Toward Citizen Science-Based Ocean Acidification Observations Using Smartphone Devices.

pH is a key parameter in many chemical, biological, and biogeochemical processes, making it a fundamental aspect of environmental monitoring. Rapid and accurate seawater pH measurements are essential for effective ocean observation and acidification investigations, resulting in the need for novel solutions that allow robust, precise, and affordable pH monitoring. In this study, a versatile smartphone-based environmental analyzer (vSEA) was used for the rapid measurement of seawater pH in a field study. The feasibility of the use of the vSEA algorithm for pH quantification was explored and verified. When used in conjunction with a three-dimensional (3D)-printed light-proof shell, the quality of captured images is guaranteed. The quantitative accuracy of vSEA pH measurements reached 0.018 units with an uncertainty of <0.01, meeting the requirements of the Global Ocean Acidification Observing Network (GOA-ON) for "weather" goals (permitting a maximum pH uncertainty of 0.02). The vSEA-pH system was successfully applied for on-site pH measurements in coastal seawater and coral systems. The performance of the vSEA-pH system was validated using different real-world samples, and t-test results showed that the vSEA-pH system was consistent with pH measurements obtained using a state-of-the-art benchtop spectrophotometer (t = 1.986, p = 0.7949). The vSEA-pH system is applicable to different types of smartphone devices, making it possible for vSEA-pH to be widely promoted for public citizen use. The vSEA-pH system offers a simple, accurate, and applicable method for the on-site measurement of seawater pH, assisting the large-scale monitoring of ocean acidification by allowing the contribution of citizen science-based data collection.

Development of a versatile smartphone-based environmental analyzer (vSEA) and its application in on-site nutrient detection.

The citizen-science-based environmental survey can benefit from the smartphone technology used in chemical and biological sensing of a wide range of analytes. Quantification by smartphone-based colorimetric assays is being increasingly reported, however, most of the quantification uses empirical formula or complex exhaustive methods. In this study, a versatile and robust algorithm is proposed to overcome these limitations. A model is established to simulate and analyze the conversion process from the camera's spectral information into RGB (Red, Green, Blue) color information. Moreover, the feasibility of the algorithm for the quantification of different analytes is also explored. Based on this algorithm, a versatile smartphone-based environmental analyzer (vSEA) is built and its reliability, versatility, and analytical performance are comprehensively optimized. The good linearity (R2 ≥ 0.9954) and precision (relative standard deviations < 5.3%) indicates that the vSEA is accurate enough to quantify the nutrients in most natural waters. Furthermore, the vSEA is used for the field measurement of five important nutrients, and the results show no significant difference compared to conventional methods. The vSEA offers a simpler and easier method for the on-site measurement of nutrients in natural water bodies, which can aid in the emergency monitoring of aqueous ecosystems and the performance of citizen-science-based research.

Towards citizen science. On-site detection of nitrite and ammonium using a smartphone and social media software.

Citizen scientists-based water quality surveys are becoming popular because of their wide applications in environmental monitoring and public education. At present, many similar studies are reported on collecting samples for later laboratory analysis. For environmentally toxic analytes such as ammonium and nitrite, on-site detection is a promising choice. However, this approach is limited by the availability of suitable methods and instruments. Here, a simple on-site detection method for ammonium and nitrite is reported. The chemistry of this method is based on the classic Griess reaction and modified indophenol blue reaction. Digital image colorimetry is carried out using a smartphone with a custom-made WeChat mini-program or free built-in applications (APPs). Using a simple and low-cost analytical kit, the detection limit of 0.27 µmol/L and 0.84 µmol/L is achieved for nitrite and ammonium, respectively, which are comparable to those achieved with a benchtop spectrophotometer. Relative standard deviations (n = 7) for low and high concentrations of nitrite are 3.6% and 4.3% and for ammonium are 5.6% and 2.6%, respectively. Identical results with a relative error of less than 10% are obtained using different smartphones (n = 3), color extracting software (n = 6), and with multiple individual users (n = 5). These results show the robustness and applicability of the proposed method. The on-site application is carried out in an in-campus wastewater treatment plant and at a local river. A total of 40 samples are analyzed and the analytical results are compared with that obtained by a standard method and a spectrophotometer, followed by a paired t-test at a 95% confidence level. This proposed on-site analytical kit has the advantages of simplicity and portability and has the potential to be popular and useful for citizen science-based environmental monitoring.