Development of a New Sex Attractant via the Peripheral Coding of Pheromones in Mythimna loreyi.

Sex pheromones play an essential role when moths are searching for mates. Male olfactory receptor neurons (ORNs) are the primary determinant during peripheral pheromone recognition. Here, we identified the sex pheromones of a global agricultural pest, Mythimna loreyi, using gas chromatography coupled with mass spectrometry and electroantennographic detection. Nine pheromone components were identified, including (Z)-9-tetradecen-1-yl acetate (Z9-14:OAc), (Z)-7-dodecen-1-yl acetate (Z7-12:OAc), and (Z)-11-hexadecen-1-yl acetate (Z11-16:OAc), and the first two elicited electrophysiological activities in the male antennae. Trichoid sensilla were classified into four functional types on the basis of neuronal responses to pheromones by single sensillum recording. Five functional ORNs were involved in recognizing pheromones and pheromone analogues. Finally, a field bioassay revealed that a blend of Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc at a ratio of 100:8.8:19.7 was highly efficient for trapping males. Our results uncover the pheromone recognition mechanism in M. loreyi and provide a novel angle for developing efficient sex attractants of pests on the basis of screening the peripheral olfactory neurons.

[Spatial distribution and ecological significance of heavy metals in soils from Chatian mercury mining deposit, western Hunan province].

Ores, waste tailings and slag, together with three typical soil profiles (natural soil profiles far from mine entrance and near mine entrance, soil profile under slag) in Chatian mercury mining deposit (CMD), western Hunan province were sampled and their concentrations of mercury (Hg), arsenic (As), lead (Pb), cadmium (Cd), zinc (Zn) were determined by HG-ICP-AES and ICP-MS. Enrichment factor and correlation analysis were taken to investigate the origins, distribution and migration of Hg, as well as other heavy metals in the CMD. The results show that Hg is enriched in the bottom of the soil profile far from mine entrance but accumulated in the surface of soil profiles near mine entrance and under slag. The soil profiles near mine entrance and under slag are both contaminated by Hg, while the latter is contaminated more heavily. In the soil profile under slag, Hg concentration in the surface soil, Hg average concentration in the total profile, and the leaching depth of soil Hg are 640 microg x g(-1), (76.74 +/- 171.71) microg x g(-1), and more than 100 cm, respectively; while 6.5 microg x g(-1), (2.74 +/- 1.90) microg x g(-1), and 40 cm, respectively, are found in the soil profile near mine entrance. Soil in the mercury mine area is also polluted by Cd, As, Pb, Zn besides metallogenic element Hg, among which Cd pollution is relatively heavier than others. The mobility of the studied heavy metals in soil follows the order as Hg > Cd > As > Zn approximately equal to Pb. The leaching depth of the heavy metals is influenced by total concentration in the surface soil and soil physico-chemical parameters. The origins, distribution and migration of heavy metals in soil profile in the mining area are related to primary geological environment, and strongly influenced by human mining activities.