RESUMEN
This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.
Asunto(s)
Péptidos Catiónicos Antimicrobianos/farmacología , Lipopolisacáridos/antagonistas & inhibidores , Familia de Multigenes/efectos de los fármacos , Porphyromonas/efectos de los fármacos , Prevotella/efectos de los fármacos , Catelicidinas , Humanos , Porphyromonas/clasificación , Porphyromonas gingivalis/efectos de los fármacos , Prevotella intermedia/efectos de los fármacos , Prevotella melaninogenica/efectos de los fármacosAsunto(s)
Péptidos Catiónicos Antimicrobianos/farmacología , Síndrome de Behçet/microbiología , Lipopolisacáridos/farmacología , Streptococcus sanguis/efectos de los fármacos , Antiinfecciosos/farmacología , Catelicidinas , Humanos , Pruebas de Sensibilidad Microbiana , Streptococcus/efectos de los fármacosAsunto(s)
Virus de la Enfermedad Infecciosa de la Bolsa/fisiología , Monocitos/microbiología , Animales , Línea Celular , Células Cultivadas , Embrión de Pollo , Pollos , Efecto Citopatogénico Viral , Fibroblastos/microbiología , Virus de la Enfermedad Infecciosa de la Bolsa/ultraestructura , Leucemia Mieloide/veterinaria , Microscopía Electrónica , Organismos Libres de Patógenos Específicos , Células Tumorales CultivadasRESUMEN
We examined the morphological changes in gastric mucosa and the generation of ammonia after exposure of the rat stomach to urea in the presence of urease, in attempts to investigate a pathophysiological role of urea, urease, and ammonia system in gastric ulcer diseases. Exposure of the stomach for 20 min to 2 ml urea (0.025-0.2%) together with urease (100 IU) induced histological damages in a concentration-related manner. Either urea or urease alone did not induce any histological change in the mucosa. Instillation of urea into the stomach generated ammonia in the presence of urease; the amount of ammonia was increased depending on the concentration of urea, and was closely associated with the severity of histological damage. The exposure of the stomach to ammonia (NH4OH: 0.01-0.1%) also produced histological damages in the gastric mucosa in a concentration-related manner. The characteristics of injury induced by 0.5-1.0% ammonia were stasis of microcirculation, disruption of the surface epithelial cells, and necrosis of the mucosa. These results demonstrated that ammonia generated from the hydrolysis of urea by urease in the stomach causes damages in the gastric mucosa.
Asunto(s)
Amoníaco/metabolismo , Mucosa Gástrica/patología , Úlcera Gástrica/etiología , Urea/metabolismo , Ureasa/metabolismo , Amoníaco/efectos adversos , Animales , Mucosa Gástrica/efectos de los fármacos , Helicobacter pylori/enzimología , Hidrólisis , Masculino , Ratas , Ratas EndogámicasRESUMEN
A 46-day-old infant weighing 2250 g with the coarctation of the aorta, ventricular septal defect, PDA, the anomalous right subclavian artery, and the persistent left superior vena cava initially underwent by the reversed subclavian flap aortoplasty with the anomalous right subclavian artery and pulmonary arterial banding. The reversed subclavian flap aortoplasty was useful for the coarctation complex with the anomalous right subclavian artery. Two months later he gained weight to 2500 g, and then VSD closure and debanding of PAB was performed successfully. This two-staged operation was recommended for the poor risk coarctation complex with the anomalous subclavian artery.
