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1.
J Toxicol Sci ; 38(6): 847-54, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24213004

RESUMEN

The purpose of the present study was to collect the background data on Wistar Hannover [Crl:WI(Han)] (hereafter Wistar Han) rats in embryo-fetal development studies from the 6 safety research facilities of pharmaceutical companies and contract research organizations. In each facility, 20 or 22 female rats were dosed with vehicle solution during the organogenesis period. As a result, no abnormalities in clinical signs and necropsy findings in dams were found. Body weights and food consumption in dams were lower than those in Sprague Dawley (SD) rats. The number of corpora lutea (13.3 vs. 16.0 in SD) and implantations (11.8 vs. 14.7) were fewer, and fetal body weights (3.66 vs. 3.70) and placental weights (0.42 vs. 0.45) tended to be lower than those in SD rats. Regarding the fetal abnormalities, the incidence of several findings such as the persistent left umbilical artery (10.4% vs. 1.1%) and cervical (5.2% vs. 0.4%), full (7.4% vs. 0.9%) or short supernumerary (64.5% vs. 9.9%) and wavy ribs (6.6% vs. 0.3%) was higher than that in SD rats. Our present study showed that they maintained a sufficient number of live fetuses and the difference in the fetal sex ratio was not observed. In conclusion, Wistar Han rats were considered to be a suitable strain for embryo-fetal development toxicity study. Since the incidence of several abnormalities was higher than that in SD rats, it may be said that to accumulate background control data is important to evaluate the embryo-fetal development toxicity study using Wistar Han rats.


Asunto(s)
Desarrollo Fetal , Modelos Animales , Anomalías Musculoesqueléticas/embriología , Anomalías Musculoesqueléticas/epidemiología , Ratas Sprague-Dawley , Ratas Wistar , Pruebas de Toxicidad , Toxicología/métodos , Vísceras/anomalías , Vísceras/embriología , Animales , Peso Corporal , Cuerpo Lúteo , Ingestión de Alimentos , Implantación del Embrión , Femenino , Peso Fetal , Tamaño de los Órganos , Organogénesis , Placenta/anatomía & histología , Embarazo , Ratas
2.
J Toxicol Sci ; 38(6): 855-73, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24213005

RESUMEN

The purpose of the present study was to collect background data from repeated dose toxicity studies in Wistar Hannover [Crl:WI(Han)] (hereafter Wistar Han) rats with dosing periods of 4, 13 and 26 weeks from four safety research facilities of pharmaceutical companies and contract research organizations participating in the International Genetic Standardization (IGS) rat forum supported by Charles River Laboratories Japan, Inc. The data from Wistar Han rats were compared with those from Sprague Dawley Crl:CD(SD) rats. In addition, the effects of restricted feeding of SD rats were also investigated by one facility. As a result, body weights and food consumption in Wistar Han rats were lower than those of SD rats. White blood cell (WBC), neutrophil, lymphocyte, monocyte and eosinophil counts were almost half of those noted for SD rats and platelet counts were almost 20% less than those in SD rats. Minimal strain differences were noted in several biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase, total cholesterol, triglyceride and phospholipids, and in thymus, ovary and testis weights. Ophthalmologic or histopathologic examinations revealed a higher incidence of corneal opacities or corneal mineralization in Wistar Han rats. Restricted feeding of SD rats resulted in intermediate values for body weights and food consumption between the ad libitum fed SD and Wistar Han rats, and WBC and AST were lower than those in the ad libitum fed SD rats. Based on these results, some strain differences might be ascribable to reduced food consumption and associated body weight changes in Wistar Han rats.


Asunto(s)
Peso Corporal , Ingestión de Alimentos , Modelos Animales , Ratas Sprague-Dawley , Ratas Wistar , Pruebas de Toxicidad , Toxicología/métodos , Alanina Transaminasa/sangre , Animales , Aspartato Aminotransferasas/sangre , Recuento de Células Sanguíneas , Opacidad de la Córnea/epidemiología , Femenino , Lípidos/sangre , Masculino , Tamaño de los Órganos , Ovario , Ratas , Testículo , Timo
3.
J Appl Toxicol ; 31(1): 63-74, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-20677212

RESUMEN

The murine local lymph node assay (LLNA) is a well-established alternative to the guinea pig maximization test (GPMT) or Buehler test (BT) for the assessment of the skin sensitizing ability of a drug, cosmetic material, pesticide or industrial chemical. Instead of radioisotope using in this method, Takeyoshi M. et al. (2001) has developed a modified LLNA based on the 5-bromo-2'-deoxyuridine (BrdU) incorporation (LLNA:BrdU-ELISA). The LLNA:BrdU-ELISA is practically identical to the LLNA methodology excluding the use of BrdU, for which a single intraperitoneal injection of BrdU is made on day 4, and colorimetric detection of cell turnover. We conducted the validation study to evaluate the reliability and relevance of LLNA:BrdU-ELISA. The experiment involved 7 laboratories, wherein 10 chemicals were examined under blinded conditions. In this study, 3 chemicals were examined in all laboratories and the remaining 7 were examined in 3 laboratories. The data were expressed as the BrdU incorporation using an ELISA method for each group, and the stimulation index (SI) for each chemical-treated group was determined as the increase in the BrdU incorporation relative to the concurrent vehicle control group. An SI of 2 was set as the cut-off value for exhibiting skin sensitization activity. The results obtained in the experiments conducted for all 10 chemicals were sufficiently consistent with small variations in their SI values. The sensitivity, specificity, and accuracy of LLNA:BrdU-ELISA against those of GPMT/BT were 7/7 (100%), 3/3 (100%), and 10/10 (100%), respectively.


Asunto(s)
Bromodesoxiuridina/metabolismo , Ensayo del Nódulo Linfático Local , Compuestos Orgánicos/análisis , Animales , Dermatitis Alérgica por Contacto/diagnóstico , Dermatitis Alérgica por Contacto/etiología , Ensayo de Inmunoadsorción Enzimática , Femenino , Laboratorios , Ratones , Ratones Endogámicos CBA , Compuestos Orgánicos/toxicidad , Control de Calidad , Distribución Aleatoria , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
4.
J Pharmacol Toxicol Methods ; 58(1): 11-26, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-18593646

RESUMEN

INTRODUCTION: The murine local lymph node assay (LLNA) is a well-established alternative to the guinea pig maximization test (GPMT) or Buehler test (BT) for the assessment of the skin sensitizing ability of drugs and chemicals. Daicel Chemical Industries Ltd. has developed a modified LLNA based on the adenosine triphosphate (ATP) content (LLNA-DA). We conducted 2 interlaboratory validation studies to evaluate the reliability and relevance of LLNA-DA. METHODS: The experiment involved 17 laboratories, wherein 14 chemicals were examined under blinded conditions. In the first study, 3 chemicals were examined in 10 laboratories and the remaining 9 were examined in 3 laboratories. In the second study, 1 chemical was examined in 7 laboratories and the remaining 4 chemicals were examined in 4 laboratories. The data were expressed as the ATP content for each chemical-treated group, and the stimulation index (SI) for each chemical-treated group was determined as the increase in the ATP content relative to the concurrent vehicle control group. An SI of 3 was set as the cut-off value for exhibiting skin sensitization activity. RESULTS: The results of the first study obtained in the experiments conducted for the 3 chemicals that were examined in all the 10 laboratories and for 5 of the remaining 9 chemicals were sufficiently consistent with small variations in their SI values. The sensitivity, specificity, and accuracy of LLNA-DA against those of GPMT/BT were 7/8 (87.5%), 3/3 (100%), and 10/11 (90.9%), respectively. In the second study, all the 5 chemicals studied demonstrated acceptably small interlaboratory variations. DISCUSSION: In the first study, a large variation was observed for 2 chemicals; in the second study, this variation was small. It was attributed to the application of dimethylsulfoxide as the solvent for the metallic salts. In conclusion, these 2 studies provide good evidence for the reliability of the LLNA-DA.


Asunto(s)
Dermatitis Alérgica por Contacto/etiología , Irritantes/toxicidad , Ensayo del Nódulo Linfático Local , Adenosina Trifosfato/metabolismo , Animales , Dermatitis Alérgica por Contacto/diagnóstico , Femenino , Ratones , Ratones Endogámicos CBA , Reproducibilidad de los Resultados , Solventes/química , Pruebas de Toxicidad/métodos
5.
J Toxicol Sci ; 29(5): 497-504, 2004 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-15729005

RESUMEN

Regulatory and industrial scientists collaborated to publish a "points to consider" document regarding the safety assessment of biotechnology-derived pharmaceuticals in non-clinical studies in 2002 (Pharmaceutical Non-clinical Investigation Group, 2002). The collaboration team intended to clarify the interpretation of ICH-S6 guideline and furthermore share recent Japanese practices on this matter. However, the document was written in Japanese. Thus, we share here an English translation of the document so that non-native Japanese correctly understand the contents.


Asunto(s)
Productos Biológicos/toxicidad , Evaluación Preclínica de Medicamentos/métodos , Industria Farmacéutica/legislación & jurisprudencia , Animales , Productos Biológicos/administración & dosificación , Productos Biológicos/farmacocinética , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos/normas , Guías como Asunto , Humanos , Cooperación Internacional , Japón , Pruebas de Toxicidad/métodos
6.
Nihon Yakurigaku Zasshi ; 119(3): 175-84, 2002 Mar.
Artículo en Japonés | MEDLINE | ID: mdl-11915520

RESUMEN

Levocabastine is a selective histamine H1-receptor antagonist exerting inhibitory effects on the release of chemical mediators from mast cells and on the chemotaxis of polymorphonuclear leukocytes and eosinophils. Both histamine and antigens induced conjunctivitis was inhibited by levocabastine in several allergy models. Levocabastine moderately inhibited histamine-release from guinea pig conjunctive induced by antigen-antibody reactions and prevented an increase in the vascular permeability of the conjunctive elicited by both histamine and antigen instillation. Symptoms of allergic rhinitis, which were induced by histamine, substance P and antigen, were also reduced by levocabastine. Levocabastine prevented an increase in the vascular permeability of nasal mucosa elicited by instillation of these three inducers. Furthermore, levocabastine has shown a large difference between the antiallergic dose and other non-specific pharmacological effective dose than that with other antiallergic drugs. The non-specific pharmacological effect of levocabastine reveals only blepharoptosis. With these pharmacological effects and topical usage, levocabastine was shown to be useful for allergic conjunctive and rhinitis in both seasonal and perennial clinical use.


Asunto(s)
Antagonistas de los Receptores Histamínicos H1/farmacología , Antagonistas de los Receptores Histamínicos H1/uso terapéutico , Piperidinas/farmacología , Piperidinas/uso terapéutico , Animales , Quimiotaxis de Leucocito/efectos de los fármacos , Ensayos Clínicos como Asunto , Conjuntivitis Alérgica/tratamiento farmacológico , Depresión Química , Modelos Animales de Enfermedad , Eosinófilos/fisiología , Antagonistas de los Receptores Histamínicos H1/farmacocinética , Liberación de Histamina/efectos de los fármacos , Humanos , Mastocitos/metabolismo , Neutrófilos/fisiología , Piperidinas/farmacocinética , Rinitis Alérgica Perenne/tratamiento farmacológico
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