The hidden majority/minority consensus: Minorities show similar preference patterns of immigrant support as the majority population.

The acceptance of new arrivals has become an important topic regarding the social cohesion of the receiving countries. However, previous studies focused only on the native population's drivers of attitudes towards immigrants, disregarding that immigrant-origin inhabitants now form a considerable part of the population. To test whether the drivers for the willingness to support immigrants are the same for natives and immigrants and their descendants, we rely on a vignette study conducted in a representative German online panel (N = 3149) which contains an overrepresentation of immigrant-origin respondents. We presented participants with three vignettes of potential immigrants, varying, amongst other factors, economic prospects, safe and war-ridden countries of origin (to capture deservingness), as well as religious identity. While we find that minority members are generally slightly more welcoming towards immigrants than majority members, at their core are the same factors that drive attitudes to immigrants in both groups: economic cost, cultural similarity, and deservingness. However, we observe differences at the margins: Immigrant-origin respondents take into account economic prospects to a lesser degree than majority members do, and by trend, they are less likely to distinguish between immigrants from war-ridden and safe countries of origin. Furthermore, we can show that the preference for immigrants with the same religious identities not only occurs among majority members but also among minority members.

Prediction of inclusion body solubilization from shaken to stirred reactors.

Inclusion bodies (IBs) were solubilized in a µ-scale system using shaking microtiter plates or a stirred tank reactor in a laboratory setting. Characteristic dimensionless numbers for mixing, the Phase number Ph and Reynolds number Re did not correlate with the kinetics and equilibrium of protein solubilization. The solubilization kinetics was independent of the mixing system, stirring or shaking rate, shaking diameter, and energy input. Good agreement was observed between the solubilization kinetics and yield on the µ-scale and laboratory setting. We show that the IB solubilization process is controlled predominantly by pore diffusion. Thus, for the process it is sufficient to keep the IBs homogeneously suspended, and additional power input will not improve the process. The high-throughput system developed on the µ-scale can predict solubilization in stirred reactors up to a factor of 500 and can therefore be used to determine optimal solubilization conditions on laboratory and industrial scale.

High-throughput system for determining dissolution kinetics of inclusion bodies.

Efficient solubilization is a crucial step during inclusion body processing and dissolving conditions were usually empirically established. Here we describe a new methodology for rapid screening of solubilization conditions and evaluation of dissolution kinetics in microtiter plates. Increase of protein in solution over time was directly related to decrease of turbidity measured by absorbance at 600 nm. Dissolution kinetics of inclusion bodies were described by a first-order reaction kinetics, which was used for drug dissolution modeling. Reaction constants were in the range of 0.01-0.03 s(-1) for buffer conditions providing sufficient solubilization power. This method is not limited to the screening of optimal buffer conditions for solubilization and can be applied for studying other parameters involved in the solubility of IBs, such as pI of the protein, influence of fermentation conditions, influence of initial protein concentration, and more.