AhR agonists screening and identification in indoor dust based on non-target chemical analysis by GC-Q-TOFMS and biological effect evaluation referring to ToxCast/Tox21 database.

Numerous studies reported the concentration of agonists of aryl hydrocarbon receptor (AhR) in indoor dust by target chemical analysis or the biological effects of activating the AhR by indoor extracts, but the major AhR agonists identification in indoor dust were rarely researched. In the present study, the indoor dust samples were collected for 7-ethoxyresorufin O-deethylase (EROD) assay and both non-targeted and targeted chemical analysis for AhR agonists by gas chromatography quadrupole time-of-flight mass spectrometry and gas chromatography-mass spectrometry analysis. Coupled with non-targeted analysis and toxicity Forecaster (ToxCast)/Tox21 database, 104 ToxCast chemicals were screened to be able to induce EROD response. The combination of targeted chemical analyses and biological effects evaluation indicated that PAHs, dibutyl phthalate (DBP) and Cypermethrin might be the important AhR-agonists in different indoor dust and mainly contributed in 1.84%-97.56 % (median: 26.62%) of total observed biological effects through comparing toxic equivalency quotient derived from chemical analysis with biological equivalences derived from bioassay. DBP and cypermethrin seldom reported in the analysis of AhR agonists should raise great concern. In addition, the present results in experiment of synthetic solution of 4 selected AhR-agonists pointed out that some unidentified AhR agonists existed in indoor dust.

Applications of charcoal, activated charcoal, and biochar in aquaculture - A review.

Environmental pollution and poor feed quality pose potential threats to aquatic organisms and human health, representing challenges for the aquaculture industry. In light of the rising demand for aquatic organisms, there is an urgent need to improve aquacultural production and protect the products from contamination. Char, a carbonaceous material derived through pyrolysis of organic carbon-rich biomass, has proven advantages in soil, air, and water remediation. While char's performance and the associated physicochemical characteristics depend strongly on the pyrolysis temperature, residence time, and feedstock type, char generally shows advantages in pollutant removal from the environment and livestock. This enables it to enhance the health and growth performance of livestock. Given the growing attention to char application in aquaculture in recent years, this review summarises major studies on three applications: aquacultural water treatment, sediment remediation, and char-feed supplement. Most of these studies have demonstrated char's positive effects on pollutant removal from organisms and aquacultural environments. Moreover, adopting char as fish feed can improve fish growth performance and the condition of their intestinal villi. However, due to insufficient literature, further investigation is needed into the mechanistic aspects of pollutants removal in aquatic organisms by char as a feed additive, such as the transportation of char inside aquatic organisms, the positive and negative effects of char on these products, and how char alters the gut microbiota community of these products. This paper presents an overview of the current application of char in aquaculture and highlights the research areas that require further investigation to enrich future studies.

Human health risk assessment based on a total diet study of daily mercury intake in Chengdu, China.

To assess the total daily mercury intake and main exposure sources of residents, six food groups, including marine fish, freshwater fish, poultry, livestock, vegetables, and cereals, were collected from five districts of Chengdu, China. The median concentrations of total mercury (THg) and methylmercury (MeHg) were 12.8 and 6.94 µg kg-1 ww, respectively. Cereals (32.2%), vegetables (30.5%), and livestock (16.2%) contributed to a much larger extent to the total consumption for the participants in Chengdu. All food categories that contributed the most of THg (2.16 µg day-1) and MeHg 1.44 (µg day-1) to the daily intake in Chengdu were cereals and marine fish, respectively. The total Hazard Ratios values below 1 in this study indicate that there is no health risk associated with Hg ingestion from the consumption of these foods for the residents in Chengdu.

Effects of sodium selenite, yeast selenium, and nano-selenium on toxicity, growth, and selenium bioaccumulation in Lucilia sericata maggots.

In this study, we investigated the effects of different types of selenium (Se) (sodium selenite [SS], yeast selenium [YS], and nano-selenium [NS]) on the toxicity, growth, Se accumulation, and transformation of Lucilia sericata maggots (LSMs). We found that the 50% lethal concentration of LSMs exposed to SS was 2.18 and 1.96 times that of YS and NS, respectively. LSM growth was significantly promoted at exposure concentrations of 10-50 mg kg-1 in group SS and 10-30 mg kg-1 in group YS, whereas NS inhibited LSMs growth at all concentrations (p < 0.05). Total Se content in LSMs, conversion efficiency to organic and other forms of Se, and bioaccumulation factor of Se were the highest in the SS group when exposed to 50 mg kg-1 (81.6 mg kg-1, 94.6%, and 1.63, respectively). Transcriptomic results revealed that LSMs significantly upregulated the amino acid (alanine, aspartate, glutamic, and tyrosine) and tricarboxylic acid cycle signaling pathways (p < 0.05) on exposure to Se, resulting in a significant increase in LSMs biomass and quality. In conclusion, our study indicates that LSMs exhibit good tolerance to SS and can convert it into bioorganic or other forms of Se.

Ecological and human health hazards of soil heavy metals after wildfire: A case study of Liangshan Yi autonomous prefecture, China.

Soil samples were collected in at different depths from the conflagration area in Liangshan Yi Autonomous Region, China, to investigate the distribution characteristics and ecological and human health risks of heavy metals after a wildfire. The samples collected comprise wildfire ash (WA) above the soil surface, ash soil (AS) 0-5 cm, and plain soil (PS) 5-15 cm below the soil surface. Additionally, reference soil (RS) was collected from a nearby unburned area at the same latitude as the conflagration area. The results showed that the concentrations of zinc (Zn), copper (Cu), lead (Pb), and cadmium (Cd) in the WA and AS were significantly higher than in reference soil (RS) (p < 0.05). Concentrations of Pb in the PS were 2.52 times higher than that in RS (17.9 mg kg-1) (p < 0.05). The AS and WA had the highest Index of potential ecological risks (RI > 600). In addition, The Cd in AS and WA contributed the most to the highest Improved nemerow index (INI) and RI with a contribution of more than 80%. The concentration of heavy metals was used to establish non-carcinogenic effects and cancer risks in humans via three exposure pathways: accident ingestion of soil, dermal contact with soil, and inhalation of soil particles. Hazard index (HI) values of each sample were all less than 1, indicating the non-carcinogenic risk was within the acceptable range and would not adversely affect the local population's health. The Cancer risk (CR) values of Cr, As, Cd, and Ni were all below 1 × 10-6, indicating that heavy metal pollution from this wildfire did not pose a cancer risk to residents.

Microplastics removal mechanisms in constructed wetlands and their impacts on nutrient (nitrogen, phosphorus and carbon) removal: A critical review.

Microplastics (MPs) are now prevalent in aquatic ecosystems, prompting the use of constructed wetlands (CWs) for remediation. However, the interaction between MPs and CWs, including removal efficiency, mechanisms, and impacts, remains a subject requiring significant investigation. This review investigates the removal of MPs in CWs and assesses their impact on the removal of carbon, nitrogen, and phosphorus. The analysis identifies crucial factors influencing the removal of MPs, with substrate particle size and CWs structure playing key roles. The review highlights substrate retention as the primary mechanism for MP removal. MPs hinder plant nitrogen uptake, microbial growth, community composition, and nitrogen-related enzymes, reducing nitrogen removal in CWs. For phosphorus and carbon removal, adverse effects of MPs on phosphorus elimination are observed, while their impact on carbon removal is minimal. Further research is needed to understand their influence fully. In summary, CWs are a promising option for treating MPs-contaminated wastewater, but the intricate relationship between MPs and CWs necessitates ongoing research to comprehend their dynamics and potential consequences.

Per- and polyfluoroalkyl substances (PFASs) in Chinese surface water: Temporal trends and geographical distribution.

PFAS, recognized as persistent organic pollutants, present risks to both the ecological environment and human health. Studying PFASs in surface water yields insights into pollution dynamics. However, existing research on PFASs surface water pollution in China often focuses on specific regions, lacking comprehensive nationwide analyses. This study examined 48 research papers covering PFAS pollution in Chinese surface water, involving 49 regions and 1338 sampling sites. The results indicate widespread PFAS contamination, even in regions like Tibet. Predominant PFAS types include PFOA and PFOS, and pollution is associated with the relocation of industries from developed to developing countries post-2010. The shift from long-chain to short-chain PFASs aligns with recent environmental policy proposals. Geographic concentration of PFAS pollution correlates with industry distribution and economic development levels. Addressing point source pollution, especially from wastewater plant tailwater, is crucial for combating PFAS contamination. Greater emphasis should be placed on addressing short-chain PFASs.

Hair Heavy Metals and Food Consumption in Residents of Chengdu: Factors, Food Contribution, and Health Risk Assessment.

Heavy metal pollution is one of the most pressing issues threatening food security and human health. This study assesses heavy metal (chromium, cadmium, copper, zinc, nickel, and lead) exposure via hair metal concentrations in Chengdu residents, reflecting metal intake from food consumption. From June 2020 to February 2021, a sampling survey was conducted on residents' hair (n=182) and food (n=301) in six main urban areas of Chengdu. The concentrations of heavy metals in hair and food were analyzed by inductively coupled plasma mass spectrometry, and the results showed that the residents of Chengdu City had high hair concentrations of Cd (0.17±0.03 mg kg-1) and Zn (293±21.3 mg kg-1). Gender significantly affected the hair Cr, Zn, and Ni concentrations. Based on the survey results obtained from Chengdu City residents, the habits and diet structure are assessed for the influence of six heavy metals in the hair of the residents. Adolescents' (13-18 years old) hair had significantly higher Pb concentrations than adults (19-59 years old). The concentration of Ni in hair was affected by perming and dyeing habits. For dietary exposure, cereals and meat were the main contributors to the residents' daily intake of heavy metals. The bioaccessibility of Cr, Cd, Cu, Zn, Ni, and Pb in food was 2.45-74.67%, 10.6-78.7%, 13.4-82.5%, 8.89-89.2%, 7.70-85.1%, and 15.4-86.2%, respectively. In health risk evaluation based on the bioaccessible fraction of six heavy metals, the hazard quotient of each heavy metal in food was less than 1, indicating no potential non-carcinogenic risk.

MgFe-LDH@biochars for removing ammonia nitrogen and phosphorus from biogas slurry: Synthesis routes, composite performance, and adsorption mechanisms.

Layered double hydroxide-biochar composites (LDH@BCs) have been developed for ammonia nitrogen (AN) and phosphorus (P) removal from wastewater. Improvement of LDH@BCs was limited due to the lack of comparative evaluation based on LDH@BCs characteristics and synthetic methods and information on the adsorption properties of LDH@BCs for N and P from natural wastewater. In this study, MgFe-LDH@BCs were synthesized by three different co-precipitation procedures. The differences in physicochemical and morphological properties were compared. They were then employed to remove AN and P from biogas slurry. The adsorption performance of the three MgFe-LDH@BCs was compared and evaluated. Different synthesis procedures can significantly affect the physicochemical and morphological characteristics of MgFe-LDH@BCs. The LDH@BC composite fabricated through a novel method (labeled 'MgFe-LDH@BC1') has the largest specific surface area, Mg and Fe content, and excellent magnetic response performance. Moreover, the composite has the best adsorption property of AN and P from biogas slurry (30.0% and 81.8%, respectively). The main reaction mechanisms include memory effect, ion exchange, and co-precipitation. Applying 2% MgFe-LDH@BC1 saturated with AN and P adsorption from biogas slurry as a fertilizer substitute can substantially improve soil fertility and increase plant production by 139.3%. These results indicate that the facile LDH@BC synthesis method is an effective method to overcome the shortcomings of LDH@BC in practical application, and provide a basis for further exploration of the potential application of biochar based fertilizers in agriculture.

Selenium toxicity and bioaccumulation in selenium-enriched fly (Chrysomya megacephala) maggots.

Selenium (Se) is an essential trace element for human health, and as a potential animal feed, the Chrysomya megacephala (Fabricius) fly is rich in protein and fat. By using different concentrations of sodium selenite (0, 30, 50, 70 mg kg-1), the possibility of biological Se enrichment in C. megacephala (Fabricius) maggots (CMMs) was investigated. The accumulation, Se speciation, enzymatic activity, and concentrations of copper (Cu), zinc (Zn), chromium (Cr), and cadmium (Cd) in the maggots were also determined. Transcriptomics was also used to investigate the mechanism of the Se response to CMM genes. The results showed that the CMMs had a survival rate of > 80% at Se exposure concentrations ranging from 0 to 100 mg kg-1. The optimal concentration of sodium selenite for CMM growth was 50 mg kg-1, and the weight, protein content, and total Se accumulation of the larvae (10.8 g, 53.5%, and 72.6 ± 3.36 mg kg-1 (DW), respectively) were considerably higher than the control and other exposure doses (p < 0.05). In addition, Se improved the ability of maggots to absorb Cu and Zn, decreased malondialdehyde (MDA) and lipid peroxidation, but improved the antioxidant activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX). Furthermore, Se negatively affected the absorption of Cd and Cr. According to the transcriptomic findings, Se supplementation can boost protein synthesis and control both antioxidant and non-antioxidant enzyme activity in CMMs. Therefore, our findings showed that Se-enriched CMMs may counteract the toxicity of Cd and Cr, and Se is an effective supplement for improving the consumption safety of cultured animals fed containing CMMs.

Using fly larvae to convert food waste for growing Oujiang color common carps: health risk assessment of polycyclic aromatic hydrocarbons.

The present study used Chrysomya megacephala larvae (CML) to transform food waste into safe and high-quality fish feed to substitute fish meal as a source of protein for growing Oujiang color common carps followed by a human health risk assessment of polycyclic aromatic hydrocarbons (PAHs). The results showed the ∑PAH concentration in the CML fed with food waste ranged from 50 to 370 µg kg-1, and the most abundant PAH compound in the CML was BaP, contributing 59-84% of ∑PAHs. The Pearson correlation analysis results indicated no correlation between the ∑PAH concentrations and the culture substrate ratio (p > 0.05). Concentrations of BaP in the CML decreased with the increase of breeding density (p < 0.01). The residues as organic fertilizers have no potential ecological risk for PAHs. The biotransformed larva meal was used to partially or completely replace the fish meal as supplementary protein in the experimental feeds (T0, 0%; T50, 50%; T100, 100%). No significant difference (p < 0.05) of survival rate, lipid, and protein content in Oujiang color common carp was noted among T0, T50, and T100 fish feeds. Concentrations of ∑PAHs in Oujiang color common carp fed with the CML fish feeds all met the food safety standards in the European Union (EU). Furthermore, the consumption of Oujiang color common carps fed with the CML feed does not pose any health risks of PAHs for humans.

Influence of exogenous selenomethionine and selenocystine on uptake and accumulation of Se in winter wheat (Triticum aestivum L. cv. Xinong 979).

Soil selenium (Se) is mainly inorganic including selenate and selenite but organic forms such as selenomethionine (SeMet) and selenocystine (SeCys2) are commonly present. Although organic Se is bioavailable or potentially bioavailable to plants, whether the effects of the organic Se on uptake and accumulation of Se in winter wheat differ in forms is still not clear. Both hydroponic experiments and a pot trial of whole plant growth stage were conducted to investigate the effects of SeMet and L-selenocystine (SeCys2) on uptake and accumulation of Se in winter wheat (Triticum aestivum L. cv. Xinong 979). Not only metabolic inhibitor (carbonyl cyanide m-chlorophenylhydrazone (CCCP)) inhibited SeMet (44%) influx into wheat roots but also aquaporin inhibitor (AgNO3) or putative inhibitor (H2SiO4 and H3BO3) suppressed 83%, 62%, or 64% SeMet influx into the roots. However, these inhibitors had insignificant effects on SeCys2 influx into the roots. Wheat grain possessed more effective Se accumulation under SeCys2 treatments than under SeMet treatments, which was contributed to more efficiently translocation of Se from husk to grain, more remobilization of tissue Se to grain, and significantly higher concentration of soluble Se (SOL-Se) and exchangeable and carbonate-bound Se (EXC-Se) in the rhizosphere of winter wheat. The present study indicated that the effects of organic Se on uptake and accumulation of Se in winter wheat differed in forms and that SeCys2 exhibited the potential to increase grain Se concentration in winter wheat. The results from the present study will replenish information about the effects and related mechanisms of SeMet or SeCys2 on uptake and accumulation of Se in winter wheat and provide insights of effects of organic Se on wheat grain Se accumulation.

Health risk assessment of mercury in Nile tilapia (Oreochromis niloticus) fed housefly maggots.

The bioaccumulation of total mercury (THg) and methylmercury (MeHg) by housefly maggots (HM) during the conversion of food waste (vegetables and meat (VM) and rice waste) under various waste feed ratios were investigated. Subsequently, Nile tilapia (Oreochromis niloticus) were fed with the commercial feed, commercial dried HM, dried HM, and fresh HM, followed by a human health risk assessment of Hg via fish consumption. The THg concentrations of HM fed with food waste ranged from 39.5 to 100 µg kg-1 ww. Concentrations of MeHg in the maggots fed with 100 % vegetables and meat (VM) waste (13.7 ± 1.12 µg kg-1 ww) was significantly higher than that fed with other mixed ratios of rice waste and VM waste (p<0.05). Concentrations of MeHg were positively correlated with the weight and lipid content of houseflies (p<0.05). THg and MeHg concentrations in tilapia fed with the converted HM (dried and fresh HM) were 22.5 ± 6.50 µg kg-1 ww and 2.43 ± 0.36 µg kg-1 ww, respectively. There was no significant difference in MeHg between tilapia fed the four experiment diets (p>0.05). Health risk assessment results indicated that mercury in tilapia fed the food waste-grown HM did not pose potential health risks to humans (target hazard quotient < 1). In conclusion, HM could convert food waste into high-quality and safe fish feeds for cultivating tilapia.

Using food waste to cultivate safe, good-quality Sabah (giant hybrid) grouper: Dioxins and dioxin-like polychlorinated biphenyls.

Dioxins and dioxin-like polychlorinated biphenyls (dl-PCBs) in fish fillet counteract the health benefits of fish products. In this study, food waste was used as a protein alternative to replacing fishmeal commonly used in the commercial fish feed, aiming to cultivate Sabah grouper with acceptable levels of dioxins and dl-PCBs. The concentrations of dioxins and dl-PCBs, as well as the fish growth performance, were compared between the fish groups fed with food waste-based feed (FWBF) and commercial feed (Nanyu®, control). The results showed that the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) (1.22 pg/g dry weight (d.w.)) and non-ortho-dl-PCBs (13.0 pg/g d.w.) were significantly lower (p < 0.05) in the FWBF than in the control feed (commercial feed) (PCDDs: 2.35 pg/g d.w.; non-ortho-dl-PCBs: 27.2 pg/g d.w.). The growth performance of the fish group fed with the FWBF was comparable to that fed with the control feed. There were no significant differences between the WHO2005-TEQ values of different fish fillets (1.00, 1.11, and 1.10 pg WHO2005-TEQ/g d.w. for FWBF group, control feed group, and local market fish, respectively). Based on the guidelines provided by European Food Safety Authority (ESFA) and U.S. Environmental Protection Agency (USEPA), the fish fed with the FWBF were safe for human consumption (hazard index values: 0.284-0.522; cancer risk range: 2.59-2.97 × 10-5). The findings of this study suggest that food waste could serve as an alternative protein source for cultivating Sabah grouper with acceptable levels of dioxins and dl-PCBs.

Selenium uptake and accumulation in winter wheat as affected by level of phosphate application and arbuscular mycorrhizal fungi.

Selenium (Se) is an advantageous element to crops. However, the influence of arbuscular mycorrhizal fungi (AMF), phosphate (P) and selenite in soil on Se uptake by winter wheat remain elusive. Pot trials were carried out including seven levels of P (0, 12.5, 25, 50, 100, 200 or 400 mg kg-1) and non-mycorrhizal inoculation (NM), inoculation of Funneliformis mosseae (F.m) or Glomus versiforme (G.v). The present results found that grain phosphorus concentration increased with increase of P level from 0 to 100 mg kg-1 and then tended to plateau, while grain Se concentration decreased with the level of P from 0 to 400 mg kg-1. Based on mathematical modeling, inoculation of F.m or G.v dramatically improved grain Se concentration by 16.90% or 12.53% under the lower level of P (48.76 mg kg-1). Furthermore, partial least squares path modeling (PLS-PM) identified that both up-regulated of the expression of AMF-inducible phosphate transporter and improved Se bioavailability in rhizosphere soil contributed to enhancing plant Se concentration under P levels ≤ 100 mg kg-1. The present study demonstrated that AMF combined with 48.76 mg kg-1 P applied in soil can not only achieve high grain yield, but also fully exploit the biological potential of Se uptake in wheat.

Environmental health impacts of microplastics exposure on structural organization levels in the human body.

The ubiquitous prevalence of microplastics pollution has raised concerns about microplastics' potential risks and impacts on the global environment. However, the potential human health risks and impacts of microplastics remain largely unexplored. By providing an overview regarding the interaction of microplastics and human health, this review extends current knowledge on the potential impacts of microplastics pollution on humans from an environmental health perspective. The paper firstly presents the characteristics of microplastics as well as the status of global microplastics pollution. As for human health, the potential hazards of microplastics are reflected by toxic chemical components, vectors of contaminants, and physical damage. Extensive microplastic pollution on ecosystems due to human activities leads to inevitable human exposure, which may occur by dietary, inhalation and/or skin contact. Accordingly, microplastics exposure is closely associated with human health. This study explores the potential interactions of microplastics with the biological organization at various levels, including chemical, cellular, tissue, organ, and system levels. The review concludes by highlighting five urgent perspectives and implications for future research on microplastics: 1) Developing a standard terminology and research methods; 2) Reinforcing microplastics pollution governance; 3) Exploring innovative strategies and technologies; 4) Engaging the public and change behaviour; and 5) Adopting a transdisciplinary approach.

Size-dependent transformation, uptake, and transportation of SeNPs in a wheat-soil system.

Foliar application of selenium nanoparticles (SeNPs) has been used to enhance Se concentration in winter wheat, but soil application of SeNPs on Se uptake in the crop and their transformation in soil are still limited. This study investigated the effects of varying sizes (50, 100, 200 nm) and concentrations (0, 2, 5, 25, 100 mg kg-1) of chemical synthesized SeNPs in soil on uptake and accumulation of Se in the crop at maturity and related mechanisms. SeNPs not only posed very low toxic to plant growth, except for leaf, but also significantly enhanced grain Se concentration. Regardless of concentration of SeNPs added to soil, the transformation rate of the larger sized SeNPs (200 nm) in soil was significantly (p < 0.05) higher than that of the smaller one, which is mainly due to the latter was more easily adsorbed onto soil organic matter and reluctant to be oxidized. Significantly higher grain Se concentration under the larger sized SeNPs contributed to significantly higher transformation rate of SeNPs and concentration of available Se in soil. The present study showed that the larger sized SeNPs in soil had significant advantages including higher grain Se concentration and Se utilization efficiency for wheat Se biofortification.

Protecting water birds of wetlands: Using toxicological tests and ecological risk assessment, based on metal/loid (s) of water, sediment and biota samples.

Water, sediment and biota (two fish and one shrimp species) samples were collected from rivers, ponds, mudflat and estuaries within the Mai Po Ramsar site in Hong Kong, China, to determine the concentrations of heavy metals/loid (HMs): cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb), zinc (Zn), and arsenic (As). The mutagenicity and toxicity of water and sediment samples were evaluated using Ames test and Microtox® bioassay, respectively. Ecological risk assessments on water birds were also conducted based on HM concentrations in biota, sediment and water samples. In general, results showed that downstream of Shan Pui River and Ng Tung River had relatively higher HM concentrations (e.g. Cu: 0.992-9.49 µg/L; As: 0.342-5.83 µg/L) when compared with estuaries (Cu: 0.400-3.69 µg/L; As: 0.220-0.931 µg/L) and ponds (Cu: 0.0402-2.41 µg/L; As: 0.0501-2.10 µg/L), except Hg and Cd. On the other hand, sediment samples collected from Ng Tung River showed mutagenicity on Salmonella typhimurium tester strain TA100, while the mudflat samples had mutagenicity on tester strain TA98 used in the Ames tests. Microtox® bioassay indicated that only sediment of Ng Tung River showed acute toxicity with a 15 min half maximal effective concentration (EC50) value of 30.5%. Results of ecological risk assessments showed that grey heron (Ardea cinerea), which feeds on fish and shrimps at Shan Pui River and ponds were not at risk. However, common sandpiper (Actitis hypoleucos), which feeds at ponds were subjected to a potential health risk due to its behavior of seeking food from the sediments, especially this bird species has a small body size. This indicates juvenile birds of other species may also be affected. Remediation should be implemented to relief the potential risk of Cu and Pb, to these water birds in the Mai Po Ramsar site.

Comparison of ashing and pyrolysis treatment on cadmium/zinc hyperaccumulator plant: Effects on bioavailability and metal speciation in solid residues and risk assessment.

Phytoremediation of metal(loid)s contaminated sites is widely used, while there is scarce of investigation on the metal-enriched biomass waste safely disposal which resulted in risks of causing secondary pollution to the soil and water bodies and even to human health. Thus, this study compared the effects of ashing and pyrolysis treatments on cadmium (Cd) and zinc (Zn) hyperaccumulation plant Sedum plumbizincicola. Chemical speciation, the Toxicity Characteristic Leaching Procedure (TCLP), and diethylenetriamine pentaacetic acid (DTPA) extraction were employed to characterize the bioavailability and leachability of Cd and Zn in the solid residues after pyrolysis and ashing. The risk assessment code (RAC) and potential ecological risk index (RI) were subsequently used to evaluate the risk of the solid residues to the environment. The results showed that both ashing and pyrolysis treatments could transform the bioavailable Cd and Zn in S. plumbizincicola into a more stable form, and the higher the temperature the greater the stablility. Pyrolysis converted a maximum of 80.0% of Cd and 70.3% of Zn in S. plumbizincicola to the oxidisable and residual fractions, compared with ashing which achieved only a ∼42% reduction. The pyrolysis process minimised the risk level of Cd and Zn to the environment based on the RAC and RI assessments. The results of the TCLP test, and DTPA extraction confirmed that the leaching rate and the bioavailable portion of Cd and Zn in the biochars produced by pyrolysis were invariably significantly (p < 0.05) lower than the solid residues produced by ashing, and reached the lowest at 650 °C. In other words, pyrolysis was better than ashing for thermal treatment of the metal-enriched hyperaccumulator plant, in view of minimising the bioavailability and leachability of Cd and Zn from the solid residues to the environment. This study provides fundamental data on the choice of treatments for the disposal of metal-enriched plant biomass.