Genome-wide analysis reveals the contributors to fast molecular evolution of the Chinese hook snout carp (Opsariichthys bidens).

Variations in molecular evolutionary rate have been widely investigated among lineages and genes. However, it remains an open question whether fast rate of molecular evolution is driven by natural selection or random drift, and how the fast rate is linked to metabolic rate. Additionally, previous studies on fast molecular evolution have been largely restricted to concatenated matrix of genes or a few specifically selected genes, but less is known for individual genes at the genome-wide level. Here we addressed these questions using more than 5000 single-copy orthologous (SCO) genes through comparative genomic and phylogenetic analyses among fishes, with a special focus on a newly-sequenced clupeocephalan fish the Chinese hook snout carp Opsariichthys bidens. We showed O. bidens displays significantly higher mean substitution rate and more fast-evolving SCO genes (2172 genes) than most fishes studied here. The rapidly evolving genes are enriched in highly conserved and very basic functions such as translation and ribosome that are critical for biological fitness. We further revealed that ∼25 % of these fast-evolving genes exhibit a constant increase of substitution rate from the common ancestor down to the present, suggesting a neglected but important contribution from ancestral states. Model fitting showed that ∼85 % of fast-evolving genes exclusive to O. bidens and related species follow the adaptive evolutionary model rather than random-drift model, and 7.6 % of fast-evolving genes identified in O. bidens have experienced positive selection, both indicating the reflection of adaptive selection. Finally, metabolic rate was observed to be linked with substitution rate in a gene-specific manner. Overall, our findings reveal fast molecular evolution of SCO genes at genome-wide level in O. bidens, and uncover the evolutionary and ecological contributors to it.

Facile design of FeCu metal-organic frameworks anchored on layer Ti3C2Tx MXene for high-performance electrochemical sensing of resorcinol.

This work reports the rational design of a composite material by growing FeCu-MOF-919 on the surface of layered Ti3C2Tx MXene. The introduction of Ti3C2Tx MXene simultaneously weakens the aggregation of FeCu-MOF-919 and Ti3C2Tx MXene, which increases the electrochemical reaction active site of the composite material and improves the electrochemical activity. Interestingly, the FeCu-MOF-919/Ti3C2Tx based sensors were used to detect resorcinol (RS) with a wide linear range (0.5-152.5 µM), excellent sensitivity (0.23 µA µM-1 cm-2), low limit of detection (LOD = 0.08 µM) and outstanding stability. Meanwhile, the sensor shows high repeatability of 1.07 % RSD, reproducibility of 1.47 % RSD and anti-interference performance. What's more, the sensor can be successfully used to detect RS in tap water with good recoveries (96.25-103.37 %, RSD ≤2.18 %), demonstrating that the FeCu-MOF-919/Ti3C2Tx exhibits significant potential as an advanced sensing apparatus for the surveillance of RS in the natural environment.

Interface Engineering-Modulated Nanoscale Bimetallic CoFe-MIL-88A In-Situ-Grown on 2D V2CTx MXene for Electrocatalytic Nitrogen Reduction.

The electrochemical nitrogen reduction reaction (eNRR) provides a sustainable green development route for the nitrogen-neutral cycle. In this work, bimetallic CoFe-MIL-88A with two active sites (Fe, Co) were immobilized on a 2D V2CTx MXene surface by in situ growth method to achieve the purpose of the control interface. A large number of heterostructures are formed between small CoFe-MIL-88A and V2CTx, which regulate the electron transfer between the catalyst interfaces. The adsorption and activation of nitrogen on the active sites were enhanced, and the NRR reaction kinetics was accelerated. CoFe-MIL-88A is tightly arranged on V2CTx, which makes CoFe-MIL-88A/V2CTx have better hydrophobicity and can significantly inhibit the hydrogen evolution reaction. The synergistic effect of multicatalytic active sites and multi-interface structure of CoFe-MIL-88A/V2CTx MXene is propitious to nitrogen efficiently and stably to convert into ammonia under environmental conditions with superior selectivity and good catalytic activity. The NH3 yield rate is 29.47 µg h-1 mgcat-1 at -0.3 V vs RHE, and the Faradaic efficiency (FE) is 28.86% at -0.1 V vs RHE. The catalytic mechanism was verified to conform to the distal pathway. This work will provide a new way to develop an MXene-based electrocatalyst for eNRR.

In-situ synthesized and induced vertical growth of cobalt vanadium layered double hydroxide on few-layered V2CTx MXene for high energy density supercapacitors.

Two-dimensional (2D) MXene nanomaterials display great potential for green energy storage. However, as a result of self-stacking of MXene nanosheets and the presence of conventional binders, MXene-based nanomaterials are significantly hindered in their rate capability and cycling stability. We successfully constructed a self-supported stereo-structured composite (TMA-V2CTx/CoV-LDH/NF) by in-situ growing 2D cobalt vanadium layered double hydroxide (CoV-LDH) vertically on 2D few-layered V2CTx MXene nanosheets and interconnecting it with Ni foam (NF) with a self-supported structure to act as a binder-free electrode. In addition to inhibiting CoV-LDH aggregation, the highly conductive V2CTx MXene and CoV-LDH work synergistically to improve charge storage. The specific capacitance of the TMA-V2CTx/CoV-LDH/NF electrode is 2374 F/g (1187 C/g) at 1 A/g. At the same time, the TMA-V2CTx/CoV-LDH/NF exhibits excellent stability, retaining 85.3 % of its specific capacitance at 20 A/g after 10,000 cycles. In addition, the hybrid supercapacitor (HSC) is assembled based on positive electrode (TMA-V2CTx/CoV-LDH/NF) and negative electrode (AC), achieving the maximum energy density of 74.4 Wh kg-1 at 750.3 W kg-1. TMA-V2CTx/CoV-LDH/NF has potential as an electrode material for storing green energy. The research strategy provides a development prospect for the construction of novel V2CTx MXene-based electrode material with self-supported structures.

Manganese-cobalt hydroxide nanosheets anchored on a hollow sulfur-doped bimetallic MOF for high-performance supercapacitors and the hydrogen evolution reaction in alkaline media.

Nonmetallic doping and in situ growth techniques for designing electrode materials with excellent electrocatalytic activity are effective strategies to enhance the electrochemical performance. Bifunctional electrode materials for supercapacitors (SCs) and the hydrogen evolution reaction (HER) have attracted great interest due to their potential applications in green energy storage and conversion. Herein, the bimetallic MnCo LDH is anchored on a hollow sulfur (S)-doped MnCo-MOF-74 surface, forming a poplar flower-like 3D composite which is used for SCs and the HER in alkaline media. The fabricated S-MnCo-MOF-74@MnCo LDH/NF electrode exhibits a favorable specific capacitance of 1875.4 F g-1 at 1 A g-1 and steady long-term cycling performance. Moreover, the assembled HSC using S-MnCo-MOF-7@MnCo LDH/NF as the cathode material and active carbon (AC) as the anode material shows 546.4 F g-1 capacitance (1 A g-1) with a maximum energy density of 58 W h kg-1 at 14 000 W kg-1 power density. As an electrocatalyst, S-MnCo-MOF-7@MnCo LDH/NF exhibits excellent HER properties with a small Tafel slope of 128.9 mV dec-1 a low overpotential of 197 mV at 10 mA cm-2 and durable performance for 10 hours in alkaline media. The present work provides insights into understanding and designing active electrode materials for stable hydrogen evolution and high-performing supercapacitors in an alkaline environment.

Thyroid-stimulating hormone may participate in insulin resistance by activating toll-like receptor 4 in liver tissues of subclinical hypothyroid rats.

BACKGROUND: Thyroid-stimulating hormone (TSH) is an independent risk factor of and closely associated with metabolic disorders. In the present study, we explored the potential mechanism and adverse effects of TSH on insulin resistance in the liver of subclinical hypothyroidism models in vivo. METHODS: The mean glucose infusion rate (GIR), free fatty acids (FFAs), the homeostatic model assessment for insulin resistance (HOMA-IR), fasting plasma insulin (FINS), the TLR4 signal pathway and its intracellular negative regulator-toll-interacting protein (Tollip), and the modulators of insulin signaling were evaluated. RESULTS: Compared to the normal control group (NC group), the subclinical hypothyroidism rat group (SCH group) showed decreases in GIR and increases in FFAs, FINS, and HOMA-IR. The levels of TLR4 and of its downstream molecules like p-NF-κB, p-IRAK-1, IL-6 and TNF-α were evidently higher in the SCH group than in the NC group. Conversely, the level of Tollip was significantly lower in the SCH group than in the NC group. Compared to the NC group, the levels of phosphorylated IRS-1-Tyr and GLUT2 were decreased in the SCH group. Macrophage infiltration was higher in the SCH group than in the NC group. CONCLUSION: TSH may participate in aggravating inflammation by increasing macrophage infiltration; furthermore, it may activate the TLR4-associated inflammatory signaling pathway, thus interfering with insulin signals in liver tissues. Targeting TSH may have therapeutic benefits against metabolic disorders.

Higher Food Yields and Lower Greenhouse Gas Emissions from Aquaculture Ponds with High-Stalk Rice Planted.

Aquaculture ponds are an important artificial aquatic system for global food fish production but also are a hot spot of greenhouse gas (GHG) emissions. The GHG mitigation strategy and the underlying mechanism for aquaculture ponds are still poorly understood. In this study, we conducted a 2 year field experiment to determine the effects of planting high-stalk rice (an artificially bred emergent plant for ponds) on GHG emissions from aquaculture ponds. Our results showed that planting high-stalk rice reduced CH4 emission by 64.4% and N2O emission by 76.2% over 2 years. Planting high-stalk rice significantly increased the content of O2 and the abundance of pmoA in the sediment, thus prompting CH4 oxidation in the ponds. The reduction of N2O emission from ponds was attributed to the decreased inorganic nitrogen, amoA-B and nirS in the sediment induced by rice. Furthermore, high-stalk rice culture in the pond increased shrimp yields and gained rice yields, resulting in a significant reduction of yield-scaled global warming potential. Our findings suggest that breeding appropriate emergent aquatic plants is a potential pathway to mitigate GHG emission from aquaculture ponds with more food yields and economic benefits.

MIL-101(Cr) molecular cage anchored on 2D Ti3C2TX MXene nanosheets as high-performance electrochemical sensing platform for detection of xanthine.

A new electrochemical sensing material based on the MIL-101(Cr) molecular cage anchored on 2D Ti3C2TX-MXene nanosheets was prepared by using the in situ growth molecular engineering strategy. The sensing material was characterized by using different methods such as SEM, XRD, and XPS. The electrochemical sensing performance of MIL-101(Cr)/Ti3C2Tx-MXene was studied by DPV, CV, EIS, and other techniques. The electrochemical tests showed that the linear range of the modified electrode for xanthine (XA) detection was 1.5-73.0 µM and 73.0-133.0 µM, the detection limit was 0.45 µM (working potential of + 0.71 V vs. Ag/AgCl), and the performance is superior compared with the reported enzyme-free modified electrodes for detecting XA. The fabricated sensor has high selectivity and stability. It has good practicability in serum analysis with recoveries of 96.58-103.27% and a relative standard deviation (RSD) of 3.58-4.32%.

Development zone policy and urban carbon emissions: empirical evidence from the construction of national high-tech industrial development zones in China.

As a key strategy to promote system reform, improve the investment environment, and encourage industrial agglomeration, the national high-tech industrial development zone (NHTDZ) policy in China can not only reduce energy consumption through the scale effect but also improve energy efficiency by modernizing industrial structure and fostering technological innovation, thereby alleviating environmental pollution. Existing studies, however, focus solely on the effects of NHTDZ policy on social and economic development, ignoring their impact on the ecological environment, especially carbon (CO2) emissions that contribute to global warming. Thus, this article analyzes a panel data of 285 prefecture-level cities and above in China from 2003 to 2019 to assess the influence of NHTDZ policy on CO2 emissions, treating the NHTDZ construction since 1988 as a quasi-natural experiment. The results indicate that the NHTDZ policy would mitigate urban carbon emissions, particularly in middle, southeastern, medium-sized, resource-based (RB), non-key environmental protection (non-KEP), and non-two control zone (non-TCZ) cities. In addition, the mediation mechanism test demonstrates that the environmental benefits of the NHTDZ policy in China are attributable to the scale effect, the structural upgrading effect, and the technology innovation effect. The NHTDZ policy would lower per capita CO2 emissions by reducing energy consumption, upgrading industrial structure, and promoting green technology innovation.

In vitro Anti-malignant Property of PCMT1 Silencing and Identification of the SNHG16/miR-195/PCMT1 Regulatory Axis in Breast Cancer Cells.

BACKGROUND: Protein L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1) is a highly conserved protein repair enzyme that participates in regulating the progression of human cancers. We therefore studied the function and the related mechanisms of PCMT1 in breast cancer cells. METHODS: Expression profile and prognostic analysis of PCMT1 in breast cancer patients were analyzed using online databases. PCMT1 expression in breast cancer cells was detected by western blot analysis. Cell proliferation was determined by CCK-8 and colony formation assays. Apoptosis was evaluated using flow cytometry analysis and caspase-3/7 activity assay. Cell invasion was assessed by Transwell invasion assay. The small nucleolar RNA host gene 16 (SNHG16)/miR-195/PCMT1 regulatory axis was identified using bioinformatics analysis. RESULTS: PCMT1 expression was increased in breast cancer tissues and cells. High PCMT1 expression was correlated with poor prognosis in breast cancer patients. PCMT1 knockdown suppressed cell proliferation and colony formation ability in breast cancer cells. Moreover, PCMT1 knockdown induced apoptosis and restrained the invasive ability in breast cancer cells. PCMT1 overexpression increased the proliferative and invasive abilities of breast cancer cells. miR-195 was identified as the unique upstream miRNA of PCMT1. SNHG16 was identified as the unique upstream lncRNA of miR-195. SNHG16 knockdown downregulated PCMT1 by increasing miR-195 expression. Breast cancer cell proliferation was regulated by the SNHG16/miR-195/PCMT1 axis. CONCLUSION: PCMT1 silencing inhibited cell proliferation and invasion and induced apoptosis in breast cancer cells and the SNHG16/miR-195/PCMT1 regulatory axis might serve as a potential therapeutic target for breast cancer.

In situ growth of ZIF-67-derived nickel-cobalt-manganese hydroxides on 2D V2CTx MXene for dual-functional orientation as high-performance asymmetric supercapacitor and electrochemical hydroquinone sensor.

Designing dual-functional electrode materials for supercapacitors and pollutant sensors has attracted great interest from researchers for urgent demand in green energy and the environment. In this work, a novel electrode material V2CTx@NiCoMn-OH was successfully constructed for dual-functional orientation via a two-step synthesis strategy, in which the NiCoMn-OH with a three-dimensional (3D) hollow structure was fabricated by employing ZIF-67 as a template and simple anion exchange and composited with the two-dimensional (2D) layered V2CTx MXene. The intercalation of NiCoMn-OH can effectively limit the self-accumulation of V2CTx MXene nanosheets and build a 3D cross-linked hollow structure, thereby broadening the ion transport channel, exposing more active sites of V2CTx@NiCoMn-OH, and simultaneously improving the conductivity of NiCoMn-OH. Benefiting from the unique 3D cross-linked hollow structure, the optimized V2CTx@NiCoMn-OH-20 electrode material exhibits an excellent specific capacitance of 827.45 C g-1 at 1 A g-1. Furthermore, the electrode material has excellent capacitance retention of 88.44% after 10,000 cycles. Moreover, the V2CTx@NiCoMn-OH-20//AC ASC device displays a high energy density of 88.35 Wh kg-1 as well as high power density of 7500 W kg-1 during operation. Additionally, the V2CTx@NiCoMn-OH-20 exhibited excellent electrocatalytic performance in the detection of hydroquinone, including the low detection limit of 0.559 µM (S/N = 3) and the wide linear range of 2-1050 µM. Therefore, the prepared V2CTx@NiCoMn-OH-20 has great potential applications in the fields of supercapacitors and hydroquinone sensors.

A sensing platform based on Cu-MOF encapsulated Dawson-type polyoxometalate crystal material for electrochemical detection of xanthine.

A promising sensing platform based on polyoxometalate-based metal-organic framework (POMOF) was established for sensitive electrochemical detection of xanthine (XA). In the unique structure of POMOF, the Dawson polyoxoanions P2W18 were encapsulated into 3D open copper-mixed ligand nanotube framework Cu-MOF, in which the cavity of the metal-organic framework provides a specific shelter to prevent the aggregation and loss of polyoxometalate in electrocatalytic reactions; meanwhile, unsaturated Cu(II) active sites of Cu-MOF can also serve as electrocatalytic active center. The POMOF-based sensor (CuMOFP2W18/XC-72R) was fabricated by using acetylene black (XC-72R) as a support material to enhance the conductivity of POMOF. The performances of the POMOF-based sensor were studied by using different electrochemical testing methods. The composite displayed remarkable electrocatalytic activity for the oxidation of XA due to the synergistic effect of polyoxometalate (POM) and metal-organic framework (MOF). The electrochemical sensor demonstrated a wide linear range (0.5 µM-240 µM), low detection limit (0.26 µM), and excellent selectivity for detecting XA. Furthermore, the composite further demonstrated excellent reproducibility and great stability. More importantly, the proposed sensor was utilized to detect XA in real samples, which may provide a new way for early disease diagnosis.

Eimeria spp. (Eimeriidae) in the migratory whooper swan (Cygnus cygnus) Linnaeus, 1758 (Anatidae) from Sanmenxia Swan Lake National Urban Wetland Park in the middle reaches of the Yellow River in China.

This study i dentifies four Eimeria spp. recorded from fecal samples of migratory whooper swans (Cygnus cygnus) in Sanmenxia Swan Lake National Urban Wetland Park in Sanmenxia city in the middle reaches of the Yellow River, China. Eimeria hermani, Eimeria nocens, Eimeria stigmosa, and Eimeria magnalabia were compatible in all characteristic features with their respective original descriptions. In addition to the preliminary morphological identification, this study provides a preliminary genotypic identification of these four Eimeria spp. via sequencing of the 18S rRNA, 28S rRNA, and COI gene loci that are suitable for the genotypic differentiation of these coccidia. This is the first report of molecular data for the four Eimeria spp. in migratory whooper swans. Finally, this study discusses the environmental risks of these coccidia for migratory whooper swans in Sanmenxia Swan Lake National Urban Wetland Park.

The effects of hsa_circ_0000517/miR-326 axis on the progression of breast cancer cells and the prediction of miR-326 downstream targets in breast cancer.

It has been proposed that circular RNAs (circRNAs) play crucial roles in the initiation and progression of various cancers including breast cancer. Our study aimed to determine the function and regulatory mechanism of hsa_circ_0000517 in breast cancer. qRT-PCR was applied to determine hsa_circ_0000517 expression in breast cancer cells. The circular structure of hsa_circ_0000517 was confirmed using RNase R digestion assay. The subcellular distribution of hsa_circ_0000517 was analyzed using nuclear mass separation assay. Effects of hsa_circ_0000517 on the malignant behaviors of breast cancer cells were determined using CCK-8, colony formation assay, flow cytometry analysis, caspase-3 activity assay, and Transwell invasion assay. Bioinformatics analysis, luciferase reporter assay, and RIP were used to predict and confirm the interaction between hsa_circ_0000517 and miR-326. Bioinformatics analysis was used to search the possible targets of miR-326. Hsa_circ_0000517 was upregulated in breast cancer tissues and cells. Hsa_circ_0000517 was a stable circularized transcript that was preferentially distributed in the cytoplasm. Hsa_circ_0000517 knockdown inhibited cell proliferation, colony formation ability, and invasion and triggered apoptosis in breast cancer cells. Hsa_circ_0000517 acted as a sponge of miR-326 to suppress its expression. miR-326 inhibition abolished the effects of hsa_circ_0000517 knockdown on the malignant behaviors of breast cancer cells. Totally 17 genes were identified as the potential targets of miR-326 in breast cancer. In conclusion, hsa_circ_0000517 silencing repressed breast cancer progression by upregulating miR-326 expression.

Effect of phosphorus and potassium addition on greenhouse gas emissions and nutrient utilization of a rice-fish co-culture system.

Greenhouse gas (GHG) emissions from aquaculture have gained widespread attention. However, the effect of phosphorus (P) and potassium (K) on GHG emissions from aquaculture systems has rarely been studied. In this study, we conducted a laboratory-scale experiment to investigate the effect of P and K addition on CH4 and N2O emissions and nutrient use efficiency in a rice-fish co-culture system. The results showed that the CH4 flux rate did not differ between the rice-fish co-culture (RF) and fish monoculture (F) systems. Phosphorus addition did not affect CH4 emission from the RF. In contrast, K addition significantly increased the CH4 emission from the RF by 148.4%. Dual P and K addition greatly increased the CH4 emission from the RF by six times, indicating an interactive effect of P and K on the stimulation of CH4 emission. Phosphorus addition strengthened the restorative effect of the RF on N2O emission, while K addition weakened the restorative effect of the RF on N2O emission. The combination of P and K did not affect the N2O emission from the RF. The application of P and K strengthened the restorative effect of rice on nitrogen (N) pollution in aquaculture water. Phosphorus and K addition significantly increased the rice biomass and nutrient in the harvested rice, but did not affect the fish biomass and nutrient in the harvested fish. Dual P and K addition increased the nutrient use efficiency in the rice-fish system. These results provide a reference for adjusting nutrient management to reduce GHG emissions and improve nutrient use efficiency in the rice-fish system.

ROR1-AS1 knockdown inhibits growth and invasion and promotes apoptosis in NSCLC cells by suppression of the PI3K/Akt/mTOR pathway.

The role of ROR1-AS1 in non-small-cell lung cancer (NSCLC) remains unclear. Therefore, we aimed to investigate the functional role of ROR1-AS1 in NSCLC and to explore the underlying mechanisms. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was performed to detect cell proliferation. Transwell assay was performed to evaluate cell invasive ability. Cell apoptotic rates and caspase-3/7 activity were determined to evaluate apoptosis. The expression levels of PI3K/Akt/mTOR pathway-related proteins were measured using Western blot analysis. Results showed that ROR1-AS1 expression was upregulated in NSCLC samples. Knockdown of ROR1-AS1 inhibited the viability and invasive ability of NSCLC cells. Knockdown of ROR1-AS1 induced apoptotic rate and caspase-3/7 activity and suppressed xenograft NSCLC tumor growth. In addition, ROR1-AS1 knockdown inhibited the activation of the PI3K/Akt/mTOR pathway in NSCLC cells. However, treatment with 740Y-P prevented the effects of si-ROR1-AS1 on viability, invasive ability, and apoptosis of NSCLC cells. These findings implied that ROR1-AS1 played an oncogenic role in NSCLC via regulating the PI3K/Akt/mTOR pathway.

Bimetallic FeNi-MIL-88-derived NiFe2O4@Ni-Mn LDH composite electrode material for a high performance asymmetric supercapacitor.

A simple continuous hydrothermal method was used to synthesize a NiFe2O4@Ni-Mn LDH/NF composite. The layered structure provides a large void to transfer the electron effectively, and the composite materials exhibit remarkable electrochemical performance including excellent specific capacitance (1265 F g-1 at 1 A g-1) and remarkable cycling stability (the specific capacitance remains at 80.9% after 5000 cycles). In addition, the asymmetric supercapacitor exhibits a high energy density of 96.2 W h kg-1 at a power density of 700 W kg-1, and there is an extraordinarily good cycling stability with a capacity retention rate of 92.5% after 4000 cycles. The outcomes indicate that the NiFe2O4@Ni-Mn LDH/NF composite electrode has potential application as a high-performance supercapacitor.

AOC4P suppresses viability and invasion and induces apoptosis in NSCLC cells by inhibiting the Wnt/ß-catenin pathway.

Increasing studies have well-documented the involvement of numerous lncRNAs in regulating the malignant phenotypes of various tumors including non-small cell lung cancer (NSCLC) cells. However, up to date, the effects and mechanism of lncRNA amine oxidase, copper containing 4, pseudogene (AOC4P) in NSCLC progression remain undefined. AOC4P expression in NSCLC cells was detected by qRT-PCR. The protein levels of Wnt/ß-catenin pathway-related proteins, matrix metallopeptidase (MMP)-2, and MMP-9 were examined by Western blot. The effects of AOC4P or combined with Wnt agonist BML-284 on the malignant phenotypes in NSCLC cells were explored by CCK-8, Transwell invasion assay, flow cytometry analysis and caspase-3/7 activity. AOC4P was lowly expressed in NSCLC samples and cells. Overexpression of AOC4P inhibited viability, the expression of MMP-2 and MMP-9, and invasion of NSCLC cells. Apoptosis and caspase-3/7 activity were suppressed in response to AOC4P overexpression in NSCLC cells. AOC4P overexpression suppressed tumor growth in a xenograft mouse model. Activation of the Wnt/ß-catenin pathway by BML-284 abolished the effects of AOC4P overexpression on cell viability, invasion and apoptosis in NSCLC cells. In conclusion, AOC4P overexpression suppresses viability and invasion and induces apoptosis in NSCLC cells via inhibition of the Wnt/ß-catenin pathway.

A novel dual-tasking hollow cube NiFe2O4-NiCo-LDH@rGO hierarchical material for high preformance supercapacitor and glucose sensor.

Binary transition metal oxides as electroactive materials have continuously aroused grumous attention due to their high theoretical specific capacitance, high valtage window, and multiple oxidation states. However, the tiny specific surface area, poor conductivity and unsatisfactory cycle stability limit their practical application. Hence, a synthetic strategy is designed to fabricate a dual-tasking hollow cube nickel ferrite (NiFe2O4) - based composite (NiFe2O4-NiCo-LDH@rGO) with hierarchical structure. The composite is constructed by firstly preparing hollow NiFe2O4 from cube-like Ni - Fe bimetallic organic framework (NiFe-MOF), and then integrating nickel cobalt layered double hydroxide (NiCo-LDH) nanowires, together with reduced graphene oxide (rGO) via pyrolysis in conjuction with hydrothermal method. The NiFe2O4 possessing cubic hollow structure contributes to a huge accessible surface area, meanwhile alleviates large volume expansion/contraction effect, which facilitates suffcient permeation of the electrolyte and rapid ion/charge transport, and results in high cycling stability. The introduction of layered NiCo-LDH results in hierarchical structure and thus offers maximum contact areas with electrolyte, which heightens the specific capacitance of obtained composite and enhances the electro-catlytic activity towards oxidation of glucose. Furthermore, rGO layer greatly improves the electrical conductivity and ion diffusion/transport capability of composite. Benefiting from the unique structure and individual components of NiFe2O4-NiCo-LDH@rGO composite, the electrode delivers a high specific capacitance (750 C g-1) and superb durability. Simultaneously, the asymmetrical device based on NiFe2O4-NiCo-LDH@rGO as positive electrode delivers remarkable energy density (50 Wh kg-1). Moreover, NiFe2O4-NiCo-LDH@rGO exhibits good sensing performance with a sensitivity of 111.86 µA/µM cm-2, the wide linear range of 3.500 × 10-5 - 4.525 × 10-3 M, and the detection limit of 12.94 × 10-6 M with a signal to noise ratio of 3. Consequently, the NiFe2O4-NiCo-LDH@rGO could provide a prospective notion constructing bifunctional materials with hollow-cube hierarchical structure in the field of supercapacitors and electrochemical sensors.