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1.
PLoS One ; 19(4): e0299825, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38593174

RESUMEN

Chilling sensitivity is one of the greatest challenges affecting the marketability and profitability of sweet basil (Ocimum basilicum L.) in the US and worldwide. Currently, there are no sweet basils commercially available with significant chilling tolerance and traditional aroma profiles. This study was conducted to identify quantitative trait loci (QTLs) responsible for chilling tolerance and aroma compounds in a biparental mapping population, including the Rutgers advanced breeding line that served as a chilling tolerant parent, 'CB15', the chilling sensitive parent, 'Rutgers Obsession DMR' and 200 F2 individuals. Chilling tolerance was assessed by percent necrosis using machine learning and aroma profiling was evaluated using gas chromatography (GC) mass spectrometry (MS). Single nucleotide polymorphism (SNP) markers were generated from genomic sequences derived from double digestion restriction-site associated DNA sequencing (ddRADseq) and converted to genotype data using a reference genome alignment. A genetic linkage map was constructed and five statistically significant QTLs were identified in response to chilling temperatures with possible interactions between QTLs. The QTL on LG24 (qCH24) demonstrated the largest effect for chilling response and was significant in all three replicates. No QTLs were identified for linalool, as the population did not segregate sufficiently to detect this trait. Two significant QTLs were identified for estragole (also known as methyl chavicol) with only qEST1 on LG1 being significant in the multiple-QTL model (MQM). QEUC26 was identified as a significant QTL for eucalyptol (also known as 1,8-cineole) on LG26. These QTLs may represent key mechanisms for chilling tolerance and aroma in basil, providing critical knowledge for future investigation of these phenotypic traits and molecular breeding.


Asunto(s)
Ocimum basilicum , Sitios de Carácter Cuantitativo , Humanos , Ocimum basilicum/genética , Fitomejoramiento , Mapeo Cromosómico/métodos , Fenotipo , Genómica , Polimorfismo de Nucleótido Simple , Ligamiento Genético
2.
PhytoKeys ; (55): 93-111, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26312045

RESUMEN

Big-bracted dogwoods (Cornus sp.) are well-known plants in North America and eastern Asia where they occur as wild, generally spring-flowering understory trees. They are also popular ornamental landscape plants, and many economically important cultivars are propagated and sold across North America, Europe, and Asia. Starting in the late 1960s, Elwin Orton of Rutgers University in New Jersey (USA) utilized three geographically disjunct species of dogwoods, Cornusflorida (eastern North America), Cornusnuttallii (western North America), and Cornuskousa (East Asia), in an extensive interspecific hybridization program. He was successful in developing the first-ever interspecific F1 hybrids of these species, several of which have become staple items in the ornamental nursery trade due to their enhanced ornamental qualities and resistance to diseases. The original F1 plants are still alive at Rutgers University. While they have been available for decades in horticultural commerce, the interspecific hybrid crosses were never formally described and their scientific hybrid names were never published. For the Cornuskousa × Cornusflorida hybrids, the name Cornus 'rutgersensis' has been used on occasion in the horticultural trade, but without proper citation and description. Here, it is formally named Cornus×rutgersensis Mattera, T. Molnar, & Struwe, hybr. nov. For the Cornuskousa × Cornusnuttallii hybrids, no previous name has been used, and it is hereby named Cornus×elwinortonii Mattera, T. Molnar, & Struwe, hybr. nov. The need for providing scientific names for commonly used horticultural hybrids is discussed. Holotype material for both hybrid names was collected from the original F1 hybrids for full documentation, typification, and description. The comparative intermediate development of leaves, inflorescence structures, and fruit types of the hybrids and their parents is discussed and illustrated. Etymology, phenology, and cultivation aspects of these hybrids and their cultivars including backcrosses to Cornuskousa are also presented.

3.
AoB Plants ; 72015 Jan 06.
Artículo en Inglés | MEDLINE | ID: mdl-25564515

RESUMEN

Plants require nitrogen (N) to make proteins, nucleic acids and other biological molecules. It is widely accepted that plants absorb inorganic forms of N to fill their needs. However, recently it has become clear that plants also have the capacity to absorb organic N from soils. In this paper we describe a new kind of symbiosis involving seed-vectored rhizobacteria and grasses that is targeted at enhancing acquisition of organic N from soils. Our proposal is based on results of experiments on seedlings of grass species Festuca arundinacea Schreb., Lolium perenne L. and Poa annua L. that suggest: (i) seed-vectored rhizobacteria colonize seedling roots and influence their development; (ii) reactive oxygen secretion by seedling roots plays a role in organic N procurement by denaturing microbial proteins in the vicinity of roots (daytime activity); and (iii) plant root and microbial proteases degrade denatured proteins prior to absorption by roots (night-time activity). This research involved the following types of studies: (i) seedling root development experiments with and without rhizobacteria on a variety of substrates in agarose media and (ii) isotopic N-tracking experiments to evaluate the absorption into seedlings of N obtained from degradation of proteins. We hypothesize that grasses, in particular, are adapted to scavenge organic N from soils through application of this 'oxidative nitrogen scavenging' symbiosis with rhizobacteria, and their soil-permeating root systems. This newly discovered symbiosis in grass species could lead to new ways to cultivate and manage grasses to enhance efficiency of N utilization and reduce applications of inorganic fertilizers.

4.
Symbiosis ; 57(3): 161-171, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23087539

RESUMEN

In this paper we propose and provide evidence for a mechanism, oxidative nitrogen scavenging (ONS), whereby seedlings of some grass species may extract nitrogen from symbiotic diazotrophic bacteria through oxidation by plant-secreted reactive oxygen species (ROS). Experiments on this proposed mechanism employ tall fescue (Festuca arundinaceae) seedlings to elucidate features of the oxidative mechanism. We employed 15N(2) gas assimilation experiments to demonstrate nitrogen fixation, direct microscopic visualization of bacteria on seedling surfaces to visualize the bacterial oxidation process, reactive oxygen probes to test for the presence of H(2)O(2) and cultural experiments to assess conditions under which H(2)O(2) is secreted by seedlings. We also made surveys of the seedlings of several grass species to assess the distribution of the phenomenon of microbial oxidation in the Poaceae. Key elements of the proposed mechanism for nitrogen acquisition in seedlings include: 1) diazotrophic bacteria are vectored on or within seeds; 2) at seed germination bacteria colonize seedling roots and shoots; 3) seedling tissues secrete ROS onto bacteria; 4) bacterial cell walls, membranes, nucleic acids, proteins and other biological molecules are oxidized; 5) nitrates and/or smaller fragments of organic nitrogen-containing molecules resulting from oxidation may be absorbed by seedling tissues and larger peptide fragments may be further processed by secreted or cell wall plant proteases until they are small enough for transport into cells. Hydrogen peroxide secretion from seedling roots and bacterial oxidation was observed in several species in subfamily Pooideae where seeds possessed adherent paleas and lemmas, but was not seen in grasses that lacked this feature or long-cultivated crop species.

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