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Int J Hematol ; 77(5): 476-81, 2003 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12841386

RESUMEN

Coexistence of Philadelphia chromosome-negative (Ph-) progenitors with the Ph+ clone in the early chronic phase of chronic myeloid leukemia (CML) has been documented in previous reports. A different evaluation of methods is needed to justify the clonality of the residual Ph- progenitors. Therefore, the X chromosome inactivation patterns in individual granulocyte-monocyte colony-forming unit (CFU-GM) colonies were studied with the clonality assay for the human androgen receptor gene. A prerequisite for this evaluation was the validation of T-lymphocytes and buccal cells as control cells representing the constitutional lyonization. The percentages of polyclonal CFU-GM cells were determined in 9 Ph+ women with CML and in 5 healthy women. Results of the clonal analysis of CFU-GM colonies were compared with those from reverse transcriptase-polymerase chain reaction analysis of single colonies for BCR/ABL transcripts. Both methods of CFU-GM cell analysis were in agreement regarding the presence of variable proportions (0%-94%) of normal cells in CML. Our results suggest that (a) T-cells and buccal cells have potential for use as controls for the clonal analysis of CML cases and (b) this method can evaluate the frequency of polyclonal/clonal CFU-GM cells in CML cases and is applicable to the analysis of myeloid clonal disorders that lack specific molecular markers.


Asunto(s)
Leucemia Mielógena Crónica BCR-ABL Positiva/patología , Células Progenitoras Mieloides/patología , Receptores Androgénicos/genética , Adulto , Estudios de Casos y Controles , Células Clonales/patología , Compensación de Dosificación (Genética) , Femenino , Proteínas de Fusión bcr-abl/genética , Humanos , Leucemia Mieloide Crónica Atípica BCR-ABL Negativa/patología , Métodos , Persona de Mediana Edad , ARN Mensajero/análisis
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