Correction: Investigation of inerter-based suspension systems for heavy vehicles.

[This corrects the article DOI: 10.1371/journal.pone.0280290.].

A New Gene SCY3 Homologous to Scygonadin Showing Antibacterial Activity and a Potential Role in the Sperm Acrosome Reaction of Scylla paramamosain.

In the study, a new gene homologous to the known antimicrobial peptide Scygonadin was identified in mud crab Scylla paramamosain and named SCY3. The full-length sequences of cDNA and genomic DNA were determined. Similar to Scygonadin, SCY3 was dominantly expressed in the ejaculatory ducts of male crab and the spermatheca of post-mating females at mating. The mRNA expression was significantly up-regulated after stimulation by Vibrio alginolyticus, but not by Staphylococcus aureus. The recombinant protein rSCY3 had a killing effect on Micrococcus luteus and could improve the survival rate of mud crabs infected with V. alginolyticus. Further analysis showed that rSCY3 interacted with rSCY1 or rSCY2 using Surface Plasmon Resonance (SPR, a technology for detecting interactions between biomolecules using biosensor chips) and Mammalian Two-Hybrid (M2H, a way of detecting interactions between proteins in vivo). Moreover, the rSCY3 could significantly improve the sperm acrosome reaction (AR) of S. paramamosain and the results demonstrated that the binding of rSCY3, rSCY4, and rSCY5 to progesterone was a potential factor affecting the sperm AR by SCYs on. This study lays the foundation for further investigation on the molecular mechanism of SCYs involved in both immunity and physiological effects of S. paramamosain.

Investigation of inerter-based suspension systems for heavy vehicles.

The inerter is a two-terminal component that can be added to the spring-and-damper configuration of a suspension system. It has the property that the force exerted is proportional to the relative acceleration at its terminals. Studies have demonstrated the inerter's benefit of providing superior vibration isolation when it is used in the vehicle suspension of passenger cars. However, similar benefit on another common vehicle class on the roads, namely heavy vehicles, remain to be shown, as these vehicles have vastly different parameter values than passenger cars. This study is an investigation on the performance improvement brought by an inerter in the suspension of common heavy vehicles. In the study, the parameter values of a truck and a bus were adopted in the quarter vehicle model with two different spring-damper-inerter configurations (parallel and serial inerter), and the improvements in vibration isolation and road holding capability were determined by optimization of inertance. Results show that the inerter is similarly effective in providing the said improvements when implemented on heavy vehicles instead of on passenger cars, judging from reductions in sprung mass acceleration and dynamic tire load. It is also observed that the performance benefit is associated with larger optimum inertance than that for passenger cars. Overall, the inerter has been shown to be beneficial in the parallel and serial configurations, both of which are common and can be practically implemented in the suspension of heavy vehicles.

Univariate and Multivariate QTL Analyses Reveal Covariance Among Mineral Elements in the Rice Ionome.

Rice provides more than one fifth of daily calories for half of the world's human population, and is a major dietary source of both essential mineral nutrients and toxic elements. Rice grains are generally poor in some essential nutrients but may contain unsafe levels of some toxic elements under certain conditions. Identification of quantitative trait loci (QTLs) controlling the concentrations of mineral nutrients and toxic trace metals (the ionome) in rice will facilitate development of nutritionally improved rice varieties. However, QTL analyses have traditionally considered each element separately without considering their interrelatedness. In this study, we performed principal component analysis (PCA) and multivariate QTL analyses to identify the genetic loci controlling the covariance among mineral elements in the rice ionome. We resequenced the whole genomes of a rice recombinant inbred line (RIL) population, and performed univariate and multivariate QTL analyses for the concentrations of 16 elements in grains, shoots and roots of the RIL population grown in different conditions. We identified a total of 167 unique elemental QTLs based on analyses of individual elemental concentrations as separate traits, 53 QTLs controlling covariance among elemental concentrations within a single environment/tissue (PC-QTLs), and 152 QTLs which determined covariation among elements across environments/tissues (aPC-QTLs). The candidate genes underlying the QTL clusters with elemental QTLs, PC-QTLs and aPC-QTLs co-localized were identified, including OsHMA4 and OsNRAMP5. The identification of both elemental QTLs and PC QTLs will facilitate the cloning of underlying causal genes and the dissection of the complex regulation of the ionome in rice.

Analysis of pathogenetic process of fungal rhinosinusitis: Report of two cases.

BACKGROUND: Fungal rhinosinusitis is an infectious and/or allergic disease caused by fungi in the sinus and nasal cavity. Due to the warm and humid climate in Guangxi Zhuang Autonomous Region, the incidence of fungal rhinosinusitis is higher than that in other provinces. However, its physiological mechanism is not yet clear. Not every patient colonized by fungi develops a fungal infection. To a large extent, the immune status of the patient determines the nature of fungal disease in the nasal passages. The pathologic process of progression from harmless fungal colonization to fungal rhinosinusitis is unclear and has not been reported. CASE SUMMURY: We report two patients, one who developed fungal rhinosinusitis 1.5 years after surgery performed to treat an inverted papilloma, and the other with a history of hypertension and cerebral infarction. Both patients recovered from their surgeries. An average time of 2.5 years elapsed from the development of maxillary sinus cysts to the development of fungal rhinosinusitis. CONCLUSION: According to these case reports, we speculate that the progression of fungal rhinosinusitis from harmless colonization to disease onset requires approximately one to three years and that the length of the process may be related to underlying diseases, surgical treatment, deficient autoimmune status, and abuse of hormone antibiotics and hormones. Additional data are needed to conduct relevant studies to appropriately prevent and treat fungal rhinosinusitis.

Clinical characterisation and cytological study of dry eye in patients with autoimmune disease.

OBJECTIVE: To assess the clinical characteristics and changes in ocular surface cytology of dry eye in patients with systemic autoimmune disease. METHODS: The case-control study was conducted in the Second Hospital of Tianjin Medical University, Tianjin, China, from February 2016 to January 2017, and comprised systemic autoimmune disease patients and healthy controls. Schirmer's I test, tear breakup time test, and fluorescein staining were performed on all subjects. Both groups were evaluated for dry eye with the current diagnostic criteria. Conjunctival impression cytology and the morphology of epithelial cells were observed in both groups of subjects. Flow cytometry was used to identify the amount of apoptosis. SPSS 15 was used to analyse the data. RESULTS: Each of the two groups had 60(50%) subjects each. The morbidity of dry eye in the control group was 17(28.3%), while it was 31(51.7%) in the patients (p<0.01). Among the patients with dry eye, the severity level of cells obtained by conjunctival impression sampling was significantly higher in patients than in controls (p<0.01). The percentage of conjunctival epithelial cells undergoing apoptosis was higher in patients with dry eye than in patients without dry eye in each group, and among patients with dry eye, the percentage of conjunctival epithelial cells undergoing apoptosis was higher in the patients than in controls (p<0.01 each). CONCLUSIONS: The cell injury on the ocular surface was more serious in subjects with dry eye in systemic autoimmune disease than in subjects with dry eye in healthy controls.

[A Cross-sectional Study of Hepatitis B Virus Infection in Mianyang, Sichuan Province].

OBJECTIVES: To determine the prevalence and distribution of hepatitis B virus (HBV) infection in Mianyang. METHODS: Data were extracted from the 12th five-year National Science and Technology Major Projects-Integrated Prevention and Control of Major Infectious Diseases in Mianyang. A two-level logistic regression model was established to determine factors associated with HBV infection. RESULTS: About 4.91% of people in Mianyang were HBsAg positive, which increased with age. HBV infection showed aggregation at townships. Governmental spending ≥¥1 000 000 on public health was a protective factor in the regression model; whereas, age, male gender, medical workers, absent from HBV vaccination, more than 80 g/d alcohol consumption were risk factors of HBV infection. CONCLUSIONS: Mianyang had medium level of HBV infections. But high HBV prevalence can be found in some townships. The known behavior risk factors all exist in Mianyang, which can serve as a screening tool for identifying high risk populations.

Primary bone lymphoplasmacytic lymphoma presenting with spinal cord compression: a case report.

UNLABELLED: Primary bone lymphoma is a rare disease, and the main pathological type is diffuse large B-cell lymphoma. The occurrence of follicular, marginal zone and lymphoplasmacytic lymphomas is rare. Vertebras are also sites that can be affected, and spinal cord compression is reported in 14% of patients with vertebral involvement. However, there is no report on primary vertebral lymphoplasmacytic lymphoma with spinal cord compression. The present report presents one case of primary vertebral lymphoplasmacytic lymphoma with spinal cord compression and increased serum and urine λ light chain, without an elevated heavy chain of immunoglobulin. CONFLICT OF INTEREST: None declared.

Shikonin derivatives protect immune organs from damage and promote immune responses in vivo in tumour-bearing mice.

Shikonin, a major component of Lithospermum erythrorhizon and Arnebia euchroma, exhibits antiinflammatory, immunomodulatory and antitumour activities. Although many recent studies have focused on the antitumour effects of shikonin, the exact mechanisms underlying its antitumour and immunomodulatory effects in tumour-bearing mice remain unclear. The aim of the present study was to investigate the antitumour and immunomodulatory effects of shikonin derivatives (ShD) in tumour-bearing mice. Swiss mice inoculated with hepatoma HepA(22) or sarcoma 180 (S(180)) cells were treated with ShD or 5-fluorouracil (5Fu). Survival time, immune organs, natural killer cell activity, lymphocytes, lymphocyte transformation and interleukin (IL)-2 production were analysed. ShD significantly prolonged the survival (median survival time prolonged by >7 days) of tumour-bearing mice in a dose-dependent manner, inhibited the growth of transplantable neoplasms (inhibitory rate, > 33%), and recovered (at [ShD] = 2.5 mg/kg/day) or increased (at [ShD] > 5 mg/kg/day) the number of CD3- and CD19-positive cells. ShD also played a role in protecting the immune organs from damage and reversed or enhanced immune responses, as noted by the nearly normal thymic structure; enlarged splenic corpuscles; and improved natural killer cell activity, lymphocyte transformation and IL-2 production in ShD-treated mice. ShD reduced the tumour load of tumour-bearing mice and protected the immune organs against tumour-induced damage and immune function impairment.

Days in milk at first AI in dairy cows; its effect on subsequent reproductive performance and some factors influencing it.

The aim of the present study was to show the distribution of cows by days in milk (DIM) at first AI, effect of DIM at first AI on reproductive performance and some factors influencing DIM at first AI. A total of 767 Holstein Frisian cows that calved from January 2004 to December 2007 in 14 dairy herds were used. The first AI conception rate (FAICR) was 34.0%. Seventy-five percent of the cows were first inseminated within 100 days after calving. FAICR increased linearly up to 100 DIM. A one unit (20 days) longer DIM at first AI within the first 100 days postpartum increased the likelihood of a 2.4% FAICR. However, cows first inseminated at an earlier stage of lactation showed better reproductive performance in terms of pregnancy rate and calving to conception interval than cows first inseminated at a later stage. A one day increase in the interval from calving to first AI reduced the likelihood of 0.85 days to become pregnant. Herd or region located in southwestern Japan, tie-stall herd, first AI in spring, higher parity, uterine infection, metabolic diseases and/or mastitis and abnormal resumption of postpartum ovarian cycles contributed to delaying first AI.

Analysis of some factors affecting fertility levels in a high-producing dairy herd in south-western Japan.

The present study aimed to know whether all cows have been showing declining fertility or only a proportion of cows are attributed to the declining fertility, and to describe factors affecting the level of fertility. A total of 131 cows calved from February 2005 to December 2007 in a dairy herd were examined. Fourteen cows were excluded from the study because of early culling. Of the remaining 117 cows, 47 (40%) conceived within 115 days postpartum after 1-3 artificial insemination (AI) (normal fertility cows), 42 (36%) conceived after 115 days postpartum following 1-3 AI or were culled after 1-2 (sub-fertility cows/culled), and 28 (24%) were inseminated more than three times without detectable genital tract abnormalities (repeat breeders). Calving to conception interval in the normal fertility group was 72 + 3 days, while in the sub-fertility/culled and repeat breeding groups the intervals were 170 + 8 and 259 + 16 days, respectively. Endometritis was the risk factor for sub-fertility/culled (odds ratio (OR) = 3.76). Prolonged luteal phase (OR = 4.08), delayed first ovulation (OR = 6.02), and delayed corpus luteum formation after AI (OR = 8.55) were the risk factors for repeat breeding. In conclusion, 60% cows showed reduced fertility in a herd, while the other 40% had normal fertility. Uterine infection and some ovarian disorders contributed to reduced fertility.

Reproductive performance of repeat breeders in dairy herds.

The objectives were to characterize repeat breeding in dairy cows, including reproductive performance and risk factors. Data from 613 Holstein Friesian cows in nine dairy herds across Japan were enrolled. A repeat breeder was defined as a cow that did not become pregnant after three inseminations, despite no clinically detectable reproductive disorders. In contrast, cows that became pregnant within three inseminations were considered to have normal fertility. Of the 613 cows, 87.3% eventually became pregnant after repeated AI (maximum calving to conception interval was 435 d). Mean (+/-SEM) first AI conception rate, days in milk at first AI, calving to conception interval and service per conception were 38.3%, 82+/-2 d, 125+/-3 d, and 2.0+/-0.1 times, respectively. Normal fertility cows (n=479) required only 114+/-3 d to conceive and 1.7+/-0.1 inseminations per pregnancy, whereas repeat breeders (n=86) required significantly more days to conceive (211+/-10) and more inseminations per pregnancy (4.7+/-0.2). Based on survival analysis, it took 94 d after calving for 50% of normal fertility cows to become pregnant, compared to 155 d for repeat breeders. For repeat breeders, 31.4, 50.0, and 58.1% became pregnant within 210, 300, and 435 d after calving, respectively. The risk factors for repeat breeding were parity (relative risk [RR]=0.809; P=0.058), resumption of postpartum ovarian cycles (RR=1.928; P=0.009), and days in milk at first AI (RR=0.991; P=0.039). In conclusion, repeat breeder dairy cows had very poor reproductive performance. Lower parity, abnormal resumption of postpartum ovarian cycles, and shorter days in milk at first AI were risk factors for repeat breeding.

Effect of CIDR 12 to 19 days after AI on detection of returning estrus and conception rate in dairy cows.

Detection of returning estrus in dairy cows after AI and re-insemination without delay are important in shortening the calving to conception interval. The objectives of this study were to show the effectiveness of CIDR insertion 12 to 19 days after AI on returning estrus and shortening the calving to conception interval in dairy cows. Seventy-nine dairy cows from two commercial dairy farms were synchronized for first postpartum estrus using a CIDR-Heatsynch protocol, and 76 cows (96.2%) showed estrus signs within 2 days after EB injection and were inseminated. The cows were then divided randomly into two groups. Thirty-seven cows were treated with a CIDR from 12 to 19 days after AI (CIDR group), while the other 39 cows were not treated and served as a control group. Milk samples were collected twice weekly from one week before the commencement of the CIDR-Heatsynch protocol until 7 to 9 days after removal of device. Detection rates of returning estrus 20 to 25 days after AI (within 6 days after removal of the device) were 30.4% in the CIDR group and 47.6% in the control group. According to the progesterone profiles, almost half of the non-pregnant cows that did not show estrus 20 to 25 days after AI had high progesterone concentrations from days 20 to 25, 59.1% in the CIDR group and 50.0% in control group. The calving interval was not significantly different between the CIDR (162 +/- 50 days) and control groups (151 +/- 40 days). In conclusion, CIDR insertion 12 to 19 days after AI did not increase the detection rate of returning estrus. As a consequence, there was no effect of the CIDR treatment on the calving to conception interval.

[Effect of hepatitis B virus C protein on function of natural killer cell in NK-92 cells].

OBJECTIVE: To investigate the influence of hepatitis B virus C protein on the function of natural killer cell. METHODS: Recombinant eukaryotic expression plasmid pHBI-CMV-HBC was constructed and confirmed by double restrictive enzyme digestion and DNA sequencing analysis. Then the recombinant plasmid was transfected into NK-92 cells with lipofectamine encapsuled. The transfected NK-92 cells containing expressive HBV C protein was confirmed by Western Blot analysis. ELISA was employed to determine the IFN-gamma level secreted by NK-92 cells. And finally the cytotoxicities of NK cells were analysed by MTT colorimetry, with the hepatoblastoma cell line (HepG2) as target cell. RESULTS: Western blotting confirmed the expression of HBV C protein in the NK-92 cells transfected with pHBI-CMV-HBC. NK cytotoxicities and IFN-gamma secretion level of NK-92 cells transfected with recombinant plasmid significantly increased compared to control NK-92 cells transfected with blank plasmid (P < 0.01) and untransfected NK-92 cells(P < 0.01). CONCLUSION: Transient expression of HBC can increase IFN-gamma secretion and cytotoxicities of NK-92 cells.

Self-directed self-assembly of nanoparticle/copolymer mixtures.

The organization of inorganic nanostructures within self-assembled organic or biological templates is receiving the attention of scientists interested in developing functional hybrid materials. Previous efforts have concentrated on using such scaffolds to spatially arrange nanoscopic elements as a strategy for tailoring the electrical, magnetic or photonic properties of the material. Recent theoretical arguments have suggested that synergistic interactions between self-organizing particles and a self-assembling matrix material can lead to hierarchically ordered structures. Here we show that mixtures of diblock copolymers and either cadmium selenide- or ferritin-based nanoparticles exhibit cooperative, coupled self-assembly on the nanoscale. In thin films, the copolymers assemble into cylindrical domains, which dictate the spatial distribution of the nanoparticles; segregation of the particles to the interfaces mediates interfacial interactions and orients the copolymer domains normal to the surface, even when one of the blocks is strongly attracted to the substrate. Organization of both the polymeric and particulate entities is thus achieved without the use of external fields, opening a simple and general route for fabrication of nanostructured materials with hierarchical order.

A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes.

Copper(I)-catalyzed 1,3-dipolar cycloaddition reaction of nonfluorescent 3-azidocoumarins and terminal alkynes afforded intense fluorescent 1,2,3-triazole products. The mild condition of this reaction allowed us to construct a large library of pure fluorescent coumarin dyes. Since both azide and alkyne are quite inert to biological systems, this reaction has potential in bioconjugation and bioimaging applications. [reaction: see text]