RESUMEN
This study was aimed to investigate the distribution and implication of tap1 (transporter associated with antigen processing) and tap2 loci allelic and genotypic frequencies. The distribution of tap1 and tap2 loci allelic and genotypic frequencies in 339 random samples of healthy Chinese Hans was analyzed by TaqMan PCR. Several genetic information about power of discrimination, cumulative DP, polymorphism information content, expected heterozygosity and observed heterozygosity were calculated. The results indicated that 5 tap1 alleles (tap1*0101, 020101, 020102, 0301 and 0401) and 4 tap2 alleles (tap2*0101, 0102, 0103 and 0201) were detected in all samples. 8 tap1 genotypes were found which account for 53.3% of the theoretic genotype and 6 tap2 genotypes were found which account for 60% of the theoretic genotype. The genotyping results of tap1 and tap2 both conform to the Hardy-Weinberg expectations (p > 0.05). Tap1*0101 (79.79%) and tap2*0101 (82.74%) are the most common alleles in Chinese Hans. It is concluded that tap1*0101 and tap2*0101 are most common alleles in Chinese Hans, tap1 and tap2 loci carry some power of individual discrimination and polymorphism information content. These two locl can be used for the research in the fields of human genetics, linkage analysis of genetic disease genes, paternity test and individual identification and so on.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Alelos , Frecuencia de los Genes , Transportador de Casetes de Unión a ATP, Subfamilia B, Miembro 2 , Miembro 3 de la Subfamilia B de Transportadores de Casetes de Unión a ATP , Pueblo Asiatico/genética , Genotipo , Haplotipos , HumanosRESUMEN
According to the human platelet alloantigens (HPA) polymorphisms in five systems, the distributions of HPA-1 -3, 5, and 15 systems in 1 000 Chinese donors were carried out by using a polymerase chain reaction with sequence-specific primers (PCR-SSP) method. The genetic distance and phylogenetic tree between Chinese Hans and other populations were estimated by using DISPAN and PHYLIP software. As presented by the phylogenetic tree, Asian had a convergence with European first, and grouped together with African. Beninese which came from Africa was on the top of dendrogram. Indian was located between Asian and European. Brazilian was converged with other Europe populations. Oceanian Polynexiya had been shown specifically to cluster with Asia populations. These results proved the "out of Africa theory" from one side, and it also confirmed that early migration of Asian is from south to southeast, and east Asia., thus it is probable that Europeans are migrated from south to north, and west Europe. As genetic distance was estimated effectively by HPA systems, HPA systems could serve as the genetic marker in human migration and evolution research.
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Antígenos de Plaqueta Humana/genética , Filogenia , Antígenos CD/genética , Pueblo Asiatico , Población Negra , Proteínas Ligadas a GPI , Frecuencia de los Genes/genética , Humanos , Integrina beta3 , Proteínas de Neoplasias/genética , Reacción en Cadena de la Polimerasa , Grupos Raciales , Población BlancaRESUMEN
BACKGROUND: B(x) is a very rare ABO blood group phenotype and the molecular mechanism underlying it still remains largely unknown. This study reports two novel B(x) alleles in two Chinese individuals. STUDY DESIGN AND METHODS: Serologic investigations including serum transferase activity assay were performed with standard methods. DNA sequences of all seven exons and exon-intron boundaries of ABO gene were analyzed using genomic DNA by polymerase chain reaction and direct DNA sequencing or sequencing after gene cloning. RESULTS: B(x) phenotypes were diagnosed in these two individuals. DNA analysis revealed that the ABO gene of the two B(x) individuals was heterozygous of O01/B alleles. Two novel heterozygous mutations 905A>G and 541T>C were identified, respectively, which resulted in the amino acid changes D302G and W181R in the B glycosyltransferases. The mutations were not found in 120 randomly selected samples. CONCLUSION: Amino acid substitutions resulted from novel mutations 905A>G and 541T>C on ABO gene change highly conserved regions of the enzyme and may reduce the activity of the glycosyltransferases, leading to the B(x) phenotype.
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Sistema del Grupo Sanguíneo ABO/genética , Alelos , Galactosiltransferasas/genética , Mutación Missense , Mutación Puntual , Secuencia de Aminoácidos , Sustitución de Aminoácidos , China , Secuencia Conservada , Femenino , Galactosiltransferasas/química , Humanos , Datos de Secuencia Molecular , Fenotipo , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
HLA phenotypes of 26,266 Chinese individuals who were recruited as potential hematopoietic stem cell donors by the Shanghai Red Cross Marrow Donor Registry, part of the China Marrow Donor Program, were determined for HLA-A, -B, and -DRB1 alleles at low to intermediate resolution using DNA-based typing methods. The large sample size of the study allowed accurate calculation of the Chinese HLA haplotype frequencies. The observed alleles correspond to 19 HLA-A, 44 -B, and 13 -DR split antigens. The serologic equivalents of HLA-A36, -A80, -B78, and -DR18 alleles were not observed. A total of 2,241 distinct HLA-A, -B, -DRB1 haplotypes were identified. Three-locus haplotype frequency was estimated using the maximum likelihood method. The lowest haplotype frequency that can be reliably estimated at a 95% confidence level was 0.000057. Using this cutoff value, 1,220 haplotypes (54%) were statistically reliable and their cumulative haplotype frequency was 0.9730. The cumulative haplotype frequency of the remaining 1,021 haplotypes (46%) was 0.0270. A regression equation of p = 0.192 log N - 0.576 was derived to estimate the probability (p) of finding an HLA-A, -B, -DR split antigens-matched donor in a pool of N Chinese donors.
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Médula Ósea/inmunología , Dermatoglifia del ADN , Frecuencia de los Genes , Antígenos HLA-A/genética , Antígenos HLA-B/genética , Antígenos HLA-DR/genética , Pueblo Asiatico/genética , China , Haplotipos , Humanos , Sistema de RegistrosRESUMEN
OBJECTIVE: To study the polymorphism of human platelet alloantigens HPA-3 and HPA-9w in the Chinese Han population. METHODS: A total of 1000 unrelated Chinese Han blood donors from different provinces of China were genotyped for HPA-3 and HPA-9w using PCR-sequence specific primer assay. RESULTS: Gene frequencies of 1000 Chinese Hans for HPA-3a and HPA-3b were 0.5935 and 0.4065 respectively, and all of them were HPA-9a positive. The distributions of HPA-3, HPA-9w of Chinese Hans which detected by chi-square criterion fit Hardy-Weinberg equilibrium. There were significant differences of the HPA-3 alleles gene frequency between Guangdong province and other five investigated provinces which included Shanxi, Heilongjiang, Zhejiang, Yunnan and Jiangsu. In comparison to other ethnic groups, no significant differences were observed in the distributions of HPA-3 except the Vietnamese and Australian. CONCLUSION: The results show that the chance of HPA-3 incompatibility were 0.3661 in random transfusion, and also provide a basis for researching on alloimmune thrombocytopenia and HPA-matched transfusion.
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Antígenos de Plaqueta Humana/genética , Pueblo Asiatico/genética , Etnicidad/genética , Polimorfismo Genético , Alelos , Antígenos de Plaqueta Humana/inmunología , China/etnología , ADN/genética , Frecuencia de los Genes , Genotipo , Histocompatibilidad/genética , HumanosRESUMEN
A total of 1,000 Chinese blood donors were typed for human platelet antigens (HPA) using a sequence specific primers -polymerase chain reaction (PCR-SSP) based HPA genotyping method. An individual with a rare HPA-10w(a+b+) genotype was found. In order to confirm the typing results, a fragment of HPA-10 gene was amplified by PCR and then sequenced. Sequencing data showed that a single G to A substitution at nucleotide 263 occurred, resulting in amino acid change from Arg(CGA) to Gln(CAA) at position 62 of GPa protein. The substitution generated antigenic specificity HPA-10bw. The detection of an HPA-10bw allele in the Chinese population suggests that this rare allele should be considered in platelet alloimmunization, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion thrombocytopenic purpura (PTP) and post-transfusion refractoriness to platelets (PTR).
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Antígenos de Plaqueta Humana/genética , Polimorfismo de Nucleótido Simple , Alelos , Secuencia de Bases , China , Genotipo , Humanos , Integrina beta3/genética , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADNRESUMEN
Human platelet alloantigens (HPA) are specific antigens carried by platelet glycoproteins, which genes showing single nucleotide polymorphism. HPA can induce alloantibodies bringing about alloimmune response. They play important roles in post-transfusion refractoriness to platelets, post-transfusion thrombocytopenic purpura, fetomaternal alloimmune thrombocytopenia, and graft-versus-host disease. Because of their side effects in clinical blood-transfusion, there were a great deal of studies on HPA during last few decades. This review focuses on the nomenclature of HPA, the polymorphisms of platelet glycoproteins, HPA typing of the serological and molecular technology, as well as the mechanism of alloimmunization to HPA and correlated diseases.
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Antígenos de Plaqueta Humana/inmunología , Glicoproteínas de Membrana Plaquetaria/genética , Polimorfismo de Nucleótido Simple , Antígenos de Plaqueta Humana/clasificación , Humanos , Isoanticuerpos/inmunología , Reacción a la TransfusiónRESUMEN
OBJECTIVE: This is a study on some ABO subgroup samples which show discordant results of serological and molecular blood typing, the aim is to clarify their true ABO type by means of nucleotide analysis on exons 6 and 7 of their ABO gene. METHODS: Absorb-elution test and family investigation were conducted to study 7 samples which were involved in ABO grouping discrepancies. Duplex polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method was used to identify their ABO genotypes. PCR products of exons 6 and 7 were cloned and sequenced. RESULTS: All the 7 ABO subgroup samples with the discordant results of serological and molecular blood typing were found to have the normal O gene. Four out of them were typed as ABsub by serology, they were all of the A*102/O genotype. Sequencing analysis found all their A gene having the nt467 (C-->T) and nt803 (G-->C) mutation by comparison with the A*101 allele, i.e. their real type should be CisAB/O. Three out of 7 were typed as AsubB by serology and as BO by genotype; and point mutation was detected in all of their B gene. One of them had the nt700 (C-->G) mutation, the other 2 unrelated individuals had the novel nt640 (A-->G) mutation in their B alleles. CONCLUSION: Through nucleotide analysis, 7 samples have been typed as AB subgroup in serology with the normal O gene, their real ABO type being CisAB in 4 cases and B(A) in 3 cases. At the same time, a kind of novel B (A)640 allele has been uncovered in this study.
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Sistema del Grupo Sanguíneo ABO/genética , Mutación , Pueblo Asiatico/genética , Tipificación y Pruebas Cruzadas Sanguíneas , China , Femenino , Genotipo , Humanos , Masculino , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
The miscibility of CPP with alkyd resin in the specified mixing ratios has been studied by FTIR spectra. The results obtained indicate that the bands of hydroxyl groups noticeably shifted in the FTIR spectra of the blends in contrast with the infrared spectrum of alkyd resin, and the blends were miscible when the weight fraction of alkyd resin in the blends of CPP and alkyd resin was less than 0.5, and immiscible when greater than 0.5, which were proved by the glossiness of their films.
RESUMEN
A new desulfurizing absorbent for flue gas, i.e., an organic physical solvent of DMSO(dimethyl sulfoxide) mixed with a relatively small amount of chemical solvent (Mn2+) was studied. Compared with pure physical solvent of DMSO, the purification efficiency of the new absorbent was improved. And its absorption and reaction mechanism are discussed.