Comprehensive profiles of sulfur cycling microbial communities along a mangrove sediment depth.

The sulfur (S) cycle is an important biogeochemical cycle with profound implications for both cellular- and ecosystem-level processes by diverse microorganisms. Mangrove sediments are a hotspot of biogeochemical cycling, especially for the S cycle with high concentrations of S compounds. Previous studies have mainly focused on some specific inorganic S cycling processes without paying specific attention to the overall S-cycling communities and processes as well as organic S metabolism. In this study, we comprehensively analyzed the distribution, ecological network and assembly mechanisms of S cycling microbial communities and their changes with sediment depths using metagenome sequencing data. The results showed that the abundance of gene families involved in sulfur oxidation, assimilatory sulfate reduction, and dimethylsulfoniopropionate (DMSP) cleavage and demethylation decreased with sediment depths, while those involved in S reduction and dimethyl sulfide (DMS) transformation showed an opposite trend. Specifically, glpE, responsible for converting S2O32- to SO32-, showed the highest abundance in the surface sediment and decreased with sediment depths; in contrast, high abundances of dmsA, responsible for converting dimethyl sulfoxide (DMSO) to DMS, were identified and increased with sediment depths. We identified Pseudomonas and Streptomyces as the main S-cycling microorganisms, while Thermococcus could play an import role in microbial network connections in the S-cycling microbial community. Our statistical analysis showed that both taxonomical and functional compositions were generally shaped by stochastic processes, while the functional composition of organic S metabolism showed a transition from stochastic to deterministic processes. This study provides a novel perspective of diversity distribution of S-cycling functions and taxa as well as their potential assembly mechanisms, which has important implications for maintaining mangrove ecosystem functions.

Changes in planta K nutrient content altered the interaction pattern between Nicotiana benthamiana and Alternaria longipes.

Potassium (K) fertilisation has frequently been shown to enhance plant resistance against pathogens, though the mechanisms remain elusive. This study investigates the interaction dynamics between Nicotiana benthamiana and the pathogen Alternaria longipes under different planta K levels. On the host side, adding K activated the expressions of three NLR (nucleotide-binding domain and leucine-rich repeat-containing proteins) resistance genes, including NbRPM1, NbR1B23 and NbNBS12. Silencing these NLRs attenuated resistance in high-K (HK, 40.8 g/kg) plant, whereas their overexpression strengthened resistance in low-K (LK, 23.9 g/kg) plant. Typically, these NLRs mainly strengthened plant resistance via promoting the expression of pathogenesis-related genes (PRs), ROS burst and synthesis of antifungal metabolites in HK plant. On the pathogen side, the expression of effectors HKCSP1, HKCSP2 and LKCSP were shown to be related to planta K content. A. longipes mainly expressed effectors HKCSP1 and HKCSP2 in HK plant to interfere host resistance. HKCSP1 physically interacted with NbRPM1 to promote the degradation of NbRPM1, then attenuated related resistance in HK N. benthamiana. Meanwhile, HKCSP2 directly interacted with NbPR5 to suppress resistance in HK plant. In LK plant, A. longipes mainly deployed LKCSP that interacted with NbR1B23 to interfere reduce resistance in N. benthamiana. Overall, our research insights that both pathogen and host mobilise distinct strategies to outcompete each other during interactions in different K nutrient environments.

Natural-inspired hierarchic double-Janus solar evaporator for stable clean water production from high-salinity emulsions.

Interfacial solar evaporation shows great potential in clean water production, emulsions separation, and high-salinity brine treatment. However, it remains challenging for the evaporators to maintain a high evaporation rate in the high-salinity emulsions due to the co-pollution of salt and oil. Herein, we first proposed a hierarchic double-Janus solar evaporator (HDJE) with a hydrophobic salt-rejecting top layer and oil-rejecting bottom layer. Compared to the traditional one, HDJE could treat industrial high-salinity oil-in-water emulsions stably for over 70 h, with a stable average evaporation rate of 1.73 kg m-2 h-1 and a high purification efficiency of up to 99.8 % for oil and ions. It was also verified that HDJE could be used for high-efficiency purification of oily concentrated seawater outdoor. An average water production rate of 3.59 kg m-2 d-1 and a TOC removal ratio of over 98 % was obtained. In conclusion, this study provides a novel way to effectively dispose of high-salinity oily wastewater.

Structural Design and Energy and Environmental Applications of Hydrogen-Bonded Organic Frameworks: A Systematic Review.

Hydrogen-bonded organic frameworks (HOFs) are emerging porous materials that show high structural flexibility, mild synthetic conditions, good solution processability, easy healing and regeneration, and good recyclability. Although these properties give them many potential multifunctional applications, their frameworks are unstable due to the presence of only weak and reversible hydrogen bonds. In this work, the development history and synthesis methods of HOFs are reviewed, and categorize their structural design concepts and strategies to improve their stability. More importantly, due to the significant potential of the latest HOF-related research for addressing energy and environmental issues, this work discusses the latest advances in the methods of energy storage and conversion, energy substance generation and isolation, environmental detection and isolation, degradation and transformation, and biological applications. Furthermore, a discussion of the coupling orientation of HOF in the cross-cutting fields of energy and environment is presented for the first time. Finally, current challenges, opportunities, and strategies for the development of HOFs to advance their energy and environmental applications are discussed.

Enhanced metronidazole removal in seawater using a single-chamber bioelectrochemical system.

The aim of this study was to investigate the removal of metronidazole (MNZ) from seawater using a bioelectrochemical system (BES). Single-chamber BES (i.e., S-BES) and dual-chamber BES (i.e., D-BES) were constructed with carbon brush as the anode and cathode. With the inoculum of sea mud and 2 g/L of glucose as the substrate in seawater, S-BES and D-BES were acclimated to test the MNZ removal. Results showed that S-BES could remove almost 100 % of 200 mg/L MNZ within 120 h and remain stable within 10 cycles of operation (∼50 d) under the applied voltage of 0.8 V. The MNZ removal reached ∼100 % and 60.2 % in the cathodic and anodic chambers of D-BES fed by 100 mg/L MNZ under 0.8 V, respectively. The MNZ concentration of 200 mg/L significantly inhibited the sulfur metabolism, decreased the ratio of live to dead cells in the electrode biofilms, and thus reduced the SO42- removal in the S-BES. The MNZ degradation and S2- oxidation was mainly attributed to the cathodic and anodic biofilms of S-BES, respectively. Three degradation pathways of MNZ were proposed based on the identified intermediates and results of density functional theory calculations. The synergies among different genus species in the bacterial communities of biofilms, and between anodic and cathodic reactions could be responsible for the high performance of S-BES. Results from this study should be not only useful for the MNZ removal but also for effective MNZ inhibition of sulfate-reducing bacteria induced microbiologically influenced corrosion in seawater.

Major quality regulation network of flavonoid synthesis governing the bioactivity of black wolfberry.

Black wolfberry (Lycium ruthenicum Murr.) contains various bioactive metabolites represented by flavonoids, which are quite different among production regions. However, the underlying regulation mechanism of flavonoid biosynthesis governing the bioactivity of black wolfberry remains unclear. Presently, we compared the bioactivity of black wolfberry from five production regions. Multi-omics were performed to construct the regulation network associated with the fruit bioactivity. The detailed regulation mechanisms were identified using genetic and molecular methods. Typically, Qinghai (QH) fruit exhibited higher antioxidant and anti-inflammatory activities. The higher medicinal activity of QH fruit was closely associated with the accumulation of eight flavonoids, especially Kaempferol-3-O-rutinoside (K3R) and Quercetin-3-O-rutinoside (rutin). Flavonoid biosynthesis was found to be more active in QH fruit, and the upregulation of LrFLS, LrCHS, LrF3H and LrCYP75B1 caused the accumulation of K3R and rutin, leading to high medicinal bioactivities of black wolfberry. Importantly, transcription factor LrMYB94 was found to regulate LrFLS, LrCHS and LrF3H, while LrWRKY32 directly triggered LrCYP75B1 expression. Moreover, LrMYB94 interacted with LrWRKY32 to promote LrWRKY32-regulated LrCYP75B1 expression and rutin synthesis in black wolfberry. Transgenic black wolfberry overexpressing LrMYB94/LrWRKY32 contained higher levels of K3R and rutin, and exhibited high medicinal bioactivities. Importantly, the LrMYB94/LrWRKY32-regulated flavonoid biosynthesis was light-responsive, showing the importance of light intensity for the medicinal quality of black wolfberry. Overall, our results elucidated the regulation mechanisms of K3R and rutin synthesis, providing the basis for the genetic breeding of high-quality black wolfberry.

Autotrophic degradation of sulfamethoxazole using sulfate-reducing biocathode in microbial photo-electrolysis system.

Sulfamethoxazole is a representative of sulfonamide antibiotic pollutants. This study aims to investigate the degradation pathways of sulfamethoxazole and the response of microbial communities using the autotrophic biocathode in microbial photo-electrolysis systems (MPESs). Sulfamethoxazole with an initial concentration of 2 mg L-1 was degraded into small molecule propanol within 6 h with the biocathode. Elemental sulfur (S0) was detected in the cathode chamber, accounting for 57 % of the removed sulfate. The conversion from sulfate to S0 indicated that autotrophic microorganisms might adopt a novel pathway for sulfamethoxazole removal in the MPES. In the abiotic cathode, sulfamethoxazole degradation rate was 0.09 mg L-1 h-1 with the electrochemistry process. However, sulfamethoxazole was converted to products that still contain benzene rings, including p-aminothiophenol, 3-amino-5-methylisoxazole, and sulfonamide. The microbial community analysis indicated that the synergistic interaction of Desulfovibrio and Acetobacterium promoted the autotrophic degradation of sulfamethoxazole. The results suggested that autotrophic microorganisms may play an important role in the environmental transformation of sulfamethoxazole.

A fungal CFEM-containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity.

Colletotrichum fructicola causes a broad range of plant diseases worldwide and secretes many candidate proteinous effectors during infection, but it remains largely unknown regarding their effects in conquering plant immunity. Here, we characterized a novel effector CfEC12 that is required for the virulence of C. fructicola. CfEC12 contains a CFEM domain and is highly expressed during the early stage of host infection. Overexpression of CfEC12 suppressed BAX-triggered cell death, callose deposition and ROS burst in Nicotiana benthamiana. CfEC12 interacted with apple MdNIMIN2, a NIM1-interacting (NIMIN) protein that putatively modulates NPR1 activity in response to SA signal. Transient expression and transgenic analyses showed that MdNIMIN2 was required for apple resistance to C. fructicola infection and rescued the defence reduction in NbNIMIN2-silenced N. benthamiana, supporting a positive role in plant immunity. CfEC12 and MdNPR1 interacted with a common region of MdNIMIN2, indicating that CfEC12 suppresses the interaction between MdNIMIN2 and MdNPR1 by competitive target binding. In sum, we identified a fungal effector that targets the plant salicylic acid defence pathway to promote fungal infection.

Labile carbon-induced soil organic matter turnover in a subtropical forest under different redox conditions.

Labile organic carbon (LOC) input strongly affects soil organic matter (SOM) dynamics, including gains and losses. However, it is unclear how redox fluctuations regulate these processes of SOM decomposition and formation induced by LOC input. The objective of this study was to explore the impacts of LOC input on SOM turnover under different redox conditions. Soil samples were collected in a subtropical forest. A single pulse of 13C-labeled glucose (i.e., LOC) was applied to the soil. Soil samples were incubated for 40 days under three redox treatments, including aerobic, anoxic, and 10-day aerobic followed by 10-day anoxic conditions. Results showed that LOC input affected soil priming and 13C-SOM accumulation differently under distinct redox conditions by altering the activities of various microorganisms. 13C-PLFAs (phospholipid fatty acids) were analyzed to determine the role of microbial groups in SOM turnover. Increased activities of fungi and gram-positive bacteria (i.e., the K-strategists) by LOC input could ingest metabolites or residues of the r-strategists (e.g., gram-negative bacteria) to result in positive priming. Fungi could use gram-negative bacteria to stimulate priming intensity via microbial turnover in aerobic conditions first. Reduced activities of K-strategists as a result of the aerobic to anoxic transition decreased priming intensity. The difference in LOC retention in SOM under different redox conditions was mainly attributable to 13C-particulate organic carbon (13C-POC) accumulation. Under aerobic conditions, fungi and gram-positive bacteria used derivatives from gram-negative bacteria to reduce newly formed POC. However, anoxic conditions were not conducive to the uptake of gram-negative bacteria by fungi and gram-positive bacteria, favoring SOM retention. This work indicated that redox-regulated microbial activities can control SOM decomposition and formation induced by LOC input. It is extremely valuable for understanding the contribution of soil affected by redox fluctuations to the carbon cycle.

Efficient hydrogen production in single-chamber microbial electrolysis cell with a fermentable substrate under hyperalkaline conditions.

Hydrogen production from food waste is of great significance for energy conversion and pollution control. The aim of this study was to investigate the glucose fermentation from food waste and hydrogen (H2) production in the single-chamber microbial electrolysis cell (MEC) under hyperalkaline conditions. Single-chamber MECs were tested with 1 g/L glucose as substrate under different pH values (i.e., 7.0, 9.5, and 11.2) and applied voltages (i.e., 0.8, 1.2, and 1.6 V). With pH increase from 7.0 to 11.2, H2 production with methanogenesis inhibition was significantly improved in the MEC. At pH of 11.2, the maximum current density reached 180 ± 9 A/m3 with the H2 purity of 93.3 ± 1.2% and average H2 yield of 7.72 ± 0.23 mol H2/ mol glucose under 1.6 V. Acetate from glucose fermentation was the largest electron sink within 12 h. Methanobacterium alcaliphilum dominated the archaeal communities with the relative abundance of > 99.0% in the cathodic biofilms. The microbial communities and mcr A gene copy numbers analyses showed that high pH enhanced the acetate production from glucose fermentation, inhibited syntrophic acetate-oxidizing with hydrogenotrophic methanogenesis in the anodic biofilms, and inhibited hydrogenotrophic methanogenesis in the cathodic biofilms. Our results of hyperalkaline conditions provide a feasible way to harvest H2 efficiently from fermentable substrates in the single-chamber MEC.

Biohybrid magnetic microrobots: An intriguing and promising platform in biomedicine.

Biohybrid magnetic microrobots (BMMs) have emerged as an exciting class of microrobots and have been considered as a promising platform in biomedicine. Many microorganisms and body's own cells show intriguing properties, such as morphological characteristics, biosafety, and taxis abilities (e.g., chemotaxis, aerotaxis), which have made them attractive for the fabrication of microrobots. For remote controllability and sustainable actuation, magnetic components are usually incorporated onto these biological entities, and other functionalized non-biological components (e.g., therapeutic agents) are also included for specific applications. This review highlights the latest developments in BMMs with a focus on their biomedical applications. It starts by introducing the fundamental understanding of the propulsion system at the microscale in a magnetically driven manner, followed by a summary of diverse BMMs based on different microorganisms and body's own cells along with their relevant applications. Finally, the review discusses how BMMs contribute to the advancements of microrobots, the current challenges of using BMMs in practical clinical settings, and the future perspectives of this exciting field. STATEMENT OF SIGNIFICANCE: Biohybrid magnetic microrobots (BMMs), composed of biological entities and functional parts, hold great potential and serve as a novel and promising platform for biomedical applications such as targeted drug delivery. This review comprehensively summarizes the recent advancements in BMMs for biomedical applications, mainly focused on the representative propulsion modalities in a magnetically propelled manner and diverse designs of BMMs based on different biological entities, including microorganisms and body's own cells. We hope this review can provide ideas for the future design, development, and innovation of micro/nanorobots in the field of biomedicine.

Effect of polystyrene microplastics on tetracycline photoconversion under simulated sunlight: Vital role of aged polystyrene.

Photoconversion of tetracycline (TC) has been widely reported. However, the effect of microplastics (MPs) on TC conversion kinetics and mechanism has rarely been discussed. In this study, we investigated the effect of (aged) MPs on TC degradation under simulated sunlight and elucidated the underlying mechanism. Our findings demonstrated that the physical and chemical properties of polystyrene (PS), such as particle size, surface groups, and morphology, were significantly altered after aging. Moreover, photoconversion efficiency of TC was suppressed with the spiking of aged PS, while virgin PS showed an opposite tendency. The photodegradation reaction for photosensitization of PS involved 1O2 and HO·. The light-screening effect of aged PS occupied predominance, weakening the direct UV-light absorption of TC and resulting in lower TC degradation efficiency. Additionally, triplet-excited state PS was generated after photon acceptance by aged PS, which could transfer energy to O2, leading to the production of 1O2. The toxicity test manifested that the direct impact of TC products on fathead minnow was ignorable, but long-term negative effects on growth deserved observation. This study enhances our understanding of the environmental fate of PS and TC under sunlight, and provides crucial reference information for better evaluating the potential risk of MPs and chemicals.

Population structure and phylogeography of three closely related tree peonies.

Paeonia decomposita, Paeonia rotundiloba, and Paeonia rockii are three closely related species of Sect. Moutan is distributed in the montane area of the Eastern Hengduan Mountain region. Understanding the population history of these three tree peony species could contribute to unraveling the evolutionary patterns of undergrowth species in this hotspot area. We used one nuclear DNA marker (internal transcribed spacer region, ITS) and two chloroplast DNA markers (matK, ycf1) to reconstruct the phylogeographic pattern of the populations. In total, 228 individuals from 17 populations of the three species were analyzed in this study. Three nuclear clades (Clade I - Clade III) and four maternal clades (Clade A - Clade D) were reconstructed. Molecular dating suggested that young lineages diverged during the late Pliocene and early Pleistocene, younger than the uplift of the Hengduan Mountains but older than the last glacial maximum (LGM). Significant population and phylogeographic structures were detected at both markers. Furthermore, the populations of these tree peonies were overall at equilibrium during the climatic oscillations of the Pleistocene. The simulated palaeoranges of the three species during the LGM period mostly overlapped, which could have led to cross-breeding events. We propose an evolutionary scenario in which mountain orogenesis around the Hengduan Mountain area triggered parapatric isolation between maternal lineages of tree peonies. Subsequent climatic fluctuations drove migration and range recontact of these populations along the valleys. This detailed evolutionary history provides new insights into the phylogeographic pattern of species from mountain-valley systems.

An overview of bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils.

The global problem of petroleum contamination in soils seriously threatens environmental safety and human health. Current studies have successfully demonstrated the feasibility of bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils due to their easy implementation, environmental benignity, and enhanced removal efficiency compared to bioremediation. This paper reviewed recent progress and development associated with bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils. The working principles, removal efficiencies, affecting factors, and constraints of the two technologies were thoroughly summarized and discussed. The potentials, challenges, and future perspectives were also deliberated to shed light on how to overcome the barriers and realize widespread implementation on large scales of these two technologies.

Vertically stratified methane, nitrogen and sulphur cycling and coupling mechanisms in mangrove sediment microbiomes.

BACKGROUND: Mangrove ecosystems are considered as hot spots of biogeochemical cycling, yet the diversity, function and coupling mechanism of microbially driven biogeochemical cycling along the sediment depth of mangrove wetlands remain elusive. Here we investigated the vertical profile of methane (CH4), nitrogen (N) and sulphur (S) cycling genes/pathways and their potential coupling mechanisms using metagenome sequencing approaches. RESULTS: Our results showed that the metabolic pathways involved in CH4, N and S cycling were mainly shaped by pH and acid volatile sulphide (AVS) along a sediment depth, and AVS was a critical electron donor impacting mangrove sediment S oxidation and denitrification. Gene families involved in S oxidation and denitrification significantly (P < 0.05) decreased along the sediment depth and could be coupled by S-driven denitrifiers, such as Burkholderiaceae and Sulfurifustis in the surface sediment (0-15 cm). Interestingly, all S-driven denitrifier metagenome-assembled genomes (MAGs) appeared to be incomplete denitrifiers with nitrate/nitrite/nitric oxide reductases (Nar/Nir/Nor) but without nitrous oxide reductase (Nos), suggesting such sulphide-utilizing groups might be an important contributor to N2O production in the surface mangrove sediment. Gene families involved in methanogenesis and S reduction significantly (P < 0.05) increased along the sediment depth. Based on both network and MAG analyses, sulphate-reducing bacteria (SRB) might develop syntrophic relationships with anaerobic CH4 oxidizers (ANMEs) by direct electron transfer or zero-valent sulphur, which would pull forward the co-existence of methanogens and SRB in the middle and deep layer sediments. CONCLUSIONS: In addition to offering a perspective on the vertical distribution of microbially driven CH4, N and S cycling genes/pathways, this study emphasizes the important role of S-driven denitrifiers on N2O emissions and various possible coupling mechanisms of ANMEs and SRB along the mangrove sediment depth. The exploration of potential coupling mechanisms provides novel insights into future synthetic microbial community construction and analysis. This study also has important implications for predicting ecosystem functions within the context of environmental and global change. Video Abstract.

Dynamically reversible cooperation and interaction of multiple rotating micromotors.

Micromotors have been shown to have great potential in various fields (e.g., targeted therapeutics, self-organizing systems), and research on the cooperative and interactive behaviours of multiple micromotors could potentially revolutionize many fields in terms of performing multiple or complex tasks to compensate for the limitations of individual micromotors; however, dynamically reversible transitions among diverse behaviours remain much less explored, and such dynamic transformations are advantageous for achieving complex tasks. Here, we present a microsystem consisting of multiple disk-like micromotors capable of performing reversible transformations between cooperative and interactive behaviours at the liquid surface. The micromotors with aligned magnetic particles in our system have great magnet properties, which provides a strong magnetic interaction with each other and is vital for the whole microsystem. We offer and analyse the physical models among multiple micromotors concerning the cooperative and interactive modes in the lower and higher frequency ranges, respectively, between which the state transformation can reversibly occur. Furthermore, based on the proposed reversible microsystem, the feasibility of the application of self-organization is verified by demonstrating three different dynamic self-organizing behaviours. Our proposed dynamically reversible system has great potential to serve as a paradigm for studying cooperative and interactive behaviours among multiple micromotors in the future.

Soil water and nutrient availability interactively modify pollinator-mediated directional and correlational selection on floral display.

The individual and combined effects of abiotic factors on pollinator-mediated selection on floral traits are not well documented. To examine potential interactive effects of water and nutrient availability on pollinator-mediated selection on three floral display traits of Primula tibetica, we manipulated pollination and nutrient availability in a factorial experiment, conducted at two common garden sites with different soil water content (natural vs addition). We found that both water and nutrient availability affected floral trait expression in P. tibetica and that hand pollination increased seed production most when both nutrient content and water content were high, indicating joint pollen and resource limitation. We documented selection on all floral traits, and pollinators contributed to directional and correlational selection on plant height and number of flowers. Soil water and nutrient availability interactively influenced the strength of both pollinator-mediated directional and correlational selection, with significant selection observed when nutrient or water availability was high, but not when none or both were added. The results suggest that resource limitation constrains the response of P. tibetica to among-individual variation in pollen receipt, that addition of nutrients or water leads to pollinator-mediated selection and that effects of the two abiotic factors are nonadditive.

Interactive and synergistic behaviours of multiple heterogeneous microrobots.

Microrobots have been extensively studied for biomedical applications, and significant innovations and advances have been made in diverse aspects of the field. However, most studies have been based on individual microrobots with limited capabilities, constraining their scalability of functions for practical use. Here, we demonstrate the interactive and synergistic behaviours of multiple microrobots that are heterogeneous or incompletely homogeneous. A frequency-response theory is proposed where in a certain frequency range of an external rotating magnetic field (RMF), microrobots with dispersed and linearly aligned magnetic nanoparticles (MNPs) would exhibit similar and different behaviour, respectively. These microrobots rotate following the rotation of the external field, and such complete rotational motion is interrupted when the frequency exceeds a certain value, called the critical frequency (cf), but such behaviour is more prominent in microrobots with linear MNPs. Upon further investigating the effect of various parameters on the cf of the microrobots during the fabrication process, we find that heterogeneous microrobots with specific cf values can be customized. In addition, experiments and simulations are combined to show the hydrodynamic behaviours around the rotating microrobots at different frequencies. Based on these findings, the interactive and synergistic behaviours of multiple microrobots are presented, which suggests great potential for the independent execution of multiple tasks or the synergistic performance of complex tasks and is significant for the future development of interactive synergistic microrobots in the biomedical field.

Enhanced CO2 reduction and acetate synthesis in autotrophic biocathode by N-Hexanoyl-L-homoserine lactone (C6HSL)-based quorum-sensing regulation.

The aim of this study was to investigate the ecological role of quorum-sensing signaling molecule on the autotrophic biocathode for CO2 reduction and acetate synthesis. As a typical quorum-sensing signaling molecule, N-Hexanoyl-L-homoserine lactone (C6HSL) was used to regulate the construction of cathode biofilm. Results showed that the maximum acetate production from CO2 reduction improved by 94.8%, and the maximum Faraday efficiency of the microbial electrosynthesis system enhanced by 71.7%, with the regulation of C6HSL. Electrochemical analyses indicated that higher electrochemical activity and lower charge resistance of biocathode were obtained with C6HSL than without C6HSL. Confocal laser scanning microscopy and electron inhibitor experiment suggested that exogenous C6HSL increased living biomass in the biofilm and facilitated the electron transfer pathway related to NADH dehydrogenase-CoQ and proton motive force. With the C6HSL regulation, the relative abundance of hydrogen producers (e.g., Desulfovibrio and Desulfomicrobium) increased, contributing to the improved performance of autotrophic biocathode.

Stability Mechanism of Low Temperature C2H4-SCR with Activated-Carbon-Supported MnO x -Based Catalyst.

Manganese-based catalysts have shown great potential for use as a hydrocarbon reductant for NO x reduction (HC-SCR) at low temperatures if their catalytic stability could be further maintained. The effect of CeO2 as a promoter and catalyst stability agent for activated carbon supported MnO x was investigated during low temperature deNO x based on a C2H4 reductant. The modern characterization technology could provide a clear understanding of the activity observed during the deNO x tests. When reaction temperatures were greater than 180 °C and with ceria concentrations more than 5%, the overall NO conversion became stable near 70% during long duration testing. In situ DRIFTS shows that C2H4 is adsorbed on the Mn3Ce3/NAC catalysts to generate hydrocarbon activated intermediates, R-COOH, and the reaction mechanism followed the E-R mechanism. The stability and the analytical data pointed to the formation of stable oxygen vacancies within Ce3+/Ce4+ redox couplets that prevented the reduction of MnO2 to crystalline Mn2O3 and promoted the chemisorption of oxygen on the surface of MnO x -CeO x structures. Based on the data, a synergetic mechanism model of the deNO x activity is proposed for the MnO x -CeO x catalysts.