Colony identity clues for Syntermes grandis (Blattodea: Termitidae) individuals using near-infrared spectroscopy and PLS-DA approach.

Termites are social insects with high species diversity in tropical ecosystems. Multivariate analysis with near-infrared spectroscopy (NIRS) and data interpretation can separate social insects belonging to different colonies of the same species. The objective of this study was to propose the use of discriminant analysis by partial least squares (PLS-DA) combined with NIRS to identify the colonial origin of the Syntermes grandis (Rambur, 1842) (Blattodea: Termitidae) in 2 castes. Six ground S. grandis colonies were identified and mapped; 30 workers and 30 soldier termites in each colony were submitted to spectral measurement with NIRS. PLS-DA applied to the termites' spectral absorbance was used to detect a spectral pattern per S. grandis colony by caste. PLS-DA regression with NIRS proved to be an approach with 99.9% accuracy for identifying the colonial origin of S. grandis workers and 98.3% for soldiers. The methodology showed the importance of qualitatively characterizing the colonial phenotypic response of this species. NIRS is a high-precision approach to identifying the colony origin of S. grandis workers and soldiers. The PLS-DA can be used to design ecological field studies to identify colony territorial competition and foraging behavior of subterranean termite species.

Effects of apigenin on gastric cancer cells.

Gastric Cancer (GC) is one of the most prevalent cancers worldwide. As the currently available therapeutic options are invasive, new and more benign options are being explored. One of which is Apigenin (Api), a natural flavonoid found in fruits and vegetables, such as celery, parsley, garlic, bell pepper and chamomile tea. Api has known anti-inflammatory, -oxidant, and -proliferative proprieties in several diseases and its potential as an anticancer compound has been explored. Here we systematize the available data regarding the effects of Api on GC cells, in terms of cell proliferation, apoptosis, Helicobacter pylori (H. pylori) infection, and molecular targets. From the literature it is possible to conclude that Api inhibits cell growth in a dose- and time-dependent manner, which is accompanied by the reduction of clone formation and induction of apoptosis. This occurs through the Akt/Bad/Bcl2/Bax axis that activates the mitochondrial pathway of apoptosis, resulting in restriction of cell proliferation. Additionally, it seems that the anti-proliferative potential of Api on GC cells is particularly relevant in a more aggressive GC phenotype but can also affect normal gastric cells. This indicate that this flavonoid must be used in low-to-moderate doses to avoid side-effects induced by disturbance of the normal epithelium. In H. Pylori-infected cells, the literature demonstrates that Api reduces inflammation by diminishing the levels of H. pylori colonization, by preventing NF-kB activation and by diminishing the production of reactive oxygen specimens (ROS). Accordingly, in GC Api seems to regulate different hallmarks of cancer, such as cell proliferation, apoptosis, cell migration, inflammation and oxidative stress, demonstrating its potential has an anti-GC compound.

Ocular repercussions in COVID-19 patients: structural changes of the retina and choroid.

BACKGROUND: Neurotropic capabilities of SARS-COVs allow viruses to reach the central nervous system by hematogenous neuronal dissemination. The human retina, as an extension of the Central Nervous System, may have some neurodegenerative and/or vascular modifications related to COVID-19. OBJECTIVES: To evaluate choroidal and inner neural layers in participants previously recovered from COVID-19 compared to the control group using optical coherence tomography. METHODS: With a cross-sectional approach, the sample (n = 96), constituted by patients who have recovered from COVID-19 (n = 56) and healthy participants control group (n = 40) were ophthalmologically characterized. The neurodegenerative and vascular histological assessment was performed using SD-OCT and the mean thickness was measured in Early Treatment Diabetic Retinopathy Study (ETDRS) subfields. Retinal nerve fiber layer, Ganglion cell layer and subfoveal choroidal thickness were obtained through semi-automatic measurement. RESULTS: A total of 40 controls (27 women [67.5%]) and 56 COVID-19 participants (34 women [60.8%]) were included in this first report. There were retinal thickness significant differences in nearly all inner ETDRS subfields: nasal 3 mm (p = .025), I3 (p = .049), and temporal 3 mm (p = .009). Also, a decrease in neural layers was found in the nasal 3 mm (p = .049) and temporal 3 mm (p = .029) during ganglion cell layer assessment. The peripapillary retinal nerve fiber layer thickness was thinner in the COVID-19 group in superior temporal (p = .019), nasal (p = .002), inferior temporal (p = .046) and global (p = .014). Concerning the subfoveal choroidal measurement, an increase was observed in the COVID-19 group (p = .002). CONCLUSION: Participants who had recovered from COVID-19 showed a non-glaucomatous neuropathy trend pattern. We found differences closer to the classic description of the "bow-tie" observed in other neurological as compressive neuropathies at the chiasma location. OCT assessment also showed an increase in choroidal thickness as a result of vascular changes.

Fatal toxoplasmosis in a captive squirrel monkey (Saimiri boliviensis) in Portugal.

New World monkeys are especially vulnerable to develop severe clinical manifestations and succumb to acute toxoplasmosis. This study aimed to describe the histopathological findings and genotypic characterization of the Toxoplasma gondii strain involved in a lethal case occurring in a zoo-housed black-capped squirrel monkey (Saimiri boliviensis) in Portugal. Cyst-like structures suggestive of Sarcocystidae parasites and acute injuries in liver and brain were observed by light microscopy examination. By immunohistochemistry, calprotectin, T. gondii antigen and Iba1 antigen had a positive signaling in lung, liver and brain tissues. Toxoplasma gondii B1, ITS1 and 529 repetitive element fragments amplifications together with the genotyping of 13 microsatellite markers confirmed a systemic T. gondii infection linked to a non-clonal type II strain. This description is consistent to the majority T. gondii strains circulating in Europe.

Use of the Maslach Burnout Inventory Among Public Health Care Professionals: Scoping Review.

BACKGROUND: Work can be considered a source of living, well-being, and socioeconomic development. When the work environment negatively influences individuals, it may trigger emotional disturbances, behavioral problems, chronic stress conditions, and illnesses such as burnout syndrome (BS). Recently, studies on BS have increased and placed a special focus on health care professionals. The prevalence of BS among health professionals is associated with their chronic exposure to human hardship and long working hours without proper rest. These factors have contributed to greater stress and high physical and emotional exhaustion levels. OBJECTIVE: This study aims to identify and map studies using the Maslach Burnout Inventory (MBI) scale to identify burnout syndrome in health professionals working in public health services. METHODS: This scoping review was developed based on the Joanna Briggs Institute (JBI) Reviewers Manual and reported according to the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews). A total of 6 databases were searched to identify relevant studies: Embase, LILACS, MEDLINE or PubMed, PsycInfo, Scopus, and Web of Science. Gray literature was consulted on ProQuest Dissertations and Theses Global, Google Scholar, Brazilian Digital Library of Theses and Dissertations, and Open Access Theses and Dissertations. Additionally, the reference lists were searched to retrieve studies not previously selected. The steps followed in this study were proposed by Arskey and O'Malley and Levac, Colquhoun, and O'Brien: identification of research questions, identification of potential studies, study selection, data extraction and imputation, data analyses and interpretation, and consultation with stakeholders. The detailed methodology was published in a protocol. RESULTS: A total of 55 articles were identified after screening for eligibility criteria, published between 1999 and 2021 in 32 countries. Most reports were published in Brazil, Spain, and China. A total of 22 versions of the MBI were identified, presenting different items, scores, and cutoff points. The included studies had recommendations and implications for clinical practice. The consultation with stakeholders allowed knowledge translation for those interested in BS. CONCLUSIONS: Studies mostly included physicians (34/55, 61.8%) and nurses (24/55, 43.6%), and the original version of MBI was predominantly used. Divergences in BS classification were highlighted, which may be related to MBI cross-cultural adaptations and applications in other countries. This study contributes to the advancement of research regarding burnout syndrome as an occupational illness since it has harmful consequences for workers, health care services, and the quality of care provided to the population.

O ensino na graduação em saúde coletiva: o que dizem os projetos pedagógicos / Undergraduate teaching in public health: what the pedagogical projects say

Resumo Em 2017, para nortear o currículo de ensino, são apresentadas as Diretrizes Curriculares Nacionais do Curso de Graduação em Saúde Coletiva (DCNsCGSC). Com o objetivo de analisar o ensino nos cursos de graduação em saúde coletiva no brasil à luz dos projetos pedagógicos, realizamos um estudo qualitativo e exploratório com abordagem documental, utilizando o método da análise de conteúdo. Depois da análise de 22 projetos, observou-se que a maioria dos cursos segue ou se adequou (31,8%) às DCNsCGSC quanto a carga horária total, estágio, trabalho de conclusão e atividades complementares. A nomenclatura bacharelado em saúde coletiva é a principal denominação (77,3%). A maioria dos cursos está na região Norte (27,%) e em instituições federais (68,5%). Sobre o ensino, foram identificadas 274 citações de componentes obrigatórios com o termo de referência das subáreas da saúde coletiva. Desses, o eixo de políticas, planejamento e gestão em saúde, representa 66,42% (182) dos componentes e as ciências sociais e humanas em saúde 9,50% (26). O estudo elucidou como é realizado o ensino na graduação, agregando reflexões sobre a convergência das DCNsCGSC. Além disso, revela possíveis lacunas dos conteúdos de ensino necessários à formação para a atuação profissional, sobretudo para a consolidação do Sistema Único de Saúde.

Abstract To standardize the teaching curriculum, the National Curriculum Guidelines for the Undergraduate Course in Public Health (DCNsCGSC) were set up in 2017. A qualitative and exploratory study with a documental approach was conducted, using the content analysis method, to analyze teaching in undergraduate courses in Public Health in Brazil in the light of Pedagogical Projects. After analysis of 22 projects, it was observed that the majority of the courses (31.8%) complied with the DCNsCGSC in terms of total workload, internship, final paper, and complementary activities. The term "bachelor's degree in public health" is the main denomination (77.3%). Most courses are in the northern region (27%) and federal institutions (68.5%). In terms of teaching, 274 mentions of mandatory components were identified with the term of reference of the subareas of Public Health. Of these, the areas of health policies, planning, and management represent 66.42% (182) of the components and the social and human sciences in health 9.5% (26). The study elucidated how undergraduate teaching is carried out, adding reflections on the convergence of DCNsCGSC. In addition, it revealed possible gaps in the teaching content necessary for training for professional practice, especially for the consolidation of the Unified Health System.

Graduação em saúde coletiva: o que dizem os projetos pedagógicos / Graduation in public health: what the pedagogical projects say / Graduación en salud colectiva: lo que dicen los proyectos pedagógicos

O objeto de estudo desta dissertação é a formação do sanitarista no nível de graduação. Delimita-se na história dos cursos de graduação em saúde coletiva e no desenho formativo realizado para a nova profissão. Objetiva-se analisar a formação na graduação em saúde coletiva dos cursos existentes no Brasil à luz dos projetos pedagógicos dos cursos. Trata-se de um estudo qualitativo e exploratório com abordagem documental, utilizando o método da análise de conteúdo. Depois da análise de 22 projetos, os resultados demonstraram uma abertura gradual dos cursos, com concentração entre 2008 e 2009, sendo o último aberto em 2019, e que estão organizados em maior número na região Norte, com 27,3%. A maioria dos cursos segue ou se adequou às Diretrizes Curriculares Nacionais do Curso de Graduação em Saúde Coletiva (DCNsCGSC), com predominância de carga horária igual ou superior a 3.200 horas, duração de oito a nove semestres e média de quatro anos, havendo turno noturno em 45,5% dos cursos. A oferta média é de 1.864 vagas/ano, 68% dos cursos são oferecidos por universidades federais, e 77,3% deles recebem a nomenclatura de bacharelado em saúde coletiva. Dos 18 cursos avaliados pelo Ministério da Educação (MEC), 67% são avaliados com conceito 5. A formação permite uma construção do conhecimento de forma gradativa a partir das bases da saúde coletiva. Os cursos articulam-se com os serviços e os sistemas de saúde do Sistema Único de Saúde (SUS) e com a comunidade através de aulas práticas, estágios, pesquisa e projetos de extensão, fortalecendo o contato prático dos alunos com situações reais de saúde da população. Conclui-se que, embora o país esteja vivenciando uma crise na educação superior, os cursos de graduação em saúde coletiva estão na contramão dessa realidade. Apesar de as DCNsCGSC não estarem em vigor devido à não publicação pelo MEC em Diário Oficial, os cursos estão em conformidade com elas. A formação segue as bases da saúde coletiva, de modo que cumpre o objetivo de fortalecimento do SUS para a formação de recursos humanos em saúde.

The object of study of this dissertation is the training of the public health professional at the undergraduate level. It is delimited in the history of undergraduate courses in public health and in the training design carried out for the new profession. The objective is to analyze the undergraduate training in public health of the existing courses in Brazil in the light of the pedagogical projects of the courses. This is a qualitative and exploratory study with a document approach, using the method of content analysis. After analyzing 22 projects, the results showed a gradual opening of courses, with a concentration between 2008 and 2009, with the last one being in 2019, and which were organized in greater numbers in the Northern region, with 27.3%. Most courses follow or fit the National Curriculum Guidelines of the Undergraduate Course in Public Health (DCNCGSC), with a predominance of hours equal to or greater than 3,200 hours, duration of eight to nine semesters, and an average of four years, with night classes in 45.5% of the courses. The average offer is 1,864 places/year, 68% of the courses are offered by federal universities, and 77.3% of them receive the name of bachelor in public health. Of the 18 courses evaluated by the Ministry of Education (MEC), 67% are evaluated with a grade of 5. Training allows a gradual construction of knowledge based on the foundations of public health. The courses are articulated with health services and systems of the Unified Health System (SUS) and with the community with practical classes, internships, research, and extension projects, strengthening students' practical contact with real health situations in the population. Therefore, although the country is experiencing a crisis in higher education, the undergraduate courses in public health are going against this reality. Although the DCNsCGSC are not in force due to the non-publication by the MEC in the Official Gazette, the courses are in accordance with them. The training follows the basis of collective health so that it fulfills the objective of strengthening the SUS for the training of human resources in health.

El objeto de estudio de esta tesis es la formación de profesionales de la salud pública en el grado. Se hace una delimitación de la trayectoria de los cursos de grado en salud colectiva y el diseño formativo realizado para la nueva profesión. La investigación pretende analizar la formación de grado en salud colectiva en Brasil a partir de los proyectos pedagógicos de los cursos. Se trata de un estudio cualitativo y exploratorio, de enfoque documental, y con el método de análisis de contenido. Del análisis de 22 proyectos, los resultados muestran que hubo una ampliación gradual de los cursos en el período entre 2008 y 2009, la última se llevó a cabo en 2019, y que entre las regiones brasileñas donde se concentran destaca más el Norte, con un 27,3%. La mayoría de los cursos siguen o se ajustan a las Directrices Curriculares Nacionales de la Carrera de Grado en Salud Colectiva (DCNsCGSC), tienen carga horaria igual o superior a 3.200 horas, con una duración de ocho a nueve semestres y un promedio de cuatro años, con jornada nocturna en el 45,5%. La oferta promedio es de 1.864 vacantes/año, el 68% de los cursos son en universidades federales y el 77,3% llevan el nombre Licenciatura en Salud Colectiva. De los 18 cursos evaluados por el Ministerio de Educación (MEC), el 67% recibieron calificación 5. La formación permite construir paulatinamente conocimientos a partir de las bases de la salud colectiva. Los cursos se articulan con los servicios y redes de salud del Sistema Único de Salud (SUS) brasileño y con la comunidad mediante clases prácticas, pasantías, proyectos de investigación y extensión, fortaleciendo el contacto de los alumnos con situaciones reales de salud de la población. Se concluye que, aunque el país viva una crisis en la educación superior, las carreras de grado en salud colectiva van en sentido contrario a esta realidad. Si bien las DCNsCGSC no se encuentran vigentes todavía, porque hace falta que el MEC las publique en el Diario Oficial, los cursos están de acuerdo con ellas. La formación sigue las bases de la salud colectiva, de modo que cumpla el objetivo de fortalecer el SUS para la formación de recursos humanos en salud.

Detection of Abnormal Prion Protein by Immunohistochemistry.

Abnormal prion proteins (PrPSc) are the disease-associated isoform of cellular prion protein and diagnostic markers of transmissible spongiform encephalopathies (TSEs). These neurodegenerative diseases affect humans and several animal species and include scrapie, zoonotic bovine spongiform encephalopathy (BSE), chronic wasting disease of cervids (CWD), and the newly identified camel prion disease (CPD). Diagnosis of TSEs relies on immunodetection of PrPSc by application of both immunohistochemistry (IHC) and western immunoblot methods (WB) on encephalon tissues, namely, the brainstem (obex level). IHC is a widely used method that uses primary antibodies (monoclonal or polyclonal) against antigens of interest in cells of a tissue section. The antibody-antigen binding can be visualized by a color reaction that remains localized in the area of the tissue or cell where the antibody was targeted. As such, in prion diseases, as in other fields of research, the immunohistochemistry techniques are not solely used for diagnostic purposes but also in pathogenesis studies. Such studies involve detecting the PrPSc patterns and types from those previously described to identify the new prion strains. As BSE can infect humans, it is recommended that biosafety laboratory level-3 (BSL-3) facilities and/or practices are used to handle cattle, small ruminants, and cervid samples included in the TSE surveillance. Additionally, containment and prion-dedicated equipment are recommended, whenever possible, to limit contamination. The PrPSc IHC procedure consists of a formic acid epitope-demasking step also acting as a prion inactivation measure, as formalin-fixed and paraffin-embedded tissues used in this technique remain infectious. When interpreting the results, care must be taken to distinguish non-specific immunolabeling from target labeling. For this purpose, it is important to recognize artifacts of immunolabeling obtained in known TSE-negative control animals to differentiate those from specific PrPSc immunolabeling types, which can vary between TSE strains, host species, and prnp genotype, further described herein.

[Undergraduate teaching in public health: what the pedagogical projects say]. / O ensino na graduação em saúde coletiva: o que dizem os projetos pedagógicos.

To standardize the teaching curriculum, the National Curriculum Guidelines for the Undergraduate Course in Public Health (DCNsCGSC) were set up in 2017. A qualitative and exploratory study with a documental approach was conducted, using the content analysis method, to analyze teaching in undergraduate courses in Public Health in Brazil in the light of Pedagogical Projects. After analysis of 22 projects, it was observed that the majority of the courses (31.8%) complied with the DCNsCGSC in terms of total workload, internship, final paper, and complementary activities. The term "bachelor's degree in public health" is the main denomination (77.3%). Most courses are in the northern region (27%) and federal institutions (68.5%). In terms of teaching, 274 mentions of mandatory components were identified with the term of reference of the subareas of Public Health. Of these, the areas of health policies, planning, and management represent 66.42% (182) of the components and the social and human sciences in health 9.5% (26). The study elucidated how undergraduate teaching is carried out, adding reflections on the convergence of DCNsCGSC. In addition, it revealed possible gaps in the teaching content necessary for training for professional practice, especially for the consolidation of the Unified Health System.

Em 2017, para nortear o currículo de ensino, são apresentadas as Diretrizes Curriculares Nacionais do Curso de Graduação em Saúde Coletiva (DCNsCGSC). Com o objetivo de analisar o ensino nos cursos de graduação em saúde coletiva no brasil à luz dos projetos pedagógicos, realizamos um estudo qualitativo e exploratório com abordagem documental, utilizando o método da análise de conteúdo. Depois da análise de 22 projetos, observou-se que a maioria dos cursos segue ou se adequou (31,8%) às DCNsCGSC quanto a carga horária total, estágio, trabalho de conclusão e atividades complementares. A nomenclatura bacharelado em saúde coletiva é a principal denominação (77,3%). A maioria dos cursos está na região Norte (27,%) e em instituições federais (68,5%). Sobre o ensino, foram identificadas 274 citações de componentes obrigatórios com o termo de referência das subáreas da saúde coletiva. Desses, o eixo de políticas, planejamento e gestão em saúde, representa 66,42% (182) dos componentes e as ciências sociais e humanas em saúde 9,50% (26). O estudo elucidou como é realizado o ensino na graduação, agregando reflexões sobre a convergência das DCNsCGSC. Além disso, revela possíveis lacunas dos conteúdos de ensino necessários à formação para a atuação profissional, sobretudo para a consolidação do Sistema Único de Saúde.

Estimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deer.

Among the transmissible spongiform encephalopathies (TSEs), chronic wasting disease (CWD) in cervids is now a rising concern in wildlife within Europe, after the detection of the first case in Norway in 2016, in a wild reindeer and until June 2022 a total of 34 cases were described in Norway, Sweden and Finland. The definite diagnosis is post-mortem, performed in target areas of the brain and lymph nodes. Samples are first screened using a rapid test and, if positive, confirmed by immunohistochemistry and Western immunoblotting. The study of the genetics of the prion protein gene, PRNP, has been proved to be a valuable tool for determining the relative susceptibility to TSEs. In the present study, the exon 3 of PRNP gene of 143 samples from red deer (Cervus elaphus) and fallow deer (Dama dama) of Portugal was analysed. Three single nucleotide polymorphisms (SNPs) were found in red deer - codon A136A, codon T98A, codon Q226E - and no sequence variation was detected in fallow deer. The low genetic diversity found in our samples is compatible with previous studies in Europe. The comparison with results from North America suggests that the free-ranging deer from our study may present susceptibility to CWD, although lack of experimental data and the necessity of continuous survey are necessary to evaluate these populations.

Use of the Maslach Burnout Inventory Among Public Health Care Professionals: Protocol for a Scoping Review.

BACKGROUND: Burnout syndrome is a chronic response to stressors in the workplace. It is characterized by emotional exhaustion and physical and mental burnout and may lead to high employee turnover, work absenteeism, and increased occupational accidents. Most studies use the Maslach Burnout Inventory (MBI) to identify burnout and implement preventive actions and treatments. OBJECTIVE: This study presents a scoping review protocol to identify and map studies that used MBI to assess burnout syndrome in health care professionals working in public health services. METHODS: This scoping review protocol follows the Joanna Briggs Institute reviewers' manual, and this protocol consists of 6 stages: identifying the research question, identifying relevant studies, study selection, data extraction and coding, analysis and interpretation of results, and consultation with stakeholders. We will conduct searches in Embase, LILACS, PubMed/MEDLINE, PsycINFO, Scopus, Web of Science databases, and gray literature. The main research question is as follows: how is MBI used to identify burnout syndrome in health care professionals working in public health services? Inclusion criteria will comprise qualitative and quantitative studies using MBI to identify burnout syndrome in health care professionals working in public health services and no restrictions in language and publication dates. Data will be extracted using a spreadsheet adapted from the Joanna Briggs Institute model. Quantitative and qualitative data will be analyzed using descriptive statistics and thematic analysis, respectively. The consultation with stakeholders will be essential for increasing the knowledge about MBI, identifying new evidence, and developing future strategies to guide public policies preventing burnout syndrome in health care professionals working in public services. RESULTS: This protocol will guide a scoping review to identify and map studies that used MBI to identify burnout syndrome in health care professionals working in public health services. The results of this review may be useful to public health care professionals, managers, policymakers, and the general population because these findings will help understand the validated, translated, and adapted versions of MBI and domains, number of items, Likert scales, and cutoff points or the latent profile analysis most used in the literature. Furthermore, possible research gaps may be identified to guide future studies. All information regarding the stages of the scoping review favor its transparency and allow it to be methodologically replicated according to the principles of open science, thereby reducing the risk of bias and data duplication. CONCLUSIONS: This study may reveal the multiplicity of scales described in the literature and the different forms of assessing burnout syndrome in health care professionals. This study may help to standardize the assessment of burnout syndrome in health care professionals working in public health services and contribute to the discussion and knowledge dissemination about burnout syndrome and mental health in this population. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/42338.

Risk Factors for Development of Rectal Neuroendocrine Tumors Longer Than 9 mm: Retrospective Cohort.

BACKGROUND: Rectal neuroendocrine tumors (R-NET) represent the most frequent of gastroenteropancreatic neuroendocrine neoplasms (NEN-GEP) according to the United States Surveillance, Epidemiology, and End Results database. With an annual percentage of occurrence increasing to 8.2% of all rectal neoplasms, R-NET affect less than 2% and are reported in only 0.05% to 0.07% of patients undergoing colorectal cancer (CRC) screening. The primary objective of this study was to assess the risk factors associated with R-NET greater than 10 mm. As a secondary objective, it was also aimed to evaluate the response to endoscopic treatment. METHODS: This was a retrospective study, using data collected through the analysis of medical records of colonoscopies performed from January 2008 to December 2014. Records of polypectomies were identified, and the results were searched for pathological findings of R-NET. We also gathered epidemiological data and outcomes as risk factors for lesions greater than or equal to 10 mm, with local and distant recurrence. RESULTS: During the study period, 18 218 colonoscopies were performed and 10 865 polypoid lesions were detected and removed, 20 with R-NET anatomopathology. The detection rate was 0.1%. The risk factors associated with major lesions were Japanese ethnicity, the lack of previous cancer diagnosis, and a Ki67 index > 2%. The mean follow-up was 56.6 months, and there was no local lymph node recurrence or distant relapse. CONCLUSION: This study concludes that endoscopic resection is a good and effective method for treatment of Grade 1 rectal NET smaller than 11 mm, with high cure rates and low rates of local or distant relapse.

Neuropathology of Animal Prion Diseases.

Transmissible Spongiform Encephalopathies (TSEs) or prion diseases are a fatal group of infectious, inherited and spontaneous neurodegenerative diseases affecting human and animals. They are caused by the conversion of cellular prion protein (PrPC) into a misfolded pathological isoform (PrPSc or prion- proteinaceous infectious particle) that self-propagates by conformational conversion of PrPC. Yet by an unknown mechanism, PrPC can fold into different PrPSc conformers that may result in different prion strains that display specific disease phenotype (incubation time, clinical signs and lesion profile). Although the pathways for neurodegeneration as well as the involvement of brain inflammation in these diseases are not well understood, the spongiform changes, neuronal loss, gliosis and accumulation of PrPSc are the characteristic neuropathological lesions. Scrapie affecting small ruminants was the first identified TSE and has been considered the archetype of prion diseases, though atypical and new animal prion diseases continue to emerge highlighting the importance to investigate the lesion profile in naturally affected animals. In this report, we review the neuropathology and the neuroinflammation of animal prion diseases in natural hosts from scrapie, going through the zoonotic bovine spongiform encephalopathy (BSE), the chronic wasting disease (CWD) to the newly identified camel prion disease (CPD).

Recombinant myxoma virus infection associated with high mortality in rabbit farming (Oryctolagus cuniculus).

Myxomatosis is an emergent disease in the Iberian hare, having been considered a rabbit disease for decades. Genome sequencing of the strains obtained from Iberian hares with myxomatosis showed these to be distinct from the classical ones that circulated in rabbits since the virus introduction in Europe, in 1952. The main genomic difference in this natural recombinant hare myxoma virus (ha-MYXV) is the presence of an additional 2.8 kb region disrupting the M009L gene and adding a set of genes homologous to the myxoma virus (MYXV) genes M060R, M061R, M064R, M065R and M066R originated in Poxviruses. After the emergence of this recombinant virus (ha-MYXV) in hares, in the summer of 2019, the ha-MYXV was not detected in rabbit surveys, suggesting an apparent species segregation with the MYXV classic strains persistently circulating in rabbits. Recently, a group of six unvaccinated European rabbits (Oryctolagus cuniculus cuniculus) from a backyard rabbitry in South Portugal developed signs of myxomatosis (anorexia, dyspnoea, oedema of eyelids, head, ears, external genitals and anus, and skin myxomas in the base of the ears). Five of them died within 24-48 hr of symptom onset. Molecular analysis revealed that only the recombinant MYXV was present. This is the first documented report of a recombinant hare myxoma virus in farm rabbits associated with high mortality, which increases the concern for the future of both the Iberian hare and wild rabbits and questions the safety of the rabbit industry. This highlights the urgent need to evaluate the efficacy of available vaccines against this new MYXV.

Estudo bibliométrico da produção científica da Revista Brasileira de Geriatria e Gerontologia entre 2014 e 2019 / Bibliometric study of the scientific production of Brazilian Journal of Geriatrics and Gerontology between 2014 and 2019

Resumo Objetivo Descrever e analisar o perfil das publicações da Revista Brasileira de Geriatria e Gerontologia (RBGG) entre 2014 e 2019. Método Trata-se de um estudo bibliométrico da produção científica da RBGG, durante o período de janeiro de 2014 a dezembro de 2019. A coleta de dados foi realizada por quatro pesquisadoras independentes, previamente treinadas e orientadas para a uniformização desse processo. Os dados coletados foram dispostos numa planilha no Microsoft Excel® e analisados no programa Stata versão 10.0. As variáveis analisadas foram organizadas em quatro eixos: identificação da publicação; tipo de estudo; autores; e processo editorial. Resultados Publicou-se um total de 504 estudos de 2014 a 2019, destes, 75% são artigos originais e 13,49% de revisão, 95,4% dos autores eram de nacionalidade brasileira e as instituições de afiliação eram majoritariamente do Sudeste (41,8%) e Sul (28,68%). A abordagem quantitativa foi predominante (70,5%) e as temáticas que se destacaram foram saúde pública (33,1%), doenças (19,9%) e assistência em saúde (19,4%). O tempo de aprovação apresentou uma redução média de quatro meses e o de publicação três meses. Conclusão Houve um aumento no número de publicações durante o período analisado, com destaque para artigos originais e estudos quantitativos. A diversidade de temas revela a tendência mais abrangente, para além daquelas centradas na doença, e mais para processos biopsicossociais e comportamentais, conforme verificado nos estudos em saúde pública. O processo editorial tem se mostrado célere com redução expressiva no tempo entre o recebimento e a publicação, e aumento no quantitativo de aprovação dos artigos recebidos.

Abstract Objective To describe and analyze the profile of publications of the Revista Brasileira de Geriatria e Gerontologia (RBGG) from 2014 to 2019. Method This is a bibliometric study of the scientific production of RBGG, during the period from January 2014 to December 2019. Data collection was performed by four independent researchers, previously trained and oriented to standardize this process. The collected data were arranged in a spreadsheet in Microsoft Excel® and analyzed using the Stata version 10.0 program. The analyzed variables were organized into four axes: Publication identification; Kind of study; Authors; and Editorial process. Results A total of 504 studies were published from 2014 to 2019, of which 75% are original articles and 13.49% reviews, 95.4% of the authors were Brazilian nationals and affiliation institutions were mostly in the Southeast (41.8%) and South (28.68%). The quantitative approach (70.5%) was predominant and the themes that stood out were public health (33.1%), diseases (19.9%), and health care (19.4%). The approval time showed a reduction lasted an average of four months and the publication three months. Conclusion There was an increase in the number of publications during the analyzed period, with emphasis on original articles and quantitative studies. The diversity of themes reveals the most common trend, beyond those centered on the disease, and more towards biopsychosocial and behavioral processes, as verified in studies in public health. The editorial process was rapid with a significant reduction in the time between receipt and publication, and an increase in the amount of approval of the articles received.

Detection of recombinant Hare Myxoma Virus in wild rabbits (Oryctolagus cuniculus algirus).

In late 2018, an epidemic myxomatosis outbreak emerged on the Iberian Peninsula leading to high mortality in Iberian hare populations. A recombinant Myxoma virus (strains MYXV-Tol and ha-MYXV) was rapidly identified, harbouring a 2.8 kbp insertion containing evolved duplicates of M060L, M061L, M064L, and M065L genes from myxoma virus (MYXV) or other Poxviruses. Since 2017, 1616 rabbits and 125 hares were tested by a qPCR directed to M000.5L/R gene, conserved in MYXV and MYXV-Tol/ha-MYXV strains. A subset of the positive samples (20%) from both species was tested for the insert with MYXV being detected in rabbits and the recombinant MYXV in hares. Recently, three wild rabbits were found dead South of mainland Portugal, showing skin oedema and pulmonary lesions that tested positive for the 2.8 kbp insert. Sequencing analysis showed 100% similarity with the insert sequences described in Iberian hares from Spain. Viral particles were observed in the lungs and eyelids of rabbits by electron microscopy, and isolation in RK13 cells attested virus infectivity. Despite that the analysis of complete genomes may predict the recombinant MYXV strains' ability to infect rabbit, routine analyses showed species segregation for the circulation of MYXV and recombinant MYXV in wild rabbit and in Iberian hares, respectively. This study demonstrates, however, that recombinant MYXV can effectively infect and cause myxomatosis in wild rabbits and domestic rabbits, raising serious concerns for the future of the Iberian wild leporids while emphasises the need for the continuous monitoring of MYXV and recombinant MYXV in both species.

Endogenous endophthalmitis due to Kingella Kingae infectious endocarditis / Endoftalmite endógena por endocardite infecciosa devido a Kingella Kingae

Abstract This report presents a rare case of endogenous endophthalmitis due to Kingella kingae infectious endocarditis. Endogenous endophthalmitis is a rare condition that has a systemic underlying cause, with hematogenic dissemination of a pathogen that will eventually reach and infect the eye. In this article, we present a case of a 54-year-old woman with fever, chills and decreased visual acuity and pain in the right eye. The slit-lamp exam showed conjunctival injection, anterior chamber reaction with a great amount of fibrinous material obscuring her visual axis. Ultrasound echography revealed profuse exudates and scarce membranous formation in the posterior segment. Blood culture was positive for Kingella kingae, and the patient was treated with intravenous ceftriaxone, along with topic dexamethasone and mydriatic. After 15 days of intravenous antibiotic therapy, the patient exhibited best visual acuity of 20/60. Endogenous endophthalmitis is an ocular emergency that demands quick diagnosis and aggressive intervention in order to preserve vision. Therefore, it is important to recognize its signs and symptoms with no retard.

Resumo O presente relato apresenta um raro caso de endoftalmite endógena por endocardite devido à Kingella kingae. Endoftalmite endógena é uma doença pouco comum com uma causa sistêmica subjacente. A disseminação hematogênica de um microrganismo infeccioso leva à infecção ocular. Nesse artigo, apresentamos o caso de uma mulher com 54 anos, febre, calafrios, baixa da acuidade visual e dor em olho direito. Ao exame na lâmpada de fenda apresentava injeção conjuntival, reação de câmara anterior e acúmulo de fibrina no eixo visual. Ultrassonografia revelou exsudatos profusos e escassa formação membranosa em segmento posterior.A hemocultura foi positiva para Kingella kingae e a paciente foi tratada com ceftriaxone venoso conjuntamente com dexametasona e midriático tópicos. Após 15 dias de terapia antibiótica endovenosa, a paciente apresentou acuidade visual corrigida de 20/60. Endoftalmite endógena é uma emergência ocular que demanda rápido diagnóstico e intervenção agressiva para preservar a visão. Portanto, é importante o reconhecimento precoce dos sinais e sintomas.

Ocorrência e fatores de risco para lesões por pressão em centros de terapia intensiva / Occurrence and risk factors for pressure injuries in intensive care centers

Objetivo: descrever a frequência e os fatores de risco para o desenvolvimento de lesões por pressão em clientes de Centros de Terapia Intensiva. Método: estudo quantitativo, transversal, realizado em uma amostra de 104 clientes de uma população de 936 sujeitos de dois hospitais públicos de ensino, atendidos entre os meses de março a junho de 2016. Os dados foram coletados com uso de um formulário de observação baseado em diretrizes nacionais e internacionais, e a análise estatística descritiva foi realizada com adoção de um nível de significância de 5%. Os resultados são apresentados em tabelas em valores absolutos e relativos, e cálculos para verificação de associação entre as variáveis. Resultados: dentre as variáveis estudadas, a idade foi igual ou maior a 60 anos, a presença de hipertermia e de pele edemaciada foram as que apresentaram significância estatística com a ocorrência de LP. Conclusão: os fatores de risco identificados como significativos para a ocorrência de LP podem ser prevenidos, portanto, o envolvimento da equipe de enfermagem é fundamental na prevenção dessas lesões. (AU)

Prevenção de lesão por pressão: ações prescritas por enfermeiros de centros de terapia intensiva / Prevención de lesión por presión: acciones prescritas por enfermeros de centros de terapia intensiva / Prevention of pressure injuries: actions prescribed by intensive care unit nurses

RESUMO Objetivo: descrever as ações de enfermagem prescritas por enfermeiros para a prevenção de lesões por pressão e sua ocorrência em centros de terapia intensiva. Método: estudo transversal, descritivo e analítico, de abordagem quantitativa conduzido em duas instituições hospitalares de ensino de Campo Grande, Brasil. A coleta ocorreu entre março e junho de 2016 com 104 participantes. Os dados foram submetidos à análise estatística. Resultados: foi encontrada associação estatística entre as ações de mudança de decúbito, aplicação de cobertura hidrocoloide em região sacral, realização de higiene externa, troca de fixação do cateter orotraqueal e/ou cateter nasoenteral e inspeção da pele com a ausência de lesões por pressão. A ocorrência de lesões por pressão foi encontrada em 49% dos clientes em ambas as instituições. Conclusão: a elaboração e implementação de protocolos, o acompanhamento dos registros e dos grupos de maior risco são estratégias que direcionam a prescrição de ações preventivas adequadas para lesões por pressão.

RESUMEN Objetivo: describir las acciones de enfermería prescritas por enfermeros para la prevención de lesiones por presión y su ocurrencia en centros de terapia intensiva. Método: estudio transversal, descriptivo y analítico, de abordaje cuantitativo conducido en dos instituciones hospitalarias de enseñanza de Campo Grande, Brasil. La recolección ocurrió entre marzo y junio de 2016 comm 104 participantes. Los datos se sometieron al análisis estadístico. Resultados: se encontró asociación estadística entre las acciones de cambio de decúbito, aplicación de cobertura hidrocoloide en región sacral, realización de higiene externa, cambio de fijación del catéter orotraqueal y/o catéter nasoenteral e inspección de la piel con la ausencia de lesiones por presión. La presencia de lesiones por presión fue encontrada en el 49% de los clientes en ambas instituciones. Conclusión: la elaboración e implementación de protocolos, el seguimiento de los registros y de los grupos de mayor riesgo son estrategias que orientan la prescripción de acciones preventivas adecuadas para lesiones por presión.

ABSTRACT Objective: to describe the nursing actions prescribed by nurses for the prevention of pressure injuries and their occurrence in intensive care units. Method: a cross-sectional, descriptive, analytical, quantitative study was conducted in two teaching hospitals in Campo Grande, Brazil. The collection occurred between March and June 2016, with 104 participants. The data were submitted to statistical analysis. Results: a statistical association was found between the actions of change of decubitus, application of hydrocolloid dressings on the sacral region, performance of external hygiene, change of the orotracheal catheter and/or nasoenteral catheter fixation device and inspection of the skin without pressure injuries. The occurrence of pressure injuries was found in 49% of the clients in both institutions. Conclusion: the elaboration and implementation of procedures and the monitoring of reports and of higher risk groups are strategies that direct the prescription of appropriate preventive actions for pressure injuries.

Life quality assessment of patients after phacoemulsification or extracapsular cataract extraction.

PURPOSE: To study the quality of life, treatment outcomes, and satisfaction in patients who have undergone cataract surgery Methods: This comparative case series study was conducted at the Ophthalmology Service of the Bettina Ferro de Souza University Hospital, Belém, Pará, Brazil. Totally, 60 patients with cataract were included; 50% underwent conventional extracapsular cataract extraction (ECEE) and 50% underwent cataract extraction by phacoemulsification (PHACO). Patients were interviewed using the Visual Function 14 (VF-14) questionnaire to determine the quality of life before and 30 days after surgery. The results of ophthalmological examination were recorded in the patients' files and were available throughout this study. One-way ANOVA, Tukey's post-hoc comparison, and the sign test were used for statistical analyses. RESULTS: The mean VF-14 satisfaction index was 38.0 and 89.4 before and after surgery, respectively, for the ECEE group and 47.0 and 94.1, respectively, for the PHACO group. The improvement in patient quality of life after surgery was significant in both groups (p<0.0001), with a similar amount of improvement in both groups. CONCLUSIONS: The observed improvement in quality of life was significant (p<0.0001) and directly related to patient satisfaction with surgical outcomes, which was also significant (p<0.0001) as assessed using the VF-14. Satisfaction and quality of life are individual factors; consequently, patient responses to questions regarding improvements in the ability to perform each activity are subjective and depend uniquely on individual perception.