Validation of the Parent Version of the Nighttime Fears Scale (NFS-P) for Children Aged 3-8 Years.

BACKGROUND: Nighttime fears are highly prevalent in children, ranging from normative fears to triggering fear-related anxiety disorders. The lack of available assessment instruments recently prompted the development of the Nighttime Fears Scale (NFS) for children aged 8-12 years. The present study aimed to adapt and psychometrically evaluate the parent-reported version for children aged 3-8 years (NFS-P) as a complement for younger children. METHODS: Two hundred eighty-four Spanish-speaking parents (47% girls) completed the NFS-P and anxiety measures. RESULTS: Confirmatory factor analyses supported a four-factor structure of the NFS-P. Strong internal consistency and validity evidence were obtained. No significant differences were found in NFS-P scores between sexes and age groups. CONCLUSIONS: The findings offer support for the use of the NFS-P as a valuable instrument in clinical and research settings, supplementing the NFS for older children. Both scales provide an efficient means to comprehensively assess the presence and intensity of typical nighttime fears across preschool and school years.

Comparative randomized trial addressing childhood emotional problems: Computerized vs. traditional transdiagnostic Super Skills program.

BACKGROUND: Super Skills for Life is a structured intervention rooted in cognitive-behavioral therapy and a transdiagnostic approach, aimed at preventing childhood emotional problems. Over the past decade, it has demonstrated positive outcomes in various formats and countries. This study evaluated the comparative effectiveness of two individual modalities: the computerized and the traditional version enriched with multimedia content, through a comparative randomized effectiveness trial. METHOD: 109 children (55.24 % female) aged 8-12 years participated, randomly assigned to either the traditional (n = 54) or computerized (n = 51) groups. Pre- and post-intervention assessments involved emotional problem measures completed by children and parents. RESULTS: Both groups exhibited significant reductions in all outcomes according to parent reports, including depression, anxiety, and anxiety-related life interference. Children's self-reports indicated improvements across all general measures. Generalized Estimating Equations indicated marginally better improvements in the traditional group. LIMITATIONS: Limitations included the absence of follow-up assessment, variability in participant regions across Spain, and input from other informants like teachers. CONCLUSIONS: This study pioneers the examination of short-term effects of the program in both a fully computerized format supervised by a specialized therapist and an enriched traditional individual format, and the promising results suggest their potential for indicated prevention of childhood emotional problems.

Assessing family relations in borderline personality disorder: A relational approach.

The aims of the current study are to describe the basic family relationships, parental bonding patterns, and dyadic adjustment of families with offspring diagnosed with borderline personality disorder (BPD) and to explore the correlations between these variables related to family relations and BPD symptomatology. The sample consisted of 194 participants, including parents from the control (N = 76) and clinical group (N = 76), and patients with BPD (N = 42). All progenitors completed a measure of family relations, parental bonding, and dyadic adjustment. Patients completed a measure of parental bonding and borderline symptomatology. The results showed significant differences between both groups in marital and parental functioning, marital satisfaction, dyadic adjustment, and care. Correlations among family variables and BPD symptomatology were also found. In summary, findings underscore the significance of comprehending the complexity of family relationships in BPD while advocating for a relational perspective when examining the family dynamics.

A brief parent version of the Spence Children's Anxiety Scale (SCAS-P-8): Factor structure, reliability, and invariance in Ecuadorian children / Validación de la versión para padres de la Escala de Ansiedad Infantil de SpenceBreve (SCAS-P-8) en niños ecuatorianos

Abstract Objective: The present study aimed to assess the psychometric properties and factor structure of the parent-reported brief version of the Spence Children's Anxiety Scale (SCAS-P-8) in a sample of Ecuadorian children. Method: The community sample consisted of 281 children from 8 to 12 years of age and their parents. Factor structure of the scale was assessed through confirmatory factor analysis. The psychometric properties and sex and age invariance were calculated. Results: The results showed that the one-factor structure of the SCAS-P-8 provided a good fit for the data, and that the structure was invariant across gender and age, indicating that it is suitable for assessing anxiety symptoms in both boys and girls of different ages with comparable accuracy. Psychometric analysis revealed a total ordinal alpha of .85, and corrected item-total correlations varied from .41 to .61, indicating an acceptable level of homogeneity. Conclusion: The study found that the SCAS-P-8 is a psychometrically reliable tool for measuring anxiety in Ecuadorian children from 8 to 12 years of age, as reported by their parents, and can be used in both research and clinical settings to evaluate anxiety symptoms in community populations in Ecuador. Given its brief and accurate nature, the SCAS-P-8 is considered to be a useful tool for use in both research and clinical settings for evaluating anxiety symptoms in children in this country.

Resumen Objetivo: Este artículo tiene por objetivo evaluar las propiedades psicométricas y la estructura factorial de la versión breve de la Escala de Ansiedad Infantil de Spence (SCAS-P-8) informada por los padres en una muestra de niños ecuatorianos. Método: La muestra comunitaria estuvo compuesta por 281 niños de entre 8 y 12 años y sus padres. La estructura factorial de la escala fue evaluada mediante análisis factorial confirmatorio. Se calcularon las propiedades psicométricas y la invarianza en función del género y la edad. Resultados: Los resultados mostraron que la estructura unifactorial de la escala proporcionó un buen ajuste para los datos, y que esta fue invariante en cuanto al género y la edad. Esto indica que el SCAS-P-8 es una medida adecuada para evaluar los síntomas de ansiedad tanto en niños como en niñas de diferentes edades con una precisión comparable. El análisis psicométrico reveló un alfa ordinal total de .85, y las correlaciones corregidas ítem-total variaron de .41 a .61, indicando un nivel aceptable de homogeneidad. Conclusión: La escala SCAS-P-8 es una herramienta adecuada para medir la ansiedad en niños ecuatorianos de 8 a 12 años por medio de la información reportada por sus padres. Dada su naturaleza breve y precisa, el SCAS-P-8 se considera una herramienta útil para su uso tanto en investigaciones como en entornos clínicos para evaluar los síntomas de ansiedad en niños de Ecuador.

Factor Structure and Psychometric Properties of the Spence Children's Anxiety Scale: A 25-Year Systematic Review.

The Spence Children's Anxiety Scale (SCAS) is one of the most used instruments to assess anxiety symptoms in children and adolescents. Extensive research has been conducted to examine its psychometric properties and to develop other versions of the scale. The objective of this study was to examine the psychometric properties and factorial structure of the SCAS across different versions and populations. This systematic review followed PRISMA guidelines and was registered. APA PsycINFO, Web of Science (Core Collection) and MEDLINE (PubMed) were searched. Fifty-two studies were included in this systematic review. They examined the factor structure, convergent and divergent validity, and internal consistency of the scale. The most supported model was the original six-factor model, followed by the higher order six-factor model for the long version of the SCAS. Studies provided evidence of convergent validity and internal consistency. It is concluded that the SCAS is a valid and reliable instrument for assessing anxiety symptoms in children and adolescents, with a six-factor model structure well supported in most populations. Further research on the psychometric properties and factor structure of other versions of the scale and its application to clinical populations is warranted.

Factors Associated with Post-Traumatic Stress Symptoms in Pregnant and Postpartum Women.

Background: Evidence of post-traumatic stress disorder (PTSD) symptoms related to the COVID-19 pandemic during the perinatal period and the associated risk factors are still limited. Thus, we aimed to investigate the PTSD symptoms associated with the COVID-19 pandemic in a large sample of both pregnant and postpartum women. Methods: A cross-sectional study was conducted on 3319 pregnant and up to 6-month postpartum women from Spain. An online survey was completed between June 2020 and January 2021. The assessment included measures of PTSD symptoms associated with COVID-19 (evaluated with 10 questions from the PTSD checklist for Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition), pandemic-related concerns and health background (assessed by the Coronavirus Perinatal Experiences-Impact Survey), and demographic characteristics. Results: We found that >40% of women suffered from symptoms of PTSD associated with the COVID-19 pandemic. Difficulty concentrating and irritability were the most common symptoms, showing marked alterations in arousal and reactivity associated with the traumatic event. Being younger, suffering from pandemic concerns and distress, changes due to the pandemic and previous mental health problems were risk factors associated with PTSD symptoms in perinatal women. In addition, whereas being an immigrant (non-Spanish) was a risk factor for pregnant women, having other children and financial problems were risk factors for postpartum women. COVID-19 infection did not appear to be a risk factor for symptoms of PTSD in perinatal women. Conclusions: The increased risk of PTSD in pregnant and postpartum women highlights the importance of early detection and treatment of PTSD for pregnant and postnatal women, both during and beyond the pandemic. Trial Registration: ClinicalTrials.gov Identifier (NCT04595123).

Exploring the psychological impact of COVID-19 on adolescents with borderline personality disorder and their mothers: A focus group study.

The aim of this study was to explore the impact of the coronavirus pandemic on adolescents diagnosed with Borderline Personality Disorder (BPD) and their mothers. This exploratory study used a qualitative focus group approach. This study's sample group consisted in nine participants: five adolescents diagnosed with BPD and their four mothers. Patients were recruited from a specialized BPD outpatient unit of a university hospital psychiatry department. The results are divided into two main areas, the first regarding the lockdown period and the second examining the period of gradual relaxation of lockdown restrictions. The results show that the adolescents had difficulties in the management of their interpersonal relationships, especially in striking a balance between individual and family space, as well as in communication, cohesion, and family dynamics. During the COVID lockdown, adolescents experienced a stabilization of psychopathological symptoms, but these symptoms worsened when the lockdown restrictions were lifted. Nevertheless, they reported having learned and implemented self-care strategies. The findings are discussed in terms of both individual and family impact, shedding light on some of the challenges precipitated by the COVID-19 pandemic.

The couple's tree of life: Promoting and protecting relational identity.

We present The Couple's Tree of Life (CTOL) as a new collective narrative methodology to strengthen couple relationships and prevent conflicts. The CTOL, based on the tree of life methodology (Ncube & Denborough, Tree of Life, mainstreaming psychosocial care and support: a manual for facilitators, REPSSI, 2007), aims to reinforce the identity and strengths of the couple. We explain the CTOL implementation process and illustrate it step by step with a group of 14 adult heterosexual Caucasian couples who belonged to Protestant churches in Madrid (Spain). As a way to assess its usefulness before applying the CTOL to other groups of couples, we conducted a pre-post evaluation using the Dyadic Adjustment Scale of Spanier(1976). We found an improvement in dyadic adjustment, quality, understanding of, and satisfaction with, the relationship. The results, though not generalizable at this stage, suggest that the CTOL could reinforce the couple's identity while maintaining individual identities. We also discuss the possible applications of couples therapy.

Presentamos El árbol de la vida de la pareja (CTOL, por sus siglas en inglés) como una nueva metodología narrativa colectiva, con el fin de fortalecer las relaciones de pareja y para la prevención de conflictos. El CTOL, basado en la metodología del árbol de la vida (Ncube & Denborough, Tree of Life, mainstreaming psychosocial care and support: a manual for facilitators, REPSSI, 2007), tiene como finalidad reforzar la identidad y las fortalezas de la pareja. Explicamos el proceso de implementación del CTOL y lo ejemplificamos paso a paso con un grupo de 14 parejas heterosexuales caucásicas adultas que pertenecían a iglesias protestantes en Madrid (España). Como forma de evaluar su utilidad antes de aplicar el CTOL a otros grupos de parejas, realizamos una evaluación previa y posterior utilizando la Escala de Ajuste Diádico de Spanier (1976). Hallamos una mejora en el ajuste diádico, en la calidad, en la comprensión de la relación y en la satisfacción con esta. Los resultados, aunque no son generalizables en esta etapa, sugieren que el árbol de la vida de la pareja podría reforzar la identidad de la pareja y a la vez mantener las identidades individuales. También explicamos las posibles aplicaciones a la terapia de pareja.

Recombinant Protein-Based Nanoparticles: Elucidating their Inflammatory Effects In Vivo and their Potential as a New Therapeutic Format.

Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein in a mechanically stable nanostructured format that has been broadly characterized, showing promising potential in different fields such as tissue engineering, protein replacement therapies, cancer, and biotechnology. For immunomodulatory purposes, however, the interference of the format immunogenic properties-intrinsic to IBs-with the specific effects of the therapeutic protein is still an uncovered gap. For that, active and inactive forms of the catalytic domain of a matrix metalloproteinase-9 (MMP-9 and mutMMP-9, respectively) have been produced as IBs and compared with the soluble form for dermal inflammatory effects in mmp9 knock-out mice. After protein injections in air-pouches in the mouse model, MMP-9 IBs induce local neutrophil recruitment and increase pro-inflammatory chemokine levels, lasting for at least two days, whereas the effects triggered by the soluble MMP-9 format fade out after 3 h. Interestingly, the IB intrinsic effects (mutMMP-9 IBs) do not last more than 24 h. Therefore, it may be concluded that IBs could be used for the delivery of therapeutic proteins, such as immunomodulating proteins while preserving their stability in the specific tissue and without triggering important unspecific inflammatory responses due to the protein format.

Physiological responses to salinity in the yellow-horned poppy, Glaucium flavum.

Glaucium flavum Crantz. is a short-lived perennial herb found in coastal habitats in southern Spain growing under a wide range of interstitial soil salinity levels, from that of fresh water up to the high concentration typical of sea water. An experiment was designed to investigate the effect of exposure to this range of salinity on the photosynthetic apparatus, growth and reproduction of G. flavum, by measuring relative growth rate, percentage of dead leaves, seed production, leaf relative water content, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total sodium, potassium, calcium, magnesium, and nitrogen concentrations. G. flavum survived at NaCl concentrations as high as 300 mM, although the excess of NaCl resulted in a biomass reduction of between 26 and 76% (in 60 and 300 mM NaCl treatments, respectively). The long-term effects of salinity on the growth and reproduction of G. flavum were mainly linked to an overall reduction in carbon gain as a result of stomatal conductance regulation. Also, the excess of salt caused a reduction in pigment concentrations, as well as Ca-, Mg- and N-uptake. The results indicate that, in the presence of excess soil-water salinity, G. flavum sustains little overall effects on the photochemical (PSII) apparatus, and is capable of tolerating a very high and continued exposure to salinity by maintaining low levels of net photosynthesis.

Carry-over of differential salt tolerance in plants grown from dimorphic seeds of Suaeda splendens.

BACKGROUND AND AIMS: Halophytic species often show seed dimorphism, where seed morphs produced by a single individual may differ in germination characteristics. Particular morphs are adapted to different windows of opportunity for germination in the seasonally fluctuating and heterogeneous salt-marsh environment. The possibility that plants derived from the two morphs may also differ physiologically has not been investigated previously. METHODS: Experiments were designed to investigate the germination characteristics of black and brown seed morphs of Suaeda splendens, an annual, C(4) shrub of non-tidal, saline steppes. The resulting seedlings were transferred to hydroponic culture to investigate their growth and photosynthetic (PSII photochemistry and gas exchange) responses to salinity. KEY RESULTS: Black seeds germinated at low salinity but were particularly sensitive to increasing salt concentrations, and strongly inhibited by light. Brown seeds were unaffected by light, able to germinate at higher salinities and generally germinated more rapidly. Ungerminated black seeds maintained viability for longer than brown ones, particularly at high salinity. Seedlings derived from both seed morphs grew well at high salinity (400 mol m(-3) NaCl). However, seedlings derived from brown seeds performed poorly at low salinity, as reflected in relative growth rate, numbers of branches produced, F(v)/F(m) and net rate of CO(2) assimilation. CONCLUSIONS: The seeds most likely to germinate at high salinity in the Mediterranean summer (brown ones) retain a requirement for higher salinity as seedlings that might be of adaptive value. On the other hand, black seeds, which are likely to delay germination until lower salinity prevails, produce seedlings that are less sensitive to salinity. It is not clear why performance at low salinity, later in the life cycle, might have been sacrificed by the brown seeds, to achieve higher fitness at the germination stage under high salinity. Analyses of adaptive syndromes associated with seed dimorphism may need to take account of differences over the entire life cycle, rather than just at the germination stage.

Growth and photosynthetic responses to salinity of the salt-marsh shrub Atriplex portulacoides.

BACKGROUND AND AIMS: Atriplex (Halimione) portulacoides is a halophytic, C(3) shrub. It is virtually confined to coastal salt marshes, where it often dominates the vegetation. The aim of this study was to investigate its growth responses to salinity and the extent to which these could be explained by photosynthetic physiology. METHODS: The responses of young plants to salinity in the range 0-700 mol m(-3) NaCl were investigated in a glasshouse experiment. The performance of plants was examined using classical growth analysis, measurements of gas exchange (infrared gas analysis), determination of chlorophyll fluorescence characteristics (modulated fluorimeter) and photosynthetic pigment concentrations; total ash, sodium, potassium and nitrogen concentrations, and relative water content were also determined. KEY RESULTS: Plants accumulated Na(+) approximately in proportion to external salinity. Salt stimulated growth up to an external concentration of 200 mol m(-3) NaCl and some growth was maintained at higher salinities. The main determinant of growth response to salinity was unit leaf rate. This was itself reflected in rates of CO(2) assimilation, which were not affected by 200 mol m(-3) but were reduced at higher salinities. Reductions in net photosynthetic rate could be accounted for largely by lower stomatal conductance and intercellular CO(2) concentration. Apart from possible effects of osmotic shock at the beginning of the experiment, salinity did not have any adverse effect on photosystem II (PSII). Neither the quantum efficiency of PSII (Phi(PSII)) nor the chlorophyll fluorescence ratio (F(v)/F(m)) were reduced by salinity, and lower mid-day values recovered by dawn. Mid-day F(v)/F(m) was in fact depressed more at low external sodium concentration, by the end of the experiment. CONCLUSIONS: The growth responses of the hygro-halophyte A. portulacoides to salinity appear largely to depend on changes in its rate of photosynthetic gas exchange. Photosynthesis appears to be limited mainly through stomatal conductance and hence intercellular CO(2) concentration, rather than by effects on PSII; moderate salinity might stimulate carboxylation capacity. This is in contrast to more extreme halophytes, for which an ability to maintain leaf area can partially offset declining rates of carbon assimilation at high salinity.

Genome sequence and organization of a nucleopolyhedrovirus isolated from the smaller tea tortrix, Adoxophyes honmai.

Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) has a distinctive pathology in A. honmai larvae, killing the host more slowly than other NPVs. To further understand the pathology of AdhoNPV, its genome was completely sequenced and compared with those of other baculoviruses. The AdhoNPV genome is 113,220 bp, with a G + C content of 35.6%. It contains 125 putative open reading frames (ORFs), of which 8 are unique to AdhoNPV, and 4 homologous regions. The other 117 ORFs display similarity to previously characterized baculovirus genes involved in early and late gene expression, DNA replication, and structural and auxiliary functions. The phylogenetic position of AdhoNPV, in relation to 15 other baculoviruses whose genomes have been completely sequenced, was assessed by three different analyses: gene sequence, gene order, and gene content. Although gene content analysis failed to support the group II NPVs, phylogenetic trees based on gene sequence and gene order showed AdhoNPV to be closely related to the group II NPVs.

Sequence analysis of a 5.1 kbp region of the Spodoptera frugiperda multicapsid nucleopolyhedrovirus genome that comprises a functional ecdysteroid UDP-glucosyltransferase (egt) gene.

The ecdysteroid UDP-glucosyltransferase gene from the Spodoptera frugiperda multicapsid nucleopolyhedrovirus (SfMNPV) was identified using degenerate primers whose sequence were derived from conserved regions of the EGT proteins encoded by other baculoviruses. Analysis of the gene sequence revealed the presence of an open reading frame (ORF) with potential to encode a polypeptide of 525 amino acids. Promoter sequences typical of baculovirus genes were found in the 5' region of this ORF. A polyadenylation signal was identified downstream the translation stop codon. A transient expression assay showed that the product of this ORF was able to conjugate glucose from UDP-glucose with ecdysone confirming that the gene identified was indeed the SfMNPV egt gene. The SfMNPV egt gene and the sequences of other baculovirus egt genes were used to infer a phylogenetic tree. The nucleotide sequence of the entire BamHI fragment that contains the SfMNPV egt gene was determined. Search of the available sequence databases suggested that, besides the egt gene, this region contains 5 ORFs similar to the baculovirus genes gp37 (fusolin), to ptp2 and to ORFs 28, 29, and 30 of Spodoptera exigua multicapsid nucleopolyhedrovirus. Both the phylogenetic analysis of the egt genes and the gene order of the region that flanks the egt gene indicated that SfMNPV is closely related to the baculoviruses that infects S. exigua and Mamestra configurata.

Functional and phylogenetic analyses of a putative Drosophila melanogaster UDP-glycosyltransferase gene.

Glucosidation plays a major role in the inactivation and excretion of a great variety of both endogenous and exogenous compounds. The recent determination of the complete genome sequence of Drosophila melanogaster has revealed the presence of over 30 putative UDP-glucosyltransferase (UGT) genes in this organism. We report here the molecular cloning and functional characterisation of one of these genes, named DmUgt37a1. The predicted protein comprises 525 amino acids and has about 30% overall amino acid identity with vertebrate members of the UGT family. The phylogenetic relationships of DmUgt37a1 with other members of the UGT family from D. melanogaster are discussed. DmUgt37a1 was expressed in lepidopteran insect cells and the ability of the enzyme to conjugate 38 potential substrates belonging to diverse chemical groups was assessed using UDP-glucose as sugar-donor. However, no activity was detected with any compound under the conditions used and thus, the substrate specificity of the enzyme remains unknown.

Characterization of a novel silkworm (Bombyx mori) phenol UDP-glucosyltransferase.

Sugar conjugation is a major pathway for the inactivation and excretion of both endogenous and exogenous compounds. We report here the molecular cloning and functional characterization of a phenol UDP-glucosyltransferase (UGT) from the silkworm, Bombyx mori, which was named BmUGT1. The complete cDNA clone is 1.6 kb, and the gene is expressed in several tissues of fifth-instar larvae, including fat body, midgut, integument, testis, silk gland and haemocytes. The predicted protein comprises 520 amino acids and has approximately 30% overall amino-acid identity with other members of the UGT family. The most conserved region of the protein is the C-terminal half, which has been implicated in binding the UDP-sugar. BmUGT1 was expressed in insect cells using the baculovirus expression system, and a range of compounds belonging to diverse chemical groups were assessed as potential substrates for the enzyme. The expressed enzyme had a wide substrate specificity, showing activity with flavonoids, coumarins, terpenoids and simple phenols. These results support a role for the enzyme in detoxication processes, such as minimizing the harmful effects of ingested plant allelochemicals. This work represents the first instance where an insect ugt gene has been associated with a specific enzyme activity.

The complete sequence of the Cydia pomonella granulovirus genome.

The nucleotide sequence of the DNA genome of Cydia pomonella granulovirus (CpGV) was determined and analysed. The genome is composed of 123500 bp and has a G+C content of 45.2%. It contains 143 ORFs of 150 nucleotides or more that show minimal overlap. One-hundred-and-eighteen (82.5%) of these putative genes are homologous to genes previously identified in other baculoviruses. Among them, 73 are homologous to genes of Autographa californica nucleopolyhedrovirus (AcMNPV), whereas 108 and 98 are homologous to genes of Xestia c-nigrum GV (XcGV) and Plutella xylostella GV (PxGV), respectively. These homologues show on average 37.4% overall amino acid sequence identity to those from AcMNPV and 45% to those from XcGV and PxGV. The CpGV gene content was compared to that of other baculoviruses. Several genes reported to have major roles in baculovirus biology were not found in the CpGV genome, such as gp64, the major budded virus glycoprotein gene in some nucleopolyhedroviruses, and lef-7, involved in DNA replication. However, the CpGV genome encodes the large and small subunits of ribonucleotide reductase, three inhibitor of apoptosis (iap) homologues and two protein tyrosine phosphatases. The CpGV, PxGV and XcGV genomes present a noticeably high level of conservation of gene order and orientation. A striking feature of the CpGV genome is the absence of typical homologous repeat sequences. However, it contains one major repeat region and 13 copies of a single 73-77 bp imperfect palindrome.