TP53AIP1 induce autophagy via the AKT/mTOR signaling pathway in the breast cancer cells.

Breast cancer ranks the first in the incidence of female cancer and is the most common cancer threatening the life and health of women worldwide.Tumor protein p53-regulated apoptosis-inducing protein 1 (TP53AIP1) is a pro-apoptotic gene downstream of p53. However, the role of TP53AIP1 in BC needs to be investigated. In vitro and in vivo experiments were conducted to assess the biological functions and associated mechanisms. Several bioinformatics analyses were made, CCK8 assay, wound healing, transwell assays, colony formation assay, EDU, flow cytometry, Immunofluorescence, qRT-PCR and Western-blotting were performed. In our study, we discovered that BC samples had low levels of TP53AIP1 expression, which correlated with a lower survival rate in BC patients. When TP53AIP1 was up-regulated, it caused a decrease in cell proliferation, migration, and invasion. It also induced epithelial-to-mesenchymal transition (EMT) and protective autophagy. Furthermore, the over-expression of TP53AIP1 suppressed tumor growth when tested in vivo. We also noticed that TP53AIP1 up-regulation resulted in decreased levels of phosphorylation in AKT and mTOR, suggesting a mechanistic role. In addition, we performed functional rescue experiments where the activation of AKT was able to counteract the impact of TP53AIP1 on the survival and autophagy in breast cancer cell lines. This suggests that TP53AIP1 acts as an oncogene by controlling the AKT/mTOR pathway. These findings reveal TP53AIP1 as a gene that suppresses tumor growth and triggers autophagy through the AKT/mTOR pathway in breast cancer cells. As a result, TP53AIP1 presents itself as a potential target for novel therapeutic approaches in treating breast cancer.

Independent ultrahigh-Q dual-band resonances via coating a resonator on a BIC-driven metasurface.

Traditional designs driven by symmetry-protected bound states in the continuum (SP-BICs) hardly support independent dual-band resonances, and they require extremely small perturbations to obtain an ultrahigh-Q. Here, we propose an SP-BIC-driven structure composed of a metasurface and a resonator, which supports independent dual-band resonances and enables ultrahigh-Q at large perturbations. The underlying mechanism enabling this is to form reasonable eigenfield distributions of two BICs by coating a dielectric layer on the metasurface. One eigenfield is confined within the metasurface and the bottom of the resonator, while the other one concentrates at the top of the resonator. Thus, two resonances with different originations can be supported, and the effect of metasurface perturbations on the eigenfields is weakened. This work provides a promising pathway for unlocking the potential of SP-BICs, enhancing light trapping and manipulation across diverse applications.

Thermal-tagging photoacoustic remote sensing flowmetry.

Ultrasound coupling is one of the critical challenges for traditional photoacoustic (or optoacoustic) microscopy (PAM) techniques transferred to the clinical examination of chronic wounds and open tissues. A promising alternative potential solution for breaking the limitation of ultrasound coupling in PAM is photoacoustic remote sensing (PARS), which implements all-optical non-interferometric photoacoustic measurements. Functional imaging of PARS microscopy was demonstrated from the aspects of histopathology and oxygen metabolism, while its performance in hemodynamic quantification remains unexplored. In this Letter, we present an all-optical thermal-tagging flowmetry approach for PARS microscopy and demonstrate it with comprehensive mathematical modeling and ex vivo and in vivo experimental validations. Experimental results demonstrated that the detectable range of the blood flow rate was from 0 to 12 mm/s with a high accuracy (measurement error:±1.2%) at 10-kHz laser pulse repetition rate. The proposed all-optical thermal-tagging flowmetry offers an effective alternative approach for PARS microscopy realizing non-contact dye-free hemodynamic imaging.

Widefield functional speckle-correlation optical scattering mesoscopy toward hemodynamic imaging.

Speckle-correlation optical scattering imaging (SCOSI) has shown the potential for non-invasive biomedical diagnostic applications, which directly utilizes the scattering patterns to reconstruct the deep and non-line-of-sight objects. However, the course of the translation of this technique to preclinical biomedical imaging applications has been postponed by the following two facts: 1) the field of view of SCOSI was significantly limited by the optical memory effect, and 2) the molecular-tagged functional imaging of the biological tissues remains largely unexplored. In this work, a proof-of-concept design of the first-generation widefield functional SCOSI (WF-SCOSI) system was presented for simultaneously achieving mesoscopic mapping of fluid morphology and flow rate, which was realized by implementing the concepts of scanning synthesis and fluorescence scattering flowmetry. The ex vivo imaging results of the fluorescence-labeled large-scale blood vessel network phantom underneath the strong scatters demonstrated the effectiveness of WF-SCOSI toward non-invasive hemodynamic imaging applications.

Fast autofocusing of recorded planes by salient feature region for coherent diffraction imaging.

In multi-distance coherent diffraction imaging, the task of distance calculation for multi-diffraction images is cumbersome. The information features are hard-to-extract and the region of interest extraction algorithms are difficult to be adopted. A universal salient feature region selection algorithm by using the area with the highest density of corners is proposed to extract the most representative feature region. In addition, equally spaced recording modes and mismatched diffraction distances will result in system noise and destroy image quality. The polydirectional maximum gradient is offered as a sharpness criterion to weigh a quantitative feature for the final pattern. A fast, sensitive, and high-accuracy autofocusing and sample reconstruction can be achieved using only a small number of images while ensuring that morphological properties and quantification of the reconstructions are not compromised. The proposed method is promising for biological and medical dynamic observations for computational imaging systems.

Effects of castration and eucalyptus oil supplementation on growth performance, nutrient digestibility, and blood-immunity indicators of male Holstein calves.

The purpose of this study was to investigate the effects of early castration and eucalyptus oil (EUC) supplementation on dry matter intake (DMI), growth performance, and immune response of Holstein calves. Fifty-six male Holstein calves 52 d old and with an initial body weight (BW) of 63.5 ± 5.27 kg were used. The animals were blocked by BW and randomly assigned into 1 of the 4 treatment groups in a randomized complete block design with a 2 (no castration vs. castration) × 2 (without vs. with EUC) factorial arrangement of treatments. The treatments were (1) uncastrated calves fed without EUC, (2) uncastrated calves fed 0.5 g/d EUC (EUC group), (3) castrated calves (steers) fed without EUC (castrated group), and (4) steers fed with 0.5 g/d EUC (castrated + EUC). The experiment was 8 wk long, including pre- and postweaning (weaned at 72 d). The EUC × castrated interactions were not significant for DMI, growth performance, nutrient digestibility, and immune response. Castration did not affect the DMI, final BW, average daily gain (ADG), or feed efficiency, except that the ADG was greater for bull calves than for steers at postweaning. Supplementation with EUC increased DMI pre- and postweaning and increased the ADG of weaned calves. Digestibility in the total digestive tract was not affected by castration (except for organic matter digestibility), whereas adding EUC improved the digestibility of dry matter, acid detergent fiber, and crude protein. Blood concentration of IL-6 at d 94 was decreased by feeding EUC. These results indicate that the EUC could be fed to either intact or castrated dairy calves to promote growth and health postweaning; castration before weaning may reduce ADG and cause inflammatory stress without affecting feed intake or feed efficiency.

RETRACTED: Liu et al. The Effect of Intrinsic Mechanical Properties on Reducing the Friction-Induced Ripples of Hard-Filler-Modified HDPE. Polymers 2023, 15, 268.

The Polymers Editorial Office retracts the article "The Effect of Intrinsic Mechanical Properties on Reducing the Friction-Induced Ripples of Hard-Filler-Modified HDPE" [...].

Modeling and Analysis of a Conical Bridge-Type Displacement Amplification Mechanism Using the Non-Uniform Rational B-Spline Curve.

This paper presents a new analytical model of a conical bridge-type displacement amplification mechanism (DAM) considering the effect of external loads and a piezostack actuator (PSA). With the merits of simple implementation and better fitting, the non-uniform rational B-spline (NURBS) is employed to parameterize conical connecting beams of the DAM, and an analytical model of the displacement amplification ratio and input stiffness is established based on Castigliano's second theorem. After that, considering the interactions with elastic loads and PSA, the actual displacement amplification ratio of the conical DAM is obtained, and the effect of the shape of connecting beams in the performance of the DAM is further analyzed. The proposed analytical model is verified by finite element analysis (FEA), and the results show a maximum error of 6.31% between the calculated value and FEA results, demonstrating the accuracy of the proposed model. A prototype of the conical DAM with optimized shape is fabricated and experimentally tested, which further validates the effectiveness and accuracy of the proposed analytical model. The proposed model offers a new method for analysis and shape optimization of the bridge-type DAM under specific elastic loads.

Fano resonance in a microring resonator with a micro-reflective unit.

Fano resonance is considered to be a promising approach for integrated sensing. However, achieving and controlling Fano resonance lineshapes on ultra-compact chips remains a challenge. In this article, we propose a theoretic model based on the transfer matrix method (TMM) to quantitatively interpret the impact of a micro-reflective unit (MRU) etched in the straight waveguide of a microring resonator (MRR). Numerical calculations and FDTD simulations indicate that the size and position of the MRU can be used to control the Fano resonance lineshape. Since the MRU is etched in the coupling region, the reflection caused by the MRU will significantly enhance the intensity of the counter-clockwise (CCW) mode in the microring. When applied to a single nanoparticle sensing, clockwise (CW) and CCW modes will couple due to a single nanoparticles or rough cavity walls, resulting in a sharp shift and split of the Fano lineshape. The proposed model for single nanoparticle sensing is described by the scattering matrix, and the calculations show a well matches with FDTD simulations. The results show that the model proposed in this paper provides a new theoretical basis for controlling Fano resonance lineshape and presents a new approach for the integrated sensing of silicon photonic devices with high sensitivity.

Comparison of Chemical Compositions and Antioxidant Activity of Essential Oils from Litsea Cubeba, Cinnamon, Anise, and Eucalyptus.

The purpose of this study was to compare the antioxidant activity of litsea cubeba oil (LCO), cinnamon oil (CO), anise oil (AO), and eucalyptus oil (EUC) in vitro. The chemical compositions of the essential oils (EOs) were analyzed using gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of the four EOs was evaluated through scavenging DPPH free radicals, chelating Fe2+, scavenging hydroxyl free radicals, and inhibiting yolk lipid peroxidation. The results showed that the major compounds found in LCO, CO, AO, and EUC are citral (64.29%), cinnamaldehyde (84.25%), anethole (78.51%), and 1,8-cineole (81.78%), respectively. The four EOs all had certain antioxidant activity. The ability to scavenge DPPH radical was ranked in the order of LCO > CO > AO > EUC. The hydroxyl radical scavenging ability was ranked in the order of EUC > CO > LCO > AO. The chelating Fe2+ capacity was ranked in the order of EUC > AO > CO > LCO. The yolk lipid peroxidation inhibition ability was ranked in the order of CO > AO > EUC > LCO. In different antioxidant activity assays, the antioxidant activity of the EOs was different. It was speculated that the total antioxidant activity of an EO may be the result of the joint action of different antioxidant capacities.

Protective Effect of Citrus Medica limonum Essential Oil against Escherichia coli K99-Induced Intestinal Barrier Injury in Mice.

Citrus Medica limonum essential oil (LEO) has been reported to have antibacterial and anti-inflammatory activities, but its protective effect in the intestine remains unknown. In this study, we researched the protective effects of LEO in relation to intestinal inflammation induced by E. coli K99. The mice were pretreated with 300, 600, and 1200 mg/kg LEO and then stimulated with E. coli K99. The results showed that E. coli K99 caused immune organ responses, intestinal tissue injury, and inflammation. LEO pretreatment dose-dependently alleviated these changes by maintaining a low index in the thymus and spleen and producing a high content of immunoglobulin A, G, and M (IgA, IgG, and IgM) and low content of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and interleukin-6 (IL-6). Intestinal integrity as a consequence of the LEO pretreatment may be related to the high mRNA expression of intestinal trefoil factor (ITF) and the low mRNA expression of transforming growth factor-ß1 (TGF-ß1). Conclusively, an LEO pretreatment can alleviate E. coli K99-induced diarrhea, immune organ response, and body inflammation in mice by reducing the levels of inflammatory cytokines and improving the levels of immunoglobulin, and the intestinal integrity remained highest when maintaining the high mRNA expression of ITF and keeping the mRNA expression of TGF-ß1 low in the intestinal tissue.

The acoustic performances of a subwavelength hierarchical honeycomb structure: Analytical, numerical, and experimental investigations.

This paper proposes a subwavelength hierarchical honeycomb structure (SHHS) with a compact lateral dimension and double-band perfect absorption in low frequencies. Unlike the conventional micro-perforated panel (MPP)-honeycomb sandwich absorber, this structure has an additional internal honeycomb with a perforated wall. Therefore, there are two resonant cavities in the SHHS to realize multiple absorption peaks. Analytical, numerical, and experimental investigations are performed to study the proposed system's acoustic performance in absorption. The SSHS is simplified into four parts and its analytical model is constructed by combining various analytical models by acoustic-electro analogy. The analytical model is presented to explore the physical properties of sound absorption and the influence of parameters, which has been validated by comparisons with the numerical model, and the experimental data is measured by an impedance tube. It is found that the main incident energy is lost by the inside hole, which is different from the conventional absorbers with surface MPP. Moreover, the side length of the internal honeycomb can adjust the resonant frequencies to achieve an absorber with the subwavelength. A SSHS is designed with a perfect absorption at 320 Hz whose thickness is 1/31 of the resonant frequency wavelength. The SHHS has excellent potential for noise control engineering applications.

The Effect of Intrinsic Mechanical Properties on Reducing the Friction-Induced Ripples of Hard-Filler-Modified HDPE.

Ripple deformations induced by friction on polymeric materials have negative effects on the entire stability of operating machineries. These deformations are formed as a response to contacting mechanics, caused by the intrinsic mechanical properties. High-density polyethylene (HDPE) with varying silicon nitride (Si3N4) contents is used to investigate different ripple deformation responses by conducting single-asperity scratch tests. The relationship between the intrinsic mechanical properties and the ripple deformations caused by filler modifications is analyzed in this paper. The results show the coupling of the inherent mechanical properties, and the stick-slip motion of HDPE creates ripple deformations during scratching. The addition of the Si3N4 filler changes the frictional response; the filler weakens the ripples and almost smoothens the scratch, particularly at 4 wt.%, but the continued increase in the Si3N4 content produces noticeable ripples and fluctuations. These notable differences can be attributed to the yield and post-yield responses; the high yield stress and strain-hardening at 4 wt.% provide good friction resistance and stress distribution, thus a smooth scratch is observed. In contrast, increasing the filler content weakens both the yield and post-yield responses, leading to deformation. The results herein reveal the mechanism behind the initial ripple deformation, thus providing fundamental insights into universally derived friction-induced ripples.

Spatial Distribution and Estimation Model of Soil pH in Coastal Eastern China.

Soil pH is an essential indicator for assessing soil quality and soil health. In this study, based on the Chinese farmland soil survey dataset and meteorological dataset, the spatial distribution characteristics of soil pH in coastal eastern China were analyzed using kriging interpolation. The relationships between hydrothermal conditions and soil pH were explored using regression analysis with mean annual precipitation (MAP), mean annual temperature (MAT), the ratio of precipitation to temperature (P/T), and the product of precipitation and temperature (P*T) as the main explanatory variables. Based on this, a model that can rapidly estimate soil pH was established. The results showed that: (a) The spatial heterogeneity of soil pH in coastal eastern China was obvious, with the values gradually decreasing from north to south, ranging from 4.5 to 8.5; (b) soil pH was significantly correlated with all explanatory variables at the 0.01 level. In general, MAP was the main factor affecting soil pH (r = -0.7244), followed by P/T (r = -0.6007). In the regions with MAP < 800 mm, soil pH was negatively correlated with MAP (r = -0.4631) and P/T (r = -0.7041), respectively, and positively correlated with MAT (r = 0.6093) and P*T (r = 0.3951), respectively. In the regions with MAP > 800 mm, soil pH was negatively correlated with MAP (r = -0.6651), MAT (r = -0.5047), P/T (r = -0.3268), and P*T (r = -0.5808), respectively. (c) The estimation model of soil pH was: y = 23.4572 - 6.3930 × lgMAP + 0.1312 × MAT. It has been verified to have a high accuracy (r = 0.7743, p < 0.01). The mean error, the mean absolute error, and the root mean square error were 0.0450, 0.5300, and 0.7193, respectively. It provides a new path for rapid estimation of the regional soil pH, which is important for improving the management of agricultural production and slowing down soil degradation.

The Chemical Composition and Antibacterial and Antioxidant Activities of Five Citrus Essential Oils.

Increasing concerns over the use of antimicrobial growth promoters in animal production has prompted the need to explore the use of natural alternatives such as phytogenic compounds and probiotics. Citrus EOs have the potential to be used as an alternative to antibiotics in animals. The purpose of this research was to study the antibacterial and antioxidant activities of five citrus EOs, grapefruit essential oil (GEO), sweet orange EO (SEO), bergamot EO (BEO), lemon EO (LEO) and their active component d-limonene EO (DLEO). The chemical composition of EOs was analyzed by gas chromatography-mass spectrometry (GC-MS). The antibacterial activities of the EOs on three bacteria (Escherichia coli, Salmonella and Lactobacillus acidophilus) were tested by measuring the minimum inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and inhibition zone diameter (IZD). The antioxidant activities of EOs were evaluated by measuring the free radical scavenging activities of DPPH and ABTS. We found that the active components of the five citrus EOs were mainly terpenes, and the content of d-limonene was the highest. The antibacterial test showed that citrus EOs had selective antibacterial activity, and the LEO had the best selective antibacterial activity. Similarly, the LEO had the best scavenging ability for DPPH radicals, and DLEO had the best scavenging ability for ABTS. Although the main compound of the five citrus EOs was d-limonene, the selective antibacterial and antioxidant activity of them might not be primarily attributed to the d-limonene, but some other compounds' combined action.

Random motion blur for optical image encryption.

We present a compact optical encryption scheme by using a continuous-random-motion blurring model in an optical imaging system. Image encryption is performed by additive motion blur effects with continuous and random shifts of a camera. Real-time random phase modulation can be achieved without the use of random phase mask. Storage of the key is more convenient, which only requires parameters of motion. In addition, modulation characteristics are different from the traditional encryption schemes. On the premise of high security, modulation space is broadened, and flexibility of encryption is further improved. Simulations and experiments verify the validity of the motion blur-based crypto-system and demonstrate its security under several attacks. This novel method will be significant for the practical applications in the field of optical information security.

Radially polarized cosine non-uniformly correlated beams and their propagation properties.

We introduce a kind of radially polarized partially coherent (RPPC) beam with a prescribed non-uniform correlation function, called a radially polarized cosine non-uniformly correlated (RPCNUC) beam. Based on the extended Huygens-Fresnel principle, we study the propagation properties in free space and in a turbulent atmosphere. Unlike RPPC beams with uniform coherence, RPCNUC beams possess the invariance of dark hollow cores and radial polarization, and exhibit self-focusing properties. In a turbulent atmosphere, the intensity distribution demonstrates self-healing properties over a certain propagation distance. We also investigate how to adjust the beam parameters to reduce the turbulence-induced degradation in detail.

Fast autofocusing based on pixel difference with the Tanimoto coefficient between images.

Focusing objects accurately over short time scales is an essential and nontrivial task for a variety of microscopy applications. In this Letter, an autofocusing algorithm using pixel difference with the Tanimoto coefficient (PDTC) is described to predict the focus. Our method can robustly distinguish differences in clarity among datasets. The generated auto-focusing curves have extremely high sensitivity. A dataset of a defocused stack acquired by an Olympus microscope demonstrates the feasibility of our technique. This work can be applied in full-color microscopic imaging systems and is also valid for single-color imaging.

Optically reconfigurable higher-order valley photonic crystals based on enhanced Kerr effect.

The reconfigurable higher-order topological states are realized in valley photonic crystals with enhanced optical Kerr nonlinearity. The inversion symmetry of the designed valley photonic crystal is broken due to the difference in optical responses between adjacent elements rather than their geometry structures. Therefore, by constructing photonic crystals with distinct topological phases, valley-dependent topological states can be realized, and their reconfigurability is demonstrated based on the Kerr effect. The investigated higher-order topological photonic crystals exhibit great robustness against the structural defects and inferior quality of pump introduced around the corner. Our work provides a new, to the best of our knowledge, platform for studying optical field manipulation and optical devices fabrication in the context of nonlinear higher-order topology.

Experimental Research on Fatigue Crack Growth Behavior of Diffusion-Bonded Titanium Alloy Laminates with Preset Unbonded Areas.

This paper aimed to study the fatigue crack growth behavior of diffusion-bonded titanium alloy laminates (DB-TAL) with preset unbonded areas using an experimental method to understand the toughening mechanisms of presetting unbonded areas in DB-TAL. For two series of specimens of DB-TAL with preset unbonded areas with an open hole, which have a pre-notch at the open hole edge, fatigue experiments under tension-tension cyclic loading were conducted. The fatigue crack growth process, the crack growth rate, and the stress intensity factor on the crack front were analyzed. The results showed that the preset unbonded area leads the crack away from the stress concentration zone and slows down the crack growth rate. Therefore, the preset unbonded area significantly improved the fracture property of DB-TAL.