RESUMEN
Diamide insecticides have gained popularity due to their high efficacy and low toxicity to nontarget organisms. However, diamide-associated resistance has emerged recently, causing a significant reduction in their potency, thereby hindering sustainable agricultural development. Here, we explored novel diamide insecticide analogs and, using a structure-based approach, rationally designed and synthesized 28 nitrophenyl substituted anthranilic diamides. Most of the compounds showed moderate to good activity against Mythimna separata, Plutella xylostella, and Spodoptera frugiperda. Among them, compounds Ia and Im showed extraordinarily high activity and their mode of action was verified on isolated neurons. Additionally, Im exhibited over 10-fold greater potency than chlorantraniliprole in a HEK293 cell line stably expressing S. frugiperda ryanodine receptors (SfRyRs) containing the resistance mutations, G4891E and I4734M. The binding modes of Im in the SfRyRs were predicted using in silico molecular docking analysis. Our novel nitrophenyl substituted anthranilic diamide derivatives provide valuable insights for the design of insecticidal RyR-targeting compounds to effectively control both wild type and diamide insecticide-resistant lepidopteran pests.
Asunto(s)
Insecticidas , Mariposas Nocturnas , Animales , Humanos , Diamida/farmacología , Simulación del Acoplamiento Molecular , Células HEK293 , Mariposas Nocturnas/genética , Spodoptera/metabolismo , Insecticidas/farmacología , Insecticidas/química , ortoaminobenzoatos/farmacología , ortoaminobenzoatos/química , Canal Liberador de Calcio Receptor de Rianodina/genética , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Resistencia a los Insecticidas/genéticaRESUMEN
Glucose oxidase (GOX) is a representative compound found in most insect saliva that can suppress plant-defensive responses. However, little is known about the origin and role of GOX in the crucifer-specialized pest Plutella xylostella. In this study, we showed obvious regurgitation from the larval gut of P. xylostella and identified abundant peptides highly similar to known GOX. Three PxGOX genes were verified with PxGOX2 preferentially expressed in the gut. The heterologously expressed PxGOX2 confirmed its function to be a GOX, and it was detected in plant wounds together with the gut regurgitant. Further experiments revealed that PxGOX2 functioned as an effector and may suppress defensive responses in plant through the production of H2O2, which modulates levels of antagonistic salicylic acid and jasmonic acid. However, excessive H2O2 in the host plant may be neutralized by peroxidase, thus forming defensive feedback. Our findings provided new insights into understanding the GOX-mediated insect-plant interactions.
RESUMEN
Ryanodine receptor (RyR) has been used as an insecticide target to control many destructive agricultural pests. The effectiveness of these insecticides has been limited by the spread of resistance mutations identified in pest RyRs, but the detailed molecular impacts of the individual mutations on the activity of different diamide compounds have not been fully explored. We created five HEK293 cell lines stably expressing wild type rabbit RyR1, wild type Spodoptera frugiperda RyR (Sf RyR), or Sf RyR carrying different resistance mutations, including G4891E, G4891E/I4734M, and Y4867F, respectively. R-CEPIA1er, a genetically encoded fluorescent protein, was also introduced in these cell lines to report the Ca2+ concentration in the endoplasmic reticulum. We systematically characterized the activities of six commercial diamide insecticides against different RyRs using the time-lapse fluorescence assay. Among them, cyantraniliprole (CYAN) displayed the highest activity against all three resistant Sf RyRs. The good performance of CYAN was confirmed by the toxicity assay using gene-edited Drosophila expressing the mutant RyRs, in which CYAN showed the lowest LD50 value for the double resistant mutant. In addition, we compared their acitivty between mammalian and insect RyRs and found that flubendiamide has the best insect-selectivity. The mechanism of the anti-resistance property and selectivity of the compounds was proposed based on the structural models generated by homology modeling and molecular docking. Our findings provide insights into the mechanism of insect resistance and guidance for developing effective RyR agonists that can selectively target resistant pests.
Asunto(s)
Insecticidas , Mariposas Nocturnas , Humanos , Animales , Conejos , Insecticidas/farmacología , Insecticidas/química , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Diamida/farmacología , Simulación del Acoplamiento Molecular , Células HEK293 , Resistencia a los Insecticidas/genética , Mariposas Nocturnas/genética , Mariposas Nocturnas/metabolismo , Mamíferos/metabolismoRESUMEN
Metabolic resistance is one of the most frequent mechanisms of insecticide resistance, characterized by an increased expression of several important enzymes and transporters, especially cytochrome P450s (CYPs). Due to the large number of P450s in pests, determining the precise relationship between these enzymes and the insecticide substrates is a challenge. Herein, we developed a luminescence-based screening system for efficient identification of insecticide substrates and insect P450 inhibitors. We recombinantly expressed Bemisia tabaci CYP6CM1vQ (Bt CYP6CM1vQ) in the fission yeast Schizosaccharomyces pombe and subsequently permeabilized the yeast cells to convert them into "enzyme bags". We exploited these enzyme bags to screen the activity of twelve luciferin substrates and identified Luciferin-FEE as the optimal competing probe that was further used to characterize the metabolism of eight candidate commercial insecticides. Among them, Bt CYP6CM1vQ exhibited notable activity against pymetrozine and imidacloprid. Their binding modes were predicted by homology modeling and molecular docking, revealing the mechanisms of the metabolism. We also tested the inhibitory effect of eight known P450 inhibitors using our system and identified letrozole and 1-benzylimidazole as showing significant activity against Bt CYP6CM1vQ, with IC50 values of 23.74 µM and 1.30 µM, respectively. Their potential to be developed as an insecticide synergist was further proven by an in vitro toxicity assay using imidacloprid-resistant Bemisia tabaci. Overall, our luciferin-based enzyme bag method is capable of providing a robust and efficient screening of insect P450 substrates and, more importantly, inhibitors to overcome the resistance.
Asunto(s)
Hemípteros , Insecticidas , Schizosaccharomyces , Animales , Insecticidas/farmacología , Insecticidas/metabolismo , Schizosaccharomyces/metabolismo , Simulación del Acoplamiento Molecular , Neonicotinoides/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Hemípteros/metabolismo , Resistencia a los InsecticidasRESUMEN
Snustorr snarlik (Snsl) is a type of extracellular protein essential for insect cuticle formation and insect survival, but is absent in mammals, making it a potential selective target for pest control. Here, we successfully expressed and purified the Snsl protein of Plutella xylostella in Escherichia coli. Two truncated forms of Snsl protein, Snsl 16-119 and Snsl 16-159, were expressed as a maltose-binding protein (MBP) fusion protein and purified to a purity above 90% after a five-step purification protocol. Snsl 16-119, forming stable monomer in solution, was crystallized, and the crystal was diffracted to a resolution of â¼10 Å. Snsl 16-159, forming an equilibrium between monomer and octamer in solution, was shown to form rod-shaped particles on negative staining electron-microscopy images. Our results lay a foundation for the determination of the structure of Snsl, which would improve our understanding of the molecular mechanism of cuticle formation and related pesticide resistance and provide a template for structure-based insecticide design.
Asunto(s)
Insecticidas , Mariposas Nocturnas , Animales , Mariposas Nocturnas/genética , Mariposas Nocturnas/metabolismo , Resistencia a los Insecticidas , Insecticidas/metabolismo , Larva , MamíferosRESUMEN
Ryanodine receptor (RyR) is a giant calcium release channel located on the membrane of the endoplasmic reticulum (ER). Here, we report the regulation of RyRs from two major agricultural pests, diamondback moth and fall armyworm, by insect calmodulin (CaM). The recombinantly expressed full-length insect RyR could be pulled down by insect CaM in the presence of Ca2+, but the efficiency is lower compared to rabbit RyR1 and insect RyR with the CaM-binding domain (CaMBD) replaced by rabbit RyR1 sequence. Interestingly, the enhanced binding of CaM in the mutant insect RyR resulted in an increased sensitivity to the diamide insecticide chlorantraniliprole (CHL), suggesting that this CaM-CaMBD interface could be targeted by potential synergists acting as molecular glue. The thermodynamics of the binding between insect CaM and CaMBD was characterized by isothermal titration calorimetry, and the key residues responsible for the insect-specific regulation were identified through mutagenesis studies.
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Calmodulina , Mariposas Nocturnas , Animales , Conejos , Calmodulina/genética , Calmodulina/química , Calmodulina/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Mariposas Nocturnas/genética , Mariposas Nocturnas/metabolismo , Señalización del Calcio , Unión Proteica , Calcio/metabolismoRESUMEN
Several mutations identified in phospholamban (PLN) have been linked to familial dilated cardiomyopathy (DCM) and heart failure, yet the underlying molecular mechanism remains controversial. PLN interacts with sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) and regulates calcium uptake, which is modulated by the protein kinase A (PKA)-dependent phosphorylation of PLN during the fight-or-flight response. Here, we present the crystal structures of the catalytic domain of mouse PKA in complex with wild-type and DCM-mutant PLNs. Our structures, combined with the results from other biophysical and biochemical assays, reveal a common disease mechanism: the mutations in PLN reduce its phosphorylation level by changing its conformation and weakening its interactions with PKA. In addition, we demonstrate that another more ubiquitous SERCA-regulatory peptide, called another-regulin (ALN), shares a similar mechanism mediated by PKA in regulating SERCA activity.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico , ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico , Animales , Proteínas de Unión al Calcio , Cardiomiopatía Dilatada , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Ratones , Retículo Sarcoplasmático/metabolismo , ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico/genética , ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico/metabolismoRESUMEN
Apolygus lucorum could cause severe economic damage to crops in China. The pest has been controlled by pyrethroids, and the target of pyrethroids is voltage-gated sodium channel (Nav). Double mutation (L1002F/D941G) was detected in a field-strain of A. lucorum . We found there was single mutation L1002F and double mutation L1002F/D941G, but no single mutation D941G in the field. The tail currents of L1002F and L1002F/D941G were reduced by two types pyrethroid. In contrast, D941G showed a similar activity as wild type channel. D941G and L1002F are both located in domain II but do not face the pyrethroid-binding pocket directly, suggesting that they might affect the insecticide-binding allosterically. L1002F/D941G has significantly different responses to pyrethroids compared to the wild type, but D941G alone has little effect compared to wild type. Our finding demonstrates that some mutation do not cause resistance by itself but can enhance the resistance combined with other mutations.
Asunto(s)
Heterópteros/genética , Resistencia a los Insecticidas , Insecticidas , Piretrinas , Animales , China , Resistencia a los Insecticidas/genética , Insecticidas/farmacología , Mutación , Piretrinas/farmacologíaRESUMEN
Idiopathic ventricular fibrillation (IVF) causes sudden death in young adult patients without structural or ischemic heart disease. Most IVF cases are sporadic and some patients present with short-coupled torsade de pointes, the genetics of which are poorly understood. A man who had a first syncope at the age of 35 presented with frequent short-coupled premature ventricular beats with bursts of polymorphic ventricular tachycardia and then died suddenly. By exome sequencing, we identified three rare variants: p.I784F in the SPRY1 of the ryanodine receptor 2 (RyR2), p.A96S in connexin 40 (Cx40), reported to affect electrical coupling and cardiac conduction, and a nonsense p.R244X in the cardiac-specific troponin I-interacting kinase (TNNI3K). We assessed intracellular Ca2+ handling in WT and mutant human RYR2 transfected HEK293 cells by fluorescent microscopy and an enhanced store overload-induced Ca2+ release in response to cytosolic Ca2+ was observed in RyR2-I784F cells. In addition, crystal structures and thermal melting temperatures revealed a conformational change in the I784F-SPRY1 domain compared to the WT-domain. The novel RyR2-I784F variant in SPRY1 domain causes a leaky channel under non-stress conditions. The presence of several variants affecting Ca2+ handling and cardiac conduction suggests a possible oligogenic origin for the ectopies originating from Purkinje fibres.
Asunto(s)
Proteínas de la Membrana/genética , Isquemia Miocárdica/genética , Fosfoproteínas/genética , Proteínas Serina-Treonina Quinasas/genética , Canal Liberador de Calcio Receptor de Rianodina/genética , Fibrilación Ventricular/genética , Adulto , Anciano , Anciano de 80 o más Años , Señalización del Calcio/genética , Conexinas/genética , Muerte Súbita/epidemiología , Femenino , Células HEK293 , Humanos , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/patología , Dominios Proteicos/genética , Torsades de Pointes/complicaciones , Torsades de Pointes/genética , Torsades de Pointes/patología , Fibrilación Ventricular/patología , Secuenciación del Exoma , Proteína alfa-5 de Unión ComunicanteRESUMEN
Cytosolic carboxypeptidases (CCPs) comprise a unique subfamily of M14 carboxypeptidases and are erasers of the reversible protein posttranslational modification- polyglutamylation. Potent inhibitors for CCPs may serve as leading compounds targeting imbalanced polyglutamylation. However, no efficient CCP inhibitor has yet been reported. Here, we showed that 2-phosphonomethylpentanedioic acid (2-PMPA), a potent inhibitor of the distant M28 family member glutamate carboxypeptidase II (GCPII), rather than the typical M14 inhibitor 2-benzylsuccinic acid, could efficiently inhibit CCP activities. 2-PMPA inhibited the recombinant Nna1 (a.k.a. CCP1) for hydrolyzing a synthetic peptide in a mixed manner, with Ki and Ki' being 0.11 µM and 0.24 µM respectively. It inhibited Nna1 for deglutamylating tubulin, the best-known polyglutamylated protein, with an IC50 of 0.21 mM. Homology modeling predicted that the R-form of 2-PMPA is more favorable to bind Nna1, unlike that GCPII prefers to S-form. This work for the first time identified a potent inhibitor for CCP family.
Asunto(s)
Glutamato Carboxipeptidasa II/antagonistas & inhibidores , Compuestos Organofosforados/farmacología , Inhibidores de Proteasas/farmacología , Carboxipeptidasas/antagonistas & inhibidores , Carboxipeptidasas/genética , Carboxipeptidasas/metabolismo , Citosol/enzimología , Evaluación Preclínica de Medicamentos/métodos , Proteínas de Unión al GTP/genética , Proteínas de Unión al GTP/metabolismo , Glutamato Carboxipeptidasa II/química , Glutamato Carboxipeptidasa II/metabolismo , Glutaratos/farmacología , Cinética , Simulación del Acoplamiento Molecular , Compuestos Organofosforados/química , Inhibidores de Proteasas/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , D-Ala-D-Ala Carboxipeptidasa de Tipo Serina/genética , D-Ala-D-Ala Carboxipeptidasa de Tipo Serina/metabolismo , Ácido Succínico/farmacologíaRESUMEN
The diamide insecticide class is one of the top-selling insecticides globally. They are used to control a wide range of pests by targeting their ryanodine receptors (RyRs). Here, we report the highest-resolution cryo-electron microscopy (cryo-EM) structure of RyR1 in the open state, in complex with the anthranilic diamide chlorantraniliprole (CHL). The 3.2-Å local resolution map facilitates unambiguous assignment of the CHL binding site. The molecule induces a conformational change by affecting the S4-S5 linker, triggering channel opening. The binding site is further corroborated by mutagenesis data, which reveal how diamide insecticides are selective to the Lepidoptera group of insects over honeybee or mammalian RyRs. Our data reveal that several pests have developed resistance via two mechanisms, steric hindrance and loss of contact. Our results provide a foundation for the development of highly selective pesticides aimed at overcoming resistance and therapeutic molecules to treat human myopathies.
Asunto(s)
Bloqueadores de los Canales de Calcio/metabolismo , Diamida/química , Insecticidas/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , ortoaminobenzoatos/metabolismo , Secuencia de Aminoácidos , Animales , Abejas , Sitios de Unión , Bloqueadores de los Canales de Calcio/química , Bloqueadores de los Canales de Calcio/farmacología , Microscopía por Crioelectrón , Desarrollo de Medicamentos , Resistencia a Medicamentos , Insecticidas/química , Insecticidas/farmacología , Lepidópteros , Modelos Moleculares , Mutagénesis/fisiología , Unión Proteica , Conformación Proteica , Transducción de Señal , Especificidad por Sustrato , ortoaminobenzoatos/química , ortoaminobenzoatos/farmacologíaRESUMEN
Ryanodine receptors (RyRs) are the molecular target of diamides, a new chemical class of insecticides. Diamide insecticides are used to control lepidopteran pests and were considered relatively safe for mammals and non-targeted beneficial insects, including honey bees. However, recent studies showed that exposure to diamides could cause long-lasting locomotor deficits of bees. Here we report the crystal structure of RyR N-terminal domain A (NTD-A) from the honeybee, Apis mellifera, at 2.5 Å resolution. It shows a similar overall fold as the RyR NTD-A from mammals and the diamondback moth (DBM), Plutella xylostella, and still several loops located at the inter-domain interfaces show insect-specific or bee-specific structural features. A potential insecticide-binding pocket formed by loop9 and loop13 is conserved in lepidopteran but different in both mammals and bees, making it a good candidate targeting site for the development of pest-selective insecticides. Furthermore, a conserved intra-domain disulfide bond was observed in both DBM and bee RyR NTD-A crystal structures, which explains their higher thermal stability compared to mammalian RyR NTD-A. This work provides a basis for the development of novel insecticides with better selectivity between pests and bees by targeting a distinct site on pest RyRs, which would be a promising strategy to overcome the current toxicity problem.
Asunto(s)
Abejas/metabolismo , Insecticidas/toxicidad , Canal Liberador de Calcio Receptor de Rianodina/química , Animales , Señalización del Calcio/efectos de los fármacos , Cristalografía/métodos , Diamida/toxicidad , Proteínas de Insectos/química , Proteínas de Insectos/efectos de los fármacos , Canal Liberador de Calcio Receptor de Rianodina/efectos de los fármacos , Canal Liberador de Calcio Receptor de Rianodina/aislamiento & purificaciónRESUMEN
Experimental 3D structures of calcium channels with phenylalkylamines (PAAs) provide basis for further analysis of atomic mechanisms of these important cardiovascular drugs. In the crystal structure of the engineered calcium channel CavAb with Br-verapamil and in the cryo-EM structure of the Cav1.1 channel with verapamil, the ligands bind in the inner pore. However, there are significant differences between these structures. In the crystal structure the ligand ammonium group is much closer to the ion in the selectivity-filter region Site 3, which is most proximal to the inner pore, than in the cryo-EM structure. Here we used Monte Carlo energy minimizations to dock PAAs in calcium channels. Our computations suggest that in the crystal structure Site 3 is occupied by a water molecule rather than by a calcium ion. Analysis of the published electron density map does not rule out this possibility. In the cryo-EM structures the ammonium group of verapamil is shifted from the calcium ion in Site 3 either along the pore axis, towards the cytoplasm or away from the axis. Our unbiased docking reproduced these binding modes. However, in the cryo-EM structures detergent and lipid molecules interact with verapamil. When we removed these molecules, the nitrile group of verapamil bound to the calcium ion in Site 3. Models of Cav1.2 with different PAAs suggest similar binding modes and direct contacts of the ligands electronegative atoms with the calcium ion in Site 3. Such interactions explain paradoxes in structure-activity relationships of PAAs.
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Bloqueadores de los Canales de Calcio/química , Canales de Calcio/metabolismo , Verapamilo/química , Secuencia de Aminoácidos , Sitios de Unión , Calcio/química , Cristalización , Ligandos , Simulación del Acoplamiento Molecular , Método de Montecarlo , Relación Estructura-ActividadRESUMEN
2(S)-dihydroxypropanesulfonate (DHPS) is a microbial degradation product of 6-deoxy-6-sulfo-d-glucopyranose (sulfoquinovose), a component of plant sulfolipid with an estimated annual production of 1010 tons. DHPS is also at millimolar levels in highly abundant marine phytoplankton. Its degradation and sulfur recycling by microbes, thus, play important roles in the biogeochemical sulfur cycle. However, DHPS degradative pathways in the anaerobic biosphere are not well understood. Here, we report the discovery and characterization of two O2-sensitive glycyl radical enzymes that use distinct mechanisms for DHPS degradation. DHPS-sulfolyase (HpsG) in sulfate- and sulfite-reducing bacteria catalyzes C-S cleavage to release sulfite for use as a terminal electron acceptor in respiration, producing H2S. DHPS-dehydratase (HpfG), in fermenting bacteria, catalyzes C-O cleavage to generate 3-sulfopropionaldehyde, subsequently reduced by the NADH-dependent sulfopropionaldehyde reductase (HpfD). Both enzymes are present in bacteria from diverse environments including human gut, suggesting the contribution of enzymatic radical chemistry to sulfur flux in various anaerobic niches.
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Alcanosulfonatos/metabolismo , Anaerobiosis , Bacterias/enzimología , Proteínas Bacterianas/metabolismo , Microbioma Gastrointestinal/fisiología , Biología Computacional , Pruebas de Enzimas , Sulfuro de Hidrógeno/metabolismo , Sulfuro de Hidrógeno/toxicidad , Metilglucósidos/metabolismo , Azufre/metabolismoRESUMEN
BACKGROUND: Pyrethroids are classified as type I and type II for distinct symptomology. Voltage-gated sodium channel is a primary target of pyrethroids. Mutations of the insect sodium channel have been identified to result in resistance to pyrethroids. Double mutation (L1002 F/M906 I) was detected in field-strain of Apolygus lucorum (Meyer-Dür). Although, it was illuminated the function of the same position mutation in other pests, it is necessary to demonstrate the role in A. lucorum . RESULTS: In this study, we examined the effects of mutations on channel gating and pyrethroid sensitivity in Xenopus oocytes. L1002 F, M906 I and L1002 F/M906 I all shifted the voltage dependence of activation in the depolarizing direction. L1002 F, M906 I and L1002 F/M906 I all reduced the amplitude of tail currents induced by type I (bifenthrin and permethrin) and type II (λ-cyhalothrin and deltamethrin). The double mutation, L1002 F/M906 I, reduced integral channel modification by 10-fold compared with the L1002 F and M906 I mutations alone, respectively. Computational analysis based on the model of dual pyrethroid receptors, the two resistance mutations, L1002 F and M906 I are facing two opposite sides of this newly identified pocket. Both mutations affect the optimal binding of the ligands by changing the shape of the pocket but in different ways. CONCLUSION: Our results illustrate the distinct effect of mutations on pyrethroids. It is predicted with computer modeling that these mutations allosterically affect pyrethroid binding. © 2020 Society of Chemical Industry.
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Heterópteros , Insecticidas , Piretrinas , Canales de Sodio Activados por Voltaje , Animales , Resistencia a los Insecticidas/genética , Insecticidas/farmacología , Mutación , Permetrina , Piretrinas/farmacología , Canales de Sodio Activados por Voltaje/genéticaRESUMEN
The diamondback moth, Plutella xylostella is a cosmopolitan pest that has evolved resistance to all classes of insecticide, and costs the world economy an estimated US $4-5 billion annually. We analyse patterns of variation among 532 P. xylostella genomes, representing a worldwide sample of 114 populations. We find evidence that suggests South America is the geographical area of origin of this species, challenging earlier hypotheses of an Old-World origin. Our analysis indicates that Plutella xylostella has experienced three major expansions across the world, mainly facilitated by European colonization and global trade. We identify genomic signatures of selection in genes related to metabolic and signaling pathways that could be evidence of environmental adaptation. This evolutionary history of P. xylostella provides insights into transoceanic movements that have enabled it to become a worldwide pest.
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Genoma de los Insectos/genética , Herbivoria/genética , Animales , Evolución Biológica , Entomología/métodos , Genética de Población/métodos , Filogenia , Transducción de Señal/genética , Transducción de Señal/fisiologíaRESUMEN
Ryanodine receptors (RyRs) are homotetrameric intracellular calcium (Ca2+) release channels responsible for excitation-contraction coupling of muscle cells. Diamide insecticides specifically act on RyRs of Lepidoptera and Coleoptera pests and are safe for nontargeted organisms, generating big worldwide sales. Despite their popularity, several devastating agricultural pests have been reported to be resistant to them because of mutations in a small transmembrane region of their RyRs, hinting a binding pocket nearby. A potential solution to overcome resistance is to develop new insecticides targeting different binding sites in pest RyRs. Based on a high-resolution crystal structure of diamondback moth (DBM) RyR N-terminal domain (NTD) determined by our group, we carried out extensive structure-based insecticide screening targeting the intersubunit interface. We identified eight lead compounds that selectively target the open conformation of DBM RyR, which are predicted to act as channel activators similar to diamide insecticides. Binding mode analysis shows selective binding to a hydrophobic pocket of DBM NTD-A but not to the pocket of its mammalian counterpart. We tested three available compounds on the HEK293 cell lines stably expressing DBM or mammalian RyR, one of which shows good potency and selectivity against DBM RyR. The insecticidal effect of the compound was also confirmed using fruit flies. The detailed binding mode, toxicity, absorption, distribution, metabolism, and excretion, and reactivity of the compound were predicted by bioinformatic methods. Together, our study lays a foundation for developing a new class of selective RyR-targeting insecticides to control both wild-type and resistant pests.
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Agonistas de los Canales de Calcio/farmacología , Proteínas de Insectos/antagonistas & inhibidores , Insecticidas/farmacología , Mariposas Nocturnas/efectos de los fármacos , Secuencia de Aminoácidos , Animales , Agonistas de los Canales de Calcio/química , Células HEK293 , Humanos , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Insecticidas/química , Mariposas Nocturnas/química , Mariposas Nocturnas/genética , Mariposas Nocturnas/metabolismo , Dominios Proteicos , Canal Liberador de Calcio Receptor de Rianodina/química , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Especificidad de la EspecieRESUMEN
Diamide insecticides targeting ryanodine receptors (RyRs) are a major class of pesticides used to control a wide range of agricultural pests, but their efficacies have been reduced dramatically by the recent emergence of resistance mutations. There is a pressing need to develop novel insecticides, targeting distinct and novel binding sites within insect RyRs to overcome the resistance crisis; however, the limited structural information on insect RyRs is a major roadblock to our understanding of their molecular mechanisms. Here, we report the crystal structure of the RyR SPRY2 domain from the diamondback moth (DBM), Plutella xylostella, a destructive agricultural pest worldwide that has developed resistance to all classes of insecticide at 2.06 Å resolution. The overall fold of DBM SPRY2 is similar to its mammalian homolog, but it shows distinct conformations in several loops. Docking it into the recently published cryo-electron microscope structure of the full-length RyR reveals that two insect-specific loops interact with the BSol domain from the neighboring subunit. The SPRY2-BSol interface will change the conformation upon channel gating, indicating that it might be a potential targeting site for insect-specific insecticides. Interestingly, several previously identified disease-causing mutations also lie in the same interface, implying that this interface is important for channel gating. Another insect-specific loop located in the SPRY2-SPRY3 interface might indirectly affect another gating interface between SPRY3 and Repeat34.
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Diamida/química , Proteínas de Insectos/química , Insecticidas/química , Mariposas Nocturnas/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/química , Animales , Diamida/farmacología , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Insecticidas/farmacología , Simulación del Acoplamiento Molecular , Mariposas Nocturnas/química , Mariposas Nocturnas/efectos de los fármacos , Mariposas Nocturnas/genética , Dominios Proteicos , Canal Liberador de Calcio Receptor de Rianodina/genética , Canal Liberador de Calcio Receptor de Rianodina/metabolismoRESUMEN
Evolutionary adaptations of herbivorous insects are often dictated by the necessity to withstand a corresponding evolutionary innovation in host plant defense. Glucosinolate sulfatase (GSS) enzyme activity is considered a central adaptation strategy in Plutella xylostella against glucosinolates (GS)-myrosinase defense system in the Brassicales. The high functional versatility of sulfatases suggests that they may perform other vital roles in the process of growth and development. Here, we used a CRISPR/Cas9 system to generate stable homozygous single/double mutant lines of gss1 or/and gss2 with no predicted off-target effects, to analyze the functions of the pair of duplicated genes in the development and host adaptation of P. xylostella. The bioassays showed that, when fed on their usual artificial diet, significant reduction in egg hatching rate and final larval survival rate of the single mutant line of gss2 compared with the original strain or mutant lines of gss1, revealing unexpected functions of GSS2 in embryonic and larval development. When larvae of homozygous mutant lines were transferred onto a new food, Arabidopsis thaliana, no induced effect at protein level of GSS1/2 or gene expression level of gss1/gss2 was detected. The absence of GSS1 or GSS2 reduced the survival rate of larvae and prolonged the duration of the larval stage, indicating that both GSS1 and GSS2 played an important role in adaptation to host plants. The versatile functions of duplicated GSSs in this study provide a foundation for further research to understand potential functions of other sulfatase members and support evidence of adaptation in herbivorous insects.
Asunto(s)
Adaptación Biológica , Genes de Insecto , Proteínas de Insectos/genética , Mariposas Nocturnas/genética , Sulfatasas/genética , Animales , Sistemas CRISPR-Cas , Femenino , Duplicación de Gen , Glucosinolatos/metabolismo , Proteínas de Insectos/metabolismo , Larva/enzimología , Larva/genética , Larva/crecimiento & desarrollo , Masculino , Mariposas Nocturnas/enzimología , Mariposas Nocturnas/crecimiento & desarrollo , Mutación , Sulfatasas/metabolismoRESUMEN
BACKGROUND: Diamide insecticides, including phthalic and anthranilic diamides, target insect ryanodine receptors (RyRs) and cause misregulation of calcium signaling in insect muscles and neurons. Several resistance mutations have been reported to reduce the efficacy of the diamides, but the exact binding sites and mechanism of resistance mutations are not clear. RESULTS: The recent breakthrough in structural studies of mammalian RyRs has deepened our understanding of these giant calcium-release channels, but structural information about insect RyRs is still scarce. The only reported high-resolution structure is from the N-terminal domain of diamondback moth (DBM) RyR determined by our group. Here, we generate several homology models of full-length DBM RyR representing different functional states and dock the diamide insecticides into the structural models using Schrodinger software. These models reveal the specific structural features, activation mechanism, structural difference between functional states, ligand-binding sites and insecticide-binding sites of DBM RyR. By comparing the structures of wild-type and insecticide-resistant mutants, we propose a model depicting how the mutations affect the insecticide binding. We also identify the key difference between mammalian and insect RyRs that may explain the species-specific binding properties of diamides. CONCLUSION: The binding sites for three activators Ca2+ , ATP and caffeine, and regulator ryanodine are conserved in insect and mammalian RyRs, but the binding site for diamide insecticides is species-specific. The phthalic and anthranilic diamides have distinct binding properties in DBM, which can be interfered by resistance mutations located in the transmembrane region. © 2019 Society of Chemical Industry.