Comprehensive analysis of the role of a four-gene signature based on EMT in the prognosis, immunity, and treatment of lung squamous cell carcinoma.

Epithelial-mesenchymal transition (EMT), a biological process through which epithelial cells transform into mesenchymal cells, contributes to tumor progression and metastasis. However, a comprehensive analysis of the role of EMT-related genes in Lung squamous cell carcinoma (LUSC) is still lacking. In this study, data were downloaded from available databases, including The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) database. The association between differentially expressed EMT-related genes (EMT-RDGs) and LUSC prognosis, drug sensitivity, mutation, and immunity was analyzed using bioinformatics methods. In the results, Lasso and univariate Cox regression analyses identified four EMT-RDGs that were differentially expressed, and used to establish a prognostic model capable of distinguishing between high- and low-risk groups. Then, prognostic factors were identified by multivariate Cox regression analysis and used to construct a nomogram. The high-risk group had a significantly poorer prognosis than the low-risk group. The tumor immune environment was significantly different between the two groups, with the low-risk group exhibiting a better response to immunotherapy. In addition, the half-maximal inhibitory concentration prediction indicating that the constructed model could effectively predict sensitivity to chemotherapy. This study provides new reference for further exploration of new clinical therapeutic strategies for LUSC.

Role of the regulation of mesenchymal stem cells on macrophages in sepsis.

Sepsis is a common clinical critical disease with high mortality. The excessive release of cytokines from macrophages is the main cause of out-of-control immune response in sepsis. Mesenchymal stem cells (MSCs) are thought to be useful in adjunctive therapy of sepsis and related diseases, due to their function in immune regulation, anti-inflammatory, antibacterial, and tissue regeneration. Also there have been several successful cases in clinical treatment. Some previous studies have shown that MSCs regulate the function of macrophages through secreting cytokines and extracellular vesicles, or transferring mitochondria directly to target cells, which affects the progress of sepsis. Here, we review the regulation of MSCs on macrophages in sepsis, mainly focus on the regulation ways. We hope that will help to understand the immunological mechanism and also provide some clues for the clinical application of MSCs in the biotherapy of sepsis.

Energy Consumption, Carbon Emissions and Global Warming Potential of Wolfberry Production in Jingtai Oasis, Gansu Province, China.

During the last decade, China's agro-food production has increased rapidly and been accompanied by the challenge of increasing greenhouse gas (GHG) emissions and other environmental pollutants from fertilizers, pesticides, and intensive energy use. Understanding the energy use and environmental impacts of crop production will help identify environmentally damaging hotspots of agro-production, allowing environmental impacts to be assessed and crop management strategies optimized. Conventional farming has been widely employed in wolfberry (Lycium barbarum) cultivation in China, which is an important cash tree crop not only for the rural economy but also from an ecological standpoint. Energy use and global warming potential (GWP) were investigated in a wolfberry production system in the Yellow River irrigated Jingtai region of Gansu. In total, 52 household farms were randomly selected to conduct the investigation using questionnaires. Total energy input and output were 321,800.73 and 166,888.80 MJ ha-1, respectively, in the production system. The highest share of energy inputs was found to be electricity consumption for lifting irrigation water, accounting for 68.52%, followed by chemical fertilizer application (11.37%). Energy use efficiency was 0.52 when considering both fruit and pruned wood. Nonrenewable energy use (88.52%) was far larger than the renewable energy input. The share of GWP of different inputs were 64.52% electricity, 27.72% nitrogen (N) fertilizer, 5.07% phosphate, 2.32% diesel, and 0.37% potassium, respectively. The highest share was related to electricity consumption for irrigation, followed by N fertilizer use. Total GWP in the wolfberry planting system was 26,018.64 kg CO2 eq ha-1 and the share of CO2, N2O, and CH4 were 99.47%, 0.48%, and negligible respectively with CO2 being dominant. Pathways for reducing energy use and GHG emission mitigation include: conversion to low carbon farming to establish a sustainable and cleaner production system with options of raising water use efficiency by adopting a seasonal gradient water pricing system and advanced irrigation techniques; reducing synthetic fertilizer use; and policy support: smallholder farmland transfer (concentration) for scale production, credit (small- and low-interest credit) and tax breaks.

The Bet-Hedging Strategies for Seedling Emergence of Calligonum mongolicum to Adapt to the Extreme Desert Environments in Northwestern China.

Calligonum mongolicum is a dominant native perennial shrub on sand dunes in arid deserts of northwestern China, and is therefore widely used in sand dune stabilization in these regions. However, it remains largely unknown how seedling emergence of C. mongolicum has adapted to unpredictable sand movement and extreme drought. Here we examined effects of seed burial depth, light intensity, and seed age on seedling emergence, and considered seed germination and seedling emergence strategies for the shrub's adaption to the desert environment. In our pot experiment, the optimum seeding depth for emergence of C. mongolicum was 2 cm, indicating that for germination and seedling emergence only moderate sand burial is required. Light intensity at the surface soil (0 cm) was important for seedling emergence, while there was no significant difference between 50 and 20% light flux density, at burial depths of 1 and 2 cm, indicating that C. mongolicum seeds had adapted to sand burial, while not exposure from sand erosion. We also found C. mongolicum seedlings emerged in spring and in late summer to early autumn. Meanwhile, seedling emergence percentage for 3-year-old seeds was similar to that of 1-year-old seeds, which meant that C. mongolicum seeds were well preserved under normal sand dune conditions, thus were capable of developing a persistent, but shallow soil seed-bank. These results indicated that germination and seedling emergence take a bet-hedging strategies to adapt to variable desert environments. Our study confirmed that C. mongolicum desert shrubs combine strategies in its adaption to arid and variable sand environments.

Factors influencing the natural regeneration of the pioneering shrub Calligonum mongolicum in sand dune stabilization plantations in arid deserts of northwest China.

Calligonum mongolicum is a successful pioneer shrub to combat desertification, which is widely used for vegetation restoration in the desert regions of northwest China. In order to reveal the limitations to natural regeneration of C. mongolicum by asexual and sexual reproduction, following the process of sand dune stabilization, we assessed clonal shoots, seedling emergence, soil seed bank density, and soil physical characteristics in mobile and stabilized sand dunes. Controlled field and pot experiments were also conducted to assess germination and seedling emergence in different dune soil types and seed burial depths. The population density of mature C. mongolicum was significantly different after sand dune stabilization. Juvenile density of C. mongolicm was much lower in stabilized sand dunes than mobile sand dune. There was no significant difference in soil seed bank density at three soil depths between mobile and stabilized sand dunes, while the emergence of seedlings in stabilized dunes was much lower than emergence in mobile dunes. There was no clonal propagation found in stabilized dunes, and very few C. mongolicum seedlings were established on stabilized sand dunes. Soil clay and silt content, air-filled porosity, and soil surface compaction were significantly changed from mobile sand dune to stabilized dunes. Seedling emergence of C. mongolicm was highly dependent on soil physical condition. These results indicated that changes in soil physical condition limited clonal propagation and seedling emergence of C. mongolicum in stabilized sand dunes. Seed bank density was not a limiting factor; however, poor seedling establishment limited C. mongolicum's further natural regeneration in stabilized sand dunes. Therefore, clonal propagation may be the most important mode for population expansion in mobile sand dunes. As a pioneer species C. mongolicum is well adapted to propagate in mobile sand dune conditions, it appears unlikely to survive naturally in stabilized sand dune plantations.

Changes in wind erosion over a 25-year restoration chronosequence on the south edge of the Tengger Desert, China: implications for preventing desertification.

Wind erosion is a primary cause of desertification as well as being a serious ecological problem in arid and semi-arid areas across the world. To determine mechanisms for restoring desertified lands, an unrestored shifting sand dune and three formerly shifting sand dunes (desertified lands) that had been enclosed and afforested for 5, 15, and 25 years were selected for evaluation on the south edge of the Tengger Desert, China. Based on sampling heights between 0.2 and 3 m, the critical threshold average wind speed was 6.5 m s-1 at 2 m where the sand transport rate was reduced from 285.9 kg m-2 h-1 on the unrestored dunes to 9.1 and 1.8 kg m-2 h-1 on the sites afforested and enclosed for 5 and 15 years, respectively. The percentage of wind eroded area was reduced from 99.9% on the unrestored dune to 94.5, 9.0, and 0.5% on the sites afforested and enclosed for 5, 15, and 25 years, respectively. Wind erosion was effectively reduced after 15 years. Although there were different driving factors for wind erosion mitigation on the different restoration stages, an increase in the vegetation cover, surface roughness, soil shear strength, soil clay content, organic matter, and reduction in the near-surface wind speed were the primary variables associated with the restoration chronosequence. We conclude that reducing the wind speed and developing a biological crust through vegetation restoration were the critical components for restoration of desertified land.

Long-Term Effects of Xerophytic Shrub Haloxylon ammodendron Plantations on Soil Properties and Vegetation Dynamics in Northwest China.

The xerophytic desert shrub Haloxylon ammodendron (C. A. Mey.) Bunge. is distributed naturally in Asian and African deserts, and is widely used for vegetation restoration in the desert regions of Northern China. However, there are limited long-term chrono-sequence studies on the impact of changed soil properties and vegetation dynamics following establishment of this shrub on mobile sand dunes. In Minqin County, Gansu Province, we investigated soil properties and herbaceous vegetation development of 10, 20, 30, 40, 50-year-old H. ammodendron plantations on mobile sand dunes. Soil sampling at two depths (0-5 and 5-20 cm) under the shrubs determined SOC, nutrition and soil physical characteristics. The results showed that: establishment of H. ammodendron had improved soil physio-chemical properties, increased thickness of soil crusts and coverage of biological soil crusts (BSCs), and promoted development of topsoil over an extended period of 5 decades. Soil texture and soil nutrition improved along the chrono-sequence according to three distinct phases: i) an initial fast development from 0 to 10 years, ii) a stabilizing phase from 10 to 30 years followed by iii) a relatively marked restoration development in 40 and 50-year-old plantations. Meanwhile, herbaceous community coverage also markedly increased in 30-year-old plantations. However, both soil and vegetation restoration were very slow due to low annual precipitation in Minqin county compared to other Northern China sand afforestation sites. Canonical Correspondence Analysis results demonstrated that herbaceous plant development was closely associated with changes in soil texture (increased clay and silt percentage) and availability of soil nutrients. Thus our results indicated that selection of the long-lived shrub H. ammodendron is an essential and effective tool in arid desert re-vegetation.

Carbon benefits of wolfberry plantation on secondary saline land in Jingtai oasis, Gansu--A case study on application of the CBP model.

The largest global source of anthropogenic CO2 emissions comes from the burning of fossil fuel and approximately 30% of total net emissions come from land use and land use change. Forestation and reforestation are regarded worldwide as effective options of sequestering carbon to mitigate climate change with relatively low costs compared with industrial greenhouse gas (GHG) emission reduction efforts. Cash trees with a steady augmentation in size are recognized as a multiple-beneficial solution to climate change in China. The reporting of C changes and GHG emissions for sustainable land management (SLM) practices such as afforestation is required for a variety of reasons, such as devising land management options and making policy. The Carbon Benefit Project (CBP) Simple Assessment Tool was employed to estimate changes in soil organic carbon (SOC) stocks and GHG emissions for wolfberry (Lycium barbarum L.) planting on secondary salinized land over a 10 year period (2004-2014) in the Jingtai oasis in Gansu with salinized barren land as baseline scenario. Results show that wolfberry plantation, an intensively managed ecosystem, served as a carbon sink with a large potential for climate change mitigation, a restorative practice for saline land and income stream generator for farmers in soil salinized regions in Gansu province. However, an increase in wolfberry production, driven by economic demands, would bring environmental pressures associated with the use of N fertilizer and irrigation. With an understanding of all of the components of an ecosystem and their interconnections using the Drivers-Pressures-State-Impact-Response (DPSIR) framework there comes a need for strategies to respond to them such as capacity building, judicious irrigation and institutional strengthening. Cost benefit analysis (CBA) suggests that wolfberry cultivation was economically profitable and socially beneficial and thus well-accepted locally in the context of carbon sequestration. This study has important implications for Gansu as it helps to understand the role cash trees can play in carbon emission reductions. Such information is necessary in devising management options for sustainable land management (SLM).