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Conservation tillage practices, including reduced tillage (RT), no-tillage (NT) and straw return (SR), have been widely adopted to enhance soil organic carbon density (PSOC) and improve the soil quality while mitigating the negative environmental impacts of intensive farming. However, current studies on the effects of these practices on SOC sequestration and N2O flux show considerable variability, making it challenging to draw definitive conclusions about the individual and combined impacts of conservation tillage practices and introducing substantial uncertainty in estimating the agricultural sector's potential to mitigate climate change. To address this gap, we conducted a meta-analysis of 902 pairwise comparisons from 90 peer-reviewed publications to evaluate the effects of five conservation tillage practices (straw return (SR), reduced tillage (RT), no-tillage (NT), straw return combined with tillage reduction (SR + RT) and straw returning combined with no-tillage (SR + NT)) on C sequestration and N2O emissions from agricultural soils. The results show that SR was the most effective practice for increasing SOC content (23.7 %), followed by RT + SR (5.5 %) and NT + SR (4.4 %). Additionally, RT (12.3 %) and NT (14.3 %) significantly reduced soil N2O emissions. This study also identified key drivers, including climatic factors, soil properties, and agricultural management practices, that influence SOC content and N2O emissions under different conservation tillage practices. For example, the mean annual precipitation, mean annual temperature, soil type, pH, soil total nitrogen content, N application rate, and experiment duration were identified as the key factors affecting SOC content and N2O emissions Specifically, suitable temperature, lower rainfall and alkaline soil environment significantly enhanced the C sequestration efficiency of SR, while suitable climatic conditions and soil texture combined with an alkaline environment contributed to a significant reduction in long-term NT soil N2O emissions. These results provide a robust scientific foundation for the strategic implementation of conservation tillage to reduce greenhouse gas emissions, mitigate global warming, and enhance soil C sequestration capacity.
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Cell membranes are primarily composed of lipids, membrane proteins, and carbohydrates, and the related studies of membrane components and structures at different stages of disease development, especially membrane proteins, are of great significance. Here, we investigate the chemical signature profiles of cell membranes as biomarkers for cancer cells via label-free surface-enhanced Raman scattering (SERS). A magnetic plasmonic nanoprobe was proposed by decorating magnetic beads with silver nanoparticles, allowing self-driven cell membrane-targeted accumulation within 5 min. SERS profiles of three types of breast cells were achieved under the plasmon enhancement effect of these nanoprobes. Membrane fingerprint spectra from breast cell lines were further classified with the convolutional neural network model, which perfectly distinguished between two breast cancer subtypes. We further tested various clinical samples using this method and obtained fingerprint spectra from primary cells and frozen slices. This study presents a practical, user-friendly approach for label-free and in situ analysis of cell membranes, which can work for early tumor screening and treatment assessment for tumors reliant on the Molecular profiles of cell membranes. Additionally, this method can be applied universally to explore cell membrane components of other cells, thus assisting Human Cell Atlas.
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Fertilizer application is the basis for ensuring high yield, high quality and high efficiency of farmland. In order to meet the demand for food with the increasing of population, the application of nitrogen fertilizer will be further increased, which will lead to problems such as N2O emission and nitrogen loss from farmland, it will easily deteriorate the soil and water environment of farmland, and will not conducive to the sustainable development of modern agriculture. However, optimizing fertilizer management is an important way to solve this problem. While, due to the differences in the study conditions (geographical location, environmental conditions, experimental design, etc.), leading to the results obtained in the literatures about the N2O emission with different nitrogen fertilizer application strategies have significant differences, which requiring further comprehensive quantitative analysis. Therefore, we analyzed the effects of nitrogen fertilizer application strategies (different fertilizer types and fertilizer application rates) on N2O emissions from the fields (rice, wheat and maize) based on the Meta-analysis using 67 published studies (including 1289 comparisons). For the three crops, inorganic fertilizer application significantly increased on-farm N2O emissions by 19.7-101.05% for all three; and organic fertilizer increased N2O emissions by 28.16% and 69.44% in wheat and maize fields, respectively, but the application of organic fertilizer in rice field significantly reduced N2O emissions by 58.1%. The results showed that overall, the application of inorganic fertilizers resulted in higher N2O emissions from farmland compared to the application of organic fertilizers. In addition, in this study, the average annual temperature, annual precipitation, soil type, pH, soil total nitrogen content, soil organic carbon content, and soil bulk weight were used as the main influencing factors of N2O emission under nitrogen fertilizer strategies, and the results of the study can provide a reference for the development of integrated management measures to control greenhouse gas emissions from agricultural soils.
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Agricultura , Fertilizantes , Óxido Nitroso , Oryza , Triticum , Zea mays , Óxido Nitroso/análisis , Fertilizantes/análisis , Zea mays/crecimiento & desarrollo , Triticum/crecimiento & desarrollo , Agricultura/métodos , Oryza/crecimiento & desarrollo , Nitrógeno/análisis , Productos Agrícolas/crecimiento & desarrollo , Suelo/química , GranjasRESUMEN
Cytokine expression is an important biomarker in understanding hypoxia microenvironments in tumor growth and metastasis. In-droplet-based immunoassays performed above the target cell membrane were employed to track the cytokines of single cells with the aid of three types of immuno-nanoprobes (one capture nanoprobe and two reporter nanoprobes). Single cells and nanoprobes were co-packaged in water-in-oil microdroplets (about 100 µm in diameter) using a cross-shaped microfluidic chip. In each droplet, capture nanoprobes would be first fixed to the cell surface by linking to membrane proteins that have been streptavidinized. Then, the capture nanoprobes can collect cell-secreted cytokines (VEGF and IL-8) by the antibodies, followed by two reporter nanoprobes that emit distinguishable fluorescence. Fluorescence imaging was utilized to record the signal outputs of two reporter probes, which reflect cytokine expressions secreted by a single tumor cell. The cytokine levels at different degrees of hypoxia induction were assessed. Multiple chemometric methods were adopted to distinguish differences in the secretion of two cytokines and the results demonstrated a positive correlation. This study developed an in-droplet, dual-target, simultaneous biosensing strategy for a single cell, which is helpful for understanding the impacts of hypoxia microenvironments on cell cytokines that are vital for assessing early cancer diagnosis and prognosis.
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Análisis de la Célula Individual , Inmunoensayo/métodos , Humanos , Análisis de la Célula Individual/métodos , Citocinas/metabolismo , Citocinas/análisis , Interleucina-8/análisis , Interleucina-8/metabolismo , Factor A de Crecimiento Endotelial Vascular/análisis , Factor A de Crecimiento Endotelial Vascular/metabolismo , Dispositivos Laboratorio en un Chip , Hipoxia de la Célula , Hipoxia/metabolismoRESUMEN
Application of biochar has been demonstrated to be a successful strategy for boosting soil carbon sequestration and altering the agricultural soil carbon cycle. However, in the studies involving biochar worldwide, the effects of different types of biochar on the soil carbon component response direction and increase are not consistent. Therefore, to assess the effects of applying four types of biochar during the soil carbon cycle on carbon components on a farmland, we performed a meta-analysis of 1150 comparisons from 86 peer-reviewed publications. Generally speaking, the types of biochar raw materials have a significant impact on soil carbon cycle. The application of chaff biochar significantly inhibited (10.0 %) soil respiration, while the application of manure biochar (47.0 %), straw biochar (11.2 %) and wood biochar (8.7 %) showed a strong promotion effect on CO2 emission. In addition, although the soil organic C, microbial biomass C and dissolved organic C all had positive responses to the application of the four biochar types, the degree and increase in their response varied greatly due to the differences in biomass raw materials. Moreover, by increasing the biochar rates applied to coarse-textured soils with low average annual rainfall and an average temperature under controlled circumstances, the relative increase in SOC was encouraged. Meanwhile, applying low temperature pyrolytic biochar (≤400 °C) at a lower rate (<25 t/ha) in the long-term experiment (>3 years) is more beneficial to soil C sequestration and emission reduction. Hence, climatic conditions, agricultural management practices, and initial soil properties jointly constrained and influenced the ability of biochar to alter the soil C cycle. Based on this, our research offers a fresh viewpoint for making a profound study biochar-enhanced soil C cycle.
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Agricultura , Ciclo del Carbono , Carbón Orgánico , Suelo , Carbón Orgánico/química , Suelo/química , Agricultura/métodos , Granjas , Carbono/análisis , Secuestro de CarbonoRESUMEN
Based on current implantable devices, a battery's rigidity and large size makes it prone to immune rejection and wound incisions. Additionally, it is limited by its finite lifespan, which hinders long-term usage. These limitations greatly restrict the development of implantable medical device systems towards miniaturization and minimally invasive approaches. Consequently, obtaining high-fidelity and stable biological signals from the target tissue area of the organism remains challenging. Therefore, there is a need to develop wireless power transmission technology. In this paper, we propose a wireless micro energy transfer method based on MEMS micro coils for charging implantable devices. Through simulation calculations, we first investigate the influence of coaxial distance, horizontal displacement, and rotation angle between the MEMS micro coil and the transmitting coil on power transmission. Subsequently, we utilize micro nanofabrication technology to create a MEMS micro spiral copper coil with a line width, thickness, and spacing of 50 µm and a total of five turns. Finally, we conduct wireless power transmission tests on the coil. The results show that, when the transmitting coil and the receiving coil are 10 mm apart and the operating frequency is 100 kHz, the power of the wireless power transmission system reaches 45 µW. This power level is sufficient to meet the power supply requirements of implantable pacemakers. Therefore, this technology holds great potential for applications in the field of wireless power transmission for implantable medical devices, including pacemakers and brain neurostimulators.
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Kiwifruits are often exposed to various temperature fluctuations (TFs) during postharvest transportation and storage. To evaluate the effect of TFs on the qualities of kiwifruits during storage, kiwifruits were stored at 2 °C, 2 °C or 5 °C (TF2 °C-5 °C, alternating every 12 h), 2 °C or 7 °C (TF2 °C-7 °C, alternating every 12 h) for 3 d before long time storage at 2 °C. Observations revealed that kiwifruits stored at a constant 2 °C showed the lowest loss of weight and vitamin C because of minimized ethylene production and respiratory rate compared with that of TF2 °C-5 °C and TF2 °C-7 °C. Moreover, the results of RT-qPCR verified that the expression levels of genes encoding polygalacturonase, ß-galacturonidase, and pectin methylesterase were significantly increased by the treatment of TF. Hence, TF accelerated the degradation of cell walls, softening, translucency, and relative conductivity of the flesh of kiwifruits. In addition, the impact of TF2 °C-7 °C on kiwifruits was more significant relative to TF2 °C-5 °C. The present study provides a theoretical basis for kiwifruit during cold storage.
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The binding of tumor necrosis factor-like cytokine 1A (TL1A) to death receptor 3 (DR3) plays an important role in the interaction between dendritic cells (DCs) and T cells and contributes to intestinal inflammation development. However, the mechanism by which DCs expressing TL1A mediate helper T (Th) cell differentiation in the intestinal lamina propria (LP) during the pathogenesis of inflammatory bowel disease remains unclear. In this study, we found that TL1A/DR3 promoted Th1 and Th17 cell differentiation in T-T and DC-T cell interaction-dependent manners. TL1A-deficient CD4+ T cells failed to polarize into Th1/Th17 cells and did not cause colonic inflammation in a T cell transfer colitis model. Notably, TL1A was located in the cytoplasm and nuclei of DCs, positively regulated the DC-specific ICAM-grabbing nonintegrin/RAF1/nuclear factor κB signaling pathway, enhanced the antigen uptake ability of DCs, and promoted TLR4-mediated DC activation, inducing naive CD4+ T cell differentiation into Th1 and Th17 cells. Our work reveals that TL1A plays a regulatory role in inflammatory bowel disease pathogenesis.
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Enfermedades Inflamatorias del Intestino , Miembro 15 de la Superfamilia de Ligandos de Factores de Necrosis Tumoral , Humanos , Miembro 15 de la Superfamilia de Ligandos de Factores de Necrosis Tumoral/genética , Miembro 15 de la Superfamilia de Ligandos de Factores de Necrosis Tumoral/metabolismo , Miembro 25 de Receptores de Factores de Necrosis Tumoral/metabolismo , Enfermedades Inflamatorias del Intestino/metabolismo , Inflamación/metabolismo , Factor de Necrosis Tumoral alfaRESUMEN
The influence of global climate change on agricultural productivity is an essential issue of ongoing concern. The growth and development of wheat, maize, and rice are influenced by elevated atmospheric CO2 concentrations, increased temperatures, and seasonal rainfall patterns. However, due to differences in research methodologies (e.g., crop models, climate models, and climate scenarios), there is uncertainty in the existing studies regarding the magnitude and direction of future climate change impacts on crop yields. In order to completely assess the possible consequences of climate change and adaptation measures on crop production and to analyze the associated uncertainties, a database of future crop yield changes was developed using 68 published studies (including 1842 samples). A local polynomial approach was used with the full dataset to investigate the response of crop yield changes to variations in maximum and minimum temperatures, mean temperature, precipitation, and CO2 concentrations. Then, a linear mixed-effects regression model was utilized with the limited dataset to explore the quantitative relationships between them. It was found that maximum temperature, precipitation, adaptation measure, study area, and climate model had significant effects on changes in crop yield. Crop yield will decline by 4.21% for each 1 °C rise in maximum temperature and increase by 0.43% for each 1% rise in precipitation. While higher CO2 concentrations and suitable management strategies could mitigate the negative effects of warming temperatures, crop yield with adaptation measures increased by 64.09% compared to crop yield without adaptation measures. Moreover, the uncertainty of simulations can be decreased by using numerous climate models. The results may be utilized to guide policy regarding the influence of climate change and to promote the creation of adaptation plans that will increase crop systems' resilience in the future.
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The separation and purification of complex and stable stubborn oily sewage is extremely challenging. To respond to this challenge, we developed a powerful flexible filter with ultrahigh strength, durability, flux, separation efficiency, and a multiobjective separation function based on a universal epitaxial growth process of glass fiber fabric (Gf). The underwater oil contact angle (UOCA) of the silicate@Gf (MgSi@Gf) filter is 156.3°, so it can achieve both an ultrahigh permeation flux (5632.7 L·m-2·h-1) and oil-water separation efficiency (99.5%) under gravity (≈ 1 kPa) in purifying surfactant-stabilized emulsions, actual industrial oily sewage and mechanical cold rolling emulsions. The filter with a high tensile strength (66.5 MPa) and oil invasion pressure (4626 Pa) can withstand the impact of much sewage or intense water flow. The filter can tolerate extreme conditions and can maintain high separation performance in acid or alkaline (pH 1-13), high or low temperature (100 °C, 200 °C, -18 °C) conditions or natural salty waters such as seawater. The filter can remove methylene blue (MB) dye (99.8%) by filtration, and can be repeatedly and easily reconstructed (renewable advantage). The filter shows great potential for efficiently eliminating the hazards of contaminants in actual oily sewage and thus protect human health.
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The pathogenesis of inflammatory bowel diseases (IBD) is complex, and dysregulated immune responses play a pivotal role in its occurrence and development. Our previous studies indicated that CD30L may participate in monocyte-mediated inflammation in patients with UC through the activation of circulating monocytes. However, it remains unclear how CD30L participates in monocyte-mediated inflammation in IBD by activation of circulating monocytes. In this study, we observed an increase in the expression of CD30L and chemokine receptor type 2 (CCR2) on circulating monocytes and pro-inflammatory monocytes in the colon lamina propria in mice with dextran sulfate sodium salt (DSS)-induced colitis. Moreover, there was a positive correlation between the expression levels of CCR2 and CD30L (r = 0.8817, p = 0.0480) in monocytes. In Cd30l-/- mice with DSS-induced colitis, the percentage and absolute number of circulating monocytes and pro-inflammatory monocytes decreased with the downregulation of CCR2. Stimulation via CD30L by immobilized anti-CD30L mAb suppressed the expression of pNF-κB p65, pIκBα, p65 and CCR2 and up-regulated the expression of IκBα in the sorted pro-inflammatory monocytes in Cd30l-/- mice with DSS-induced colitis. The mRNA levels of Ccr2 in the sorted pro-inflammatory monocytes were significantly down-regulated with the presence of immobilized RM153 and inhibitors of NF-κB (BAY 11-7082) in WT mice with DSS-induced colitis. Our results suggested that CD30L could promote the inflammatory response by inducing the homing and differentiation of monocytes via the chemokine ligand 2 (CCL2)/CCR2 axis and NF-κB signaling pathway in mice with colitis. These findings provide a novel target for monocyte-based immunotherapy against IBD.
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Ligando CD30/metabolismo , Colitis , Enfermedades Inflamatorias del Intestino , Monocitos/metabolismo , Animales , Quimiocinas/metabolismo , Colitis/metabolismo , Colitis/patología , Sulfato de Dextran , Modelos Animales de Enfermedad , Inflamación/metabolismo , Antígeno Ki-1 , Ligandos , Ratones , Ratones Endogámicos C57BL , FN-kappa B/metabolismo , Receptores CCR2/genética , Receptores CCR2/metabolismo , Receptores de Quimiocina/metabolismoRESUMEN
To explore the effects of different regulation modes on the soil structure and gas transport characteristics in seasonal permafrost regions, freeze-thaw cycles (FTCs) were used as boundary conditions and three typical soils on the Songnen Plain were used: black soil, baijiang soil and meadow soil. Four treatments were established: biochar addition (B1), straw addition (S1), biochar combined with straw addition (B1S1) and an untreated control (CK). The changes in the proportion of soil water-stable aggregates, total soil porosity (TP), soil water characteristic curves (SWRCs), soil dissolved organic carbon (DOC) and soil air permeability (PL) were analyzed. The results showed that biochar and straw influenced the structure of the three soil types. The proportions of large (2-0.5 mm) and medium (0.5-0.25 mm) aggregates increased significantly. The soil aggregate stability indexes of the treated soils were better than those of the CK, and the three-phase ratios of the treated soils were closer to ideal. The different treatments had particularly obvious effects on the black soil; the generalized soil structure index (GSSI) values reached 95.59, 94.36 and 98.74 in the B1, S1 and B1S1 treatments, respectively. An interaction effect was observed between biochar and straw. B1S1 had a stronger effect than the other treatments, and the soil water holding capacity was significantly improved (FC = 0.317 cm3 cm-3). Under the B1S1 treatment, the DOC contents in black soil, baijiang soil and meadow soil were 160.78 mg/kg, 272.828 mg/kg and 271.912 mg/kg, respectively. Moreover, biochar and straw combined effectively reduced PL fluctuations under FTCs and improved the long-term stability of the soil structure. These results can aid in rational straw and biochar use to achieve comprehensive agricultural waste utilization.
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Suelo , Agua , Carbono , Carbón OrgánicoRESUMEN
Greenhouse gas (GHG) emissions from soil carbon and nitrogen cycles during freeze-thaw cycles (FTCs) provide positive feedback to climate warming. Biochar is a new type of soil conditioner that shows potential in soil GHG emissions reduction. To explore the mechanisms of the effects of biochar on soil GHG emissions in seasonally frozen soil areas, this study focused on farmland soil in the Songnen Plain. Variations in soil environmental factors, available carbon and nitrogen and microbial biomass were analyzed using an indoor simulation of soil FTCs. A structural equation model (SEM) was established to reveal the key driving factors and potential mechanism of biochar on soil GHG emissions under FTCs. The results showed that biochar increased carbon dioxide (CO2) emissions by 3.40% and methane (CH4) absorption by 2.52% and decreased nitrous oxide (N2O) emissions by 35.90%. SEM showed that soil temperature (ST) was the main environmental factor determining CO2 emissions and that soil moisture (SM) was the main environmental factor determining CH4 and N2O emissions. Soil available carbon and nitrogen and microbial biomass are important for soil GHG emissions as the reaction substrates and main participants in the biochemical transformation of soil carbon and nitrogen, respectively. This study showed that the application of biochar in farmland is a feasible choice to address climate change in the long term via soil carbon sequestration and GHG emissions reduction. The research results provide a theoretical basis and scientific guidance for soil GHG emissions reduction during FTCs in middle to high latitudes.
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Gases de Efecto Invernadero , Dióxido de Carbono/análisis , Carbón Orgánico , Granjas , Humanos , Metano/análisis , Óxido Nitroso , Suelo/químicaRESUMEN
BACKGROUND: Current therapeutic options for atopic dermatitis (AD) are limited. Janus kinase (JAK) inhibitors may be viable alternatives. OBJECTIVES: To assess the efficacy and safety of JAK inhibitors for AD treatment. METHODS: We searched PubMed, Embase, the Cochrane Controlled Register of Trials, Web of Science, Global Resource of Eczema Trials database, and ClinicalTrials.gov from inception to September 1, 2020. Randomized clinical trials (RCTs) comparing JAK inhibitors with placebo/vehicle treatment for AD patients were included. The primary study outcomes included (1) the change (%) from the Eczema Area and Severity Index (EASI) baseline expressed as weighted mean difference (WMD) and 95% confidence interval (95% CI), and (2) the Investigator's Global Assessment (IGA) response and safety outcomes expressed as relative risk (RR) and 95% CI. RESULTS: We included 14 RCTs published in 13 studies (3,822 patients). Treatment with JAK inhibitors significantly improved IGA response (RR 2.83, 95% CI 2.25-3.56, p < 0.001) and EASI score (WMD -28.82, 95% CI -34.48 to -23.16, p < 0.001). JAK inhibitor treatment achieved the largest improvement in both IGA response (RR 3.59, 95% CI 2.66-4.84, p < 0.001) and EASI score (WMD -42.00, 95% CI -48.64 to -35.36, p < 0.001) by week 4 of treatment. Topical JAK inhibitors were significantly more efficacious than oral inhibitors. Upadacitinib treatment for 4 weeks was most effective in reducing EASI score (WMD -53.92, 95% CI -69.26 to -38.58, p < 0.001), while abrocitinib for 4 weeks led to the most effective IGA response (RR 5.47, 95% CI 2.74-10.93, p < 0.001). There was no difference in the frequency of adverse events (AEs) leading to discontinuation; however, JAK inhibitors use, especially abrocitinib, led to a higher incidence of treatment-emergent AEs (RR 1.25, 95% CI 1.10-1.42, p = 0.001). CONCLUSION: Our results imply that JAK inhibitors are an effective and safe AD treatment. Nevertheless, further trials with longer duration and head-to-head comparisons of different JAK inhibitors are needed.
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Dermatitis Atópica , Eccema , Inhibidores de las Cinasas Janus , Dermatitis Atópica/tratamiento farmacológico , Humanos , Inmunoglobulina A/uso terapéutico , Inhibidores de las Cinasas Janus/efectos adversos , Ensayos Clínicos Controlados Aleatorios como Asunto , Resultado del TratamientoRESUMEN
Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17-producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD.
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Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disease that affects the large intestine and exhibits a relapsing and remitting course. It is a complex immune-mediated disease of the gastrointestinal tract that increases morbidity and negatively influences the quality of life. Although our previous studies have indicated that CD30L is involved in the adaptive immune response in UC, it remains unclear whether it participates in the innate immune response. Our results revealed that the CD30L level significantly increased in the circulating classical monocytes of patients with UC and showed a positive correlation with the severity of UC. CD30L may participate in monocyte-mediated inflammation in patients with UC through the activation of circulating classical monocytes. The present study provides insights into a marker of severity and a potential target for the development of immunological therapy of UC.
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Ligando CD30/inmunología , Colitis Ulcerosa/inmunología , Monocitos/inmunología , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
[This corrects the article DOI: 10.1155/2020/5018975.].
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This study was to identify functions of ILC2, a newly found innate lymphoid cell which mainly locates in mucosa organs like lungs and intestines, in IBD. We injected rIL-33 protein to C57/BL6 mouse to explore how IL-33 induces ILC2 proliferation. The results showed that ILC2 reached a proliferation peak at day 5 and expressed multiple surface markers like CD127, C-kit, CD69, CD44, ST2, CD27, DR3, MHCII, and CD90.2. ILC2 also expressed high quantity of IL-13 and IL-5 and few IL-17A which indicates a potentially immunological function in IBD development. Afterwards, we transferred sort purified ILC2 to Rag1-/- mouse given DSS to induce acute colitis in order to explore the innate function of ILC2. Data showed that ILC2 alleviates DSS-induced acute innate colitis by repairing epithelial barrier and restore body weight. Furthermore, we found that ILC2 can cause macrophages polarizing to M2 macrophages in the gut. Therefore, we concluded that ILC2 played a therapeutic role in mouse acute colitis.
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Colitis/inmunología , Interleucina-33/inmunología , Linfocitos/inmunología , Macrófagos/inmunología , Enfermedad Aguda , Animales , Diferenciación Celular , Proliferación Celular , Células Cultivadas , Citocinas/metabolismo , Modelos Animales de Enfermedad , Proteínas de Homeodominio/genética , Humanos , Transfusión de Linfocitos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Células Th2/inmunologíaRESUMEN
OBJECTIVE: the purpose of this study is to assess the current status of disordered eating attitudes and its related factors among University students in Anhui province, and to provide a basis for health intervention. METHODS: this is a cross-sectional study. The University students admitted to the routine health exams were included in current study, and completed self-administered questionnaires which consist of general information and the Eating Attitudes Test-26 (EAT-26). An EAT-26 score of 20 or higher indicated that a person has disordered eating attitudes. Data were analyzed using the SPSS13.0 software. RESULTS: a total of 1 328 subjects (469 male and 859 female), aged 16-24 years from a university in Wuhu were enrolled in this study. In our survey, the proportion of disordered eating attitudes among male, female, total students were 5.3%, 4.0% and 4.5%, respectively. The proportion of disordered eating attitudes among the students whose family annual income < 10000 RMB, 10,000-30,000 RMB, 30,000-60,000 RMB and > 60,000 were 4.2%, 3.9%, 4.3% and 6.9%, respectively. An interesting finding was that the female students are more likely to have disordered eating attitudes if their parents have more education. CONCLUSIONS: our research reveals that the current status of disordered eating attitudes in Anhui province is relatively low. Parents' education level may be related to eating attitudes among University students. It is essential to increase awareness and understanding of eating disorders and its associated risk factors in University students whose parents have higher education.
Objetivo: el proposito de este estudio es evaluar la situación actual de trastornos alimenticios y sus factores relacionados entre los estudiantes universitarios de la provincia de Anhui, y proporcionar una base para la intervención sanitaria. Métodos: este es un estudio transversal. En el estudio se incluyeron estudiantes universitarios a los que se les realizaron exámenes de salud rutinarios y que completaron cuestionarios que consisten en información general y sobre las actitudes alimentarias: test-26 (EAT-26). Según el test EAT-26, una puntuación de 20 o mayor indica que el sujeto presenta trastornos alimentarios. Los datos fueron analizados mediante el software de SPSS13.0. Resultados: en el estudio se incluyeron un total de 1.328 estudiantes de una universidad de Wuhu (469 hombres y 859 mujeres). En nuestro estudio, la proporción de trastornos alimentarios de hombres y mujeres y el total de estudiantes fueron 5,3%, 4,0%, 4,5%, respectivamente. La proporción de trastornos alimentarios entre los estudiantes cuya familia poseía unos ingresos anuales < 10.000 RMB, 10.000-30.000 RMB, 30.000-60.000 RMB y > 60.000 fueron 4,2%, un 3,9%, 4.3% y 6,9%, respectivamente. Un hallazgo interesante es que los estudiantes son más propensos a padecer trastornos alimentarios si sus padres tienen más educación. Conclusiones: nuestra investigación revela que la situación actual respecto a trastornos alimentarios en la provincia de Anhui es relativamente baja. El nivel de educación de los padres puede ser relacionado con las actitudes de los estudiantes universitarios. Es esencial aumentar la conciencia y la comprensión de los trastornos de la alimentación y sus factores de riesgo asociados con respecto a los estudiantes universitarios cuyos padres tienen una educación superior.
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Trastornos de Alimentación y de la Ingestión de Alimentos/epidemiología , Trastornos de Alimentación y de la Ingestión de Alimentos/psicología , Estudiantes/psicología , Adolescente , Actitud , China/epidemiología , Estudios Transversales , Escolaridad , Femenino , Humanos , Masculino , Padres , Prevalencia , Factores Socioeconómicos , Encuestas y Cuestionarios , Universidades , Adulto JovenRESUMEN
OBJECTIVE: To investigate the temperature change of scrotum caused by varicocele(VC) and its significance. METHODS: One hundred and sixty-six VC patients were examined by infrared ray imaging and colored ultrasonic scanning, and the results were analyzed. Comparisons of the infrared ray images and the routine semen analysis of 106 of the patients were made between before and after the operation. RESULTS: The difference in the infrared ray images between before and after the operation on the VC patients was significant (P < 0.01), and so was the difference in the results of semen analysis(P < 0.01; some of the items P < 0.05). CONCLUSIONS: The infrared ray imaging, together with the semen analysis and colored ultrasonic scanning, had a directive value for the diagnosis and treatment of VC patients.