1.
Lett Appl Microbiol
; 18(2): 117-119, 1994 Feb.
Artículo
en Inglés
| MEDLINE
| ID: mdl-34892925
RESUMEN
Commercially-available phenol red indicator, purified by adsorption chromatography was incorporated into lauryl sulphate broth (LSB) used in the membrane filtration method for the detection of Escherichia coli and other coliform bacteria. Relative to LSB containing the impure dye or its major contaminant, the purified phenol red provided clear visualization of discrete yellow colonies observed against a white background. The colonies remained stable for at least 24 h at 25°C under standard laboratory lighting conditions. This simple procedure will enhance the detection of coliforms in samples.