A novel electrochemical biosensor based on MIL-101-NH2 (Cr) combining target-responsive releasing and self-catalysis strategy for p53 detection.

A novel electrochemical biosensor was constructed to detect p53 gene based on MIL-101-NH2 (Cr) by combining target-responsive releasing and self-catalysis strategy. MIL-101-NH2 (Cr) with suitable pore structure was used to encapsulate methylene blue (MB) as signal probe. The hairpin DNA (HP) containing rich-G sequences was used as gatekeeper to seal up the pores and avoid MB leakage through covalent immobilization. The p53 gene could hybridize with the loop portion of HP for the formation of dsDNA, which had the specific nicking site of the nicking endonuclease (Nt.BstNBI). Then Nt.BstNBI recognized the specific recognition site and cleaved HP to open the pore for releasing of MB. Meanwhile, the cleavage of HP released the target DNA to trigger the target recycling for signal amplification. More importantly, the plentiful rich-G sequences were exposed to form Hemin/G-quadruplex DNAzymes, which could unite MIL-101-NH2 (Cr) to catalyze redox reaction of MB released by itself for signal amplification. The biosensor for p53 had wide linear range from 1 × 10-14 to 1 × 10-7 M and a low detection limit of 1.4 × 10-15 M. The combination of target-responsive releasing and self-catalysis strategy provided a promising way for constructing ultrasensitive and simple biosensor.

Aptamer-functionalized metal-organic frameworks (MOFs) for biosensing.

As a class of crystalline porous materials, metal-organic frameworks (MOFs) have attracted increasing attention. Due to the nanoscale framework structure, adjustable pore size, large specific surface area, and good chemical stability, MOFs have been applied widely in many fields such as biosensors, biomedicine, electrocatalysis, energy storage and conversions. Especially when they are combined with aptamer functionalization, MOFs can be utilized to construct high-performance biosensors for numerous applications ranging from medical diagnostics and food safety inspection, to environmental surveillance. Herein, this article reviews recent innovations of aptamer-functionalized MOFs-based biosensors and their bio-applications. We first briefly introduce different functionalization methods of MOFs with aptamers, which provide a foundation for the construction of MOFs-based aptasensors. Then, we comprehensively summarize different types of MOFs-based aptasensors and their applications, in which MOFs serve as either signal probes or signal probe carriers for optical, electrochemical, and photoelectrochemical detection, with an emphasis on the former. Given recent substantial research interests in stimuli-responsive materials and the microfluidic lab-on-a-chip technology, we also present the stimuli-responsive aptamer-functionalized MOFs for sensing, followed by a brief overview on the integration of MOFs on microfluidic devices. Current limitations and prospective trends of MOFs-based biosensors are discussed at the end.