RESUMEN
The main objectives of this work were to characterise a range of purified recombinant sterol 3ß-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3ß-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active domain of the Saccharomyces cerevisiae 3ß-glucosyltransferase was used to mine putative sterol 3ß-glucosyltransferases from the databases. Selected diverse sequences were expressed in and purified from Escherichia coli and shown to have different selectivities for the 3ß-hydroxysteroids ergosterol and cholesterol. Surprisingly, three enzymes were also selective for testosterone, a 17ß-hydroxysteroid. This study therefore reports for the first time sterol 3ß-glucosyltransferases with selectivity for both 3ß- and 17ß-hydroxysteroids and is also the first report of recombinant 3ß-glucosyltransferases with selectivity for steroids with a hydroxyl group at positions other than C-3. These enzymes could therefore find utility in the pharmaceutical industry for the green synthesis of a range of glycosylated compounds of medicinal interest.
Asunto(s)
Glucosiltransferasas/metabolismo , Esteroles/metabolismo , Testosterona/metabolismo , Secuencia de Aminoácidos , Catálisis , Dominio Catalítico , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Glucosiltransferasas/química , Glucosiltransferasas/genética , Cinética , Datos de Secuencia Molecular , Dominios y Motivos de Interacción de Proteínas , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Alineación de Secuencia , Especificidad por SustratoRESUMEN
The biosynthesis of the complex carbohydrates that govern many cellular functions requires the action of a diverse range of selective glycosyltransferases (GTs). Uridine diphosphate sugar-utilizing GTs (UGTs) account for the majority of characterized GTs. GTs have been classified into families (currently 92) based on amino-acid sequence similarity. However, as amino-acid sequence similarity cannot reliable predict catalytic mechanism, GTs have also been grouped into four clans based on catalytic mechanism and structural fold. GTs catalyze glycosidic bond formation with two possible stereochemical outcomes: inversion or retention of anomeric configuration. All UGTs also belong to one of two distinct structural folds, GT-A and GT-B. UGTs have conserved residues that are associated with nucleotide diphosphate sugar recognition and acceptor recognition. UGT diversification has been performed using in vitro DNA recombination, domain swapping, and random mutagenesis.
