Low loss, wideband, and high extinction ratio TM polarizer based on subwavelength gratings.

We propose a low loss, wideband silicon transverse magnetic (TM) polarizer with high polarization extinction ratio and low reflection based on subwavelength grating. By arranging and optimizing a mutually perpendicular subwavelength grating with different duty cycles as the core and cladding, efficient waveguiding and radiation can be achieved for the TM and transverse electric (TE) injection, respectively. In simulation, the proposed TM polarizer has a footprint of 40µm×16.68µm, an insertion loss <0.7d B, a polarization extinction ratio ≥20d B, and an unwanted TE reflection <-17.4d B in the wavelength range of 1230-1700 nm. Moreover, the fabrication tolerance of the proposed device is also investigated.

Compact plasmon modulator based on the spatial control of carrier density in indium tin oxide.

To keep pace with the demands of semiconductor integration technology, a semiconductor device should offer a small footprint. Here, we demonstrate a compact electro-optic modulator by controlling the spatial distribution of carrier density in indium tin oxide (ITO). The proposed structure is mainly composed of a symmetrical metal electrode layer, calcium fluoride dielectric layer, and an ITO propagating layer. The carrier density on the surface of the ITO exhibits a periodical distribution when the voltage is applied on the electrode, which greatly enhances the interaction between the surface plasmon polaritons (SPPs) and the ITO. This structure can not only effectively improve the modulation depth of the modulator, but also can further reduce the device size. The numerical results indicate that when the length, width, and height of the device are 14 µm, 5 µm, and 8 µm, respectively, the modulation depth can reach 37.1 dB at a wavelength of 3.66 µm. The structure can realize a broadband modulation in theory only if we select a different period of the electrode corresponding to the propagating wavelength of SPPs because the modulator is based on the scattering effect principle. This structure could potentially have high applicability for optoelectronic integration, optical communications, and optical sensors in the future.

Tunable bandpass microwave photonic filter with largely reconfigurable bandwidth and steep shape factor based on cascaded silicon nitride micro-ring resonators.

Bandpass microwave photonic filter (MPF) can be achieved based on the well-known phase to intensity conversion method by using phase modulation and single micro-ring resonator (MRR) notch filter. Since MRR could introduce residual phase in handling one optical sideband, the out-of-band radio frequency (RF) rejection ratio and the shape factor of the bandpass MPF are very limited. Here, by introducing another MRR to handle the other optical sideband, the residual phase can be greatly suppressed, thus the filter's performance can be greatly improved. The proposed bandpass MPF was both verified theoretically and experimentally. Compared with the single MRR, the out-of-band RF rejection ratio and the shape factor were improved by 20 dB and 1.67, respectively. Furthermore, the bandpass MPF's bandwidth is reconfigurable by adjusting the optical carrier's frequency or the two MRRs' amplitude coupling coefficients. The bandpass MPF's center frequency is also tunable by changing the resonant wavelengths of two MRRs in the opposite direction simultaneously. Experimentally, bandwidth reconfiguration from 0.38 GHz to 15.74 GHz, the shape factor optimization from 2 to 1.23, and frequency tuning from 4 GHz to 21.5 GHz were achieved. We believe that the proposed bandpass MPF has great potential for microwave photonic signal processing.

Broadly Effective ACE2 Decoy Proteins Protect Mice from Lethal SARS-CoV-2 Infection.

As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have been causing increasingly serious drug resistance problem, development of broadly effective and hard-to-escape anti-SARS-CoV-2 agents is an urgent need. Here, we describe further development and characterization of two SARS-CoV-2 receptor decoy proteins, ACE2-Ig-95 and ACE2-Ig-105/106. We found that both proteins had potent and robust in vitro neutralization activities against diverse SARS-CoV-2 variants, including BQ.1 and XBB.1, that are resistant to most clinically used monoclonal antibodies. In a stringent lethal SARS-CoV-2 infection mouse model, both proteins lowered the lung viral load by up to ~1,000-fold, prevented the emergence of clinical signs in >75% animals, and increased the animal survival rate from 0% (untreated) to >87.5% (treated). These results demonstrate that both proteins are good drug candidates for protecting animals from severe COVID-19. In a head-to-head comparison of these two proteins with five previously described ACE2-Ig constructs, we found that two constructs, each carrying five surface mutations in the ACE2 region, had partial loss of neutralization potency against three SARS-CoV-2 variants. These data suggest that extensively mutating ACE2 residues near the receptor binding domain (RBD)-binding interface should be avoided or performed with extra caution. Furthermore, we found that both ACE2-Ig-95 and ACE2-Ig-105/106 could be produced to the level of grams per liter, demonstrating the developability of them as biologic drug candidates. Stress condition stability testing of them further suggests that more studies are required in the future to improve the stability of these proteins. These studies provide useful insight into critical factors for engineering and preclinical development of ACE2 decoys as broadly effective therapeutics against diverse ACE2-utilizing coronaviruses. IMPORTANCE Engineering soluble ACE2 proteins that function as a receptor decoy to block SARS-CoV-2 infection is a very attractive approach to creating broadly effective and hard-to-escape anti-SARS-CoV-2 agents. This article describes development of two antibody-like soluble ACE2 proteins that broadly block diverse SARS-CoV-2 variants, including Omicron. In a stringent COVID-19 mouse model, both proteins successfully protected >87.5% animals from lethal SARS-CoV-2 infection. In addition, a head-to-head comparison of the two constructs developed in this study with five previously described ACE2 decoy constructs was performed here. Two previously described constructs with relatively more ACE2 surface mutations were found with less robust neutralization activities against diverse SARS-CoV-2 variants. Furthermore, the developability of the two proteins as biologic drug candidates was also assessed here. This study provides two broad anti-SARS-CoV-2 drug candidates and useful insight into critical factors for engineering and preclinical development of ACE2 decoys as broadly effective therapeutics against diverse ACE2-utilizing coronaviruses.

A multi-parameter tunable plasmon modulator.

Multi-parameter control of light is a key functionality to modulate optical signals in photonic integrated circuits for various applications. However, the traditional optical modulators can only control one or two properties of light at the same time. Herein, we propose a hybrid structure which can modulate the amplitude, wavelength and phase of surface plasmon polaritons (SPPs) simultaneously to overcome these limitations. The numerical results show that when the Fermi level of graphene changes from 0.3 to 0.9 eV, the variation of optical transmission, wavelength and phase are 32.7 dB, 428 nm and 306°, respectively. The demonstrated structure triggers an approach for the realization of ultracompact modulation and has potential applications in the fields of optical switches, communications and photo-detection.

Frequency-selectable microwave generation based on on-chip switchable spectral shaping and wavelength-to-time mapping.

We propose and experimentally demonstrate a scheme for the photonic generation of pulsed microwave signals with selectable frequency based on spectral shaping and wavelength-to-time mapping (WTTM) technique. The frequency selectivity is realized by channel switching on an integrated silicon-on-insulator (SOI) spectral shaping chip. The incident signal is spectrally shaped by the asymmetric Mach-Zehnder interferometer (MZI) in the selected channel, and an optical spectrum with uniform free spectral range (FSR) can be generated in a broad bandwidth up to dozens of nanometers, implying large microwave signal duration after WTTM if a pulse light source with matched bandwidth is available. Microwave pulses of frequency from 3.6 GHz to 28.4 GHz with a fixed interval are experimentally generated respectively. The realization of eight microwave frequencies selectable with only one shared dispersive element (DE) required indicates high expansibility in the frequency cover range of our scheme by tuning the dispersion value in WTTM.

Achieving Fano resonance with an ultra-high slope rate by silicon nitride CROW embedded in a Mach-Zehnder interferometer.

Fano resonance with asymmetric line shape is very promising in many applications such as optical switching, sensing, slow light, laser. Fano resonances based on some integrated structures have been demonstrated on the silicon on insulator platform. However, the extinction ratios and slope rates of the most proposed integrated Fano resonances are relatively low, which limits their applications. In this paper, a tunable silicon nitride coupled resonator optical waveguide (CROW) embedded in a Mach-Zehnder interferometer (MZI) is proposed to achieve Fano resonance. Benefiting from fine tuning supported by the low thermo-optic coefficient of the silicon nitride optical waveguide, the optical amplitudes and phases in the two arms of the MZI were accurately adjusted to achieve destructive interference, which gives an ultra-high extinction ratio. Furthermore, high quality factor CROW, supported by the native low loss silicon nitride optical waveguide, greatly shrinks the resonance bandwidth. Combining the above two superiorities, a Fano resonance with a very high extinction ratio of up to 57 dB and slope rate as high as 8.1 × 104 dB/nm was obtained, which is an order of magnitude larger than the reported integrated Fano resonances. We believe that the proposed structure would be a promising candidate for high-performance switching and high-sensitivity sensing.

Compact and broadband silicon mode-order converter using bricked subwavelength gratings.

A compact and broadband silicon mode-order converter (MOC) scheme by employing reciprocal mode evolution between asymmetric input/output taper and bricked subwavelength gratings (BSWG) is proposed. In the proposed MOC, a quasi-TE0 mode is generated in the BSWG region, which can be regarded as an effective bridge between the two TE modes to be converted. Flexible mode conversion can be realized by only choosing appropriate structure parameters for specific mode transitions between input/output modes and the quasi-TE0 mode. By combing 3D finite difference time domain (FDTD) and particle swarm optimization (PSO) method, TE0-TE1 and TE0-TE2 MOCs are optimal designed, which can efficiently convert TE0 mode to TE1 and TE2 modes with lengths of 9.39 µm and 11.27 µm, respectively. Results show that the insertion losses of <1 dB and crosstalk of <-15 dB are achieved for both TE0-TE1 and TE0-TE2 MOCs, the corresponding working bandwidth are 128 nm (1511∼1639 nm) and 126 nm (1527∼1653 nm), respectively. Additionally, the MOCs can be fabricated with only single etch step with minimum feature size of 145 nm.

Tunable microwave frequency comb generation using a Si3N4-MDR based actively mode-locked OEO.

Microwave frequency combs (MFCs) have important applications in communication and sensing owing to their characteristics of large number of comb lines, wide frequency range, and high precision of comb spacing. In many applications, MFCs are required to emit signals with tunable center frequency and variable comb spacing to accommodate different operating frequency bands and accuracies. Here, we demonstrate a tunable MFC by injecting a low-frequency electrical signal into a tunable optoelectronic oscillator (OEO). Tuning of MFC's center frequency and comb spacing are realized, allowing a frequency tuning range from 1 to 22 GHz and 50 comb lines within a 5 MHz bandwidth obtained in the MFC generator. In addition, the introduction of the silicon nitride micro-disk resonator (Si3N4-MDR) in the system paves the way for the integration of MFC generator.

Low loss silicon nitride 1×4 microwave photonic beamforming chip.

In this paper, based on the low loss double strip silicon nitride platform, we designed and fabricated an ultra-low loss 1×4 microwave photonic beamforming chip, which contains a 1×4 beam splitter and four 5-bit optical delay lines. Each optical delay line can achieve 32 delay states varying from 0 ps to about 130 ps, which can support 21 different beamforming angles covers from -56.42° to 56.68° for 10 GHz RF signal. A low on-chip insertion loss of about 4 dB is achieved for each 5-bit optical delay line. Furthermore, a very low loss delay ratio of about 0.0016 dB/ps is achieved and a recorded low loss fluctuation of about 0.3 dB is obtained during the 32 states delay switching. In addition, the switching speed and driving power consumptions of the proposed beamforming chip were investigated. The proposed beamforming chip could have great potential in optical controlled phased antenna arrays systems.

Compact plasmon modulator with a high extinction ratio.

To keep pace with the demands in optical communications, electro-optic modulators should feature a high extinction ratio, offer a small footprint, and allow for practical detection. Herein, we demonstrate a compact plasmon modulator with a high extinction ratio where a compact modulation region composed of indium tin oxide (ITO) is embedded to the arms of the Mach-Zehnder (M-Z) interferometer. The modulator has a footprint of 20µm×12µm with a modulation region of 4µm×0.5µm. The numerical results show that the extinction ratio is 15.2 dB when the electron concentration of ITO is changed 4×1020cm-3. This type of modulator paves the way for future compact optoelectronic integration and has potential application in the fields of optical communication, photodetection, and sensing.

Electrical Switching of the Off-Resonance Room-Temperature Valley Polarization in Monolayer MoS2 by a Double-Resonance Chiral Microstructure.

Enhancing and expanding the manipulated range of room-temperature valley polarization at off-resonance wavelength is extremely crucial to developing various functional valleytronic devices. Although these have been realized through the double-resonance strategy or twist-angle engineering, the demand for electrical control over the concepts remains elusive. Here, we fabricate a gate-tunable double-resonance chiral microstructure using a molybdenum disulfides (MoS2) monolayer. On the basis of the varied interface charge density, we demonstrate the huge photoluminescence (PL) tuning ability of this configuration. Furthermore, benefiting predominately from the screening of long-range e-h exchange interactions and the chiral Purcell effect, the electrical switching of the room-temperature valley polarization at off-resonance wavelength is also realized. Our work enriches the functions of TMDs-based optoelectronic devices and may create important applications in future valley-polarized encode and information processing devices.

Photonic generation of broadband linearly chirped microwave waveform based on a low-loss silicon on-chip spectral shaper.

A silicon on-chip spectral shaper based on a Sagnac loop incorporating a chirped multi-mode waveguide Bragg grating (WBG) for linearly chirped microwave waveform generation is fabricated and demonstrated. The transmission spectrum of the spectral shaper displays low insertion loss characteristic due to the application of edge coupling taper and multi-mode waveguide based grating. An up-chirped microwave waveform with bandwidth as large as 44 GHz is generated by mapping the spectrum profile of the spectral shaper to the temporal domain through a dispersion fiber. The instantaneous frequency of the generated signal shows good linearity benefiting from the weak modulation strength in the multi-mode WBG. The low insertion loss performance as well as the low dispersion value required in our design presents feasibility in further integration with on-chip dispersion.

Parity-time symmetric tunable OEO based on dual-wavelength and cascaded PS-FBGs in a single-loop.

The ability to achieve low phase noise single-mode oscillation within an optoelectronic oscillator (OEO) is of fundamental importance. In the frequency-tunable OEO, the wide microwave photonic filter (MPF) bandwidth is detrimental to select single-mode among the large number of cavity modes, thus leading to low signal quality and spectral purity. Stable single-mode oscillation can be achieved in a large time delay OEO system by harnessing the mechanism from parity-time (PT) symmetry. Here, a PT-symmetric tunable OEO based on dual-wavelength and cascaded phase-shifted fiber gratings (PS-FBGs) in a single-loop is proposed and experimentally demonstrated. Combining the merits of wide frequency tuning of PS-FBG-based MPF and single mode selection completed by the PT-symmetric architecture of the OEO, where the gain and loss modes carried by dual-wavelengths to form two mutually coupled resonators in a single-loop, signals range from 1 GHz to 22 GHz with the low phase noise distributed in -122∼ -130 dBc/Hz at 10 kHz offset frequency are obtained in the experiment.

Broadband and compact polarization beam splitter in LNOI hetero-anisotropic metamaterials.

In this paper, theoretical modeling and numerical simulations of a high-performance polarization beam splitter (PBS) based on hetero-anisotropic metamaterials are proposed on the lithium-niobate-on-insulator (LNOI) platform. The hetero-anisotropic metamaterials constructed by sub-wavelength gratings (SWGs) can be regarded as effective anisotropy medium, which exhibits strong birefringence without breaking the geometrical symmetry, contributing to the formation of PBS. Rather than the principle of PBS based on beat-length difference of transverse electric (TE) polarization and transverse magnetic (TM) polarization, the device can realize polarization beam splitting in single beat length, and the footprint of the proposed PBS can be reduced to 8 µm × 160 µm (with S-bend). The simulation results show that the bandwidth is 185 nm (1450∼1634 nm) for TE polarization while the bandwidth is 85 nm (1490∼1575 nm) for TM polarization when the polarization extinction ratio is >20 dB. Furthermore, the insertion loss is less than 1 dB in the range of 1450 to 1650 nm, for both TE and TM polarization. Additionally, the proposed device proves strong robustness of the fabrication tolerance.

Tunable asymmetric transmission across stretchable chiral metamaterial.

A stretchable chiral metamaterial with L-shaped and T-shaped Au patterns (SCMM-LT) is proposed to generate asymmetric transmission (AT) for circularly polarized waves on the polydimethylsiloxane substrate in the mid-infrared region. The peak value of AT can reach 50.02% at the resonance wavelength of 19.1 µm, owing to the enantiomerically sensitive plasmons. With stretching along the x axis and the y axis. respectively, the band of AT shifts to a longer wavelength, which proves the SCMM-LT can be a candidate as the tunable chiral metamaterial. In the future, the proposed stretchable chiral metamaterial could potentially possess high applicability for wearable electronic devices in a variety of sensor fields.

The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond-Blackfan anemia.

Diamond-Blackfan anemia (DBA) is a rare, inherited bone marrow failure syndrome, characterized by red blood cell aplasia, developmental abnormalities, and enhanced risk of malignancy. However, the underlying pathogenesis of DBA is yet to be understood. Recently, mutations in the gene encoding ribosomal protein (RP) L18 were identified in DBA patients. RPL18 is a crucial component of the ribosomal large subunit but its role in hematopoiesis remains unknown. To genetically model the ribosomal defect identified in DBA, we generated a rpl18 mutant line in zebrafish, using CRISPR/Cas9 system. Molecular characterization of this mutant line demonstrated that Rpl18 deficiency mirrored the erythroid defects of DBA, namely a lack of mature red blood cells. Rpl18 deficiency caused an increase in p53 activation and JAK2-STAT3 activity. Furthermore, we found inhibitors of JAK2 or STAT3 phosphorylation could rescue anemia in rpl18 mutants. Our research provides a new in vivo model of Rpl18 deficiency and suggests involvement of signal pathway of JAK2-STAT3 in the DBA pathogenesis.

One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish.

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no 'two-in-one' KI strategy combining conditional knockout (CKO) with fluorescent gene-labeling or further allele-labeling has been reported. Here, we developed a dual-cassette-donor strategy and achieved one-step and efficient generation of dual-function KI alleles at tbx5a and kctd10 loci in zebrafish via targeted insertion. These alleles display fluorescent gene-tagging and CKO effects before and after Cre induction, respectively. By introducing a second fluorescent reporter, geno-tagging effects were achieved at tbx5a and sox10 loci, exhibiting CKO coupled with fluorescent reporter switch upon Cre induction, enabling tracing of three distinct genotypes. We found that LiCl purification of gRNA is critical for highly efficient KI, and preselection of founders allows the efficient germline recovery of KI events.

Tunable multifunctional reflection polarizer based on a graphene metasurface.

Herein, we present a tunable multifunctional reflection polarizer, based on a graphene metasurface, which is composed of an array of cross double-ellipse graphene patches. A dual band of linear-to-linear (LTL) polarization conversions is achieved due to the superimposition of the two reflection components with a near 0° or 180° phase difference, in the mid-infrared region. By carefully choosing the parameters, linear-to-circular polarization conversion and broadband of LTL polarization conversion (about 0.7 THz) are also realized. Also, the tunable responses of the proposed reflection polarizer are discussed under a different Fermi energy and electron scattering time. It is believed that our proposed polarizer can be widely used for multifunctional and tunable polarization conversion.

Graphene surface plasmon off-axis superlens based on tilted one-dimensional Si/SiO2 gratings.

Graphene surface plasmon (GSP) superlenses, induced from the negative refraction, have recently been demonstrated in various two-dimensional photonic crystal systems. However, inplane GSP superlenses have never been reported in a one-dimensional (1D) photonic crystal system. Here, we propose a graphene-Si/SiO2 system, by transferring a graphene sheet on the tilted 1D subwavelength silicon/silica gratings. By discussing the dispersion relations of the inplane GSP in this system, the GSP negative refraction is found in the mid-infrared region. When the tilted angle, working wavelength, and Fermi level are set to be 60°, 11.22 µm, and 0.2 eV, respectively, the off-axis subwavelength focusing has the best resolution, and the full width at half-maximum (FWHM) of the image is 0.0091λ (102.1 nm). Further, we investigate the effects of the Fermi level on the superlens frequency range, and the image's FWHM, the broadband, and the deep subwavelength superlens are achieved. The full-wave numerical simulations are conducted by the finite element method. Our findings can be applied to the manipulation of inplane GSP propagation and biological imaging.