Effects of long-term no-tillage on the functional potential of microorganisms involved in the nitrogen, phosphorus and sulfur cycles of black soil.

Understanding the effects of different tillage practices on functional microbial abundance and composition in nitrogen (N), phosphorus (P) and sulfur (S) cycles are essential for the sustainable utilization of black soils. Based on an 8-year field experiment located in Changchun, Jilin Province, we analyzed the abundance and composition of N, P and S cycling microorganisms and their driving factors in different depths of black soil under no til-lage (NT) and conventional tillage (CT). Results showed that compared with CT, NT significantly increased soil water content (WC) and microbial biomass carbon (MBC) at soil depth of 0-20 cm. Compared with CT, NT significantly increased the abundances of functional and encoding genes related to N, P and S cycling, including the nosZ gene encoding N2O reductase, the ureC gene performing organic nitrogen ammoniation, the nifH gene encoding nitrogenase ferritin, the functional genes phnK and phoD driving organic phosphorus mineralization, the encoding pyrroloquinoline quinone synthase ppqC gene and the encoding exopolyphosphate esterase ppX gene, and the soxY and yedZ genes driving sulfur oxidation. The results of variation partitioning analysis and redundancy analysis showed that soil basic properties were the main factors affecting the microbial composition of N, P and S cycle functions (the total interpretation rate was 28.1%), and that MBC and WC were the most important drivers of the functional potential of soil microorganisms in N, P and S cycling. Overall, long-term no tillage could increase the abundance of functional genes of soil microorganisms by affecting soil environment. From the perspective of molecular biology, our results elucidated that no tillage could be used as an effective soil management measure to improve soil health and maintain green agricultural development.

[Research progress on soil aggregates and associated organic carbon in salinized soils]. / ç›æ¸åŒ–æ¡ä»¶ä¸‹åœŸå£¤å›¢èšä½“åŠå ¶æœ‰æœºç¢³ç ”ç©¶è¿›å±•.

Soil aggregate, as a basic component of soils, plays an important role in improving soil structure and enhancing soil organic carbon (SOC) sequestration. The special soil properties induced by salinization, such as high ion concentrations (mainly Na+), shortage of organic material and bad condition of microbe, inhibit the formation and stability of soil aggregate. Therefore, it is important and meaningful to explore the dynamics of aggregate in salinized soils. Coastal wetland and inland salinized marsh wetland are important salinized ecosystems. We systematically summarized the progress and achievements on soil aggregate in salinized agriculture and wetland ecosystems. Agricultural practices, such as organic and/or inorganic soil amendment application, tillage practice, vegetation type, straw return and saline water irrigation, advance the formation and stability of aggregate and aggregate-associated organic carbon in salinized soils. We discussed the problems and deficiency in the present studies of aggregate and aggregate-associated carbon in salinized soils as well as the research aspects and hot topics in the future. This review would be helpful for comprehensively understanding the advances and development directions on aggregate in salinized soils.

[Priming Effects of Soil Moisture on Soil Respiration Under Different Tillage Practices].

In the early stage of an incubation experiment, soil respiration has a sensitive response to different levels of soil moisture. To investigate the effects of soil moisture on soil respiration under different tillage practices, we designed an incubation trial using air-dried soil samples collected from tillage experiment station established on black soils in 2001. The tillage experiment consisted of no-tillage (NT), ridge tillage (RT), and conventional tillage (CT). According to field capacity (water-holding capacity, WHC), we set nine moisture levels including 30%, 60%, 90%, 120%, 150%, 180%, 210%, 240%, 270% WHC. During the 22-day short-term incubation, soil CO2 emission was measured. In the early stage of incubation, the priming effects occurred under all tillage practices. There were positive correlations between soil respiration and soil moisture. In addition to drought and flood conditions, soil CO2 fluxes followed the order of NT > RT > CT. We fitted the relationship between soil moisture and soil CO2 fluxes under different tillage practices. In the range of 30%-270% WHC, soil CO2 fluxes and soil moisture fitted a quadratic regression equation under NT, and linear regression equations under RT and CT. Under the conditions of 30%-210% WHC of both NT and RT, soil CO2 fluxes and soil moisture were well fitted by the logarithmic equation with fitting coefficient R² = 0.966 and 0.956, respectively.

[Effects of corn and soybean straws returning on CO2 efflux at initial stage in black soil].

In this study, the CO2 emission characteristics and its relationships with C and N concentration in soil amended with different types of residues were studied by thermostatic incubation method to investigate the decomposition characteristics of different types of residues after adding to the soil and the effect of C, N concentration in residues on carbon sequestration. The results showed that during 61 days incubation, the CO2 efflux rates in the soils added with the different residues changed over time and exhibited an initial decrease, followed by a stable low plateau, and then an increase to a high plateau and finally followed by a decrease. The characteristics of CO2 emissions varied with residues, with the differences mainly occurring in the starting and duration of the high plateau CO2 emission period. The cumulative CO2-C emission was significantly affected by residue type. The cumulative CO2-C emissions from soils amended with corn roots, bottom corn stalks, corn leaves, and soybean leaves (about 160 µmol · g(-1) of soil and residue) were significantly greater than those from soils amended with other residues for the initial 21 days. Except for soybean leaves, the cumulative soil CO2 emissions over the 61 day incubation period from soils amended with soybean residues were higher than that from soil amended with corn residues. There were significant linear relationships between the ratio of cumulative CO2-C emission to residue carbon concentration (CR), and both C/N and nitrogen concentration of residues in the initial 21 days incubation, but not for the entire 61 days incubation. Our study suggested that soil CO2 emission was closely dependent upon the type of residue. Soybean residues decomposed more easily than corn residues. However, the decay rate of soybean residues was slower than that of corn residues at the initial stage of incubation. Soil CO2 emission was significantly affected by the C/N ratios and nitrogen concentrations of crop residues only at the early phase of incubation.

[Effects of Different Residue Part Inputs of Corn Straws on CO2 Efflux and Microbial Biomass in Clay Loam and Sandy Loam Black Soils].

The decomposed rate of crop residues is a major determinant for carbon balance and nutrient cycling in agroecosystem. In this study, a constant temperature incubation study was conducted to evaluate CO2 emission and microbial biomass based on four different parts of corn straw (roots, lower stem, upper stem and leaves) and two soils with different textures (sandy loam and clay loam) from the black soil region. The relationships between soil CO2 emission, microbial biomass and the ratio of carbon (C) to nitrogen (N) and lignin of corn residues were analyzed by the linear regression. Results showed that the production of CO2 was increased with the addition of different parts of corn straw to soil, with the value of priming effect (PE) ranged from 215. 53 µmol . g-1 to 335. 17 µmol . g -1. Except for corn leaves, the cumulative CO2 production and PE of clay loam soil were significantly higher than those in sandy loam soil. The correlation of PE with lignin/N was obviously more significant than that with lignin concentration, nitrogen concentration and C/N of corn residue. The addition of corn straw to soil increased the contents of MBC and MBN and decreased MBC/MBN, which suggested that more nitrogen rather than carbon was conserved in microbial community. The augmenter of microbial biomass in sandy loam soil was greater than that in clay loam soil, but the total dissolved nitrogen was lower. Our results indicated that the differences in CO2 emission with the addition of residues to soils were primarily ascribe to the different lignin/N ratio in different corn parts; and the corn residues added into the sandy loam soil could enhance carbon sequestration, microbial biomass and nitrogen holding ability relative to clay loam soil.

[Impact of tillage practices on microbial biomass carbon in top layer of black soils].

A study was conducted on a long-term (13 years) tillage and rotation experiment on black soil in northeast China to determine the effects of tillage, time and soil depth on soil microbial biomass carbon (MBC). Tillage systems included no tillage (NT), ridge tillage (RT) and mould-board plough (MP). Soil sampling was done at 0-5, 5-10 and 10-20 cm depths in June, August and September, 2013, and April, 2014 in the corn phase of corn-soybean rotation plots. MBC content was measured by the chloroform fumigation extraction (CFE) method. The results showed that the MBC content varied with sampling time and soil depth. Soil MBC content was the lowest in April for all three tillage systems, and was highest in June for MP, and highest in August for NT and RT. At each sampling time, tillage system had a significant effect on soil MBC content only in the top 0-5 cm layer. The MBC content showed obvious stratification under NT and RT with a higher MBC content in the top 0-5 cm layer than under MP. The stratification ratios under NT and RT were greatest in September when they were respectively 67.8% and 95.5% greater than under MP. Our results showed that soil MBC contents were greatly affected by the time and soil depth, and were more apparently accumulated in the top layer under NT and RT.

[Research methods of carbon sequestration by soil aggregates: a review].

To increase soil organic carbon content is critical for maintaining soil fertility and agricultural sustainable development and for mitigating increased greenhouse gases and the effects of global climate change. Soil aggregates are the main components of soil, and have significant effects on soil physical and chemical properties. The physical protection of soil organic carbon by soil aggregates is the important mechanism of soil carbon sequestration. This paper reviewed the organic carbon sequestration by soil aggregates, and introduced the classic and current methods in studying the mechanisms of carbon sequestration by soil aggregates. The main problems and further research trends in this study field were also discussed.

[Effects of tillage mode on black soil's penetration resistance and bulk density].

Taking an eight-year field experiment site in Dehui County of Jilin Province, Northeast China as test object, this paper studied the effects of different tillage modes (no tillage and ploughing in autumn) on the penetration resistance and bulk density of black soil. No tillage increased the soil penetration resistance, especially at the soil depth of 2.5-17.5 cm. In the continuous cropping of maize and the rotation of maize-soybean, the maximum soil penetration resistance at planting zone under no tillage and ploughing in autumn was 2816 and 1931 kPa, and 2660 and 2051 kPa, respectively, which had no restriction on the crop growth. The curve of soil penetration resistance under ploughing in autumn changed with ridge shape, while that under no tillage changed less. Comparing with ploughing in autumn, no tillage increased the bulk density of 5-20 cm soil layer significantly. Under no tillage, the bulk density of 5-30 cm soil layer changed little, but under ploughing in autumn, soil bulk density increased gradually with increasing soil depth. There was no significant correlation between soil bulk density and soil penetration resistance.

[Prediction of soil organic carbon in different soil fractions of black soils in Northeast China using near-infrared reflectance spectroscopy].

The soil organic carbon (SOC) associated with different soil fractions varies in the composition and dynamics. The present work is aimed to evaluate the potential of near infrared spectroscopy (NIRS) to predict SOC content in different soil fractions of black soils. SOC contents of 136 black soil samples in China were analyzed and the NIR spectra were collected using a VECTOR/22 (Fourier transform infrared spectroscopy). Partial least squares (PLS) regression with cross validation was used to develop calibrations between reference data and NIRS spectra (n = 100) which were validated using an independent set of samples (n = 36). Predictions for water-sieved aggregate associated organic carbon were generally good with R2 (coefficient of determination) ranging from 0.69 to 0.82 and the RPD (residual prediction deviation) from 1.2 to 1.8. NIRS well predicted the SOC in < 53 microm mineral fraction (R2 = 0.97, RPD = 5.4), but the prediction for SOC in 250-2 000 microm or in 53-250 microm particulate matter fractions was poor. However, the prediction for the SOC in 53-2 000 microm fraction was good (R2 = 0.79, RPD = 2.2). In addition, NIRS very well predicted the SOC in fine particle fraction (< 20 microm) (R2 = 0.93, RPD = 3.8). Accordingly, NIRS showed a good potential to predict SOC in some soil fractions and could reduce tedious laboratory analysis.

[Assessment of humic and fulvic acids in black soils using near-infrared reflectance spectroscopy].

The organic carbon content and optical densities of humic acids in black soils of China were predicted and assessed using near infrared spectroscopy technique. The contents of humic acid (HA) and fulvic acid (FA) in 136 black soil samples in China were analyzed and the NIR spectra were collected using a VECTOR/22 (Fourier transform infrared spectroscopy). Partial least squares (PLS) regression with cross validation was used to develop prediction models with reference data and soil NIRS spectra, and the model was validated using an independent set of samples. NIRS well predicted (HAC+FAC), HAC and FAC contents, with R2 = 0.92, 0.92 and 0.86, RPD = 3.66, 3.82 and 2.69, and high correlation coefficients between predicted and measured values (r = 0.90, 0.85 and 0.82). Predictions for the E4 values of HA and FA were also good (R2 = 0.85, 0.85; RPD = 2.88, 2.65; r = 0.92, 0.80). Predictions for optical densities of HA and FA at 665 nm (E6) was acceptable. Generally, NIRS showed a good potential to predict C content and optical densities of humic acid and fulvic acid in blacks soils and may reveal information on SOC quality.

[Field sampling methods for earthworm: a review].

As a critical component of soil ecosystem, earthworm can improve soil structure and relates closely to soil nutrient cycling, playing an important role in promoting soil quality and productivity. However, there is lack of systematic study on the field sampling methods for earthworm, especially in China. This paper reviewed the operational processes of commonly used field sampling methods for earthworm, and discussed their corresponding merits, efficacy, and potential influence on research results. To achieve a complete and accurate characterization of earthworm community size and structure, the method of chemical repellent combined with hand-sorting could work well at the sites where physical disturbance was acceptable, while the AITC (allyl isothiocyanate) method would be a favorable option at the sites where soil destruction was not feasible.

[Near infrared spectroscopy in determining organic carbon and total nitrogen in black soil of Northeast China].

In this study, near infrared reflectance spectroscopy (NIRS) was used to determine the organic carbon (OC), total nitrogen (TN), and C/N ratio in black soil of Northeast China. Based on the 3699-12000 cm(-1) NIRS of 136 black soil samples collected in 2004-2005, and by using partial least square (PLS), the related quantitative models were established. Leave-one-out cross validation showed that the OC and TN were well predicted, with the values of coefficient of determination (R2) being 0.92 and 0.91, RPD (the ratio of standard deviation of validation set to root mean square error of cross validation) being 3.45 and 3.36, and correlation coefficient (r) being 0.94 and 0.93 respectively, suggesting that NIRS had the potential to predict the OC and TN in black soil of Northeastern China. However, the C/N ratio was poorly predicted, with R2 = 0.61, RPD = 1.61, and r = 0.74, indicating that NIRS could not give reasonable prediction for the C/N ratio in black soil.

[Characters of infiltration and preferential flow of black soil in Northeast China under different tillage patterns].

By using dye tracer and double-ring infiltrometer techniques, the characters of infiltration and preferential flow of black soil under no-tillage (NT) and fall moldboard plow (MP) were compared after six years continuous management. The results showed that the infiltration rate was higher under NT than under MP. When the infiltration reached steady, the infiltration rate and accumulative infiltration capacity under NT were 1.35 and 1.44 times as high as those under MP, respectively. The penetration depth of methylene blue reached a depth of 43 cm in NT soil, being 16 cm deeper than that in MP soil. Comparing with MP soil, NT soil had better development of pore structure and more biological pores, and presented better preferential flow character, which were of importance for water infiltration and soil and water conservation.

[Distribution of water-stable aggregates and aggregate-associated C in black soil in Northeast China].

The study on the distribution characters of water-stable aggregates and aggregate-associated C in 32 pairs of virgin and cultivated black soils in Northeast China showed that in virgin soils, the amount of water-stable macroaggregates (>0.25 mm) and their associated C in 0-30 cm layer were higher than those of microaggreagtes (< 0.25 mm). With the increase of soil depth, the amount of macroaggregates and their associated C showed a decreasing trend, while those of microaggregates increased significantly (P < 0.01). The distribution characters of aggregates in cultivated soils were in adverse to virgin soils. Compared with those in virgin soils, the amount of macroaggregates and their associated C in 0-30 cm layer in cultivated soils declined drastically, particularly for >1 mm aggregates. In virgin soils, the proportions of soil macroaggregates, especially for >1 mm aggregates, were positively correlated to soil organic carbon; but in cultivated soils, no obvious relationship was observed between them. The associated C of soil aggregates was positively correlated to soil total organic carbon in both virgin and cultivated soils (P <0.01). In cultivated soils, less C was associated with macroaggregates but 37% more C was associated with microaggreagtes, and the total soil organic carbon was decreased by 29.5%, in comparison with those in virgin soils, which suggested that soil macroaggregates played an important role in the dynamics changes of soil total organic carbon. It was indicated that soil water-stable macroaggregates were sensitive to soil management, and could be an indicator in evaluating the impacts of agronomic managements on soil fertility and soil quality.