RESUMEN
In recent years, the influx of business capital to rural areas, land transfer and adjustment in planting structure have led to the widespread of "non-grain production" of cultivated land in China, which threatens the "1.8 billion mu of arable land protection red line" as well as national food security. Both tillage layer stripped and unstripped are examples of "non-grain production" of cultivated land, which are detrimental to long-term food security because they might reduce soil fertility to varied degrees. In the former case, the original topsoil has been destroyed and the tillage layer is gone. In the latter, there may be impediments such as acidification and salinization. Domestic and international scholars have conducted extensive research on the improvement of degraded soils, including measures with guest soil and soil replacement, the reduction of soil barrier factors, biological fertilization and other measures. There are no systematic research results on the remediation of "non-grain production" of cultivated land. Using data from the National Statistical Yearbook data and literature analysis, we systematically summarized current status of "non-grain production" of cultivated land and key technologies for land improvement, recultivation and fertilization in China, and put forward future directions in this area.
Asunto(s)
Grano Comestible , Suelo , China , Tecnología , FertilizaciónRESUMEN
To elucidate the physiological and morphological responses generated by vanadium (V) in plants, hydroponic culture experiments were performed with swamp morning glory (Ipomoea aquatica Forsk) exposed to 0 mg L(-1) to 2.50 mg L(-1) pentavalent V [V(V)] in Hoagland nutrient solutions. The concentration of chlorophyll a, chlorophyll b, and carotene peaked at a V(V) concentration of 0.05 mg L(-1) and gradually decreased at higher V(V) concentrations. Similarly, the plant biomass was stimulated at low levels of V(V) and was inhibited when V(V) concentrations exceeded 0.1 mg L(-1). Pentavalent V had negative effects on the uptake of phosphorus (P) by roots, shoots, and leaves. The biological absorption coefficients of V of the roots were higher than those of the aerial parts. Under low concentrations of V(V) exposure, the predominant species of V in the aerial parts was tetravalent V [V(IV)], whereas V(V) became more prevalent when concentrations of V(V) in the solution was higher than 0.50 mg L(-1). In the roots, however, the concentrations of V(V) were always higher than those of the V(IV), except in the control group. Organelles in the V(V)-treated leaves were distorted, and the periplasmic space became wider. These results indicate V(V) has concentration-dependent effects on the physiological properties of swamp morning glory, whereas the plant has the ability to develop self-protective function to adapt to the toxicity of V(V).
Asunto(s)
Ipomoea/efectos de los fármacos , Vanadio/toxicidad , Biomasa , Carotenoides/metabolismo , Clorofila/metabolismo , Clorofila A , Hidroponía , Ipomoea/crecimiento & desarrollo , Ipomoea/metabolismo , Fósforo/metabolismo , Fotosíntesis/efectos de los fármacos , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Raíces de Plantas/química , Raíces de Plantas/metabolismo , Brotes de la Planta/química , Brotes de la Planta/metabolismo , Vanadio/metabolismoRESUMEN
A microcosm experiment was conducted to investigate the dissipation of available benzo[a]pyrene (BaP) in soils co-contaminated with cadmium (Cd) and pyrene (PYR) during aging process. The available residue of BaP in soil was separated into desorbing and non-desorbing fractions. The desorbing fraction contributed more to the dissipation of available BaP than the non-desorbing fraction did. The concentration of bound-residue fraction of BaP was quite low across all treatments. Within the duration of this study (250 days), transformation of BaP from available fractions to bound-residue fraction was not observed. Microbial degradation was the dominant mechanism of the dissipation of available BaP in the soil. The dissipation of available BaP was significantly inhibited with the increment in Cd level in the soil. The addition of PYR (250 mg kg(-1)) remarkably promoted the dissipation of available BaP without reducing Cd availability in the soil. The calculated half-life of available BaP in the soil prolonged with the increment in Cd level; however, the addition of PYR shortened the half-life of available BaP by 13.1, 12.7, and 32.8% in 0.44, 2.56, and 22 mg Cd kg(-1) soils, respectively. These results demonstrated that the inhibiting effect of Cd and the promoting effect of PYR on the dissipation of available BaP were competitive. Therefore, this study shows that the bioremediation process of BaP can be more complicated in co-contaminated soils.
Asunto(s)
Benzo(a)pireno/análisis , Cadmio/análisis , Contaminantes del Suelo/análisis , Benzo(a)pireno/química , Benzo(a)pireno/metabolismo , Biodegradación Ambiental , Cadmio/química , Cadmio/metabolismo , Semivida , Modelos Químicos , Suelo/química , Microbiología del Suelo , Contaminantes del Suelo/química , Contaminantes del Suelo/metabolismoRESUMEN
A pot culture experiment was carried out to investigate the accumulation properties of mercury (Hg) in rice grain and cabbage grown in seven soil types (Udic Ferrisols, Mollisol, Periudic Argosols, Latosol, Ustic Cambosols, Calcaric Regosols, and Stagnic Anthrosols) spiked with different concentrations of Hg (CK, 0.25, 0.50, 1.00, 2.00, and 4.00 mg/kg). The results of this study showed that Hg accumulation of plants was significantly affected by soil types. Hg concentration in both rice grain and cabbage increased with soil Hg concentrations, but this increase differed among the seven soils. The stepwise multiple regression analysis showed that pH, Mn(II), particle size distribution, and cation exchange capacity have a close relationship with Hg accumulation in plants, which suggested that physicochemical characteristics of soils can affect the Hg accumulation in rice grain and cabbage. Critical Hg concentrations in seven soils were identified for rice grain and cabbage based on the maximum safe level for daily intake of Hg, dietary habits of the population, and Hg accumulation in plants grown in different soil types. Soil Hg limits for rice grain in Udic Ferrisols, Mollisol, Periudic Argosols, Latosol, Ustic Cambosols, Calcaric Regosols, and Stagnic Anthrosols were 1.10, 2.00, 2.60, 2.78, 1.53, 0.63, and 2.17 mg/kg, respectively, and critical soil Hg levels for cabbage are 0.27, 1.35, 1.80, 1.70, 0.69, 1.68, and 2.60 mg/kg, respectively.
Asunto(s)
Brassica/química , Mercurio/análisis , Oryza/química , Contaminantes del Suelo/análisis , Suelo/química , China , Mercurio/química , Contaminantes del Suelo/químicaRESUMEN
The study on biochar derived from plant biomass for environmental applications is attracting more and more attention. Twelve sets of biochar were obtained by treating four phytoremediation plants, Salix rosthornii Seemen, Thalia dealbata, Vetiveria zizanioides, and Phragmites sp., sequentially through pyrolysis at 500 °C in a N2 environment, and under different temperatures (500, 600, and 700 °C) in a CO2 environment. The cation exchange capacity and specific surface area of biochar varied with both plant species and pyrolysis temperature. The magnesium (Mg) content of biochar derived from T. dealbata (TC) was obviously higher than that of the other plant biochars. This biochar also had the highest sorption capacity for phosphate and ammonium. In terms of biomass yields, adsorption capacity, and energy cost, T. dealbata biochar produced at 600 °C (TC600) is the most promising sorbent for removing contaminants (N and P) from aqueous solution. Therefore, T. dealbata appears to be the best candidate for phytoremediation application as its biomass can make a good biochar for environmental cleaning.
Asunto(s)
Compuestos de Amonio/aislamiento & purificación , Carbón Orgánico/química , Fosfatos/aislamiento & purificación , Extractos Vegetales/química , Ultrafiltración/métodos , Contaminantes Químicos del Agua/aislamiento & purificación , Purificación del Agua/métodos , Adsorción , Compuestos de Amonio/química , Biodegradación Ambiental , Fosfatos/química , Soluciones/análisis , Soluciones/química , Contaminantes Químicos del Agua/químicaRESUMEN
The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R(2)>0.95). The calculated maximum adsorption parameter (Q) of Cd was similar in single and binary systems (34129.69 and 35919.54 mg/kg, respectively). However, the Q of SMX in a binary system (9182.74 mg/kg) was much higher than that in a single system (1827.82 mg/kg). The presence of Cd significantly promoted the sorption of SMX on rice straw biochar. When the pH ranged from 3 to 7.5, the sorption of Cd had the characteristics of a parabola pattern with maximum adsorption at pH 5, while the adsorption quantity of SMX decreased with increasing pH, with maximum adsorption at pH 3. The amount of SMX adsorbed on biochar was positively correlated with the surface area of the biochar, and the maximum adsorption occurred with d 250 biochar (biochar with a diameter of 150-250 µm). Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) showed that the removal of Cd and SMX by rice straw biochar may be attributed to precipitation and the formation of surface complexes between Cd or SMX and carboxyl or hydroxyl groups. The results of this study indicate that rice straw biochar has the potential for simultaneous removal of Cd and SMX from co-contaminated water.
Asunto(s)
Cadmio/química , Cadmio/aislamiento & purificación , Sulfametoxazol/química , Sulfametoxazol/aislamiento & purificación , Purificación del Agua/métodos , Adsorción , Cromatografía Líquida de Alta Presión , Concentración de Iones de Hidrógeno , Microscopía Electrónica de Rastreo , Oryza , Soluciones/química , Espectroscopía Infrarroja por Transformada de Fourier , Contaminantes Químicos del Agua/aislamiento & purificaciónRESUMEN
The elucidation of a natural strategy for metal hyperaccumulation enables the rational design of technologies for the clean-up of metal-contaminated soils. Organic acid has been suggested to be involved in toxic metallic element tolerance, translocation, and accumulation in plants. The impact of exogenous organic acids on cadmium (Cd) uptake and translocation in the zinc (Zn)/Cd co-hyperaccumulator Sedum alfredii was investigated in the present study. By the addition of organic acids, short-term (2 h) root uptake of (109)Cd increased significantly, and higher (109)Cd contents in roots and shoots were noted 24 h after uptake, when compared to controls. About 85% of the (109)Cd taken up was distributed to the shoots in plants with citric acid (CA) treatments, as compared with 75% within controls. No such effect was observed for tartaric acid (TA). Reduced growth under Cd stress was significantly alleviated by low CA. Long-term application of the two organic acids both resulted in elevated Cd in plants, but the effects varied with exposure time and levels. The results imply that CA may be involved in the processes of Cd uptake, translocation and tolerance in S. alfredii, whereas the impact of TA is mainly on the root uptake of Cd.
Asunto(s)
Cadmio/aislamiento & purificación , Cadmio/farmacocinética , Ácido Cítrico/farmacología , Sedum/metabolismo , Contaminantes del Suelo/aislamiento & purificación , Contaminantes del Suelo/farmacocinética , Tartratos/farmacología , Biodegradación Ambiental , Tasa de Depuración Metabólica/efectos de los fármacos , Sedum/efectos de los fármacosRESUMEN
The development of an integrated strategy for the remediation of soil co-contaminated by heavy metals and persistent organic pollutants is a major research priority for the decontamination of soil slated for use in agricultural production. The objective of this study was to develop a bioremediation strategy for fields co-contaminated with cadmium (Cd), dichlorodiphenyltrichloroethane (DDT), and its metabolites 1, 1-dichloro-2, 2-bis (4-chlorophenyl) ethylene (DDE) and 1, 1-dichloro-2, 2-bis (4-chlorophenyl) ethane (DDD) (DDT, DDE, and DDD are collectively called DDs) using an identified Cd-hyperaccumulator plant Sedum alfredii (SA) and DDT-degrading microbes (DDT-1). Initially, inoculation with DDT-1 was shown to increase SA root biomass in a pot experiment. When SA was applied together with DDT-1, the levels of Cd and DDs in the co-contaminated soil decreased by 32.1-40.3% and 33.9-37.6%, respectively, in a pot experiment over 18 months compared to 3.25% and 3.76% decreases in soil Cd and DDs, respectively, in unplanted, untreated controls. A subsequent field study (18-month duration) in which the levels of Cd and DDs decreased by 31.1% and 53.6%, respectively, confirmed the beneficial results of this approach. This study demonstrates that the integrated bioremediation strategy is effective for the remediation of Cd-DDs co-contaminated soils.
Asunto(s)
Cadmio/metabolismo , DDT/metabolismo , Pseudomonas/metabolismo , Sedum/metabolismo , Contaminantes del Suelo/metabolismo , Biodegradación AmbientalRESUMEN
The selection and breeding of pollution-safe cultivars (PSCs) is a practicable and cost-effective approach to minimize the influx of heavy metal to the human food chain. In this study, both pot-culture and field experiments were conducted to identify and screen out cadmium pollution-safe cultivars (Cd-PSCs) from 50 pakchoi (Brassica rapa L. ssp. chinensis) cultivars for food safety. When treated with 1.0 or 2.5 mg/kg Cd, most of the pakchoi cultivars (>70%) showed greater or similar shoot biomass when compared with the control. This result indicates that pakchoi has a considerable tolerance to soil Cd stress. Cd concentrations in the shoot varied significantly (P<0.05) between cultivars: in two Cd treatments (1.0 and 2.5 mg/kg), the average values were 0.074 and 0.175 mg/kg fresh weight (FW), respectively. Cd concentrations in the shoots of 14 pakchoi cultivars were lower than 0.05 mg/kg FW. In pot-culture experiments, both enrichment factors (EFs) and translocation factors (TFs) of six pakchoi cultivars were lower than 1.0. The field studies further confirmed that the Hangzhouyoudonger, Aijiaoheiye 333, and Zaoshenghuajing cultivars are Cd-PSCs, and are therefore suitable for growth in low Cd-contaminated soils (≤1.2 mg/kg) without any risk to food safety.
Asunto(s)
Brassica rapa/metabolismo , Brassica rapa/toxicidad , Cadmio/metabolismo , Cadmio/toxicidad , Contaminación de Alimentos/prevención & control , Brassica rapa/crecimiento & desarrollo , China , Contaminación de Alimentos/análisis , Inocuidad de los Alimentos/métodos , Humanos , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/toxicidad , Especificidad de la EspecieRESUMEN
The influences of microorganism, soil moisture and cadmium (Cd) on degradation of carbendazim in paddy soil were investigated with laboratory microcosm experiments. The results showed that the half-life of carbendazim (5.0 mg x kg(-1) and 10.0 mg x kg(-1)) in sterilized soils was 12.6-13.8 times of those in non-sterilized soils. The half-life of carbendazim was decreased by 32.1%-37.1% when the soils were inoculated with carbendazim-degrading strains. When the soil moisture was increased from 40% to 60% or 80% of water holding capacity, the half life of carbendazim was decreased by 46.2% or 74.0%, respectively. Low level of Cd (5 mg x kg(-1)) enhanced the degradation of carbendazim in soils with half-life time decreased by 32.1%-52.4%, but high level of Cd (50 mg x kg(-1)) inhibited the degradation of carbendazim in soils with half-life time increased by 92.6%-103.0%. For the soils inoculated with carbendazim-degrading strains, the half life of carbendazim was decreased by 34.0% -34.4% with the addition of low level of Cd (5.0 mg x kg(-1)), while the half life time was increased by 74.4% -109.4% with the addition of high level of Cd (50 mg x kg(-1)). The results demonstrate that indigenous microorganisms is a critical factor that influences carbendazim degradation in soils, and that carbendazim-degrading strains, high soil moisture and low Cd level enhance the degradation of carbendazim.
Asunto(s)
Bencimidazoles/aislamiento & purificación , Carbamatos/aislamiento & purificación , Productos Agrícolas/crecimiento & desarrollo , Microbiología del Suelo , Contaminantes del Suelo/aislamiento & purificación , Bacterias/metabolismo , Bencimidazoles/metabolismo , Biodegradación Ambiental , Carbamatos/metabolismo , Suelo/química , Contaminantes del Suelo/metabolismoRESUMEN
A pot experiment was conducted to study the differences in cadmium (Cd) absorption and accumulation of sixty Brassica varieties on the soils added with different concentration of Cd. The results showed that when the soil Cd concentration was 0.6 mg x kg(-1) and 1.2 mg x kg(-1), the percentage of test Brassica varieties whose Cd content exceeded the National Edible Standard was 8.33% and 66.67%, respectively, suggesting that Brassica was easy to be Cd-polluted. The shoot Cd content of all test varieties was below the National Edible Standard, and the biomass of the varieties Changgengbaicai, Shanghaiqing, Aijisuzhouqing, Qingyou 4, Aijiaokuipianheiyebaicai, Zhouyeheiyoudonger, Gaohuaqinggengbaicai, Zaoshenghuajing, Jinguanqingjiangbai, Xiawangqinggengcai, Lifengqinggengbaicai, and Hangzhouyoudonger was not affected. These 12 Brassica varieties could be planted on the soils with light Cd pollution.
Asunto(s)
Brassica/metabolismo , Cadmio/metabolismo , Cadmio/toxicidad , Contaminantes del Suelo/metabolismo , Absorción , Biodegradación Ambiental , Brassica/clasificación , Brassica/crecimiento & desarrollo , China , Variación Genética , Contaminantes del Suelo/toxicidad , Especificidad de la EspecieRESUMEN
Sedum alfredii is a well known cadmium (Cd) hyperaccumulator native to China; however, the mechanism behind its hyperaccumulation of Cd is not fully understood. Through several hydroponic experiments, characteristics of Cd uptake and translocation were investigated in the hyperaccumulating ecotype (HE) of S. alfredii in comparison with its non-hyperaccumulating ecotype (NHE). The results showed that at Cd level of 10 microM measured Cd uptake in HE was 3-4 times higher than the implied Cd uptake calculated from transpiration rate. Furthermore, inhibition of transpiration rate in the HE has no essential effect on Cd accumulation in shoots of the plants. Low temperature treatment (4 degrees C) significantly inhibited Cd uptake and reduced upward translocation of Cd to shoots for 9 times in HE plants, whereas no such effect was observed in NHE. Cadmium concentration was 3-4-fold higher in xylem sap of HE, as compared with that in external uptake solution, whereas opposite results were obtained for NHE. Cadmium concentration in xylem sap of HE was significantly reduced by the addition of metabolic inhibitors, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (DNP), in the uptake solutions, whereas no such effect was noted in NHE. These results suggest that Cd uptake and translocation is an active process in plants of HE S. alfredii, symplastic pathway rather than apoplastic bypass contributes greatly to root uptake, xylem loading and translocation of Cd to the shoots of HE, in comparison with the NHE plants.
Asunto(s)
Cadmio/metabolismo , Sedum/metabolismo , Xilema/metabolismo , Transporte Biológico , Calcio/metabolismo , Exudados de Plantas/metabolismo , Raíces de Plantas/metabolismo , Brotes de la Planta/metabolismo , Transpiración de Plantas , Temperatura , Factores de TiempoRESUMEN
The phytotoxicity and antioxidative adaptations of lead (Pb) accumulating ecotype (AE) and non-accumulating ecotype (NAE) of Sedum alfredii Hance were investigated under different Pb treatments involving 0, 0.02 mmol/L Pb, 0.1 mmol/L Pb and 0.1 mmol/L Pb/0.1 mmol/L ethylenediaminetetraacetic acid (EDTA) for 6 days. With the increasing Pb level, the Pb concentration in the shoots of AE plants enhanced accordingly, and EDTA supply helped 51% of Pb translocation to shoots of AE compared with those treated with 0.1 mmol/L Pb alone. Moreover, the presence of EDTA alleviated Pb phytotoxicity through changes in plant biomass, root morphology and chlorophyll contents. Lead toxicity induced hydrogen peroxide (H2O2) accumulation and lipid peroxidation in both ecotypes of S. alfredii. The activities of superoxide dismutase (SOD), guaiacol peroxidase (G-POD), ascorbate peroxidase, and dehydroascorbate reductase elevated in both leaves and roots of AE as well as in leaves of NAE with the increasing Pb levels, but SOD and G-POD declined in roots of NAE. Enhancement in glutathione reductase activity was only detected in roots of NAE while a depression in catalase activity was recorded in the leaves of NAE. A significant enhancement in glutathione and ascorbic acid (AsA)levels occurred in both ecotypes exposed to Pb and Pb/EDTA treatment compared with the control, however, the differences between these two treatments were insignificant. The dehydroascorbate (DHA) contents in roots of both ecotypes were 1.41 to 11.22-fold higher than those in leaves, whereas the ratios of AsA to DHA (1.38 to 6.84) in leaves altering more to the reduced AsA form were much higher than those in roots. These results suggested that antioxidative enzymes and antioxidants play an important role in counteracting Pb stress in S. alfredii.
Asunto(s)
Antioxidantes/metabolismo , Plomo/farmacología , Sedum/crecimiento & desarrollo , Sedum/metabolismo , Ácido Ascórbico/metabolismo , Ácido Deshidroascórbico/metabolismo , Ácido Edético/farmacología , Glutatión/metabolismo , Peróxido de Hidrógeno/metabolismo , Plomo/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Fotosíntesis/efectos de los fármacos , Pigmentos Biológicos/metabolismo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/metabolismo , Sedum/efectos de los fármacos , Sedum/enzimologíaRESUMEN
Sedum alfredii (Crasulaceae) is the only known Cd-hyperaccumulating species that are not in the Brassica family; the mechanism of Cd hyperaccumulation in this plant is, however, little understood. Here, a combination of radioactive techniques, metabolic inhibitors, and fluorescence imaging was used to contrast Cd uptake and translocation between a hyperaccumulating ecotype (HE) and a non-hyperaccumulating ecotype (NHE) of S. alfredii. The K(m) of (109)Cd influx into roots was similar in both ecotypes, while the V(max) was 2-fold higher in the HE. Significant inhibition of Cd uptake by low temperature or metabolic inhibitors was observed in the HE, whereas the effect was less pronounced in the NHE. (109)Cd influx into roots was also significantly decreased by high Ca in both ecotypes. The rate of root-to-shoot translocation of (109)Cd in the HE was >10 times higher when compared with the NHE, and shoots of the HE accumulated dramatically higher (109)Cd concentrations those of the NHE. The addition of the metabolic inhibitor carbonyl cyanide m-chlorophenylhydrazone (CCCP) resulted in a significant reduction in Cd contents in the shoots of the HE, and in the roots of the NHE. Cd was distributed preferentially to the root cylinder of the HE but not the NHE, and there was a 3-5 times higher Cd concentration in xylem sap of the HE in contrast to the NHE. These results illustrate that a greatly enhanced rate of root-to-shoot translocation, possibly as a result of enhanced xylem loading, rather than differences in the rate of root uptake, was the pivotal process expressed in the Cd hyperaccumulator HE S. alfredii.
Asunto(s)
Cadmio/metabolismo , Raíces de Plantas/metabolismo , Brotes de la Planta/metabolismo , Sedum/metabolismo , Biodegradación Ambiental , Cationes Bivalentes/metabolismo , Frío , Cinética , Xilema/metabolismoRESUMEN
The study with pot experiment and simulation test showed that after planted hyperaccumulator Sedum alfredii Hance on mining soil, the water soluble Zn and NH4OAc extractable Zn in rhizosphere were decreased obviously, while the available Zn in non-rhizosphere had less change. The pH value of rhizosphere soil was decreased by 0. 3 units, whereas the organic matter and dissolved organic matter (DOM) contents were increased by 13.6% and 20.9%, respectively, compared with the soil without S. alfredii. The effects of DOM from S. alfredii rhizosphere on Zn absorption and desorption varied with the kinds of test soils. After the addition of rhizosphere DOM, the maximal absorption capacity (Xm) of mining soil, quaternary red clay soil, and fluavio-marine yellow loamy soil was reduced by 17.8%, 21.9% and 27.7%, respectively, whereas the addition of non-rhizosphere DOM had no effects on Zn absorption. The Zn desorption in the three soils, especially in fluavio-marine yellow loamy soil, was promoted by the addition of rhizosphere DOM. It was indicated that the DOM from S. alfredii rhizosphere could reduce the maximal absorption capacity and accelerate the desorption of adsorbed Zn, and thus, increase the Zn mobility and bioavailability.
Asunto(s)
Compuestos Orgánicos/metabolismo , Raíces de Plantas/metabolismo , Sedum/metabolismo , Suelo/análisis , Zinc/química , Adsorción/efectos de los fármacos , Compuestos Orgánicos/farmacología , Solubilidad , Zinc/análisisRESUMEN
Effects of cadmium (Cd) on microbial biomass, activity and community diversity were assessed in a representative variable charge soil (Typic Aquult) using an incubation study. Cadmium was added as Cd(NO3)(2) to reach a concentration range of 0-16 mg Cd/kg soil. Soil extractable Cd generally increased with Cd loading rate, but decreased with incubation time. Soil microbial biomass was enhanced at low Cd levels (0.5-1 mg/kg), but was inhibited consistently with increasing Cd rate. The ratio of microbial biomass C/N varied with Cd treatment levels, decreasing at low Cd rate (<0.7 mg/kg available Cd), but increasing progressively with Cd loading. Soil respiration was restrained at low Cd loading (<1 mg/kg), and enhanced at higher Cd levels. Soil microbial metabolic quotient (MMQ) was generally greater at high Cd loading (1-16 mg/kg). However, the MMQ is also affected by other factors. Cd contamination reduces species diversity of soil microbial communities and their ability to metabolize different C substrates. Soils with higher levels of Cd contamination showed decreases in indicator phospholipids fatty acids (PLFAs) for Gram-negative bacteria and actinomycetes, while the indicator PLFAs for Gram-positive bacteria and fungi increased with increasing levels of Cd contamination.
Asunto(s)
Cadmio/farmacología , Microbiología del Suelo , Biomasa , Carbono/metabolismo , Ácidos Grasos/metabolismo , Viabilidad Microbiana/efectos de los fármacos , Nitrógeno/metabolismo , Fosfolípidos/metabolismo , Factores de TiempoRESUMEN
Radiotracer techniques were employed to characterize (65)Zn adsorption and desorption in root-cell-wall of hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) species of Sedum alfredii Hance. The results indicated that at the end of a 30 min short time radioisotope loading period, comparable amounts of (65)Zn were accumulated in the roots of the two ecotypes Sedum alfredii, whereas 2.1-fold more (65)Zn remains in NHE root after 45-min desorption. At the end of 60 min uptake period, no difference of (65)Zn accumulation was observed in undesorbed root-cell-wall of Sedum alfredii. However, 3.0-fold more (65)Zn accumulated in desorbed root-cell-wall of NHE. Zn(2+) binding in root-cell-wall preparations of NHE was greater than that in HE under high Zn(2+) concentration. All these results suggested that root-cell-wall of the two ecotypes Sedum alfredii had the same ability to adsorb Zn(2+), whereas the desorption characteristics were different, and with most of (65)Zn binding on root of HE being available for loading into the xylem, as a result, more (65)Zn was translocated to the shoot.
Asunto(s)
Raíces de Plantas/metabolismo , Sedum/metabolismo , Zinc/farmacocinética , Adsorción , Biodegradación Ambiental , Células Cultivadas , Cinética , Tasa de Depuración Metabólica , Raíces de Plantas/citología , Sedum/citologíaRESUMEN
Sedum alfredii Hance has been reported to be a Zn-hyperaccumulator plant species. In this study, root morphological and physiological response of the hyperaccumulating ecotype of S. alfredii H. (HE) from the mined area and the non-hyperaccumulating ecotype of S. alfredii H. (NHE) from the agricultural area to supplied levels of Zn and Pb were investigated. The results showed that Zn concentrations in the leaves and the stems of the HE were 34 and 41 times higher, whereas lead concentrations were 1.9 and 2.4 times greater, respectively, than those of the NHE when grown at 1224 microM Zn and/or 200 microM Pb. At combined supply of 1224 microM Zn with 200 microM Pb, however, zinc concentrations in the stems and leaves of the, HE decreased, while lead concentrations in the stems increased significantly, as compared with those of single metal treatment. Lead uptake of the HE was enhanced by Zn addition. Root activity of the HE decreased by Pb treatment in the first two days, but recovered afterward and close to the control at day 10 of the treatment. However, root activity of the NHE decreased by each metal treatment, and was not recovered with the advance of treatment time. Root length, root surface area, and root volumes increased obviously due to Zn and/or Pb/Zn combined treatments for the HE, but significantly decreased due to Pb, Zn, or Pb/Zn combined treatment for the NHE. Zinc and Pb concentrations in both ecotypes of S. alfredii H. were positively correlated with root length, root surface area, and root volumes. Root exudates of the HE, especially treated with Zn, increased the extractability of Pb and Zn from the mined soil. At the Zn supply level of 1224microM, the extractability of root exudates on soil Pb was 3-12 times greater for the HE than for the NHE. These results imply that the tolerance and hyperaccumulation of the hyperaccumulating ecotype of S. alfredii H. to Zn and Pb appear to be closely related to its high adaptation of root growth, morphology, and physiology to Pb and Zn toxicity, and through its root excretion of some special substances that can activate Pb and Zn in the mined soil, thus increasing their mobilization and bioavailability.