RESUMEN
Many studies show that dietary factors may affect the risk of nasopharyngeal carcinoma (NPC). We examined the association between overall diet quality and NPC risk in a Chinese population. This case-control study included 600 NPC patients and 600 matched controls between 2009 and 2011 in Guangzhou, China. Habitual dietary intake and various covariates were assessed via face-to-face interviews. Diet quality scores were calculated according to the Healthy Eating Index-2005 (HEI-2005), the alternate Healthy Eating Index (aHEI), the Diet Quality Index-International (DQI-I), and the alternate Mediterranean Diet Score (aMed). After adjustment for various lifestyle and dietary factors, greater diet quality scores on the HEI-2005, aHEI, and DQI-I-but not on the aMed-showed a significant association with a lower risk of NPC (p-trends, <0.001-0.001). The odds ratios (95% confidence interval) comparing the extreme quartiles of the three significant scores were 0.47 (0.32-0.68) (HEI-2005), 0.48 (0.33-0.70) (aHEI), and 0.43 (0.30-0.62) (DQI-I). In gender-stratified analyses, the favorable association remained significant in men but not in women. We found that adherence to the predefined dietary patterns represented by the HEI-2005, aHEI, and DQI-I scales predicted a lower risk of NPC in adults from south China, especially in men.
Asunto(s)
Dieta , Neoplasias Nasofaríngeas/prevención & control , Estado Nutricional , Adulto , Carcinoma , Estudios de Casos y Controles , Distribución de Chi-Cuadrado , China/epidemiología , Dieta/efectos adversos , Conducta Alimentaria , Femenino , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/diagnóstico , Neoplasias Nasofaríngeas/epidemiología , Evaluación Nutricional , Oportunidad Relativa , Factores Protectores , Ingesta Diaria Recomendada , Factores de Riesgo , Factores SexualesRESUMEN
The interaction between colistin sulfate (CS) with bovine serum albumin in physiological buffer (pH 7.4) was investigated with resonance light scattering spectroscopy at 298, 310, and 318 K. The analysis of data indicated that quenching mechanism of BSA-CS was probably static. The value of n was approximately 1, indicating there was only a single class of binding sites on BSA for CS compounds. The thermodynamic parameters were calculated at different temperatures, implying that the interaction was spontaneous and electrostatic force played major role in the binding between CS and BSA. The values of nH were equal to 1 at different temperatures, indicating there was non-cooperative reaction between BSA and CS. The feasibility of resonance light scattering spectroscopy was verified by fluorescence quenching spectroscopy. The quenching reactive parameters (KSVï¼Kqï¼nï¼Ka) from two methods were similar, suggesting resonance light scattering spectroscopy could be used to study the binding interaction between protein and drugs. Resonance light scattering spectroscopy can be used to explore the substance without intrinsic fluorescence, suggesting that the application of resonance light scattering spectroscopy broadens the understanding of the interaction between small molecules and protein.
RESUMEN
BACKGROUND AND OBJECTIVES: Many studies have shown a negative association between the consumption of soy products and the risk of some cancers, but little is known about the effect of soy consumption on nasopharyngeal carcinoma. We assessed the association between the consumption of soy products on nasopharyngeal carcinoma risk in Chinese individuals. METHODS: This case-control study included 600 (448 males and 152 females) incident cases of nasopharyngeal carcinoma, and an equal number of controls, matched according to gender, age (± 3 y) and household type to the nasopharyngeal carcinoma cases. All subjects were recruited from hospitals in Guangzhou, China. A face-to-face interview was conducted with each study individual to collect general information and habitual dietary intake using a 78-item quantitative food-frequency questionnaire. Odds ratios and their 95% confidence intervals were estimated using conditional logistic regression analyses. RESULTS: The median intakes of soy foods (in protein) were 0.5/0.5, 1.4/1.7, 2.7/3.3 and 6.1/7.7 (male/female) g/d in the quartiles 1 to 4. Both univariate and multivariate analyses showed no significant association between the consumption of soy proteins or soy isoflavones and the risk of nasopharyngeal carcinoma. The adjusted odds ratios (95% confidence intervals) between extreme quartiles were 0.97 (0.66-1.45) for soy proteins and 0.97 (0.66-1.42) for total isoflavones. Null associations were also observed between intake of the individual isoflavones daidzein, genistein and glycitein and NPC risk, with adjusted odds ratios for the extreme quartiles ranging between 0.73 and 1.23. CONCLUSION: Habitual consumption of soy products had no significant effect on the risk of nasopharyngeal carcinoma in Chinese adults with a relatively low intake.
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Pueblo Asiatico , Conducta Alimentaria , Neoplasias Nasofaríngeas/etiología , Alimentos de Soja/efectos adversos , Adulto , Anciano , Carcinoma , Estudios de Casos y Controles , China , Demografía , Escolaridad , Femenino , Humanos , Isoflavonas/efectos adversos , Estilo de Vida , Masculino , Persona de Mediana Edad , Carcinoma Nasofaríngeo , Factores de RiesgoRESUMEN
PURPOSE: The effect of fruit and vegetable intake on the risk of nasopharyngeal carcinoma (NPC) remains uncertain due to limited published evidence. We performed a matched case-control study to investigate the relationship between the intake of fruit and vegetables and the risk of NPC. METHODS: Between July 2009 and March 2011, 600 (448 male, 152 female), NPC incident cases from a single hospital in Guangzhou, Guangdong Province, China, a high-incidence area, were enrolled in the study. 600 controls, matched by gender, age (± 3 years) and household type (urban/rural) were also enrolled. Face-to-face interviews were used to collect habitual dietary intakes and information on various covariates. RESULTS: Multivariate conditional logistic regression analyses showed significant, dose-dependent inverse associations between the intake of vegetables, fruit or a combination of the two and the risk of NPC, even after adjustments for social-economic status, body mass index, dietary factors and other potential covariates. The adjusted odds ratios (95% confidence intervals) for NPC in the top quartile of vegetable intake, fruit intake or a combination of the two, as compared to the lowest quartile, were 0.33 (0.22-0.50), 0.70 (0.47-1.04) and 0.37 (0.25-0.55), respectively. Dark green leafy vegetables, carrots, peppers and tomatoes, citrus fruit and pome fruit showed much more pronounced benefits with regards to NPC than other types of fruit and vegetables. Interaction analyses demonstrated that the effects of total combined vegetable and fruit intake were much more significant in subjects with a higher education level (p interaction: 0.027), and the benefits of fruit were observed in males, but not in females (p interaction: 0.088). CONCLUSION: Our findings suggest that a greater consumption of fruit and vegetables may lower the risk of NPC in Chinese adults.
Asunto(s)
Dieta , Frutas , Neoplasias Nasofaríngeas/epidemiología , Verduras , Adulto , Pueblo Asiatico , Carcinoma , Estudios de Casos y Controles , China/epidemiología , Femenino , Humanos , Masculino , Carcinoma Nasofaríngeo , Factores de RiesgoRESUMEN
In the present paper, the fluorescence reaction of cationic surface-active agents (CSAA) with Tetrabromofluorescein sodium (TBF) in aqueous solution was investigated. It was found that the fluorescence quenching of TBF appears when it reacts with the cation monomer of a CSAA and a new stronger fluorescence is obtained when the ion-associates react with the micellate of CSAA. The authors investigated the condition of energy transfer between acidic fluorescent dyes in micelles of cetyl trimethyl ammonium bromide or hexadecyl pyridinium bromide. It was indicated that in the micelles formed by cationic surface active agent with dyes embedded (cationic surface active agent and dyes are charge opposite), the effective energy transfer between anionic dyes could occur. When the concentration of cationic surface active agent reached certain value, the energy transfer could occur. With the value of two thirds of critical micelle concentration, the efficiency of energy transfer reached the maximum; when the concentration of cationic surface active agent increased, the efficiency of energy transfer would be decreased. The authors also deduced the model of energy transfer between dyes in micelles and laws of it.
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A complex composed of ciprofloxacin and terbium (Tb3+) in the solution of acetic acid-sodium acetate buffer (pH 6.2) was studied by fluorescence spectroscopy and ultra-violet absorption spectroscopy. The emission peak of Tb3+ appeared at 490, 545, 590 nm, (the sensitized fluorescence was enforced mightily) and the intensity of 545 nm emission peak was increased obviously. In its acute emission spectrum, the strongest emission peak of Tb3+ was at 545 nm, which was close to the wavelength of the biggest absorption peak of RB, 552 nm. Therefore, as the basic dye rhodamine B(RB) was added, the fluorescence intensity of 545 nm emission peak decreased regularly, indicating that there was a great quenching process. The result showed that the course was statistic. Based on the mechanism of the Förster energy transfer, the efficiency of energy transfer and the distance between the acceptor RB and the complex were found. Thereby, it was indicated that the course of action was single static quenching and the mechanism of quenching was based on energy transfer.
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A hierarchical double-imprinting concept was applied to the preparation of a new organic-inorganic hybrid sorbent for selective separation of Cd(II) from aqueous solution. In the prepared hierarchically imprinted sorbent, both Cd(II) and surfactant micelles (cetyltrimethylammonium bromide) were used as templates. The sorbent was prepared through self-hydrolysis, self-condensation, and co-condensation of the cross-linking agent (tetraethoxysilicate) and the functional precursor (3-(2-aminoethylamino)-propyltrimethoxysilane) in an alkaline media followed by gelation. The selectivity of the sorbent was investigated by a batch competitive ion-binding experiment using an aqueous Cd(II) and Zn(II) mixture. The largest selectivity coefficient for Cd(II) in the presence of Zn(II) was found to be over 100; the largest relative selectivity coefficient between Cd(II) and Zn(II), over 200. The uptake capacity of the prepared hierarchically imprinted sol-gel sorbent and the selectivity coefficient are much higher than those of the sorbent prepared in the absence of CTAB-template. The sorbent possesses a fast kinetics for the removal of Cd(II) from aqueous solution.
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Hydride generation atomic fluorescence spectrometry (HG-AFS) has been used for determination of hydride-forming elements because of its high sensitivity, simplicity, and low costs, but most of such work has been concentrated on single element analysis, and reports dealing with multielement determination by HG-nondispersive (ND)AFS are rare. In this work, a sensitive HG-NDAFS method was developed for simultaneous determination of trace cadmium and arsenic in biological materials. The conditions for the generation of volatile cadmium and arsenic species from the reaction with KBH4 in aqueous solution were investigated using a double-channel AFS integrated with an intermittent flow reactor. Like thiourea and Co(II), ascorbic acid was found to significantly enhance the generation efficiency of volatile Cd and As species. The interferences of coexisting ions were evaluated. Under optimal conditions, the detection limits for Cd and As were determined to be 10 and 150 ng L(-1), respectively. The precision for 11 replicate determinations at the 1 microg L(-1) Cd level and the 10 microg L(-1) As level were 3.5 and 2.7% (RSD), respectively. The recoveries of spike analytes in the biological samples studied ranged from 94 to 109%. The proposed method was successfully applied to the simultaneous determination of Cd and As in a variety of biological samples.