RESUMEN
This paper has proposed a new thermal wave image sequence compression algorithm by combining double exponential decay fitting model and differential evolution algorithm. This study benchmarked fitting compression results and precision of the proposed method was benchmarked to that of the traditional methods via experiment; it investigated the fitting compression performance under the long time series and improved model and validated the algorithm by practical thermal image sequence compression and reconstruction. The results show that the proposed algorithm is a fast and highly precise infrared image data processing method.
Asunto(s)
Algoritmos , Compresión de Datos/métodos , Aumento de la Imagen/métodos , Interpretación de Imagen Asistida por Computador/métodos , Técnica de Sustracción , Termografía/métodos , Rayos InfrarrojosRESUMEN
A novel blast-inducible RING-H2 type zinc finger protein gene OsRING-1 was cloned from rice by cDNA library screening. OsRING-1 is 1670 bp in length and encodes a 46.6 kDa basic protein with two transmembrane (TM) domains, a basic domain (BD), a conserved domain (CD), a RING finger domain and a serine rich (S-rich) domain. By database search, OsRING-1 was mapped on chromosome 2 and clustered together with other six zinc finger genes. The promoter sequence analysis of OsRING-1 gene revealed that some ABA, GA, ethylene, wound, drought, heat stress and pathogen infection responsive elements were found within the OsRING-1 promoter region. Northern analysis showed that OsRING-1 was induced in different degree by pathogen infections, SA, ABA, JA and ethephon (ET) treatments. Tissue expression analysis showed that OsRING-1 was constitutively strongly expressed in roots, but faintly in stems, leaves and sheaths. Taken together, OsRING-1, as a novel C3H2C3-type zinc finger protein involved in many stress responses in rice might plays a role as a transcription regulator in plant stress response signal transduction pathways.
Asunto(s)
Proteínas de Unión al ADN/genética , Oryza/genética , Dedos de Zinc/genética , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , ADN Complementario/aislamiento & purificación , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Magnaporthe/patogenicidad , Datos de Secuencia Molecular , Proteínas de Plantas/genética , Alineación de Secuencia , Análisis de SecuenciaRESUMEN
BACKGROUND & OBJECTIVE: Proteasome inhibitor is a kind of potential anti-tumor drug,it can induce apoptosis in various tumor cells. This study was designed to investigate the molecular mechanism of apoptosis and G(2)/M arrest in leukemia cell line HL-60 induced by proteasome inhibitor MG132 (Z-Leu-Leu-Leu-CHO). METHODS: Apoptosis in HL-60 cells was observed under fluorescent microscope, flow cytometry and immunoblot were used to analyze cell apoptosis, cell cycle arrest, and the mechanisms. RESULTS: MG132 (2 micromol/L)induced apoptosis in HL-60 cells after 24-h treatment. Meanwhile, HL-60 cells were arrested at G(2)/M phase before apoptosis after induced by MG132. The percentage of G(2)/M phase in MG132-treated HL-60 cells at 12 h was 63.42+/-2.02,while that in untreated cells was 7.29+/-3.01 (P< 0.01). The percentage of apoptosis in MG132-treated HL-60 cells at 24 h was 16.67+/-1.48, while untreated cells had no death (P< 0.01). Compared to the treatment with MG132 only, caffeine (2 mmol/L) exposure can reduce G(2)/M arrest and apoptosis in MG132-treated HL-60 cells. Expression of cyclin-dependent kinase inhibitor p21waf/cip1 up-regulated after treated with MG132 for 3 h, but no p53 or p27 detected. CONCLUSIONS: Proteasome inhibitor MG132 can induce G2/M arrest before the apoptosis appeared in HL-60 cells. The obvious up-regulation of p21 indicated that it is p21(waf/cip1), but not p53 or p53-related proteins,that involved in the regulation of G(2)/M arrest and subsequent apoptosis induced by MG132 in HL-60 cells.
