Continuous sound from a marine vibrator causes behavioural responses of free-ranging, spawning Atlantic cod (Gadus morhua).

Marine vibrators are a new technology being developed for seismic surveys. These devices can transmit continuous instead of impulsive sound and operate over a narrower frequency band and at lower peak pressure than airguns, which is assumed to reduce their environmental impacts. We exposed spawning Atlantic cod (Gadus morhua) to sound produced by a prototype, but full-scale, marine vibrator, and monitored behavioural responses of tagged cod using acoustic telemetry. Fish were exposed to 10 × 3 h continuous sound treatments over a 4-day period using a randomised-block design. Sound exposure levels were comparable to airgun exposure experiments conducted previously with the same set-up ranging from ∼115 to 145 dB re 1 µPa2s during exposure. Telemetry data were used to assess 1) whether marine vibrator exposure displaced cod from the spawning ground, through estimation of residence and survival probabilities, and 2) fine-scale behavioural responses within the test site, namely swimming depth, activity levels, displacement, and home ranges. Forty-two spawning cod were tagged prior to the exposure, with 22 present during the exposure. All 22 tags were equipped with pressure sensors and ten of these additionally with accelerometers. While no premature departure from the spawning site was observed, cod reacted to the exposure by decreasing their activity levels (by up to 50%, SE = 7%) and increasing their swimming depth (by up to 2.5 m, SE = 1.0 m) within the test site during the exposure period. These behavioural responses varied by sex and time of day. Cod reactions to a marine vibrator may be more pronounced than reactions to airguns, possibly because continuous sound is more disturbing to fish than intermittent sound at the same exposure levels. However, given sample size limitations of the present study, further studies with continuous sound are necessary to fully understand its impact and biological significance.

Antarctic sea-ice low resonates in the ecophysiology of humpback whales.

The past six years have been marked by some of the most dramatic climatic events observed in the Antarctic region in recent history, commencing with the 2017 sea-ice extreme low. The Humpback Whale Sentinel Programme is a circum-polar biomonitoring program for long term surveillance of the Antarctic sea-ice ecosystem. It has previously signalled the extreme La Niña event of 2010/11, and it was therefore of interest to assess the capacity of existing biomonitoring measures under the program to detect the impacts of 2017 anomalous climatic events. Six ecophysiological markers of population adiposity, diet, and fecundity were targeted, as well as calf and juvenile mortality via stranding records. All indicators, with the exception of bulk stable isotope dietary tracers, indicated a negative trend in 2017, whilst C and N bulk stable isotopes appeared to indicate a lag phase resulting from the anomalous year. The collation of multiple biochemical, chemical, and observational lines of evidence via a single biomonitoring platform provides comprehensive information for evidence-led policy in the Antarctic and Southern Ocean region.

Use of unmanned aerial vehicles (UAVs) for mark-resight nesting population estimation of adult female green sea turtles at Raine Island.

Nester abundance is a key measure of the performance of the world's largest green turtle rookery at Raine Island, Australia, and has been estimated by mark-resight counts since 1984. Nesters are first marked by painting their carapace with a longitudinal white stripe. Painted and unpainted turtles are then counted by a surface observer on a small boat in waters adjacent to the reef. Unmanned aerial vehicles (UAV) and underwater video may provide more cost-effective and less biased alternatives to this approach, but estimates must be comparable with historical estimates. Here we compare and evaluate the three methods. We found comparatively little variation in resighting probabilities between consecutive days of sampling or time of day, which supports an underlying assumption of the method (i.e. demographic closure during sampling). This lack of bias in the location availability for detection of painted versus unpainted turtles and further supported by a parallel satellite tracking study of 40 turtles at Raine Island. Our results demonstrated that surface observers consistently reported higher proportions of marked turtles than either the UAV or underwater video method. This in turn yielded higher population estimates with UAV or underwater video compared to the historical surface observer method, which suggested correction factors of 1.53 and 1.73 respectively. We attributed this to observer search error because a white marked turtle is easier to spot than the non-marked turtle. In contrast, the UAV and underwater video methods allowed subsequent frame-by-frame review, thus reducing observer search error. UAVs were the most efficient in terms of survey time, personnel commitment and weather tolerance compared to the other methods. However, underwater video may also be a useful alternative for in-water mark-resight surveys of turtles.

Green turtle (Chelonia mydas) population demographics at three chemically distinct foraging areas in the northern Great Barrier Reef.

The catchments of the Great Barrier Reef (GBR) have experienced significant modifications in recent decades, leading to increases in sources of pollutants and declines in coastal water quality. As coastal waters of the GBR support some of the highest density green turtle (Chelonia mydas) foraging populations in the western Pacific Ocean, understanding the effects of contaminants on GBR green turtle populations is a priority. In 2012, elevated strandings of green turtles in the Upstart Bay region instigated the WWF's collaborative Rivers to Reef to Turtles (RRT) project to investigate if coastal pollutants are compromising green turtle health. Important to interpreting these investigations into toxicology and health is understanding the demographics of the green turtle populations being investigated. In three green turtle foraging grounds, Cleveland Bay (CLV), Upstart Bay (UPB) and the Howick Group of Reefs (HWK), this study explored population size, age class structure, sex ratio, growth rates, body condition and diet, as well as indices of turtle health, such as plastron barnacle loads and eye lesions. The three foraging populations had similar age class structure and adult sex ratios to other green turtle foraging populations in the GBR. Somatic growth rate was nonlinear, peaking in immature turtles, and was much slower in turtles foraging at HWK compared to the other two sites. This may have been due to differences in food source, which was supported by the observed dietary shifts between seagrass and algae in HWK turtles, compared to a consistently seagrass diet in CLV and UPB turtles. There were also small differences in body condition between sites, as well as differences in barnacle loads, eye lesions and occurrence of fibropapilloma tumors. This study provides important information on green turtle foraging ground population dynamics in the northern GBR, and context for the other papers in this special issue.

Screening of organic and metal contaminants in Australian humpback dolphins (Sousa sahulensis) inhabiting an urbanised embayment.

As a marine mammal species that inhabits shallow nearshore waters, humpback dolphins are likely exposed to a wide range of pollutants from adjacent land-based activities. Increased mortality rates of Australian humpback dolphins (Sousa sahulensis) in waters off a major urbanised centre triggered investigations into the threats to these species, including their contaminant exposure. The present study utilised archived tissues from 6 stranded animals to screen for a range of pollutants (PCDD/Fs, PBDEs, PCBs, organochlorine pesticides, PAHs, organotins, essential and non-essential elements) to inform future biopsy based biomonitoring strategies. Concentrations of PCBs and DDXs in blubber of some of these animals were remarkably high, at levels near or above toxicological thresholds associated with immune- and reproductive toxicity or population declines in other marine mammals. PBDEs, PAHs, HCB, organotins, 'drins' as well as other organic pesticides were not detected, or present at relatively low concentrations. Profiles of elements were similar in epidermis compared to other tissues, and apart from some exceptions (e.g. Fe, Cr, Co, Cu) their concentrations fell within 25th-75th percentiles of cetacean baselines in four of the five animals. Non-essential elements (Al, V, Pb, Ba, Ni, Cd) were notably elevated in one specimen which may have experienced poor health or nutritional status. These data provide a first insight into the contaminant status of a rare and poorly studied population inhabiting an urbanised area. The results highlight a need for future biomonitoring of live populations, and inform on priorities in the typically limited blubber and skin sample volumes obtained through biopsies.

Mortality of inshore marine mammals in eastern Australia is predicted by freshwater discharge and air temperature.

Understanding environmental and climatic drivers of natural mortality of marine mammals is critical for managing populations effectively and for predicting responses to climate change. Here we use a 17-year dataset to demonstrate a clear relationship between environmental forcing and natural mortality of inshore marine mammals across a subtropical-tropical coastline spanning a latitudinal gradient of 13° (>2000 km of coastline). Peak mortality of inshore dolphins and dugongs followed sustained periods of elevated freshwater discharge (9 months) and low air temperature (3 months). At a regional scale, these results translated into a strong relationship between annual mortality and an index of El Niño-Southern Oscillation. The number of cyclones crossing the coastline had a comparatively weak effect on inshore marine mammal mortality, and only in the tropics. Natural mortality of offshore/migratory cetaceans was not predicted by freshwater discharge, but was related to lagged air temperature. These results represent the first quantitative link between environmental forcing and marine mammal mortality in the tropics, and form the basis of a predictive tool for managers to prepare responses to periods of elevated marine mammal mortality.

Multidimensionality of behavioural phenotypes in Atlantic cod, Gadus morhua.

Much of the inter-individual variation observed in animal behaviour is now attributed to the existence of behavioural phenotypes or animal personalities. Such phenotypes may be fundamental to fisheries and aquaculture, yet there have been few detailed studies of this phenomenon in exploited marine animals. We investigated the behavioural and neuroendocrine responses of Atlantic cod (Gadus morhua L.), to situations reflecting critical ecological challenges: predator attacks and territorial challenges. Both hatchery-reared and wild fish were tested and behavioural profiles were compared with baseline conditions. We then used an objective, multivariate approach, rather than assigning individuals along one-dimensional behavioural axes, to examine whether distinct behavioural phenotypes were present. Our results indicate that two distinct behavioural phenotypes were evident in fish from each background. In hatchery-reared fish, phenotypes displayed divergent locomotor activity, sheltering, brain monoamine concentrations and responses to competitive challenges. In wild fish, phenotypes were distinguished primarily by locomotor activity, sheltering and responsiveness to predator stimuli. Hatcheries presumably represent a more stressful social environment, and social behaviour and neuroendocrine responses were important in discerning behavioural phenotypes in hatchery fish, whereas antipredator responses were important in discerning phenotypes in wild fish that have previously encountered predators. In both fish types, behavioural and physiological traits that classified individuals into phenotypes were not the same as those that were correlated across situations. These results highlight the multidimensionality of animal personalities, and that the processes that regulate one suite of behavioural traits may be very different to the processes that regulate other behaviours.

Effects of turbidity on the spontaneous and prey-searching activity of juvenile Atlantic cod (Gadus morhua).

Increasing turbidity in coastal waters in the North Atlantic and adjacent seas has raised concerns about impacts on Atlantic cod (Gadus morhua) using these areas as nurseries. A previous experiment (Meager et al. 2005 Can. J. Fish. Aquat. Sci. 62, 1978-1984) has shown that turbidity (up to 28 beam attenuation m-1) had little effect on the foraging rate of juvenile cod. Although this was attributed to cod using chemoreception in conjunction with vision to locate prey, foraging rates may also be maintained by increased activity. Higher activity, however, is energetically costly and may offset benefits from increased foraging return. We examined the effects of turbidity on prey searching and spontaneous activity of juvenile cod in the laboratory, by measuring activity with and without prey cues. Activity of juvenile cod was nonlinearly affected by turbidity and was lower at intermediate turbidity, regardless of the presence of prey odour. Activity increased over time when prey odour was present and decreased when absent, but the effects of prey odour were similar across all turbidity levels. Position in the tank was unaffected by turbidity or prey odour. Reduced activity at intermediate turbidities is likely to offset longer prey-search times. At high turbidity (greater than 17m-1), both longer prey-search times and higher activity indicate that increased energetic costs are probable.

Escape responses in juvenile Atlantic cod Gadus morhua L.: the effects of turbidity and predator speed.

We examined the effect of turbidity (0.5-14 beam attenuation m(-1)) and predator attack speed (150 and 296 cm s(-1)) on escape responses of juvenile cod Gadus morhua in the laboratory. We triggered escape responses using a predator model and measured escape timing, direction and locomotor performance. We also measured responsiveness and estimated the likelihood of fish escaping the ;predator attack' (putative escape success, PES). Turbidity affected both PES and the type of escape response used by the fish, but these effects depended on predator speed. PES for the fast predator attack declined from 73% in clear water to 21% in highly turbid water, due to decreased responsiveness and poorly timed escapes. Intermediate turbidity enhanced PES and responsiveness to the slow predator attack. Locomotor performance was reduced by turbidity, whereas predator speed had the opposite effect. Our results suggest that both predator attack speed and turbidity have important roles in determining the vulnerability of fish attacked by piscivorous predators.