[A new allo-aromadendrane sesquiterpene from Dendrobium nobile].

To study the chemical constituents in the non-alkaloid part of stems of Dendrobium nobile. The macroporous adsorption resin, MCI, silica gel, RP-C_(18), and Sephadex LH-20 gel, preparative thin layer chromatography, and preparative high-performance liquid chromatography(HPLC) were used to isolate and purify the compounds. The structures of the compound were determined according to the spectra data, physicochemical properties, and relevant references. A total of 8 compounds were isolated from D. nobile, which were soltorvum F(1), p-hydroxyphenylpropionic acid(2), vanillic acid(3), p-hydroxybenzoic acid(4), N-trans-cinnamic acid acyl-p-hydroxybenzene ethylamine(5),(+)-(1R,2S,3R,4S,5R,6S,9R)-2,11,12-trihydroxypicrotoxane-3(15)-lactone(6), dendronobilin H(7), soltorvum E(8). Compound 1 was a novel compound, named as soltorvum F. Compound 8 was isolated from Dendrobium species for the first time.

Enhancement from Anaerobic Digestion of Food Waste by Conductive Materials: Performance and Mechanism.

Conductive materials (CM) have recently attracted research interest in the anaerobic digestion of food waste to achieve reduction and resource utilization. Fe-metal organic frameworks (Fe-MOF) and Ketjen Black (KB), the conductive materials (CMs), were added for the enhancement of food waste digestion. This study therefore, is intended to fill in this knowledge gap and clarify the underlying mechanism of CM-promoted performance. Batch experiments revealed that the optimal additions of Fe-MOF and KB were 0.5 g·L-1 and 0.2 g·L-1, respectively. The biogas production increased by 27.50% and 29.45% compared with the blank group, and the removal efficiency of volatile solids (VS), total solids (TS), and chemical oxygen demand (COD) increased by 18.28%, 40.52%, and 15.31%. The lag period was shortened from 3.042 to 2.006 and 1.544 days, respectively. Mechanism studies revealed that Fe-MOF and KB were beneficial to food waste digestion, and the functional groups of Fe-MOF and KB increased the buffer capacity of the system to pH and ammonia nitrogen. The physicochemical properties of Fe-MOF and KB promote the activity of the electron transfer system (ETS); the ETS activity was about 2 times the 11.32 mg·(g·h)-1 of the blank group. Zeta potential and electrical conductivity were beneficial to the establishment of intermicrobial direct interspecies electron transfer (DIET).

Integrating network pharmacology and in vivo experimental validation to reveal the alleviation of mailuoning oral liquid on non-alcoholic fatty liver disease.

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) especially the later stage non-alcoholic steatohepatitis (NASH) seriously endangers human's health and has become a global public health issue in recent years. Mailuoning Oral Liquid (MLN) is a modern traditional Chinese medicine prescription composed by Lonicerae japonicae flos, Achyranthis bidentatae radix, Scrophulariae radix and Dendrobium Caulis. MLN is generally used to treat the syndrome of blood stasis in clinical practice. PURPOSE: To observe the alleviation of MLN on NASH in vivo, and explore the possible underlying mechanism. Furthermore, this study also aims to find which Chinese medicinal drug contained in MLN exerts the main pharmacological activity. METHODS: NASH model was induced in mice by feeding with methionine and choline deficient (MCD) diet. The effects of MLN on hepatic lipids accumulation, liver inflammation, hepatic fibrosis, and the expression of some molecules were investigated by histological observation, biochemical index analysis, quantitative real-time PCR and western blot. Network pharmacology was applied to predict those involved molecular targets and potential mechanisms, which was further validated in vivo. BODIPY fluorescence staining assay was used to detect cellular lipids accumulation. RESULTS: MLN (7.8, 23.4 ml/kg) improved NASH in MCD-fed mice. Network pharmacology results demonstrated that peroxisome proliferator-activated receptor α (PPARα) signaling pathway was crucially involved in the MLN-provided alleviation on NASH. Further experimental validation results showed that MLN increased the expression of peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and restored the decreased expression of nuclear PPARα in MCD-fed mice. Further results displayed that Achyranthis bidentatae radix and Lonicerae japonicae flos contributed greatly to the MLN-provided alleviation on NASH in vivo. BODIPY fluorescence staining assay showed that 25R-inokosterone and cynaroside, two compounds from Achyranthis bidentatae radix and Lonicerae japonicae flos, obviously reduced intracellular lipids accumulation in hepatocytes stimulated by non-esterified fatty acid (NEFA). CONCLUSION: MLN improved NASH in MCD-fed mice, and the PGC-1α-PPARα signaling pathway was involved in this process. Moreover, Lonicerae japonicae flos and Achyranthis bidentatae radix contained in MLN contributed greatly to the MLN-provided improvement on NASH.

An application of slow feature analysis to the genetic sequences of coronaviruses and influenza viruses.

BACKGROUND: Mathematical approaches have been for decades used to probe the structure of DNA sequences. This has led to the development of Bioinformatics. In this exploratory work, a novel mathematical method is applied to probe the DNA structure of two related viral families: those of coronaviruses and those of influenza viruses. The coronaviruses are SARS-CoV-2, SARS-CoV-1, and MERS. The influenza viruses include H1N1-1918, H1N1-2009, H2N2-1957, and H3N2-1968. METHODS: The mathematical method used is the slow feature analysis (SFA), a rather new but promising method to delineate complex structure in DNA sequences. RESULTS: The analysis indicates that the DNA sequences exhibit an elaborate and convoluted structure akin to complex networks. We define a measure of complexity and show that each DNA sequence exhibits a certain degree of complexity within itself, while at the same time there exists complex inter-relationships between the sequences within a family and between the two families. From these relationships, we find evidence, especially for the coronavirus family, that increasing complexity in a sequence is associated with higher transmission rate but with lower mortality. CONCLUSIONS: The complexity measure defined here may hold a promise and could become a useful tool in the prediction of transmission and mortality rates in future new viral strains.

On Time Scales of Intrinsic Oscillations in the Climate System.

Proxy temperature data records featuring local time series, regional averages from areas all around the globe, as well as global averages, are analyzed using the Slow Feature Analysis (SFA) method. As explained in the paper, SFA is much more effective than the traditional Fourier analysis in identifying slow-varying (low-frequency) signals in data sets of a limited length. We find the existence of a striking gap from ~1000 to about ~20,000 years, which separates intrinsic climatic oscillations with periods ranging from ~60 years to ~1000 years, from the longer time-scale periodicities (20,000 year+) involving external forcing associated with Milankovitch cycles. The absence of natural oscillations with periods within the gap is consistent with cumulative evidence based on past data analyses, as well as with earlier theoretical and modeling studies.

[Continuous monitoring of blood adriamycin using a fiber optic chemical sensor in rabbit].

AIM: To evaluate the feasibility and accuracy of continuous monitoring of drug with a fiber optic chemical sensor (FOCS) in animal. METHODS: An accurate optical design was used to enhance the intensity of light from a 100-micron optic fiber and the fluorescence signal could be detected. A new sol-gel method was used to fix the fluorescence substance 4-(N, N-dioctyl) amino-7-nitrobenz-2-oxa-1, 3-diazole (D-70) on the body fiber. The vary quenching means the vary of the concentration of adriamycin (ADM) in rabbit blood. ADM was determined by FOCS based on the fluorescence multiple quenching. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100-micron optic fiber. RESULTS: The recovery of ADM was 99.4%-106.2%, the within-run and between-run RSDs were 6.6%-11.4% and 5.9%-11.7% respectively. The method permitted detection limits as low as 0.057 microgram.mL-1 at a signal-to-noise ratio of 3. CONCLUSION: Fiber-optic chemical sensor is potentially useful for monitoring blood drug in biomedical field.