RESUMEN
INTRODUCTION: The aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures. METHODS: Random amplification of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, State of Mato Grosso do Sul, Brazil, from 1998-2000. RESULTS: The most frequently isolated species was Candida albicans (45.8%). DNA amplification from genomic yeast isolates indicated a genetic similarity of over 90%. CONCLUSIONS: The RAPD profiles obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.
Asunto(s)
Candidiasis/microbiología , Fungemia/microbiología , Brasil/epidemiología , Candida/clasificación , Candida/genética , Candida/aislamiento & purificación , Candidiasis/epidemiología , ADN de Hongos/genética , Electroforesis en Gel de Agar , Fungemia/epidemiología , Humanos , Técnicas de Tipificación Micológica , Técnica del ADN Polimorfo Amplificado Aleatorio , Estudios RetrospectivosRESUMEN
The study involved 100 yeast isolates, obtained from urine samples provided by a Public Pediatric Hospital of São Paulo, Brazil, from 1999 to 2004. The most frequent species was Candida albicans, followed by C. tropicalis, C. glabrata and C. parapsilosis. In regard to virulence, 97% of the isolates showed index 3 for proteinase and 63% index 2 for phospholipase. The most frequent killer biotypes were 511 and 888.
Asunto(s)
Candida/clasificación , Candidiasis/orina , Infecciones Urinarias/microbiología , Brasil , Candida/enzimología , Candida/aislamiento & purificación , Candidiasis/microbiología , Niño , Preescolar , Humanos , Lactante , Recién Nacido , Péptido Hidrolasas/metabolismo , Fosfolipasas/metabolismoRESUMEN
The study involved 100 yeast isolates, obtained from urine samples provided by a Public Pediatric Hospital of São Paulo, Brazil, from 1999 to 2004. The most frequent species was Candida albicans, followed by C. tropicalis, C. glabrata and C. parapsilosis. In regard to virulence, 97 percent of the isolates showed index 3 for proteinase and 63 percent index 2 for phospholipase. The most frequent killer biotypes were 511 and 888.
Estudou-se 100 amostras de leveduras, isoladas de urina, provenientes de Hospital Público Infantil de São Paulo Brasil, no período de 1999-2004. A espécie mais freqüente foi Candida albicans, seguida de C. tropicalis, C. glabrata e C. parapsilosis. Em relação à virulência, 97 por cento dos isolados apresentaram índice 3 para proteinase e, 63 por cento índice 2 para fosfolipase. Os biótipos "killer" mais freqüentes foram o 511 e 888.
