[Characteristics of Water Extractable Organic Nitrogen from Erhai Lake Sediment and Its Differences with Other Sources].

The 47 samples from Erhai Lake surface sediments were collected in different seasons. The distribution and structure characteristics of sediment water extractable organic nitrogen(WEON) were investigated by using the combined techniques of UV-Vis absorption and three-dimensional excitation-emission matrix spectra(3DEEMs). The differences in DON of various sources(overlying water, pore water, inflow Rivers and wet deposition) were explored to analyze its effects on sediment. The results showed that:1the temporal distribution followed the pattern of summer > spring > autumn > winter, with the spatial WEON distribution of northern > southern > central. 2 The humic degree of Erhai sediment WEON was relatively high and mostly composed of fulvic acid, which mainly contained UV-like humic-like fluorescence peak A and high-excited tryptophan fluorescence peak B. This indicated that it was mainly affected by terrestrial input and microbial activity. 3 There were two fluorescent components(C1, C2) in the sediments and other sources of Erhai Lake. The component C1 was the endogenous visible ultraviolet peak formed by biodegradation, while the component C2 was the tryptophan peak. The bioavailability of wet deposition samples was comparatively high, greatly impacting Erhai Lake in heavy rainfall. The DON bioavailability in the inflow river was the lowest, which was prone to be accumulated in sediments. 4The DON component C1 and C2 in overlying water had significant negative correlation with Erhai sediment WEON contents(r=-0.79, P<0.01;r=-0.944, P<0.01). This suggested that the overlying water DON components could indirectly reflect the sediment WEON content of Erhai Lake, namely the higher the fluorescence components C1 and C2 in overlying water DON were, the lower the sediment WEON content was.

[Compositional Characteristics of Sediment Dissolved Organic Nitrogen in Typical Lakes and Its Relationship on Water Trophic Status].

UV-Vis absorbance, fluorescence, and Gas Chromatography Mass Spectrometry (GC-MS) were applied to the comparative study on sediment dissolved organic nitrogen (DON) in five typical lakes (Erhai lake, Dianchilake, Poyang lake, Wuhan Dong lake, and Taihu lake) in different lake regions with different nutrition status, revealing the relationship between structural and compositional characteristics of sediment DON and trophic level of lakes. The obtained results showed that: ①Structure of lake sediment DON in Yungui Plateau region is more stable, compared with those in Eastern Plain region, indicating its lower bioavailability. ②In Yungui Plateau region, the source and compositional characteristics of sediment DON weremore complex in Dianchi lake (a seriously polluted lake), and its sediment DON bioavailabilitywas relatively higher. While, with respect to the less polluted Erhai lake, the source of sediment DON is more simple with a higher stability in DON structure and composition, which is beneficial for maintaining its good water quality. ③In Eastern Plain region, nutrition status of Taihu lake was similer to Donghu lake. The structure and composition of sediment DON was complex. But the lower aromaticityand fewer Aromatic ring substituents abundance made their relatively weak nutrient retention ability, posing risk to water quality. With regard to Poyang Lake, the structure and composition of sediment DON was relatively simple, but nutrient retention ability was relatively strong, which played a positive role in maintaining good water quality. ④P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) values(the content ratio of humic-like substanceto protein-like substances)were in sequence of Dianchi Lake (33.14)>Erhai Lake(21.49)>Taihu Lake(9.06)>Donghu Lake(7.04)>Poyang Lake(4.83), while E(4)/E(6) values (the ratio of UV-Vis absorbance at 465 and 665 nm) were in sequence of Dianchi Lake (27.00)>Donghu Lake(6.65)>Poyang Lake(5.47)>Taihu(3.50)>Erhai Lake(2.31). In addition, P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) and E(4)/E(6) valueswere positively correlated with thecontents of the different nitrogen (N) forms in the sediments. The above information suggested that P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) and E(4)/E(6) values exhibited good discrimination degree among different trophic status lakes, and they were considered to indirectly indicate the nutrition levels of lakes to a certain extent.

[Investigation of an outbreak of acute diarrhea caused by Human calicivirus].

BACKGROUND: To survey a diarrhea outbreak in Guangan city and analyze the cause of the disease. METHODS: The population enrolled in the surveillance came from four different settings and was randomly sampled. Stool specimens collected from diarrhea patients were tested ordinarily for enteric bacteria and further examined for viral pathogens with PAGE, ELISA and RT-PCR. RESULTS: In total, 4,567 persons were surveyed, among them 942 had acute diarrhea (prevalence 20.63%). The incidence was higher in rural area (28.6%) than in urban area (19.6%) (chi-square =22.29, P less than 0.005) with a peak in May 10 through 25 four human caliciviruses were detected from stool specimens by ELISA and RT-PCR in specimens from 4 and 1 patients, respectively. CONCLUSION: Human calicivirus probably was the cause of this diarrhea outbreak in Guangan city.