Multicopper oxidases GbAO and GbSKS are involved in the Verticillium dahliae resistance in Gossypium barbadense.

Ascorbate oxidase (AO) and skewed5 (SKU5)-similar (SKS) proteins belong to the multicopper oxidase (MCO) family and play important roles in plants in response to environmental stress via modulation of oxidoreduction homeostasis. Currently, reports on the response of Gossypium barbadense MCO to Verticillium wilt (VW) caused by Verticillium dahliae are still limited. Herein, RNA sequencing of two G. barbadense cultivars of VW-resistant XH21 and VW-susceptible XH7 under V. dahliae treatment, combined with physiological and genetic analysis, was performed to analyze the function and mechanism of multicopper oxidases GbAO and GbSKS involved in V. dahliae resistance. The identified differentially expressed genes are mainly involved in the regulation of oxidoreduction reaction, and extracellular components and signaling. Interestingly, ascorbate oxidase family members were discovered as the most significantly upregulated genes after V. dahliae treatment, including GbAO3A/D, GbSKS3A/D, and GbSKS16A/D. H2O2 and Asc contents, especially reductive Asc in both XH21 and XH7, were shown to be increased. Silenced expression of respective GbAO3A/D, GbSKS3A/D, and GbSKS16A/D in virus-induced gene silencing (VIGS) cotton plants significantly decreased the resistance to V. dahliae, coupled with the reduced contents of pectin and lignin. Our results indicate that AO might be involved in cotton VW resistance via the regulation of cell wall components.

Comparative Proteomic Analysis Reveals the Ascorbate Peroxidase-Mediated Plant Resistance to Verticillium dahliae in Gossypium barbadense.

In previous research on the resistance of cotton to Verticillium wilt (VW), Gossypium hirsutum and G. barbadense were usually used as the susceptible and resistant cotton species, despite their different genetic backgrounds. Herein, we present data independent acquisition (DIA)-based comparative proteomic analysis of two G. barbadense cultivars differing in VW tolerance, susceptible XH7 and resistant XH21. A total of 4,118 proteins were identified, and 885 of them were differentially abundant proteins (DAPs). Eight co-expressed modules were identified through weighted gene co-expression network analysis. GO enrichment analysis of the module that significantly correlated with V. dahliae infection time revealed that oxidoreductase and peroxidase were the most significantly enriched GO terms. The last-step rate-limiting enzyme for ascorbate acid (AsA) biosynthesis was further uncovered in the significantly enriched GO terms of the 184 XH21-specific DAPs. Additionally, the expression of ascorbate peroxidase (APX) members showed quick accumulation after inoculation. Compared to XH7, XH21 contained consistently higher AsA contents and rapidly increased levels of APX expression, suggesting their potential importance for the resistance to V. dahliae. Silencing GbAPX1/12 in both XH7 and XH 21 resulted in a dramatic reduction in VW resistance. Our data indicate that APX-mediated oxidoreductive metabolism is important for VW resistance in cotton.

Expression profiling of the mitogen-activated protein kinase gene family reveals their diverse response pattern in two different salt-tolerant Glycyrrhiza species.

BACKGROUND: Mitogen-activated protein kinases (MPKs) play important role in response to environmental stress as crucial signal receptors or sensors. Our previous study indicated that salt stress acts as a positive factor to stimulate the production of pharmacodynamic metabolites in the medicinal plant Glycyrrhiza uralensis. Currently, little is known about the MPK gene family and their functions in the medicinal plant G. uralensis. OBJECTIVE: Identification, comprehensive bioinformatic analysis, expression profiling, and response pattern under salt stress of the G. uralensis GuMPK gene family. METHODS: Genome-wide investigation and expression profiling of the MPK gene family in G. uralensis, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, promoter cis-acting element, and expression pattern under salt stress in two different salt-tolerant Glycyrrhiza species were performed. RESULTS: A total of 20 G. uralensis GuMPK genes were identified and categorized into five groups, and had conserved gene structure and motif distribution. Expression profiling of GuMPK genes suggested their potentially diverse functions in plant growth and in response to phytohormones and environmental stress, particularly GuMPK1, 2, 5, and 10 as key components for G. uralensis in response to abiotic stress. Further expression analysis under NaCl treatment in two different salt-tolerant Glycyrrhiza species displayed the MPKs' different response patterns, emphasizing the role of MPK2, 5, 7, and 16 as potentially crucial genes for Glycyrrhiza to respond to salt stress. CONCLUSION: Our results provide a genome-wide identification and expression profiling of MPK gene family in G. uralensis, and establish the foundation for screening key responsive genes and understanding the potential function and regulatory mechanism of GuMPKs in salt responsiveness.

[N2O Emissions from a Tea Field with Deep Application of Nitrogen Fertilizer and Intercropping with White Clover].

In recent years, the area of tea fields in China has expanded. The application rate of nitrogen fertilizer is usually high in tea fields, which causes high N2O emissions. Tea fields are important sources of N2O emissions; thus, it is necessary to research N2O emission reduction in tea fields. A three-year field study was conducted to investigate soil N2O emissions and influencing factors under different fertilization measurements in a typical tea field in a subtropical hilly region of China. Three treatments-conventional fertilization, deep application of nitrogen fertilizer, and intercropping with clover-were studied to measure the soil N2O fluxes and the related soil and environmental properties. The results showed that the subtropical hilly tea field had high N2O emissions, and the cumulative annual emissions of N2O-N were as high as 5.1-10.1 kg·hm-2. The N2O emissions occurred mainly in spring and summer. When the soil temperature was lower than 15℃, the N2O flux shown mainly a positive correlation with the soil temperature. However, when the soil temperature was higher than 15℃, the positive correlation was mainly with the soil water, soil NH4+-N, and NO3--N contents. Compared with conventional fertilization, the intercropping of white clover did not significantly reduce N2O emissions, although deep application of fertilizer increased annual N2O emissions when the rainfall was high. Neither intercropping of white clover or deep application of fertilizer affected the tea yield or the yield-scaled N2O emissions compared with conventional fertilization. Our study indicates that both intercropping of white clover and deep application of fertilization without reducing the nitrogen application rate did not reduce the soil N2O emissions in subtropical tea fields. Further studies are needed to determine the effects of deep fertilization application combined with a reduction in the nitrogen application rate on N2O emissions from tea fields.

Design of a low-cost and compact 1 × 5 wavelength-selective switch for access networks.

This paper describes the design, modeling, construction, and testing of a low-cost and compact (80 mm×50 mm) 1×5 wavelength-selective switch. The core beam-deflecting element of the switch is a nematic liquid crystal on silicon spatial light modulator. The switch is designed for coarse wavelength-division multiplexing wavelengths in order to bring the benefit of a low-cost, compact, and robust switching design toward the customer end in the access network. During the system development stage, a single optomechanical assembly was designed and prototyped using the three-dimensional printing technology. The experimental results show an insertion loss of -13.8±1.4 dB and a worst-case scenario crosstalk level of -24.8 dB. Approaches for enhancing the performance of the switch are analyzed and discussed.

Pixel-level fringing-effect model to describe the phase profile and diffraction efficiency of a liquid crystal on silicon device.

We propose a fringing-effect model based on the experimentally measured phase response across the phase transition region of a liquid crystal on silicon (LCOS) device. The measured phase profile in the phase transition region is characterized by a scaled error function of the flyback width. The flyback width can be determined by a cubic function of the phase depth between neighboring pixels. This dependence of the flyback width on the phase depth is explained by a linear rotation model of the liquid crystal director. The simulated diffraction efficiency based on the fringing-effect model shows a close agreement with the experimental measurement.

Broad spectrum measurement of the birefringence of an isothiocyanate based liquid crystal.

Tunable materials with high anisotropy of refractive index and low loss are of particular interest in the microwave and terahertz range. Nematic liquid crystals are highly sensitive to electric and magnetic fields and may be designed to have particularly high birefringence. In this paper we investigate birefringence and absorption losses in an isothiocyanate based liquid crystal (designed for high anisotropy) in a broad range of the electromagnetic spectrum, namely 0.1-4 GHz, 30 GHz, 0.5-1.8 THz, and in the visible and near-infrared region (400 nm-1600 nm). We report high birefringence (Δn = 0.19-0.395) and low loss in this material. This is attractive for tunable microwave and terahertz device applications.