Fat globule diameter in infant formulas.

OBJECTIVES: The lipid fraction, fatty acid profile, and diameter of fat globules of infant formulas show great differences from human milk. These characteristics influence fat digestion and, consequently, the development and health of infants. The aim of this study was to evaluate the diameter of fat globules in infant formulas and compare them with those in human milk. METHODS: The diameter of fat globules of 10 infant formulas and human milk samples was determined using scanning electron microscopy. RESULTS: The starter infant formula was the only one that showed a mean diameter value (3.52 ± 2.17 µm) similar to that obtained for human milk (3.44 ± 1.68 µm). The starter infant formula showed the highest values of volume-surface D3,2 (6.13 µm) and volume-weighted D4,3, (7.05 µm) mean diameters among the infant formulas analyzed, and close to those obtained for the human milk sample (5.16 and 5.98 µm, respectively). The infant formulas whey protein partially hydrolyzed, soy protein isolate-based, whey protein extensively hydrolyzed, and thickened with pregelatinized starch had the lowest mean diameters of fat globules 0.64 ± 0.22, 0.70 ± 0.19, 1.06 ± 0.34, and 1.22 ± 0.48 µm, respectively. CONCLUSION: The analysis of principal components showed that none of the analyzed infant formulas had similarity with the diameter of fat globules and the fatty acid profile of human milk.

Ozonation of Brazil nuts (Bertholletia excelsa H.B.K. ): effect of column height on the saturation process and changes in quality / Ozonização de castanha-do-Brasil (Bertholletia excelsa H.B.K. ): efeito da altura da coluna no processo de saturação e alterações na qualidade

The objective of this study is to examine the saturation process in a column containing Brazil nuts and possible changes in the quality of the product. Brazil nut samples were initially placed in a cylindrical PVC column 15 cm in diameter and 110 cm in height. The ozone gas concentrations of 2.5, 4.5, 9.0, and 14.0 mg L-1 and a flow rate of 3.0 L min-1 were applied at a temperature of 25 ºC. Ozone gas was injected at the base of the cylindrical column, and the seed column height values adopted were 0.25, 0.50, and 0.75 m. Saturation concentration and time were determined. To measure possible changes in the quality of ozonized Brazil nuts, moisture and color, as well as qualitative variables of the crude oil were evaluated at the exposure times of 0, 3, 6, 9, and 12 h. To evaluate the quality of the crude oil extracted from ozonized nuts, the free fatty acid content, peroxide value, and iodine value were analyzed. Increasing ozonation times increased ozone concentration at all inlet gas concentrations. Saturation time decreased as the inlet gas concentration was increased, at the different product column heights. There was no change in product moisture in response to ozonation. Ozonation did not induce significant changes in color or in the crude oil, due to the triple interaction between column height, ozone concentration, and exposure time. In conclusion, the height of the product's column influences saturation time and concentration during the ozonation process. Considering the color of the product and characteristics of its crude oil, the use of ozone under the conditions adopted in the present study does not affect the quality of Brazil nuts to the point of rendering them unmarketable.

O objetivo do presente trabalho é estudar o processo de saturação em coluna contendo castanha-do-Brasil e possíveis alterações na qualidade do produto. Inicialmente as amostras de castanha-do-Brasil foram acondicionadas em coluna cilíndrica de PVC de 15 cm de diâmetro e 110 cm de altura. Foram adotadas as concentrações do gás ozônio de 2,5, 4,5, 9,0 e 14,0 mg L-1 e vazão de 3,0 L min-1, na temperatura de 25 ºC. O gás ozônio foi injetado na base da coluna cilíndrica e os valores adotados de altura da coluna de grãos foram de 0,25, 0,50, e 0,75 m. Determinaram-se o tempo e a concentração de saturação. Na avaliação de possíveis alterações na qualidade de castanhas-do-Brasil ozonizadas foram determinados a umidade, coloração e variáveis qualitativas do óleo bruto, com tempos de exposição de 0, 3, 6, 9 e 12 h. Para avaliação da qualidade do óleo bruto extraído de castanhas ozonizadas foram analisadas o teor de ácidos graxos livres, o índice de peróxido e o índice de iodo. A elevação do período de ozonização promoveu aumento da concentração do ozônio para todas as concentrações de entrada do gás. No que se refere aos valores de tempo de saturação, à medida que se elevou a concentração de entrada do gás, houve redução do tempo de saturação, nas diferentes alturas de coluna do produto. Não houve variação da umidade do produto em decorrência da ozonização. A ozonização não provocou alterações significativas na cor e no óleo bruto, em decorrência da interação tripla entre altura da coluna do produto, concentração do ozônio e tempo de exposição. É possível concluir que a altura da coluna do produto influencia o tempo e a concentração de saturação, durante o processo de ozonização. O uso do ozônio nas condições adotadas no presente estudo não afeta a qualidade da castanhado-Brasil, considerando-se a cor do produto e características do óleo bruto, de tal forma a inviabilizar a comercialização.

Influence of Different Cooking Methods on Fillet Steak Physicochemical Characteristics.

Meat is a source of protein widely consumed by the population in many countries due mainly to the nutritional aspects, sensory characteristics, and cultural aspects. The meat cooking preparation can promote significant changes in the meat's chemical composition and physical characteristics. Such transformations can impact both the acceptance of the product and consumers' health. Due to the different thermal processes altering the physical-chemical characteristics of meat, the present study aimed to evaluate the physicochemical characteristics of fillet steak submitted to different cooking methods: pan-frying with and without oil and air fryer. We performed the analysis to evaluate the physicochemical characteristics considering moisture, lipid, protein, ash, sodium, and potassium content, cooking loss index and colorimetry in three degrees of doneness of the meat, rare, medium, and well done. The fillet steak prepared by pan-frying with oil lost higher moisture and weight than the other samples. The air fryer method presented the highest moisture content. There was a significant difference in lipid content in which the pan-frying with oil fillet steak showed the highest amount of lipids. The pan-frying with oil steak fillet also presented more changes in the colorimetric parameters evaluated compared to the other samples. The pan-frying with oil cooking method promoted more pronounced changes in the steak fillet, and the cooking air fryer, the changes in meat quality are less pronounced. Therefore, the air fryer can be considered a good alternative for cooking meat.

Impact of Gluten-Free Sorghum Bread Genotypes on Glycemic and Antioxidant Responses in Healthy Adults.

Sorghum is used to provide good quality gluten-free products due to phytochemicals and low glycemic index (GI). This study aimed to determine the chemical composition, the antioxidant activity and capacity, and the glycemic and insulinemic responses of gluten-free (GF) sorghum bread. GF bread samples were produced with three different sorghum genotypes. The samples were analyzed for chemical composition, resistant starch and dietary fiber content; antioxidant activity by ORAC; antioxidant capacity by FRAP; GI; and insulinemic responses. This double-blind, crossover, randomized clinical trial was conducted with 10 healthy men aged 28.0 ± 4.9 years (77.6 ± 11.7 kg and 24.2 ± 2.3 kg/m2). All sorghum bread showed significantly more fiber than rice bread (control). Brown sorghum bread was classified as low GI, bronze and white as medium GI, and control as high GI. Brown sorghum bread presented a low carbohydrate content, a significant amount of fiber, and a significantly lower 3 h AUC glucose response than those of the control, aside from the highest antioxidant activity value (p ≤ 0.001). Therefore, brown sorghum was superior to other genotypes analyzed in this study, and its production should be encouraged to provide gluten-free products with a better nutritional profile. More research is required to explore the effects of different sorghum genotypes in food products on human health.

Amorphophallus konjac: A Novel Alternative Flour on Gluten-Free Bread.

The demand for gluten-free products is rising, but their production with similar quality as their gluten counterparts is challenging. This study aimed to develop gluten-free bread samples using different concentrations of Amorphophallus konjac flour (0%, 12.5%, 25%, 37.5%, and 50% of the total flour content) and to evaluate their nutritional and physicochemical properties. Proteins, lipids, carbohydrates, moisture, ash content, fibers, resistant starch, firmness, specific volume, and color were evaluated using official methods. Protein varied from 2.95% to 4.94%, the energy value from 347.93 to 133.55 kcal/100 g, dietary fiber from 8.19 to 17.90%, and resistant starch from 0.67% to 0.75% on wet basis. The addition of konjac flour positively influenced the specific volume. Higher concentrations of konjac flour in the formulations led to lower calories of the bread due to the significant addition of water to the dough. The bread samples with konjac showed high fiber content due to the composition of the flour. They had lower levels of carbohydrates, which can positively influence the glycemic index. Konjac flour provided dough mold, growth, and better texture for gluten-free bread. The best formulations were prepared in concentrations up to 37.5% konjac. The 50% konjac bread showed slightly reduced specific volume and pale color.

Characterization of the kefir beverage produced from yam (Colocasia esculenta L.), sesame seed (Sesamum indicum L.) and bean (Phaseolus vulgaris L.) extracts.

The objective of this study was to evaluate the characteristics of kefir beverages made from yam, sesame and bean extracts after fermentation of the extracts with water kefir grains. Formulations were prepared with raw and boiled yams. To the yam extract, sesame and different percentages of white bean extract were added. The fermentation kinetics, the chemical composition and color of the extracts were analyzed. To determine the fermentation kinetic parameters, pH and titratable acidity were evaluated. The chemical composition and color of all extracts were determined before and after fermentation process. The decrease in pH and increase in titratable acidity were affected both by raw or boiler yam. The addition of bean extract favored the decrease in pH of formulations containing extracts of raw or boiled yams. Chemical composition and color of the beverages were altered after fermentation. The kefir beverage made from yam and sesame enriched with 50% beans proved to be an excellent fermentation substrate. In addition, it is a new way of consuming vegetable products, especially fermented products, which have limited diversity to serve vegan consumers and also people with certain allergies to dairy products.

Lipid profile of different infant formulas for infants.

Situations including premature infants, or those in which there is a rejection to breastfeeding, require the use infant formulas for total or partial replacement of human milk. The objective of this study was to determine the lipid content and to identify the lipid profile of infant formulas. Samples were collected from ten different infant formulas, used as a substitute for breast milk at the Maternal and Child Hospital of Brasilia. The human milk sample consisted of a pool of samples from 10 mature milk donors at the milk bank of the University Hospital of Brasilia. The lipid content and lipid profile of the different infant formulas and human milk were analyzed. The experiment was conducted in a randomized block design, with eleven treatments and three replicates, in triplicate. The data obtained in this study indicated significant differences between infant formulas and human milk, and among the infant formulas analyzed in relation to the percentage of total lipids and the fatty acid profile, except for the fractions of linoleic acid and linolenic acid. Regarding the percentage of polyunsaturated fatty acids in relation to the total unsaturated fatty acids, only the Soy Protein Isolate-based Infant Formula (SPIIF) and Whey Protein Extensively Hydrolyzed Infant Formula (WPEHIF) resembled human milk. It was concluded that despite the observed differences, the use of infant formulas is a viable strategy for the development of infants subjected or not to specific physiological conditions.

Are fat acids of human milk impacted by pasteurization and freezing?

The Human Milk Bank undergo human milk to pasteurization, followed by storage in a freezer at -18° C for up to six months to thus keep available the stocks of this product in maternal and infant hospitals. The objective of this study was to evaluate the effects of processing on the lipid fraction of human milk. A sample of human milk was obtained from a donor and was subdivided into ten sub-samples that was subjected to the following treatments: LC = raw milk; T0 = milk after pasteurization; T30 = milk after pasteurization and freezing for 30 days; T60 = milk after pasteurization and freeze for 60 days, and so on every 30 days until T240 = milk after pasteurization and freezing for 240 days, with 3 repetitions for each treatment. Lipids were extracted, methylated and fatty acid profiles determined by gas chromatography. The fatty acids were characterized by nuclear magnetic resonance and functional groups were identified by infrared spectroscopy. There were variations in the concentration of fatty acids. For unsaturated fatty acids there was increasing trend in their concentrations. The IR and NMR analyze characterized and identified functional groups presents in fatty acids.

Los Bancos de Leche Humana someten la leche la pasteurización, seguido del almacenamiento en un congelador a -18 grados por até seis meses, para así disponerlo a los Hospitales Materno Infantiles. El objetivo de este estudio fue evaluar los efectos del procesamiento de la fracción lipídica de la leche humana. Para esto, una muestra obtenida de una donante y sometida a diez diferentes tratamientos: LC = leche cruda; TO = leche después de la pasteurización; T30 = leche después de la pasteurización y congelación por 30 días; T60 = leche después de la pasteurización y congelación por 60 días y así sucesivamente a cada 30 días hasta T240 = leche después de la pasteurización y congelación por 240 días, con tres repeticiones a cada tratamiento. Los lípidos fueron extraídos y los ácidos grasos metilados fueron determinados por cromatografía gaseosa. Los ácidos grasos fueron caracterizados por resonancia magnética nuclear y los grupos funcionales identificados por espectroscopia infrarroja. Hubo variaciones en las concentraciones de ácidos grasos. Para los ácidos grasos insaturados hubo un aumento en sus concentraciones. Sin embargo, los grupos funcionales se caracterizaron por espectroscopia infrarroja y resonancia magnética nuclear, RMN.

Alterações físico-químicas em óleos de soja submetidos ao processo de fritura em unidades de produção de refeição no Distrito Federal / Physic-chemical alterations of soybean oils used in the frying process in food production units in the Brazilian Federal District

Objetivo: Analisar as alterações físico-químicas de óleos de soja, utilizados em processos de fritura intermitente, em unidades de produção de refeições em Brasília – Distrito Federal. Material e Métodos: O método utilizado foi descritivo com abordagem quantitativa. As análises foram coletadas de 02 em 02 dias, partindo-se da coleta do óleo após a frituras até seu descarte pelas unidades de produção de refeições. Incluiu o óleo virgem, com 03 repetições de cada amostra, totalizando 33 frações de amostras para análise de acidez titulável, índice de peróxido e índice de iodo. Os resultados foram analisados no programa Epi-info 2003. Palavras-chave: Óleo de fritura; Oxidação lipídica; Unidades de produção de refeição(AU). Objective: To evaluate the physic-chemical alteration of soybean oils used in the frying process in food production units (UPR), in Brasilia, Federal District.Methods: The method used was a descriptive one as well as quantitative boarding. The analyses were collected every two days, and consisted in the observation of the whole process, that is, the collection of the oil as well as its eventual discarding by the UPR. It included the virgin oil, with 03 repetitions of each sample, totalizing 33 fractions of samples for analysis of acidity, peroxide index and iodine index. Key words: Soybean oils in the frying process; Acid greasy; Units of meal production