RESUMEN
INTRODUCTION: This study examines bone turnover marker (BTM) variations between bone marrow and peripheral blood in osteoporotic and non-osteoporotic patients. BTMs offer insights into bone remodeling, crucial for understanding osteoporosis. METHODS: A total of 133 patients were categorized into osteoporotic and non-osteoporotic cohorts. BTMs-C-telopeptide cross-linked type 1 collagen (ß-CTX), serum osteocalcin (OC), Procollagen type I N-propeptide (P1NP), 25(OH)D-were measured in bone marrow and peripheral blood. Lumbar spine bone mineral density (BMD) was assessed. RESULTS: Osteoporotic patients exhibited elevated ß-CTX and OC levels in peripheral blood, indicating heightened bone resorption and turnover. ß-CTX levels in osteoporotic bone marrow were significantly higher. Negative correlations were found between peripheral blood ß-CTX and OC levels and lumbar spine BMD, suggesting their potential as osteoporosis severity indicators. No such correlations were observed with bone marrow markers. When analyzing postmenopausal women separately, we obtained consistent results. CONCLUSIONS: Elevated ß-CTX and OC levels in osteoporotic peripheral blood highlight their diagnostic significance. Negative ß-CTX and OC-BMD correlations underscore their potential for assessing osteoporosis severity. Discrepancies between peripheral blood and bone marrow markers emphasize the need for further exploration. This research advances our understanding of BTM clinical applications in osteoporosis diagnosis and treatment.
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Médula Ósea , Osteoporosis , Humanos , Femenino , Médula Ósea/diagnóstico por imagen , Procolágeno , Biomarcadores , Osteoporosis/diagnóstico por imagen , Remodelación Ósea , OsteocalcinaRESUMEN
Endometrial cancer (EC) is a common form of cancer in women. Metastasis is the main cause of EC treatment failure. Eukaryotic translation initiation factor 4E (eIF4E) is an oncogene that is overexpressed in a variety of malignancies and their distant metastases. The present study analyzed microarray data from the Oncomine database and revealed that high eIF4E expression was associated with poor prognosis and high pathological grade of EC. The expression of eIF4E was higher in EC tissues compared with in adjacent normal tissues. In addition, microRNA (miR)320a and miR3405p expression levels were downregulated in EC tissues compared with those in adjacent normal tissues, which suggested that these microRNAs may serve as EC tumor suppressor genes. miR320a and miR3405p could bind to the 3'UTR of eIF4E mRNA, thus downregulating the expression of eIF4E and phosphorylated (p)eIF4E in EC cells. Overexpression of miR320a or miR3405p effectively suppressed HEC1A cell migration and invasion. The downregulation of eIF4E and peIF4E following miR320a or miR3405p transfection reduced the invasiveness and metastatic capability of EC cells in a manner associated with decreased expression of matrix metallopeptidase (MMP)3 and MMP9. In addition, one of the effects of transforming growth factor ß1 (TGFß1), which is to induce the phosphorylation of eIF4E, was suppressed by miR320a and miR3405p overexpression. These two microRNAs also attenuated the features of TGFß1induced epithelialmesenchymal transition (EMT). In conclusion, the results of the present study demonstrated that eIF4E was upregulated in EC, whereas miR320a and miR3405p were downregulated in EC compared with adjacent normal tissues. In vitro, miR320a and miR3405p inhibited the migratory capability of EC cells by downregulating MMP3 and MMP9 and prevented TGFß1induced EMT through peIF4E.
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Neoplasias Endometriales/patología , Factor 4E Eucariótico de Iniciación/genética , MicroARNs/genética , Línea Celular Tumoral , Neoplasias Endometriales/genética , Neoplasias Endometriales/metabolismo , Transición Epitelial-Mesenquimal , Factor 4E Eucariótico de Iniciación/metabolismo , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Clasificación del Tumor , Metástasis de la Neoplasia , Pronóstico , Análisis de Supervivencia , Factor de Crecimiento Transformador beta1/metabolismoRESUMEN
Olinked ßNacetylglucosamine (OGlcNAc) modification is a dynamic posttranslational modification process that is involved in many crucial biological processes, including cell cycle regulation, nutrient metabolism and extracellular signaling. This dynamic modification is dependent on the ambient glucose concentration and is catalyzed and removed by OGlcNAc transferase (OGT) and OGlcNAcase (OGA), respectively. The present study aimed to determine the role of OGlcNAcylation during embryo implantation by inhibiting or enhancing its function and expression. The results revealed that the expression of OGlcNAcmodified proteins in the human secretory endometrium was higher than that of the endometrium during the proliferative phase, as determined via western blotting and immunohistochemistry. Additionally, the level of endometrial OGlcNAc modification increased gradually from the prereceptive to the receptive phase, which was then decreased during the nonreceptive phase. In endometrial cells, RNA interference was utilized to reduce the expression of two key OGlcNAc synthesis and decomposition enzymes, OGT and OGA, to indirectly increase or decrease levels of OGlcNAc modification. The results revealed that increasing the level of OGlcNAc modification enhanced cellular proliferation, migration, invasion and adhesion, thereby promoting embryo implantation. It is hypothesized that OGlcNAc modification serves an important role in the regulation of endometrial receptivity and embryo implantation. The results of the present study may have important implications for the understanding of female fertility and may help improve infertility treatments.