Characterization and genome sequence of N,N-dimethylformamide degradation in Paracoccus sulfuroxidans DM175A1-1 isolated from activated sludge of wastewater.

N,N-dimethylformamide (DMF) is an organic solvent with stable chemical properties and high boiling point. Based on its good solubility, DMF is widely used in synthetic textile, leather, electronics, pharmaceutical and pesticide industries. However, the DMF pollutes the environment and does harm to human liver function, kidney function, and nerve function. Herein, an efficient DMF-degrading strain, DM175A1-1, was isolated and identified as Paracoccus sulfuroxidans. This strain can use DMF as the sole source of carbon and nitrogen. Whole-genome sequencing of strain DM175A1-1 revealed that it has a 3.99-Mbp chromosome a 120-kbp plasmid1 and a 40-kbp plasmid2. The chromosome specifically harbors the dmfA1 and dmfA2 essential for the initial steps of DMF degradation. And it also carries the some part of genes facilitating subsequent methylotrophic metabolism and glutathione-dependent pathway. Through further DMF tolerance degradation experiments, DM175A1-1 can tolerate DMF concentrations up to 10,000 mg/L, whereas the majority of Paracoccus strains could only show degradation activity below 1,000 mg/L. And the efficiency of organic nitrogen conversion to NH3-N in DMF can reach 99.0% when the hydraulic retention time (HRT) is controlled at 5 days. Meanwhile, it showed a significant degradation effect at a pharmaceutical enterprise in Zhejiang Province with high concentration of DMF wastewater. This study provides a new strain Paracoccus sulfuroxidans DM175A1-1 which shows a significant influence on DMF degradation, and reveals the characterization on its DMF degradation. It lays the foundation for the application of biological method in the efficient degradation of DMF in industrial wastewater.

Two new species of the mealybug genus Paracoccus from Jiangxi, South China (Hemiptera, Coccomorpha, Pseudococcidae).

Two new mealybug species, Paracoccusgillianwatsonae Zhang, sp. nov. and P.wui Zhang, sp. nov., collected from Jiangxi, South China, are described and illustrated based on the morphology of adult females. Paracoccusgillianwatsonae is similar to P.burnerae (Brain, 1915), but it differs in having fewer pairs of cerarii, and in lacking both ventral oral collar tubular ducts on the margins of the head and translucent pores on the hind femur. Paracoccuswui resembles P.keralae Williams, 2004 and P.neocarens (Lit, 1992), but it differs in lacking ventral oral collar tubular ducts on the margins of the head and in having multilocular disc-pores usually in double rows at the posterior edges of abdominal segments V and VI. A key to the Paracoccus species found in China is provided.

First Principles Investigation of C, Cl2 and CO Co-Adsorption on ZrSiO4 Surfaces for Carbochlorination Reaction.

The study of the adsorption behavior of C, CO and Cl2 on the surface of ZrSiO4 is of great significance for the formulation of the technological parameters in the carbochlorination reaction process. Based on first principles, the adsorption structure, adsorption energy, Barder charge, differential charge density, partial density of states and energy barrier were calculated to research the adsorption and reaction mechanism of C and Cl2 on ZrSiO4 surfaces. The results indicated that when C, CO and Cl2 co-adsorbed on the surface of ZrSiO4, they interacted with surface atoms and the charge transfer occurred. The Cl2 molecules dissociated and formed Zr-Cl bonds, while C atoms formed C1=O1 bonds with O atoms. Compared with CO, the co-adsorption energy and reaction energy barrier of C and Cl2 are lower, and the higher the C content, the lower the adsorption energy and energy barrier, which is beneficial for promoting charge transfer and the dissociation of Cl2. The 110-2C-2Cl2 has the lowest adsorption energy and the highest reaction activity, with adsorption energy and energy barriers of -13.45 eV and 0.02 eV. The electrons released by C are 2.30 e, while the electrons accepted by Cl2 are 2.37 e.

FBXO24 modulates mRNA alternative splicing and MIWI degradation and is required for normal sperm formation and male fertility.

Spermiogenesis is a critical, post-meiotic phase of male gametogenesis, in which the proper gene expression is essential for sperm maturation. However, the underFlying molecular mechanism that controls mRNA expression in the round spermatids remains elusive. Here, we identify that FBXO24, an orphan F-box protein, is highly expressed in the testis of humans and mice and interacts with the splicing factors (SRSF2, SRSF3, and SRSF9) to modulate the gene alternative splicing in the round spermatids. Genetic mutation of FBXO24 in mice causes many abnormal splicing events in round spermatids, thus affecting a large number of critical genes related to sperm formation that were dysregulated. Further molecular and phenotypical analyses revealed that FBXO24 deficiency results in aberrant histone retention, incomplete axonemes, oversized chromatoid body, and abnormal mitochondrial coiling along sperm flagella, ultimately leading to male sterility. In addition, we discovered that FBXO24 interacts with MIWI and SCF subunits and mediates the degradation of MIWI via K48-linked polyubiquitination. Furthermore, we show that FBXO24 depletion could lead to aberrant piRNA production in testes, which suggests FBXO24 is required for normal piRNA counts. Collectively, these data demonstrate that FBXO24 is essential for sperm formation by regulating mRNA alternative splicing and MIWI degradation during spermiogenesis.

Factors Influencing Copulation Duration in Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae).

The gregarious ectoparasitic beetle Dastarcus helophoroides (Fairmaire) is considered a primary biocontrol agent for controlling several cerambycid pests in East Asian countries. A thorough study of reproductive behavior is a prerequisite for the mass production of natural insect predators. Nonetheless, little attention has been given to this ectoparasitic beetle. We performed a series of trials to assess whether the adult copulation duration, a key behavioral trait, is differentially influenced by physiological and ecological factors, including body size, mating history, kinship, sex ratio, mating sequence, feeding status, ambient temperature, photoperiod, and time of day. Additionally, the effect of the copulation duration on the reproductive output of this beetle was also investigated. The results indicated that the copulation duration varied considerably, ranging from 1.12 min to 16.40 min and lasting for an average of 9.11 ± 0.12 min. Females with longer copulations laid more eggs and had a greater proportion of eggs hatched. Medium-sized individuals copulated significantly longer than small- and large-sized individuals. The copulation durations were significantly longer when both sexes experienced an asymmetric mating history than when both sexes experienced a symmetric mating history. Inbred couples copulated significantly longer than outbred couples. In terms of the adult sex ratio, increasing the density of females (polygamous group) or males (polyandrous group) led to significantly longer copulation durations than those in the monogamous group. The copulation durations gradually decreased with increasing the mating sequence and temperature. Food-absence couples copulated significantly longer than food-presence couples. The mean copulation duration of the scotophase was significantly longer than that of the photophase. These results demonstrate that all of the analyzed factors emerge as important factors influencing the copulation duration, ultimately affecting the reproductive outputs in this ectoparasitic beetle.

A metal-organic framework nanocomposite with oxidation and near-infrared light cascade response for bacterial photothermal inactivation.

Photothermal treatment is an effective and precise bacterial disinfection method that can reduce the occurrence of bacterial drug resistance. However, most conventional photothermal treatment strategies have the problem that the photothermal response range does not match the infection area. Herein, a metal-organic framework (MOF) nanocomposite responding to the oxidation state of the bacterial infection microenvironment was constructed for near-infrared (NIR) photothermal bacterial inactivation. In this strategy, the MOF was used as a nanocarrier to load tetramethylbenzidine (TMB) and horseradish peroxidase (HPR). The high oxidation state of the bacterial infection microenvironment can trigger the enzyme-catalyzed reaction of the nanocomposite, thereby generating oxidation products with the NIR photothermal effect for bacterial disinfection. The synthesis and characterization of the nanocomposite, oxidation state (H2O2) response effect, photothermal properties, and antibacterial activities were systematically studied. This study provides a new idea for building a precision treatment system for bacterial infection.

Regulatory roles of alternative splicing at Ezh2 gene in mouse oocytes.

BACKGROUND: Enhancer of zeste homologue 2 (EZH2), the core member of polycomb repressive complex 2 (PRC2), has multiple splicing modes and performs various physiological functions. However, function and mechanism of alternative splicing at Ezh2 exon 3 in reproduction are unknown. METHODS: We generated Ezh2Long and Ezh2Short mouse models with different point mutations at the Ezh2 exon 3 alternative splicing site, and each mutant mouse model expressed either the long or the short isoform of Ezh2. We examined mutant mouse fertility and oocyte development to assess the function of Ezh2 alternative splicing at exon 3 in the reproductive system. RESULTS: We found that Ezh2Long female mice had normal fertility. However, Ezh2Short female mice had significantly decreased fertility and obstructed oogenesis, with compromised mitochondrial function in Ezh2Short oocytes. Interestingly, increased EZH2 protein abundance and accumulated H3K27me3 were observed in Ezh2Short oocytes. CONCLUSIONS: Our results demonstrate that correct Ezh2 alternative splicing at exon 3 is important for mouse oogenesis.

H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis.

Spermatogenesis is precisely controlled by sophisticated gene expression programs and is driven by epigenetic reprogramming, including histone modification alterations and histone-to-protamine transition. Nuclear receptor binding SET domain protein 2 (Nsd2) is the predominant histone methyltransferase catalyzing H3K36me2 and its role in male germ cell development remains elusive. Here, we report that NSD2 protein is abundant in spermatogenic cells. Conditional loss of Nsd2 in postnatal germ cells impaired fertility owing to apoptosis of spermatocytes and aberrant spermiogenesis. Nsd2 deficiency results in dysregulation of thousands of genes and remarkable reduction of both H3K36me2 and H3K36me3 in spermatogenic cells, with H3K36me2 occupancy correlating positively with expression of germline genes. Nsd2 deficiency leads to H4K16ac elevation in spermatogenic cells, probably through interaction between NSD2 and PSMA8, which regulates acetylated histone degradation. We further reveal that Nsd2 deficiency impairs EP300-induced H4K5/8ac, recognized by BRDT to mediate the eviction of histones. Accordingly, histones are largely retained in Nsd2-deficient spermatozoa. In addition, Nsd2 deficiency enhances expression of protamine genes, leading to increased protamine proteins in Nsd2-deficient spermatozoa. Our findings thus reveal a previously unappreciated role of the Nsd2-dependent chromatin remodeling during spermatogenesis and provide clues to the molecular mechanisms in epigenetic abnormalities impacting male reproductive health.

A multi-year dormancy strategy in a cabbage beetle population in southeastern China.

The life cycle of the cabbage beetle Colaphellus bowringi in southeastern China is complex due to four options for adult development: summer diapause, winter diapause, prolonged diapsuse, and nondiapause. However, detailed information on the multi-year emergence patterns of diapausing individuals in this beetle has not been documented. In this study, we monitored the adult emergence patterns of diapausing individuals and estimated the influence of the diapause-inducing temperature and photoperiod on the incidence of prolonged diapause under seminatural conditions for several years. The duration of diapause for adults collected from the vegetable fields in different years varied from several months to 5 years. Approximately 25.9%-29.2% of individuals showed prolonged diapause (emergence more than 1 year after entering diapause) over the 5 years of observation. Furthermore, regardless of insect age, the emergence of diapausing adults from the soil always occurred between mid-February and March in spring and between late August and mid-October in autumn, when the host plants were available. The influence of diapause-inducing temperatures (22, 25, and 28°C) combined with different photoperiods (L:D 12:12 h and L:D 14:10 h) on diapause duration was tested under seminatural conditions. Pairwise comparisons of diapause duration performed by the log-rank test revealed that the low temperature of 22°C combined with the long photoperiod of L:D 14:10 h induced the longest diapause duration, whereas the low temperature of 22°C combined with the short photoperiod of L:D 12:12 h induced the highest proportion of prolonged diapause. This study indicates that C. bowringi adopts a multi-year dormancy strategy to survive local environmental conditions and unpredictable risks.

Phase-Amplitude Coupling Brain Networks in Children with Attention-Deficit/Hyperactivity Disorder.

In cognitive neuroscience, there is an increasing interest in identifying and understanding the synchronization of distinct neural oscillations with different frequencies that might support dynamic communication within the brain. This study explored the cross-frequency phase-amplitude coupling brain network characteristics of resting-state electroencephalograms between 30 children with attention-deficit/hyperactivity disorder (ADHD) and 30 age-matched typically developing children. Compared with control group, children with ADHD show increased coupling intensity and altered distribution patterns of dominant paired channels, especially in the δ-γH, θ-γH, α-γH, ßL-γH, and ßH-γH coupling networks. Regarding graph theory properties, the characteristic path length, the mean clustering coefficient, the global efficiency, and the mean local efficiency significant difference in many cross-frequency coupling networks, especially in the δ-γH, θ-γH, α-γH, ßL-γH, and ßH-γH coupling networks. The area under the receiver operating characteristic curve (AUC) in low-frequency coupling with a high-gamma frequency was larger than that in coupling with low-gamma frequency (AUC values of δ-γL, θ-γL, α-γL, ßL-γL, ßH-γL, δ-γH, θ-γH, α-γH, ßL-γH, and ßH-γH were 0.794, 0.722, 0.666, 0.570, 0.881, 0.992, 0.998, 0.998, 0.989, and 0.974, respectively). These findings demonstrate altered coupling intensity and disrupted topological organization of coupling networks, support the altered brain network theory in children with ADHD. The coupling intensity and graph theory properties of low-frequency coupling with high-gamma frequency were promising resting-state electroencephalogram biomarkers of ADHD in children.

Phototactic Behavioral Response of the Ectoparasitoid Beetle Dastarcus helophoroides (Coleoptera: Bothrideridae): Evidence for Attraction by Near-Infrared Light.

The ectoparasitoid beetle, Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae), has been widely used as a biological control agent for many cerambycid beetles in China, Korea, and Japan. However, much less is known about its phototactic behavior to visual stimuli. In this paper, the phototactic behavioral response of D. helophoroides to 27 monochromatic lights and to nine illumination intensities of the most attractive light was evaluated using light-emitting diodes (LEDs) as light sources. The results showed that in dual choice between darkness and individual LED light, D. helophoroides adults exhibited a positively phototactic response to all wavelengths ranging from 300 to 860 nm. The near-infrared light (NIR, ranging from 700 to 760 nm) elicited stronger phototactic behavioral response, showing a preference for NIR wavelengths light. In paired choice among four preferred NIR lights, D. helophoroides adults displayed a significantly more favorable response to NIR light at 700 nm. Furthermore, the beetles expressed varying levels of sensitivity to illumination intensities from 1 to 600 lux under NIR light at 700 nm. The phototactic response was strongest at 7 lux and a statistically significant downward trend was found with increasing or decreasing the illumination intensities. These findings clearly demonstrate that D. helophoroides is a positively phototactic insect and its phototactic behavior is significantly influenced by light wavelength and illumination intensity, among which NIR light with peak wavelength at 700 nm and an intensity at 7 lux are most suitable in attracting D. helophoroides adults.

H3.3 kinetics predicts chromatin compaction status of parental genomes in early embryos.

BACKGROUND: After fertilization, the fusion of gametes results in the formation of totipotent zygote. During sperm-egg fusion, maternal factors participate in parental chromatin remodeling. H3.3 is a histone H3 variant that plays essential roles in mouse embryogenesis. METHODS: Here, we used transgenic early embryos expressing H3.3-eGFP or H2B-mCherry to elucidate changes of histone mobility. RESULTS: We used FRAP analysis to identify that maternally stored H3.3 has a more significant change than H2B during maternal-to-embryonic transition. We also found that H3.3 mobile fraction, which may be regulated by de novo H3.3 incorporation, reflects chromatin compaction of parental genomes in GV oocytes and early embryos. CONCLUSIONS: Our results show that H3.3 kinetics in GV oocytes and early embryos is highly correlated with chromatin compaction status of parental genomes, indicating critical roles of H3.3 in higher-order chromatin organization.

A Study on Resting EEG Effective Connectivity Difference before and after Neurofeedback for Children with ADHD.

Altered functional networks in attention deficit/hyperactivity disorder (ADHD) have been frequently reported, but effective connectivity has hardly been studied. Especially the differences of effective connectivity in children with ADHD after receiving neurofeedback (NF) training have been merely reported. Therefore, this study aimed to explore the effective networks of ADHD and the positive influence of NF on the effective networks. Electroencephalogram (EEG) data were recorded from 22 children with ADHD (including data from children pretraining and posttraining) and 15 age-matched healthy controls during an eyes-closed resting state. Phase transfer entropy (PTE) was used to construct the effective connectivity. The topological properties of networks and flow gain were measured separately in four bands (delta, theta, alpha, and beta). Results revealed the following: pretraining children with ADHD manifested a higher clustering coefficient and lower characteristic path length in the delta band than healthy controls; weakened anterior-to-posterior flow gain in the delta band, strengthened posterior-to-anterior flow gain in the alpha band and strengthened anterior-to-posterior flow gain in the beta band were observed in pretraining children with ADHD; The topological properties and flow gain in posttraining children with ADHD were close to those of healthy controls. Moreover, parent's SWAN presented significant improvements of ADHD symptoms after NF. Our findings revealed that the effective connectivity of ADHD was altered and that NF could improve the brain function of ADHD. The present study provided the first evidence that children with ADHD differed from healthy children in phase-based effective connectivity and that NF could reduce the differences.

Electrostatic reaction for the detection of circulating tumor cells as a potential diagnostic biomarker for metastasis in solid tumor.

The detection of circulating tumor cells (CTCs) from blood samples is important to predict metastatic spread of cancer cells. However, effective quantification and identification of CTCs in solid tumors remain a challenge. Aerobic glycolysis is a hallmark of cancer cells, which makes cancer cells have more negative membrane potentials than that of normal cells. Herein, we reported a CTC isolation method with 80.7% capture efficiency based on electrostatic reaction, which was accomplished within 30 min in mimic clinical samples. Following in vitro verification using Lewis lung carcinoma (LLC1) (EpCAM-positive) and B16F10 (EpCAM-negative) melanoma cells, syngeneic tumor models were used to evaluate specificity and sensitivity of the surface charged nanoparticles. After subcutaneous implantation, blood was drawn from mice every three days, and CTCs were successfully detected in all implanted subjects. From 100 µl blood samples, the minimum amount found in blood was 9-34 CTCs on 3 day and the maximum was 94-107 CTCs on 15 day. Besides, the isolated CTCs cells remained viable and verified by re-implantation. This study confirms that our multifunctional nanoparticles are highly efficient in detecting CTCs in tumor metastasis and has huge potential in translational medicine.

A Comparison of Fitness-Related Traits in the Coleopteran Parasitoid Dastarcus helophoroides (Coleoptera: Bothrideridae) Reared on Two Factitious Hosts.

The coleopteran parasitoid Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is considered an efficient preventative method against the damage caused by a large number of cerambycid pests in China and other East Asian countries. Mass rearing of this parasitoid depends on screening appropriate factitious hosts. In this paper, the fitness-related traits of this parasitic beetle were explored using pupae of the two tenebrionid beetles, Tenebrio molitor Linnaeus and Zophobas morio Fabricius (Coleoptera: Tenebrionidae) as factitious hosts. The results showed that in dual-choice trials, D. helophoroides larvae preferred to attack Z. morio pupae. In no-choice trials, D. helophoroides reared on Z. morio pupae displayed significantly higher pupation and emergence rate, longer developmental duration of larval and larval-pupal stages, larger adult body size, shorter preoviposition period, more egg masses and egg production, and higher relative expected reproduction than those reared on T. molitor pupae. These results demonstrate that Z. morio is a more suitable factitious host for mass rearing of D. helophoroides.

A new Atrococcus species (Hemiptera, Coccomorpha, Pseudococcidae) from China, with a key to Chinese species.

A new mealybug species Atrococcus rushuiensis Zhang, sp. nov., collected under the leaf sheath of Sporobolus fertilis (Poaceae) in Fuzhou City, Jiangxi Province, China, is described and illustrated. A new combination is introduced, transferring Allotrionymus shanxiensis Wu to the genus Atrococcus as A. shanxiensis (Wu), comb. nov. A key is presented for the species of Atrococcus recorded from China.

Larval Host Preference and Suitability for the Sawfly Mesoneura rufonota among Five Cinnamomun Tree Species.

The camphor sawfly, Mesoneura rufonota Rohwer, is an economically important leaf-chewing pest of the genus Cinnamomum Trew. However, little is known about the range of species that it can infest within this genus or whether larvae show preferences for particular species. We conducted preference and performance experiments under laboratory conditions to assess larval host preference and suitability as a host plant of five congeneric trees species: C. camphora (Linn) Presl, C. bodinieri Levl., C. burmanni (Nees et T. Nees) Blume, C. pauciflorum Nees, and C. micranthum (Hay.) Hay. In no-choice, two-choice and multiple-choice feeding trials, significantly higher feeding rates were found on C. camphora, followed by C. bodinieri, compared to those on the other three tree species. In two-choice behavior trials, larvae moved to occupy leaves of C. camphora faster and more frequently, followed by C. bodinieri, than when offered the other three tree species. In no-choice fitness trials, the survival of larval and pupal stage was the highest, the developmental duration of larval and pupal stage was the shortest, the pupal body weight was the heaviest and adults lived the longest on C. camphora followed by C. bodinieri, while M. rufonota did not complete development on C. burmanni, C. pauciflorum or C. micranthum. Our results demonstrate that larvae consistently prefer and perform better on C. camphora leaves, that they can utilize C. bodinieri, but it is less preferred, and that C. burmanni, C. pauciflorum, and C. micranthum appear to be unsuitable as a host for M. rufonota.

Phytochemicals of garlic: Promising candidates for cancer therapy.

Of the numerous health benefits of garlic, the anticancer effect is probably the most noticeable. Observations over the past years have shown that the consumption of garlic in the diet provides strong protection against cancer risk. Previous studies involving garlic phytochemicals have usually focused on the cancer chemopreventive properties, but there is little published evidence showing its therapeutic potential in cancer treatment. In view of the multitargeted carcinoma actions and lack of severe toxicity, some components of garlic are likely to play vital roles in the selective killing of cancer cells. However, the rational design of experimental studies and clinical trials are required to verify this concept. This paper discusses the promises and pitfalls of garlic for the treatment of cancer.

Cell-free expression, purification and characterization of Drosophila melanogaster odorant receptor OR42a and its co-receptor.

Olfactory receptors (OR), a group of classic membrane proteins, plays a vital role in insect reproduction and acclimatization. Deciphering the molecular mechanism of insect olfaction could enhance pest control and environmental protection. Studies on ORs have faced a major bottleneck due to the notoriously strong hydrophobicity of ORs, which results in difficult expression in heterologous cell systems. Here, we demonstrated that insect ORs could be functionally produced using the E. coli cell-free protein synthesis system (CFPS), in which the highest yield of total ORs is 350 µg per 1 ml reaction. We tested the effects of detergent types and concentrations on soluble expression of ORs. The ORs showed a classic α-helical infrared spectrum. Quartz crystal microbalance (QCM) was used to demonstrate that ORs fold correctly and respond to their ligands. This is the first report that insect OR42a could be functionally produced in vitro. This approach may facilitate the development of biomimetic olfactory biosensors and may also be utilized for drug positioning and development, environmental protection and agriculture.

Life history traits in a capital breeding pine caterpillar: effect of host species and needle age.

BACKGROUND: For capital breeding Lepidoptera, larval food quality is a key determinant of their fitness. A series of studies have suggested that the larval host species or varieties dramatically impact their development and reproductive output. However, few studies have reported the role of foliar age and adult mating success has often been ignored in these studies. In this paper, the influence of host species and needle age on larval performances, adult mating behavior and fitness consequences has been studied using a capital breeding caterpillar, Dendrolimus punctatus Walker (Lepidoptera:Lasiocampidae). RESULTS: In larval performance trial, a strong effect of larval host species and needle age was found on survivorship, developmental duration, body weight, percentage of adult emergence, and growth index, but not on percentage of female progeny. In adult mating trial, larval host species and needle age also significantly affected mating latency and mating duration, but not mating success. In adult fitness trial, female fecundity, longevity and fitness index, but not oviposition duration and fertility, influenced by larval host species and needle age. CONCLUSIONS: These results reveal the importance of larval host species and needle age on larval performance and adult reproductive fitness in this capital breeding insect and provide strong evidence that old needles of masson pine P. massoniana is the best host for D. punctatus.