medium for isolation of staphylococci from clinical material. Commercial manufacture of these media mainly from native soybean extract is proposed.

[Trypsin inhibitors of wheat seedlings infected and treated with salicylic acid]. / Ingibitory tripsina iz infitsirovannykh i obrabotannykh salitsilovoi kislotoi prorostkov pshenitsy.

Using the method of affinity chromatography for trypsin-sepharose, followed by PAGE electrophoresis the component composition of trypsin inhibitors was studied. The inhibitors were extracted from the winter wheat seedlings which were infected with Fusarium fungus and also from the seedlings treated with salicylic acid. Under the action of pathogen not only a quantitative increase in the protein components of trypsin inhibitor but also the appearance of a new component with a molecular weight of 29 kDa are observed. The influence of Fusarium graminearum and salicylic acid on the amino acid composition of trypsin inhibitors has been studied. After the inhibitors purification their antienzyme spectrum was studied along with their influence on the pathogen proteases. It was established that the inhibitors influence only the activity of trypsin and chymotrypsin and do not influence the activity of pepsin, elastases, papain, proteinases from the wheat seedlings. The existence of arginine and absence of lysine was demonstrated in the active centre of the inhibitors.

[The kinetics of the inactivation of the soybean trypsin inhibitor by heat processing]. / Kinetika inaktivatsii soevogo ingibitora tripsina pri teplovoi obrabotke.

The investigations of kinetics of the thermal degradation of the antinutritious substances in the soya beans were carried out. The mathematical analysis of the obtained experimental data helped to determine the kinetic constants D and Z necessary to calculate the time of heat treatment to obtain the minimal value of the inhibitor at any temperature level.

[The use of protease inhibitors in the treatment of burn wounds]. / Ispol'zovanie ingibitorov proteaz v lechenii ozhogovykh ran.

The results of experimental and clinical studies of the effect of contrykal and aminocaproic acid on tissue regeneration in deep burns are presented. In the experiment, by means of planimetric, histologic, histochemical and biochemical methods of investigation, the positive effect of inhibitors on healing of the burn wounds is shown. In treatment of 272 patients with deep burns, it was established that use of protease inhibitors at the phase of regeneration contributed to 2-fold acceleration of marginal epithelization, shortening of time of would preparation to autodermoplasty by 3-4 days, increase in effectiveness of survival of the cutaneous flaps. A possibility of wide clinical use of proteolysis inhibitors predominantly in combination with antibiotics is shown.

[Clinico-enzymologic parallels in patients of reproductive age with benign epithelial ovarian tumors]. / Kliniko-énzimologicheskie paralleli u bol'nykh dobro-kachestvennymi épitelial'nymi opukholiami iaichnikov v reproduktivnom vozraste.

Proteolytic enzymes and their inhibitors have been assayed in sera of 120 patients with benign epithelial ovarian tumors during preoperative menstrual cycles and in 3 years following surgery. The patients showed imbalances in protease inhibitors which failed to be improved by the operative treatment in a proportion of patients. Follow-up of this patient population is recommendable. A serum elastase test was proposed to predict benign epithelial ovarian tumors.

[Correction of mineral and protein metabolism by anabolic preparations and vitamin D2 in rats with dental caries]. / Korrektsiia mineral'nogo i belkovogo obmena pri kariece zubov u krys anabolicheskimi preparatiami i vitaminom D2.

The effect of nerabol, namacyte and vitamin D2 introduction (separately or in combination) on the animal mineral and protein metabolism has been studied in the experiment on 210 white Wistar rats with reproduced caries. Biochemical and radioisotopic investigations have permitted determining the quantitative content of phosphorus, calcium, 14C-glycine in the saliva, blood serum, teeth, jaw bones and femur. The data obtained evidence for the pronounced caries-static effect of the tested preparations connected with their active influence on the mineral and protein metabolism of experimental animals as well as for the expediency to use the above drugs for complex prophylaxis and treatment of dental caries normalizing processes.

[Effect of trypsin and kallikrein (andecalin) on the activity of proteolytic enzymes and their inhibitors in acute experimental pancreatitis]. / Vliianie tripsina i kallikreina (andekalina) na aktivnost' proteoliticheskikh fermentov i ikh ingibitorov pri ostrom éksperimental'nom pankreatite.

Intramuscular administration of trypsin and kallikrein (andecaline) into rats contributed to more favourable development of acute traumatic pancreatitis. Trypsin and andecaline prevented the activation of proteinases in blood serum and pancreas and promoted an increase in content of trypsin inhibitor in blood serum. The proteinases efficiency appears to depend on elevation of general resistance of the organism to the trauma.

[Lipid peroxidation and antioxidant systems in cat periodontal tissues]. / Protsessy perekisnogo okisleniia lipidov i antioksidantnye sistemy v tkaniakh parodonta koshek.

Lipid peroxidation and activity of antioxidant systems were studied in parodontium tissues of 64 cats; maximal activation of lipid peroxidation was detected in gingivitis both in gingiva and in alveolar process. Lipid peroxidation tended to normalization in gingiva only under conditions of light form of parodontitis, while in alveolar process--during parodontitis of middle severity. Deterioration of the antioxidant systems in bones during pathological state was more distinct as compared with gingiva; hence, metabolic alterations, occurred in response to activation of lipid peroxidation in gingivitis, were proceeded in bone structures of alveolar process and after the inflammation decrease in gingiva.

[Use of epsilon-aminocaproic acid for treating granulating burn wounds]. / Primenenie épsilon-aminokapronovoi kisloty dlia lecheniia granuliruiushchikh ozhogovykh ran.

Results of the application of synthetic inhibitor of proteolytic enzymes epsilon-aminocaproic acid in the treatment of granulating burn wounds are described. High effectiveness of the aminocaproic acid in healing of granulating wounds after burns is shown as well as in preparing great granulating burn wounds for autodermoplasty and the take of transplanted autodermic flaps.

[Study of the nutritive value of grain proteins using Tribolium confusum larvae]. / K izucheniiu pishchevoi tsennosti zernovykh belkov pri.

A study was made of the nutrition of Tribolium confusum larvae with the purpose of using them as test-organism for determination of the nutritive value of grain proteins. It was demonstrated that there is a good agreement between the content of lysine in the grain diets containing barley and corn and average daily weight gains among the insects: r = +0,85 and r = +0,75, respectively. It was noted that during nutrition with grain diets containing different levels of proteins, the growth of the insects depended on the protein quality. Experiments on the insects fed diets prepared from different anatomical parts of the grain demonstrated a high nutritive value of germ proteins. Comparative experiments performed with the diets from barley demonstrated a good agreement between average daily weight gains of rats and insect larvae (r = +0.9). The data obtained indicate that the test-organism under consideration can be used for evaluating the nutritive value of grain proteins.